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产品概述
MEM α是一种改良的MEM培养基,与MEM培养基相比MEM α培养基营养更为丰富,在MEM的基础上又添加了NEAA、丙酮酸钠、硫酸锌、VB12、生物素、抗坏血酸等成分,广泛应用于各种哺乳动物悬浮和贴壁细胞的培养。不含核苷和脱氧核苷的MEM α培养基常常用作DG44和其他DHFR-缺陷型细胞的筛选培养基。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分,但不含蛋白质、脂类或任何生长因子,故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
平衡盐 | Earle’s 盐 |
核苷 | 含 |
L-谷氨酰胺 | 2 mM |
NaHCO3 | 2200 mg/L |
D-葡萄糖 | 1000 mg/L |
丙酮酸钠 | 1 mM |
HEPES缓冲剂 | 无 |
酚红指示剂 | 10 mg/L |
储存条件 | 2-8℃,避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌,使用时应注意无菌操作,避免污染;
2、为保持本产品的最佳使用效果,请勿进行冻融处理;
3、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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Antitumor Effects of Carrier-Free Functionalized Lignin Materials on Human Hepatocellular Carcinoma (HepG2) Cells (2024-01-23)
期刊:ACS Nano
影响因子 :17.1
引用产品: Hep G2 细胞 , 3T3-L1 细胞 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , MEMα 培养基 , DMEM高糖 培养基
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Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway (2024-03-04)
期刊:ACS Nano
影响因子 :17.1
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Capturing cerium ions via hydrogel microspheres promotes vascularization for bone regeneration (2024-01-24)
期刊:Materials Today Bio
DOI:10.1016/j.mtbio.2024.100956
影响因子 :8.2
引用产品: MEMα 培养基 , DMEM高糖 培养基
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Chitosan/NH2-MIL-125 (Ti) scaffold loaded with doxorubicin for postoperative bone tumor clearance and osteogenesis: An in vitro study (2024-02-23)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2024.130368
影响因子 :8.2
引用产品: MEMα 培养基
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YAP represses intestinal inflammation through epigenetic silencing of JMJD3 (2024-01-20)
期刊:Clinical Epigenetics
DOI:10.1186/s13148-024-01626-w
影响因子 :5.7
引用产品: MEMα 培养基
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Regulator of G protein signalling 18 promotes osteocyte proliferation by activating the extracellular signal‑regulated kinase signalling pathway (2024-01-08)
期刊:INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
影响因子 :5.4
引用产品: MLO-Y4 [MLOY4] 细胞 , MEMα 培养基
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Small-molecule inhibitor HI-TOPK-032 improves NK-92MI cell infiltration into ovarian tumours (2024-03-19)
期刊:BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
影响因子 :3.1
引用产品: McCoy's 5A 培养基 , RPMI-1640 培养基 , 青霉素-链霉素溶液 (双抗),100× , MEMα 培养基 , NK-92MI 细胞
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Breadmaking-Inspired Antioxidant Porous Yeast Microcarriers for Stem Cell Delivery in Diabetic Wound Treatment (2023-11-20)
期刊:ADVANCED MATERIALS
影响因子 :29.4
引用产品: MEMα 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , 青霉素-链霉素溶液 (双抗),100×
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A parathyroid hormone related supramolecular peptide for multi-functionalized osteoregeneration (2023-12-27)
期刊:Bioactive Materials
DOI:10.1016/j.bioactmat.2023.12.014
影响因子 :18.9
引用产品: MEMα 培养基 , DMEM高糖 培养基
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An injectable carboxymethyl chitosan hydrogel scaffold formed via coordination bond for antibacterial and osteogenesis in osteomyelitis (2023-10-06)
期刊:CARBOHYDRATE POLYMERS
DOI:10.1016/j.carbpol.2023.121466
影响因子 :11.2
引用产品: MEMα 培养基 , MC3T3-E1 Subclone 24 细胞
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Inducing in situ M2 macrophage polarization to promote the repair of bone defects via scaffold-mediated sustained delivery of luteolin (2023-12-18)
期刊:JOURNAL OF CONTROLLED RELEASE
DOI:10.1016/j.jconrel.2023.11.015
影响因子 :10.8
引用产品: 大鼠骨髓间充质干细胞 , RAW 264.7 细胞 , MEMα 培养基 , DMEM高糖 培养基
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A Self-Adaptive Biomimetic Periosteum Employing Nitric Oxide Release for Augmenting Angiogenesis in Bone Defect Regeneration (2023-11-03)
期刊:Advanced Healthcare Materials
影响因子 :10.0
引用产品: MEMα 培养基 , 青霉素-链霉素溶液 (双抗),100×
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The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications (2023-05-12)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2023.124820
影响因子 :8.2
引用产品: MEMα 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , 0.25%胰蛋白酶溶液 (含EDTA,溶于PBS) , MC3T3-E1 Subclone 14 细胞
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Pyruvate dehydrogenase kinase 1–dependent metabolic reprogramming: A promising target for postmenopausal osteoporosis treatment (2023-02-18)
期刊:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2023.114411
影响因子 :7.5
引用产品: MEMα 培养基
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Endoplasmic reticulum stress mediated by ROS participates in cadmium exposure-induced MC3T3-E1 cell apoptosis (2023-01-18)
期刊:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI:10.1016/j.ecoenv.2023.114517
影响因子 :6.8
引用产品: MEMα 培养基
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Application of steam explosion treatment on the collagen peptides extraction from cattle bone (2023-03-09)
期刊:Innovative Food Science & Emerging Technologies
