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产品概述
名称 | K-562 [K562] (人慢性髓原白血病细胞) (STR鉴定正确) |
别称 | K562; K 562; GM05372 |
种属 | 人 |
生长特性 | 悬浮细胞 |
细胞形态 | 淋巴细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
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推荐传代比例 | 3×10^5-5×10^5cells/mL |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。 |
背景描述 | 连续细胞株K-562是由Lozzio从一位临终的53岁女性慢性骨髓性白血病患者的胸水中建立。K-562细胞被归入高度未分化的粒性白细胞类,Erson等对其表面膜性质的研究表明,K-562细胞是一株人类红白血病细胞。K-562细胞在自然杀伤分析中广泛作为高敏感的体外受体,See Pross等将K-562细胞用于NK细胞的体外详细检测,包括NK细胞活性的数学量化。K-562母细胞是多能性造血恶性细胞,它能自发分化成可识别的红血球祖细胞、粒性白细胞、单核细胞等。K-562细胞是EBNA阴性的。 |
年龄(性别) | 女性;53岁 |
组织来源 | 骨髓;慢性骨髓性白血病(CML) |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 白血病细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~30-48 hours |
致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
保藏机构 | ATCC; CCL-243 BCRC; 60007 BCRJ; 0126 CCLV; CCLV-RIE 0439 DSMZ; ACC-10 ECACC; 89121407 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 9,10 D2S1338 17 D3S1358 16 D5S818 11,12 D7S820 9,11 D8S1179 12 D13S317 8 D16S539 11,12 D18S51 15,16 D19S433 14,14.2 D21S11 29,31 FGA 21,24 PentaD 9,13 PentaE 5,14 TH01 9.3 TPOX 8,9 vWA 16 D6S1043 11 D12S391 23 D2S441 10,14 -
STR鉴定图
参考文献
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A Divalent Chikungunya and Zika Nanovaccine with Thermostable Self-assembly Multivalent Scaffold LS-SUMO (2024-02-10)
作者:Qianlin Li:1.Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China. ; 2.One Health Center of Excellence for Research and Training, School of Public Health, Sun Yat-sen University, Guangzhou, 510080, China. ; Jinsong Zhang:1.One Health Center of Excellence for Research and Training, School of Public Health, Sun Yat-sen University, Guangzhou, 510080, China. ; Qiang Deng:1.One Health Center of Excellence for Research and Training, School of Public Health, Sun Yat-sen University, Guangzhou, 510080, China. ; Conghui Liao:1.One Health Center of Excellence for Research and Training, School of Public Health, Sun Yat-sen University, Guangzhou, 510080, China. ; Jun Qian:1.Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China. ; Zeliang Chen:1.One Health Center of Excellence for Research and Training, School of Public Health, Sun Yat-sen University, Guangzhou, 510080, China. ; Jiahai Lu:1.One Health Center of Excellence for Research and Trai
期刊:A Divalent Chikungunya and Zika Nanovaccine with Thermostable Self-assembly Multivalent Scaffold LS-SUMO
影响因子 :10.0
引用产品: K-562 [K562] 细胞 , IMDM 培养基
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Selection of reference genes in liproxstatin-1-treated K562 Leukemia cells via RT-qPCR and RNA sequencing (2024-01-02)
作者:Hai-Qun Dong:1.Department of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China. ; Xue-Ying Hu:1.Department of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China. ; Shi-Jing Liang:1.Department of Hematology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China. ; 2.Key Laboratory of Hematology, Guangxi Medical University, Education Department of Guangxi Zhuang Autonomous Region, Nanning, 530021, China. ; Ren-Sheng Wang:1.Department of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China. 13807806008@163.com. ; Peng Cheng:1.Department of Hematology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China. gxchengpeng@163.com. ; 2.Key Laboratory of Hematology, Guangxi Medical University, Education Department of Guangxi Zhuang Autonomous Region, Nanning, 530021, China. gxc
期刊:Selection of reference genes in liproxstatin-1-treated K562 Leukemia cells via RT-qPCR and RNA sequencing
DOI:10.1007/s11033-023-08912-5
影响因子 :2.8
引用产品: K-562 [K562] 细胞
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Label-free, rapid and highly accurate identification and categorization of leukemia cells via Raman spectroscopy (2023-08-22)
作者:Electronic Materials Research Laboratory, Key Laboratory of the Ministry of Education & International Center for Dielectric Research, School of Electronic Science and Engineering, Xi’an Jiaotong University, Xi’an, China Biology Program, Faculty of Science, The University of British Columbia, Vancouver, BC V6T 1Z4, Canada
期刊:Label-free, rapid and highly accurate identification and categorization of leukemia cells via Raman spectroscopy
影响因子 :8.4
