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产品概述
人卵巢上皮细胞
Cat NO.: CP-H055
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产品名称：人卵巢上皮细胞
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组织来源：卵巢组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人卵巢上皮细胞分离自卵巢组织；卵巢是雌性动物的生殖器官，卵巢的功能是产生卵以及类固醇激素。卵巢的位置与睾丸相同，仅左侧发育(右侧已退化)，呈葡萄状，均为处于不同发育时期的卵泡，卵泡呈黄色，卵巢表面密布血管。卵巢的大小与年龄和产卵期有关。大多数脊椎动物有两个卵巢，但是部分鱼类的两个卵巢融合为单个结构，而所有鸟类只有左侧卵巢有机能。卵巢是位于子宫两侧的一对卵圆形的生殖器官，它的外表有一层上皮组织，其下方有薄层的结缔组织。卵巢的内部结构可分为皮质和髓质。皮质位于卵巢的周围部分，主要由卵泡和结缔组织构成；髓质位于中央，由疏松结缔组织构成，其中有许多血管、淋巴管和神经。卵巢上皮细胞主要功能：①上皮细胞能分泌激素，刺激卵细胞分化成熟；②细胞能分泌炎症介质，参与炎症反应。
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方法简介：
普诺赛实验室分离的人卵巢上皮细胞采用胰蛋白酶-胶原酶联合消化法结合差速贴壁法，并通过上皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人卵巢上皮细胞经Cytokeratin-19免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 鼠尾胶原Ⅰ(2-5μg/cm2) 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H055 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 上皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 人卵巢上皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Identification of prognostic and diagnostic signatures for cancer and acute myocardial infarction: multi-omics approaches for deciphering heterogeneity to enhance patient management (2023-09-14)
作者：Na Yuan:1.The First Hospital of Jiaxing Affiliated Hospitial of Jiaxing University, Jiaxing, Zhejiang, China. ; Hai-Hua Pan:1.The First Hospital of Jiaxing Affiliated Hospitial of Jiaxing University, Jiaxing, Zhejiang, China. ; Yan-Shan Liang:1.Affiliated Dongguan Hospital, Southern Medical University, Dongguan, Guangdong, China. ; Hui-Lin Hu:1.The First Hospital of Jiaxing Affiliated Hospitial of Jiaxing University, Jiaxing, Zhejiang, China. ; Chang-Lin Zhai:1.The First Hospital of Jiaxing Affiliated Hospitial of Jiaxing University, Jiaxing, Zhejiang, China. ; Bo Wang:1.3 The Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China. ;
期刊：Identification of prognostic and diagnostic signatures for cancer and acute myocardial infarction: multi-omics approaches for deciphering heterogeneity to enhance patient management
DOI：10.3389/fphar.2023.1249145
影响因子 ：5.6
引用产品： 人卵巢上皮细胞 , SK-OV-3 细胞
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The role of OIP5 in the carcinogenesis and progression of ovarian cancer (2023-09-02)
作者：Xin Zhang:1.Department of Gynecology and Obstetrics, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui, P.R. China. ; 2.School of Basic Medical Sciences, Wannan Medical College, Wuhu, Anhui, P.R. China. ; Wenjie Gu:1.School of Basic Medical Sciences, Wannan Medical College, Wuhu, Anhui, P.R. China. ; Aiqin Lin:1.School of Basic Medical Sciences, Wannan Medical College, Wuhu, Anhui, P.R. China. ; Renjie Duan:1.School of Basic Medical Sciences, Wannan Medical College, Wuhu, Anhui, P.R. China. ; Likai Lian:1.School of Basic Medical Sciences, Wannan Medical College, Wuhu, Anhui, P.R. China. ; Yuanyuan Huang:1.Department of Gynecology and Obstetrics, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui, P.R. China. ; Tiechen Li:1.School of Basic Medical Sciences, Wannan Medical College, Wuhu, Anhui, P.R. China. tiechenli@126.com. ; Qing Sun:1.Department of Gynecology and Obstetrics, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui, P.R. China. sunqingl@126.com. ;
期刊：The role of OIP5 in the carcinogenesis and progression of ovarian cancer
DOI：10.1186/s13048-023-01265-4
影响因子 ：4.0
引用产品： SK-OV-3 细胞 , A2780 细胞 , 人卵巢上皮细胞
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Phellopterin attenuates ovarian cancer proliferation and chemoresistance by inhibiting the PU.1/CLEC5A/PI3K-AKT feedback loop (2023-09-12)
作者：Aihua Guo:1.Department of Gynecology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou 350014, China. ; Jie Lin:1.Department of Gynecology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou 350014, China. ; Peilin Zhong:1.Department of Gynecology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou 350014, China. ; Jiyun Chen:1.Department of Radiation Oncology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou 350014, China. ; Linghua Wang:1.Department of Gynecology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou 350014, China. ; Xiurong Lin:1.Department of Gynecology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou 350014, China. ; Mei Feng:1.Department of Gynecology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou 350014, Ch
期刊：Phellopterin attenuates ovarian cancer proliferation and chemoresistance by inhibiting the PU.1/CLEC5A/PI3K-AKT feedback loop
DOI：10.1016/j.taap.2023.116691
影响因子 ：3.8
引用产品： 人卵巢上皮细胞
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LINC00936/microRNA-221-3p Regulates Tumor Progression in Ovarian Cancer by Interacting with LAMA3 (2023-02-01)
