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| 名称 | NK-92MI (人恶性非霍奇金淋巴瘤患者的自然杀伤细胞) (STR鉴定正确) |
| 别称 | NK-92 MI; NK-92 mi; NK92-MI; NK92MI; NK-92 transfected with MFG-Hil2 |
| 种属 | 人类 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 淋巴母细胞样 |
| 冻存条件 | 冻存液：90%FBS+10%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
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| 推荐传代比例 | 5×10^5-1×10^6cells/mL |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 该细胞为悬浮细胞，请注意离心收集细胞悬液；请勿直接倒掉细胞培养液。 |
| 背景描述 | NK-92细胞是从一位患有急进性非霍奇金淋巴瘤的50岁白人男性外周血单核细胞衍生来的一株IL-2依赖型NK细胞株。NK-92MI细胞是转染得到的源自NK-92细胞的IL-2非依赖的NK细胞株。亲本细胞NK-92通过微粒体基因转化法用逆转录病毒MFG-hIL-2载体携带的人IL-2cDNA进行转化。可能由于载体整合到基因组DNA中，转化是稳定的。这株细胞对很多恶性细胞有细胞毒性；铬释放试验显示它能杀死K562细胞和Daudi细胞。NK-92细胞有以下特征：CD2、CD7、CD11a、CD28、CD45、CD54表面标记阳性；CD1、CD3、CD4、CD5、CD8、CD10、CD14、CD16、CD19、CD20、CD23、CD34和HLA-DR表面标记阴性。其亲本IL-2依赖的细胞株NK-92细胞及另一株同样来源于NK-92细胞株的IL-2非依赖的细胞株NK-92CI都可从ATCC得到。NK-92MI细胞和NK-92CI细胞这两个变种都包含、表达并合成hIL-2cDNA。NK-92MI细胞合成的IL-2水平比NK-92CI高，而亲本细胞不合成表达。1998年9月提交到ATCC的培养物污染了支原体，其后代通过BM细胞周期蛋白处理21天消除支原体。处理后6周，用Hoechst染色、PCR和标准培养测试进行支原体检测，结果都呈阴性。 |
| 年龄（性别） | 男性；50岁 |
| 组织来源 | 外周血 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 淋巴瘤细胞 |
| 生物安全等级 | BSL-2 |
| 保藏机构 | ATCC; CRL-2408 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 19,20 D3S1358 15 D5S818 12,13 D7S820 10,11 D8S1179 12 D13S317 9,12 D16S539 11,12 D18S51 12,17 D19S433 14,15 D21S11 31.2,32 FGA 20,22 PentaD 10,12 PentaE 12 TH01 6,9.3 TPOX 8 vWA 18 D6S1043 11,12 D12S391 19 D2S441 14 -
STR鉴定图
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参考文献
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Small-molecule inhibitor HI-TOPK-032 improves NK-92MI cell infiltration into ovarian tumours (2024-03-19)
作者：Mengqi Deng:1.Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China. ; Ruiye Yang:1.Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China. ; Qi Sun:1.Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China. ; Jiamin Zhang:1.Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China. ; Jinwei Miao:1.Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China. ;
期刊：Small-molecule inhibitor HI-TOPK-032 improves NK-92MI cell infiltration into ovarian tumours
影响因子 ：3.1
引用产品： McCoy's 5A 培养基 , RPMI-1640 培养基 , 青霉素-链霉素溶液 (双抗)，100× , MEMα 培养基 , NK-92MI 细胞
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NK cell-derived exosomes enhance the anti-tumor effects against ovarian cancer by delivering cisplatin and reactivating NK cell functions (2023-01-19)
作者：Heyong Luo:1.Tissue Engineering and Stem Cell Experiment Center, Guizhou Medical University (GMU), Guiyang, Guizhou, China. ; 2.Department of Immunology, College of Basic Medical Sciences, Guizhou Medical University, Guiyang, Guizhou, China. ; Yanhua Zhou:1.Tissue Engineering and Stem Cell Experiment Center, Guizhou Medical University (GMU), Guiyang, Guizhou, China. ; Jing Zhang:1.Department of Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, Guizhou, China. ; Yingchun Zhang:1.Department of Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, Guizhou, China. ; Shiqi Long:1.Department of Immunology, College of Basic Medical Sciences, Guizhou Medical University, Guiyang, Guizhou, China. ; Xiaojin Lin:1.Tissue Engineering and Stem Cell Experiment Center, Guizhou Medical University (GMU), Guiyang, Guizhou, China. ; 2.Department of Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, Guizhou, China. ;
期刊：NK cell-derived exosomes enhance the anti-tumor effects against ovarian cancer by delivering cisplatin and reactivating NK cell functions
DOI：10.3389/fimmu.2022.1087689
影响因子 ：7.3
引用产品： IOSE80 细胞 , NK-92MI 细胞
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Single-cell profiling reveals immune disturbances landscape and HLA-F-mediated immune tolerance at the maternal-fetal interface in preeclampsia (2023-10-03)
作者：Fangyuan Luo:1.Department of Obstetrics and Gynecology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology, Chengdu, China. ; 2.Department of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, Chengdu, China. ; 3.Department of Obstetrics/Gynecology, Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital of Sichuan University, Chengdu, China. ; Fulin Liu:1.Department of Obstetrics/Gynecology, Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital of Sichuan University, Chengdu, China. ; Yingzhe Guo:1.School of Medical and Life Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu, China. ; Wenming Xu:1.Department of Obstetrics/Gynecology, Key Laboratory of Obstetric, Gynecologic an
期刊：Single-cell profiling reveals immune disturbances landscape and HLA-F-mediated immune tolerance at the maternal-fetal interface in preeclampsia
DOI：10.3389/fimmu.2023.1234577
影响因子 ：7.3
引用产品： JAR 细胞 , NK-92MI 细胞
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Inhibitory effect and related mechanism of decitabine combined with gemcitabine on proliferation of NK/T cell lymphoma cells (2023-03-01)