引用产品: K-562 [K562] 细胞 , U-937 细胞 , Raji 细胞 , Jurkat, Clone E6-1 细胞 , K-562 [K562]细胞专用培养基 , U-937细胞专用培养基 , Raji细胞专用培养基 , Jurkat, Clone E6-1细胞专用培养基
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Development and evaluation of novel artemisinin-isatin hybrids with potential anti-leukemic cytotoxicity (2023-04-14)
作者:Peng Wang:1.Department of Critical Care Unit, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. ; Zhe Zhang:1.Department of Tumor Radiotherapy, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. ; Wei Cao:1.Department of Nephrology, The First Affiliated Hospital of Shandong First Medical University & Provincial Qianfoshan Hospital, Shandong Institute of Nephrology, Jinan, Shandong, China. ; Xuan Zhang:1.Department of Geriatric Respiratory Disease, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. ;
期刊:Development and evaluation of novel artemisinin-isatin hybrids with potential anti-leukemic cytotoxicity
影响因子 :4.7
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Ester tethered artemisinin-isatin hybrids: design, synthesis and anti-leukemic activity evaluation (2023-08-21)
作者:Peng Wang: 1.The First Clinical College, Shandong University of Traditional Chinese Medicine, **an, China;2.Department of Critical Care Unit, Shandong Provincial Hospital Affiliated to Shandong First Medical University, **an, China; Weiwei Wang: 1.The First Clinical College, Shandong University of Traditional Chinese Medicine, **an, China; Lei Zhou: 1.The First Clinical College, Shandong University of Traditional Chinese Medicine, **an, China; Lin Wang: 1.The First Clinical College, Shandong University of Traditional Chinese Medicine, **an, China; Yang Zhao: 1.The First Clinical College, Shandong University of Traditional Chinese Medicine, **an, China; Jiarui Xu: 1.Department of Critical Care Unit, Shandong Provincial Hospital Affiliated to Shandong First Medical University, **an, China; Dai Yuan: 1.Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, **an, China; Li Kong: 1.Department of Emergency Center, Shandong University of Tradit
期刊:Ester tethered artemisinin-isatin hybrids: design, synthesis and anti-leukemic activity evaluation
DOI:10.1007/s00044-023-03122-x
影响因子 :2.6
引用产品: K-562 [K562] 细胞 , K562/ADR 细胞 , CCRF-CEM [CCRF CEM] 细胞 , 人外周血单核细胞
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Pentanoate-Tethered Artemisinin-Isatin Hybrids with Antileukemic Potential (2023-03-21)
作者:School of Pharmacy, Guizhou University of Traditional Chinese Medicine, 550025 Guiyang, Guizhou, PR China
期刊:Pentanoate-Tethered Artemisinin-Isatin Hybrids with Antileukemic Potential
影响因子 :2.1
引用产品: K-562 [K562] 细胞 , K562/ADR 细胞 , CCRF-CEM [CCRF CEM] 细胞
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Tet2-mediated DNA demethylation regulates the proliferation and apoptosis of human leukemia K562 cells (2023-03-31)
作者:Yan Qiao:1.Guizhou Provincial Key Laboratory for Regenerative Medicine, Stem Cell and Tissue Engineering Research Center, Guizhou Medical University, Guiyang, Guizhou 550005, China. 1716324799@qq.com. ; Yanhua Zhou:1.Guizhou Provincial Key Laboratory for Regenerative Medicine, Stem Cell and Tissue Engineering Research Center, Guizhou Medical University, Guiyang, Guizhou 550005, China. 36088035@qq.com. ; Honglan Yang:1.Guizhou Provincial Key Laboratory for Regenerative Medicine, Stem Cell and Tissue Engineering Research Center, Guizhou Medical University, Guiyang, Guizhou 550005, China. 1064589342@qq.com. ; Zhixu He:1.Guizhou Provincial Key Laboratory for Regenerative Medicine, Stem Cell and Tissue Engineering Research Center, Guizhou Medical University, Guiyang, Guizhou 550005, China. hzx@ygmc.edu.cn. ; Anran Fan:1.Guizhou Provincial Key Laboratory for Regenerative Medicine, Stem Cell and Tissue Engineering Research Center, Guizhou Medical University, Guiyang, Guizhou 550005, China
期刊:Tet2-mediated DNA demethylation regulates the proliferation and apoptosis of human leukemia K562 cells
影响因子 :1.6
引用产品: K-562 [K562] 细胞
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Liproxstatin‑1 induces cell cycle arrest, apoptosis, and caspase‑3/GSDME‑dependent secondary pyroptosis in K562 cells (2022-08-17)