DOI:10.1016/j.ifset.2023.103336
影响因子 :6.6
引用产品: MC3T3-E1 Subclone 24 细胞 , MEMα 培养基 , 特级胎牛血清
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Titanium surface-grafted zwitterionic polymers with an anti-polyelectrolyte effect enhances osteogenesis (2023-04-03)
期刊:COLLOIDS AND SURFACES B-BIOINTERFACES
DOI:10.1016/j.colsurfb.2023.113293
影响因子 :5.8
引用产品: 特级胎牛血清 , MEMα 培养基 , 0.25%胰蛋白酶溶液 (含EDTA,溶于PBS) , 青霉素-链霉素溶液 (双抗),100×
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Biocompatibility and osteoinductive ability of casein phosphopeptide modified polyetheretherketone (2023-02-08)
期刊:Frontiers in Bioengineering and Biotechnology
DOI:10.3389/fbioe.2023.1100238
影响因子 :5.7
引用产品: MEMα 培养基
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Morroniside ameliorates glucocorticoid-induced osteoporosis and promotes osteoblastogenesis by interacting with sodium-glucose cotransporter 2 (2023-02-14)
期刊:PHARMACEUTICAL BIOLOGY
DOI:10.1080/13880209.2023.2173787
影响因子 :3.8
引用产品: MC3T3-E1 Subclone 14 细胞 , MEMα 培养基
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The initial application of conch-shell hydroxyapatite and HACC as functional filler in PMMA bone cement (2023-08-06)
期刊:International Journal of Polymeric Materials and Polymeric Biomaterials
DOI:10.1080/00914037.2023.2243367
影响因子 :3.2
引用产品: MC3T3-E1 Subclone 14 细胞 , MEMα 培养基 , MC3T3-E1 Subclone 14 成骨诱导分化培养基
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Study on the mechanism of NLRP3 effect on the skeleton of de-ovalized mice (2023-06-10)
期刊:Biochemistry and Biophysics Reports
DOI:10.1016/j.bbrep.2023.101496
影响因子 :2.7
引用产品: MEMα 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , 小鼠骨髓间充质干细胞
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Enhanced biocompatibility and osteogenic ability on amino-modified polyetheretherketone (2023-01-31)
期刊:Materials Research Express
影响因子 :2.3
引用产品: MEMα 培养基
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Preparation of a cattle bone collagen peptide–calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells (2022-12-15)
期刊:Food & Function
影响因子 :6.3
引用产品: MC3T3-E1 Subclone 24 细胞 , MEMα 培养基 , 特级胎牛血清 , 小鼠骨髓间充质干细胞成骨诱导分化培养基
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Betamethasone-loaded dissolvable microneedle patch for oral ulcer treatment (2022-12-16)
期刊:COLLOIDS AND SURFACES B-BIOINTERFACES
DOI:10.1016/j.colsurfb.2022.113100
影响因子 :6.0
引用产品: MEMα 培养基
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Resveratrol Ameliorates High Altitude Hypoxia-Induced Osteoporosis by Suppressing the ROS/HIF Signaling Pathway (2022-08-28)
期刊:MOLECULES
影响因子 :4.9
引用产品: MEMα 培养基
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Macrophage M1 polarization mediated via the IL-6/STAT3 pathway contributes to apical periodontitis induced by Porphyromonas gingivalis (2022-11-21)
期刊:Journal of Applied Oral Science
DOI:10.1590/1678-7757-2022-0316
影响因子 :3.1
引用产品: MEMα 培养基 , DMEM高糖 培养基
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Nanobody-Engineered Natural Killer Cell Conjugates for Solid Tumor Adoptive Immunotherapy (2010-01-20)
期刊:Small
影响因子 :13.3
引用产品: MEMα 培养基
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Angiogenic Actions of Paeoniflorin on Endothelial Progenitor Cells and in Ischemic Stroke Rat Model (2004-01-20)
期刊:American Journal Of Chinese Medicine
影响因子 :6.0
引用产品: 大鼠骨髓来源内皮祖细胞完全培养基 , MEMα 培养基
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Bilayer Membrane Composed of Mineralized Collagen and Chitosan Cast Film Coated With Berberine-Loaded PCL/PVP Electrospun Nanofiber Promotes Bone Regeneration (2007-01-20)
期刊:Frontiers in Bioengineering and Biotechnology
影响因子 :5.7
引用产品: MC3T3-E1 Subclone 14 细胞 , MEMα 培养基
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Role of vasodilator‑stimulated phosphoprotein in RANKL‑differentiated murine macrophage RAW264.7 cells: Modulation of NF‑κB, c‑Fos and NFATc1 transcription factors (2002-01-20)
期刊:Experimental and Therapeutic Medicine
影响因子 :2.7
引用产品: MEMα 培养基
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Fabrication and Characterization of Suspension Plasma-Sprayed Fluoridated Hydroxyapatite Coatings for Biomedical Applications (2008-08-20)
期刊:J Therm Spray Tech
影响因子 :3.1
引用产品: MC3T3-E1 Subclone 14 细胞 , 磷酸盐缓冲液 (PBS) 1× , MEMα 培养基
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Preparation, physicochemical properties and biocompatibility of PBLG/PLGA/bioglass composite scaffolds (2009-06-20)
期刊:Materials Science & Engineering C-Materials for Biological Applications
DOI:10.1016/j.msec.2016.09.085
影响因子 :7.9
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Physicochemical properties and biocompatibility of PZL/PLGA/bioglass composite scaffolds for bone tissue engineering (2010-06-20)
期刊:RSC Advances
影响因子 :3.9
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Preparation and characterization of foamy poly(γ-benzyl- l -glutamate- co - l -phenylalanine)/bioglass composite scaffolds for bone tissue engineering (2007-06-20)
期刊:RSC Advances
影响因子 :3.9
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