作者：Lanke Lin:1.College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu, China. ; Xiangqin Liu:1.Department of Laboratory Medicine and Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China. ; Hui Yu:1.Department of Laboratory Medicine, The People's Hospital of Leshan, Leshan, China. ; Huan Deng:1.College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu, China. ; Kun Peng:1.Health Management Center, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China. ; Jiang Chen:1.The Department of Ophthalmology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China. ; Chunle Zhang:1.Division of Nephrology, Kidney Research Institute, West China Hospital of Sichuan University, Chengdu, China. ; Tao
期刊：Inhibitory effect and related mechanism of decitabine combined with gemcitabine on proliferation of NK/T cell lymphoma cells
DOI：10.3389/fphar.2023.1134895
影响因子 ：5.6
引用产品： NK-92MI 细胞
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EP3 enhances adhesion and cytotoxicity of NK cells toward hepatic stellate cells in a murine liver fibrosis model (2005-02-20)
作者：Xixi Tao:1.Department of Pharmacology, Tianjin Key Laboratory of Inflammatory Biology, Center for Cardiovascular Diseases, Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China. ; Rui Zhang:1.Department of Pharmacology, Tianjin Key Laboratory of Inflammatory Biology, Center for Cardiovascular Diseases, Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China. ; Ronglu Du:1.Department of Pharmacology, Tianjin Key Laboratory of Inflammatory Biology, Center for Cardiovascular Diseases, Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), The Province and Ministry Co-sponsored
期刊：EP3 enhances adhesion and cytotoxicity of NK cells toward hepatic stellate cells in a murine liver fibrosis model
影响因子 ：15.3
引用产品： NK-92MI 细胞
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Bufalin enhances the killing efficacy of NK cells against hepatocellular carcinoma by inhibiting MICA shedding (2010-01-20)
作者：Rao Fu:1.The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning, China; Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China. ; Fangjing Yu:1.The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning, China; Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China. ; Weiqi Wu:1.The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning, China; Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China. ; Juan Liu:1.The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning, China. ; Jia Li:1.The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning, China. ; Fangyue Guo:1.The First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning, China; Laboratory of Integrative Medicine, The First Affiliated
期刊：Bufalin enhances the killing efficacy of NK cells against hepatocellular carcinoma by inhibiting MICA shedding
DOI：10.1016/j.intimp.2021.108195
影响因子 ：5.7
引用产品： SK-Hep-1 细胞 , Hep G2 细胞 , NK-92MI 细胞 , NK-92MI细胞专用培养基
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A novel human anti-TIGIT monoclonal antibody with excellent function in eliciting NK cell-mediated antitumor immunity (2001-01-20)
作者：Dong Han:1.Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China. ; Yinfeng Xu:1.Beijing Institute of Biotechnology, Beijing, China. ; Xinping Zhao:1.Beijing Institute of Biotechnology, Beijing, China; PLA Center for Disease Control and Prevention, Beijing, China. ; Yunyun Mao:1.Beijing Institute of Biotechnology, Beijing, China. ; Qinglin Kang:1.Beijing Institute of Biotechnology, Beijing, China. ; Weihong Wen:1.Institute of Medical Research, Northwestern Polytechnical University, Xi'an, China. ; Xiaoyan Yu:1.Beijing Institute of Biotechnology, Beijing, China. ; Lei Xu:1.Beijing Institute of Biotechnology, Beijing, China. ; Fujia Liu:1.Beijing Institute of Biotechnology, Beijing, China. ; Mengyao Zhang:1.Beijing Institute of Biotechnology, Beijing, China. ; Jiazhen Cui:1.Beijing Institute of Biotechnology, Beijing, China. ; Zhang Wang:1.Beijing Institute of Biotechnology, Beijing, China; Hainan Medical University, Haikou, China. ; Zhixin
期刊：A novel human anti-TIGIT monoclonal antibody with excellent function in eliciting NK cell-mediated antitumor immunity
DOI：10.1016/j.bbrc.2020.12.013
影响因子 ：3.1
引用产品： NK-92MI 细胞
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Acute lymphoblastic leukemia‑derived exosome inhibits cytotoxicity of natural killer cells by TGF‑β signaling pathway (2006-01-20)
作者：Huijun Yu:1.Shenzhen Maternal and Child Healthcare Hospital, The First School of Clinical Medicine, Southern Medical University, 2004 Hongli road, Futian District, Shenzhen, Guangdong China. ; 2.Clinical Laboratory Medicine Center, Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong China. ; Tingting Huang:1.Clinical Laboratory Medicine Center, Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong China. ; Daming Wang:1.Clinical Laboratory Medicine Center, Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong China. ; Lei Chen:1.Clinical Laboratory Medicine Center, Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong China. ; Xi Lan:1.Clinical Laboratory Medicine Center, Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong China. ; Xintong Liu:1.Clinical Laboratory Medicine Center, Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong China. ; Keyan Chen:1.Clinical Laboratory Medicine Center,
期刊：Acute lymphoblastic leukemia‑derived exosome inhibits cytotoxicity of natural killer cells by TGF‑β signaling pathway
DOI：10.1007/s13205-021-02817-5
影响因子 ：2.8
引用产品： Jurkat, Clone E6-1 细胞 , NK-92MI 细胞
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