作者:Hai-Qun Dong:1.Department of Hematology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China. ; Shi-Jing Liang:1.Department of Hematology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China. ; Yu-Ling Xu:1.Department of Hematology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China. ; Yi Dai:1.Department of Hematology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China. ; Na Sun:1.Department of Hematology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China. ; Dong-Hong Deng:1.Department of Hematology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China. ; Peng Che
期刊:Liproxstatin‑1 induces cell cycle arrest, apoptosis, and caspase‑3/GSDME‑dependent secondary pyroptosis in K562 cells
影响因子 :5.9
引用产品: K-562 [K562] 细胞 , SH-SY5Y [SHSY-5Y] 细胞
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Lipid droplets as endogenous intracellular microlenses (2012-01-20)
作者:Xixi Chen:1.Institute of Nanophotonics, Jinan University, 511443, Guangzhou, China. ; Tianli Wu:1.Institute of Nanophotonics, Jinan University, 511443, Guangzhou, China. ; Zhiyong Gong:1.Institute of Nanophotonics, Jinan University, 511443, Guangzhou, China. ; Jinghui Guo:1.Department of Physiology, School of Medicine, Jinan University, 510632, Guangzhou, China. ; Xiaoshuai Liu:1.Institute of Nanophotonics, Jinan University, 511443, Guangzhou, China. ; Yao Zhang:1.Institute of Nanophotonics, Jinan University, 511443, Guangzhou, China. zhyao5@jnu.edu.cn. ; Yuchao Li:1.Institute of Nanophotonics, Jinan University, 511443, Guangzhou, China. liyuchao@jnu.edu.cn. ; Pietro Ferraro:1.CNR-ISASI, Institute of Applied Sciences and Intelligent Systems «E. Caianiello», Via Campi Flegrei 34, 80078, Pozzuoli, Naples, Italy. pietro.ferraro@cnr.it. ; Baojun Li:1.Institute of Nanophotonics, Jinan University, 511443, Guangzhou, China. baojunli@jnu.edu.cn. ;
期刊:Lipid droplets as endogenous intracellular microlenses
DOI:10.1038/s41377-021-00687-3
影响因子 :19.4
引用产品: K-562 [K562] 细胞
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CXCR5 guides migration and tumor eradication of anti-EGFR chimeric antigen receptor T cells (2007-01-20)
作者:Guangchao Li:1.Guangzhou Bio-gene Technology Co., Ltd., Guangzhou, Guangdong Province, China. ; Jintao Guo:1.Guangzhou Bio-gene Technology Co., Ltd., Guangzhou, Guangdong Province, China. ; Yanfang Zheng:1.Department of Oncology, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong Province, China. ; Wen Ding:1.Guangzhou Bio-gene Technology Co., Ltd., Guangzhou, Guangdong Province, China. ; Zheping Han:1.Department of Laboratory Medicine, Central Hospital of Panyu District, Guangzhou, Guangdong Province, China. ; Lingyu Qin:1.Department of Oncology, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong Province, China. ; Wenjun Mo:1.Guangzhou Bio-gene Technology Co., Ltd., Guangzhou, Guangdong Province, China. ; Min Luo:1.Guangzhou Bio-gene Technology Co., Ltd., Guangzhou, Guangdong Province, China. ;
期刊:CXCR5 guides migration and tumor eradication of anti-EGFR chimeric antigen receptor T cells
DOI:10.1016/j.omto.2021.07.003
影响因子 :5.7
引用产品: A549/DDP 细胞 , COS-1 细胞 , K-562 [K562] 细胞 , NCI-H929 细胞
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Human m6A-mRNA and lncRNA epitranscriptomic microarray reveal function of RNA methylation in hemoglobin H-constant spring disease (2010-01-20)
作者:Heyun Ruan:1.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, Guangxi, China. ; 2.Department of Obstetrics and Gynecology, Minzu Hospital of Guangxi, Zhuang Autonomous Region, Affiliated Minzu Hospital of Guangxi Medical University, Nanning, Guangxi, China. ; Fang Yang:1.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, Guangxi, China. ; Lingjie Deng:1.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, Guangxi, China. ; Dongmei Yang:1.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, Guangxi, China. ; Xiaoli Zhang:1.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, Guan
期刊:Human m6A-mRNA and lncRNA epitranscriptomic microarray reveal function of RNA methylation in hemoglobin H-constant spring disease
DOI:10.1038/s41598-021-99867-9
影响因子 :4.6
引用产品: K-562 [K562] 细胞
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A novel upregulated LncRNA-AC026150.8 promotes chemo-resistance and predicts poor prognosis in acute myeloid leukemia (2010-01-20)
