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产品概述
Dulbecco's 改良培养基--DMEM（Dulbecco's Modified Eagle Medium）是在MEM培养基的基础上研制的，与MEM培养基相比，氨基酸的含量增加了2倍，维生素增加了4倍，同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。DMEM培养基最初设计葡萄糖含量为1000mg/L（低糖型），后来又发展出葡萄糖含量为4500mg/L（高糖型），现已广泛应用于各种细胞的培养。
DMEM高糖型培养基普遍应用于生长快、粘附性低的细胞、杂交瘤的骨髓瘤细胞、克隆细胞、DNA转染的转化细胞、各种原代病毒宿主细胞、单一细胞的培养以及疫苗的生产，例如利用CHO细胞表达EPO和生产乙肝疫苗。
青霉素-链霉素混合液通常也被称为“双抗”，是体外培养中为预防微生物污染最常用的抗生素，其中，青霉素能够干扰细菌细胞壁的合成，对革兰阳性菌特别有效；而链霉素能够与细菌核糖体30S亚单位结合，抑制细菌蛋白质的合成，对革兰氏阴性菌和革兰氏阳性菌均有效，但对革兰氏阴性菌特别有效。青霉素和链霉素联合使用可以预防绝大部分的细菌污染。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分，但不含蛋白质、脂类或任何生长因子，故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
L-谷氨酰胺 | 4 mM |
NaHCO3 | 3700 mg/L |
D-葡萄糖 | 4500 mg/L |
丙酮酸钠 | 1 mM |
HEPES缓冲剂 | 无 |
青霉素G钠盐 | 100 kU/L |
硫酸链霉素 | 100 mg/L |
酚红指示剂 | 15 mg/L |
储存条件 | 2-8℃，避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌，使用时应注意无菌操作，避免污染；
2、为保持本产品的最佳使用效果，请勿进行冻融处理；
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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Maternal androgen excess inhibits fetal cardiomyocytes proliferation through RB-mediated cell cycle arrest and induces cardiac hypertrophy in adulthood (2023-08-29)
作者：Y Huo:1.National Children's Medical Center, Children's Hospital of Fudan University, Fudan University, Shanghai, 201102, China. ; 2.National Health Commission (NHC) Key Laboratory of Neonatal Diseases, Fudan University, 399 Wanyuan Road, Minhang, Shanghai, 201102, China. ; W Wang:1.Guangzhou Center for Disease Control and Prevention, Guangzhou, 510080, China. ; J Zhang:1.National Children's Medical Center, Children's Hospital of Fudan University, Fudan University, Shanghai, 201102, China. ; 2.National Health Commission (NHC) Key Laboratory of Neonatal Diseases, Fudan University, 399 Wanyuan Road, Minhang, Shanghai, 201102, China. ; 3.Institute of Pediatrics, Children's Hospital of Fudan University, Shanghai, 201102, China. ; D Xu:1.National Children's Medical Center, Children's Hospital of Fudan University, Fudan University, Shanghai, 201102, China. ; 2.National Health Commission (NHC) Key Laboratory of Neonatal Diseases, Fudan University, 399 Wanyuan Road, Minhang, Shanghai, 201102
期刊：Maternal androgen excess inhibits fetal cardiomyocytes proliferation through RB-mediated cell cycle arrest and induces cardiac hypertrophy in adulthood
DOI：10.1007/s40618-023-02178-1
影响因子 ：5.4
引用产品： H9c2 (2-1) 细胞 , DMEM高糖 (含双抗) 培养基
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Oral Cancer Stem Cell-Derived Small Extracellular Vesicles Promote M2 Macrophage Polarization and Suppress CD4+ T-Cell Activity by Transferring UCA1 and Targeting LAMC2 (2022-11-28)
作者：Lan Wu:1.Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. ; 2.College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, China. ; 3.National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China. ; Sai Ye:1.Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. ; 2.College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, China. ; 3.National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China. ; Yilin Yao:1.Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. ; 2.College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, China. ; 3.National Clinical Res