作者：Chenggan Shu:1.Department of Gynecology, The First People's Hospital of Yancheng, City, Yancheng 224006, P.R. China. ; 2.Department of Laboratory Medicine, Shanghai Tongji Hospital, Shanghai, 200065, P.R. China. ; Weiwei Wang:1.Department of Pathology, Tinghu People's Hospital, Yancheng, 224001, P.R. China. ; Lipei Wu:1.Department of Laboratory Medicine, Dongtai People's Hospital & Dongtai Hospital of Nantong University, Yancheng, 224200, P.R. China. ; Chunrun Qi:1.Department of Pathology, Tinghu People's Hospital, Yancheng, 224001, P.R. China. ; Wenhui Yan:1.Department of Laboratory Medicine, Tinghu People's Hospital, Yancheng, 224001, P.R. China. ; Wenying Lu:1.Department of Laboratory Medicine, Tinghu People's Hospital, Yancheng, 224001, P.R. China. ; Jiale Tian:1.Department of Laboratory Medicine, Shanghai Tongji Hospital, Shanghai, 200065, P.R. China. ; Anquan Shang:1.Department of Laboratory Medicine, Shanghai Tongji Hospital, Shanghai, 200065, P.R. China. ;
期刊：LINC00936/microRNA-221-3p Regulates Tumor Progression in Ovarian Cancer by Interacting with LAMA3
DOI：10.2174/1574892817666220316152201
影响因子 ：2.8
引用产品： SK-OV-3 细胞 , NIH:OVCAR-3 [OVCAR3] 细胞 , A2780 细胞 , HO-8910 细胞 , 人卵巢上皮细胞
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UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization (2003-02-20)
作者：Xi Zeng:1.Department of Obstetrics & Gynecology, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China. ; Wen Zheng:1.Laboratory of Clinical Proteomics and Metabolomics, Institutes for Systems Genetics, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, Sichuan, China. ; Yuting Sheng:1.Department of Obstetrics & Gynecology, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China. ; Hongwei Ma:1.Department of Obstetrics & Gynecology, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China. ;
期刊：UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization
DOI：10.1080/21655979.2022.2048991
影响因子 ：4.9
引用产品： 人卵巢上皮细胞
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P76RBE silencing inhibits ovarian cancer cell proliferation, migration, and invasion via suppressing the integrin β1/NF-κB pathway (2008-01-20)
作者：Limei Yan:1.Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China. ; Zeping He:1.Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China. ; Wei Li:1.Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China. ; Ning Liu:1.Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China. ; Song Gao:1.Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China. ;
期刊：P76RBE silencing inhibits ovarian cancer cell proliferation, migration, and invasion via suppressing the integrin β1/NF-κB pathway
DOI：10.1080/15384101.2021.1963910
影响因子 ：5.2
引用产品： OV-90 细胞 , A2780 细胞 , SK-OV-3 细胞 , NIH:OVCAR-3 [OVCAR3] 细胞 , 人卵巢上皮细胞 , NIH:OVCAR-3 [OVCAR3]细胞专用培养基 , 人卵巢上皮细胞完全培养基 , OV-90细胞专用培养基
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MiR-29c downregulates tumor-expressed B7-H3 to mediate the antitumor NK-cell functions in ovarian cancer (2004-01-20)
作者：Mengqi Deng:1.Department of Gynecologic Oncology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China. Electronic address: 122018000319@mail.ccmu.edu.cn. ; Di Wu:1.Department of Gynecologic Oncology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China. Electronic address: wudi2022@ccmu.edu.cn. ; Yanqin Zhang:1.Department of Gynecologic Oncology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China. Electronic address: 2270934602@qq.com. ; Zhaoyu Jin:1.National Center for Protein Science, Beijing, China. Electronic address: 18610630337@163.com. ; Jinwei Miao:1.Department of Gynecologic Oncology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China. Electronic address: jinweimiao@ccmu.edu.cn. ;
期刊：MiR-29c downregulates tumor-expressed B7-H3 to mediate the antitumor NK-cell functions in ovarian cancer
DOI：10.1016/j.ygyno.2021.04.013
影响因子 ：4.7
引用产品： A2780 细胞 , 人卵巢上皮细胞 , SK-OV-3 细胞 , ES-2 细胞 , NIH:OVCAR-3 [OVCAR3] 细胞
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CircSETDB1 knockdown inhibits the malignant progression of serous ovarian cancer through miR-129-3p-dependent regulation of MAP3K3 (2011-01-20)
作者：Bo Li:1.Department of Gynaecology, Yantaishan Hospital, No.91 Jiefang Road, Zhifu DistrictShandong Province, Yantai, 264001, China. lily122510@126.com. ; Lu Zhang:1.Department of Gynaecology, Yantaishan Hospital, No.91 Jiefang Road, Zhifu DistrictShandong Province, Yantai, 264001, China. ;
期刊：CircSETDB1 knockdown inhibits the malignant progression of serous ovarian cancer through miR-129-3p-dependent regulation of MAP3K3
DOI：10.1186/s13048-021-00875-0
影响因子 ：4.0
引用产品： A2780 细胞 , SK-OV-3 细胞 , 人卵巢上皮细胞 , 293T [HEK-293T] 细胞
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