作者:Henan Zhang:1.Hematology Laboratory, Shengjing Hospital of China Medical University, Shenyang, China. ; Yue Zhao:1.Hematology Laboratory, Shengjing Hospital of China Medical University, Shenyang, China. ; Xuan Liu:1.Hematology Laboratory, Shengjing Hospital of China Medical University, Shenyang, China. ; Yusi Liu:1.Hematology Laboratory, Shengjing Hospital of China Medical University, Shenyang, China. ; Xiaohui Wang:1.Hematology Laboratory, Shengjing Hospital of China Medical University, Shenyang, China. ; Yu Fu:1.Hematology Laboratory, Shengjing Hospital of China Medical University, Shenyang, China. ; Shuang Fu:1.Hematology Laboratory, Shengjing Hospital of China Medical University, Shenyang, China. ; Jihong Zhang:1.Hematology Laboratory, Shengjing Hospital of China Medical University, Shenyang, China. ;
期刊:A novel upregulated LncRNA-AC026150.8 promotes chemo-resistance and predicts poor prognosis in acute myeloid leukemia
影响因子 :4.0
引用产品: KG-1 细胞 , K-562 [K562] 细胞
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TXNRD1: A Key Regulator Involved in the Ferroptosis of CML Cells Induced by Cysteine Depletion In Vitro (2012-01-20)
作者:Shuhan Liu:1.Department of Laboratory Medicine, The School of Medical Technology and Engineering, Fujian Medical University, Fujian, China 350004. ; Wei Wu:1.Department of Laboratory Medicine, The School of Medical Technology and Engineering, Fujian Medical University, Fujian, China 350004. ; 2.Medical Technology Experimental Teaching Center, The School of Medical Technology and Engineering, Fujian Medical University, Fujian, China 350004. ; Qiaoqian Chen:1.Department of Laboratory Medicine, The School of Medical Technology and Engineering, Fujian Medical University, Fujian, China 350004. ; Zhiyuan Zheng:1.Department of Laboratory Medicine, The School of Medical Technology and Engineering, Fujian Medical University, Fujian, China 350004. ; Xiandong Jiang:1.Department of Laboratory Medicine, The School of Medical Technology and Engineering, Fujian Medical University, Fujian, China 350004. ; Yan Xue:1.Medical Technology Experimental Teaching Center, The School of Medical Technology
期刊:TXNRD1: A Key Regulator Involved in the Ferroptosis of CML Cells Induced by Cysteine Depletion In Vitro
影响因子 :0.0
引用产品: K-562 [K562] 细胞 , K-562 [K562] 细胞
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All-optical targeted drug delivery and real-time detection of a single cancer cell (2003-00-20)
作者:Xiaole Liu: 1.School of Materials Science and Engineering, State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-Sen University, Guangzhou 510275, China; Jie Yuan: 1.School of Medical Informatics, Daqing Campus, Harbin Medical University, Daqing163319, China; Dong Wu: 1.School of Materials Science and Engineering, State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-Sen University, Guangzhou 510275, China; Xiaobin Zou: 1.School of Materials Science and Engineering, State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-Sen University, Guangzhou 510275, China; Qing Zheng: 1.School of Materials Science and Engineering, State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-Sen University, Guangzhou 510275, China; Weina Zhang: 1.School of Materials Science and Engineering, State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-Sen University, Guangzhou 510275, China; Hongxiang Lei: 1.Sch
期刊:All-optical targeted drug delivery and real-time detection of a single cancer cell
影响因子 :7.5
引用产品: K-562 [K562] 细胞
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Rational Engineering a Multichannel Upconversion Sensorfor Multiplex Detection of Matrix Metalloproteinases Activities (2007-08-20)
作者:Sunan Cao:1.The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science , Soochow University , Suzhou , 215123 , P. R. China. ; Zhi Li:1.The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science , Soochow University , Suzhou , 215123 , P. R. China. ; Jinyuan Zhao:1.The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science , Soochow University , Suzhou , 215123 , P. R. China. ; Mi Chen:1.The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science , Soochow University , Suzhou , 215123 , P. R. China. ; Nan Ma:1.The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science , Soochow University , Suzhou , 215123 , P. R. China. ;
期刊:Rational Engineering a Multichannel Upconversion Sensorfor Multiplex Detection of Matrix Metalloproteinases Activities
DOI:10.1021/acssensors.8b00320
影响因子 :8.9
引用产品: HT-1080 [HT1080] 细胞 , K-562 [K562] 细胞
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