期刊：Oral Cancer Stem Cell-Derived Small Extracellular Vesicles Promote M2 Macrophage Polarization and Suppress CD4+ T-Cell Activity by Transferring UCA1 and Targeting LAMC2
影响因子 ：5.1
引用产品： CAL 27 细胞 , 293T [HEK-293T] 细胞 , DMEM高糖 (含双抗) 培养基 , THP-1 细胞 , RPMI-1640完全培养基 (含10%FBS）
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Farrerol suppresses the progression of laryngeal squamous cell carcinoma via the mitochondria-mediated pathway (2011-01-20)
作者：Tingting Zhang:1.Department of Traditional Chinese Medicine, The Affiliated Hospital of Qingdao University, China. ; Hui Liu:1.Department of Traditional Chinese Medicine, The Affiliated Hospital of Qingdao University, China. ; Min Liu:1.Department of Anorectal Surgery, The Affiliated Hospital of Qingdao University, China. ; Chunhong Wang:1.Department of Traditional Chinese Medicine, The Affiliated Hospital of Qingdao University, China. Electronic address: wangchh_chhw@163.com. ;
期刊：Farrerol suppresses the progression of laryngeal squamous cell carcinoma via the mitochondria-mediated pathway
DOI：10.1016/j.ejphar.2021.174636
影响因子 ：5.0
引用产品： DMEM高糖 (含双抗) 培养基
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circHIPK3 (hsa_circ_0000284) Promotes Proliferation, Migration and Invasion of Breast Cancer Cells via miR-326 (2006-01-20)
作者：Liqiang Qi:1.Department of Breast Surgical Oncology, Cancer Institute and Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China. ; Bo Sun:1.The 2nd Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, People's Republic of China. ; Beibei Yang:1.The 2nd Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, People's Republic of China. ; Su Lu:1.The 2nd Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, People's Republic of China. ;
期刊：circHIPK3 (hsa_circ_0000284) Promotes Proliferation, Migration and Invasion of Breast Cancer Cells via miR-326
影响因子 ：4.3
引用产品： DMEM高糖 (含双抗) 培养基 , 特级胎牛血清
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Long non-coding RNA UCA1 relieves cardiomyocytes H9c2 injury aroused by oxygen-glucose deprivation via declining miR-122 (2008-09-20)
作者：Zaiwei Zhang:1.Department of Cardiology, Jining No.1 People's Hospital , Shandong , Jining , China. ; Hu Li:1.Department of Cardiology, Jining No.1 People's Hospital , Shandong , Jining , China. ; Zhiyuan Cui:1.Department of Cardiology, Jining No.1 People's Hospital , Shandong , Jining , China. ; Zhongxing Zhou:1.Department of Cardiology, Jining No.1 People's Hospital , Shandong , Jining , China. ; Shasha Chen:1.Department of Cardiology, Jining No.1 People's Hospital , Shandong , Jining , China. ; Jie Ma:1.Department of Cardiology, Jining No.1 People's Hospital , Shandong , Jining , China. ; Lijuan Hou:1.Department of Cardiology, Jining No.1 People's Hospital , Shandong , Jining , China. ; Xiaoyu Pan:1.Department of Cardiology, Jining No.1 People's Hospital , Shandong , Jining , China. ; Qiaoju Li:1.Department of Painless Endoscopy, Jining No.1 People's Hospital , Shandong , Jining , China. ;
期刊：Long non-coding RNA UCA1 relieves cardiomyocytes H9c2 injury aroused by oxygen-glucose deprivation via declining miR-122
DOI：10.1080/21691401.2019.1652630
影响因子 ：5.8
引用产品： H9c2 (2-1) 细胞 , DMEM高糖 (含双抗) 培养基 , 特级胎牛血清 , DMEM无糖 培养基
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