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产品概述
名称 | K-562 [K562] (人慢性髓原白血病细胞) (STR鉴定正确) |
别称 | K562; K 562; GM05372 |
种属 | 人 |
生长特性 | 悬浮细胞 |
细胞形态 | 淋巴细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 3×10^5-5×10^5cells/mL |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。 |
背景描述 | 连续细胞株K-562是由Lozzio从一位临终的53岁女性慢性骨髓性白血病患者的胸水中建立。K-562细胞被归入高度未分化的粒性白细胞类,Erson等对其表面膜性质的研究表明,K-562细胞是一株人类红白血病细胞。K-562细胞在自然杀伤分析中广泛作为高敏感的体外受体,See Pross等将K-562细胞用于NK细胞的体外详细检测,包括NK细胞活性的数学量化。K-562母细胞是多能性造血恶性细胞,它能自发分化成可识别的红血球祖细胞、粒性白细胞、单核细胞等。K-562细胞是EBNA阴性的。 |
年龄(性别) | 女性;53岁 |
组织来源 | 骨髓;慢性骨髓性白血病(CML) |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 白血病细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~30-48 hours |
致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
保藏机构 | ATCC; CCL-243 BCRC; 60007 BCRJ; 0126 CCLV; CCLV-RIE 0439 DSMZ; ACC-10 ECACC; 89121407 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 9,10 D2S1338 17 D3S1358 16 D5S818 11,12 D7S820 9,11 D8S1179 12 D13S317 8 D16S539 11,12 D18S51 15,16 D19S433 14,14.2 D21S11 29,31 FGA 21,24 PentaD 9,13 PentaE 5,14 TH01 9.3 TPOX 8,9 vWA 16 D6S1043 11 D12S391 23 D2S441 10,14 -
STR鉴定图
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参考文献
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A Divalent Chikungunya and Zika Nanovaccine with Thermostable Self-assembly Multivalent Scaffold LS-SUMO (2024-02-10)
期刊:Advanced Healthcare Materials
影响因子 :10.0
引用产品: K-562 [K562] 细胞 , IMDM 培养基
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Selection of reference genes in liproxstatin-1-treated K562 Leukemia cells via RT-qPCR and RNA sequencing (2024-01-02)
期刊:MOLECULAR BIOLOGY REPORTS
DOI:10.1007/s11033-023-08912-5
影响因子 :2.8
引用产品: K-562 [K562] 细胞
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Label-free, rapid and highly accurate identification and categorization of leukemia cells via Raman spectroscopy (2023-08-22)
期刊:SENSORS AND ACTUATORS B-CHEMICAL
影响因子 :8.4
引用产品: K-562 [K562] 细胞 , U-937 细胞 , Raji 细胞 , Jurkat, Clone E6-1 细胞 , K-562 [K562]细胞专用培养基 , U-937细胞专用培养基 , Raji细胞专用培养基 , Jurkat, Clone E6-1细胞专用培养基
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Development and evaluation of novel artemisinin-isatin hybrids with potential anti-leukemic cytotoxicity (2023-04-14)
期刊:Frontiers in Oncology
影响因子 :4.7
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Ester tethered artemisinin-isatin hybrids: design, synthesis and anti-leukemic activity evaluation (2023-08-21)
期刊:MEDICINAL CHEMISTRY RESEARCH
DOI:10.1007/s00044-023-03122-x
影响因子 :2.6
引用产品: K-562 [K562] 细胞 , K562/ADR 细胞 , CCRF-CEM [CCRF CEM] 细胞 , 人外周血单核细胞
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Pentanoate-Tethered Artemisinin-Isatin Hybrids with Antileukemic Potential (2023-03-21)
期刊:ChemistrySelect
影响因子 :2.1
引用产品: K-562 [K562] 细胞 , K562/ADR 细胞 , CCRF-CEM [CCRF CEM] 细胞
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Tet2-mediated DNA demethylation regulates the proliferation and apoptosis of human leukemia K562 cells (2023-03-31)
期刊:CELLULAR AND MOLECULAR BIOLOGY
影响因子 :1.6
引用产品: K-562 [K562] 细胞
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Liproxstatin‑1 induces cell cycle arrest, apoptosis, and caspase‑3/GSDME‑dependent secondary pyroptosis in K562 cells (2022-08-17)
期刊:INTERNATIONAL JOURNAL OF ONCOLOGY
影响因子 :5.9
引用产品: K-562 [K562] 细胞 , SH-SY5Y [SHSY-5Y] 细胞
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Lipid droplets as endogenous intracellular microlenses (2012-01-20)
期刊:Light-Science & Applications
DOI:10.1038/s41377-021-00687-3
影响因子 :19.4
引用产品: K-562 [K562] 细胞
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CXCR5 guides migration and tumor eradication of anti-EGFR chimeric antigen receptor T cells (2007-01-20)
期刊:Molecular Therapy-Oncolytics
DOI:10.1016/j.omto.2021.07.003
影响因子 :5.7
引用产品: A549/DDP 细胞 , COS-1 细胞 , K-562 [K562] 细胞 , NCI-H929 细胞
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Human m6A-mRNA and lncRNA epitranscriptomic microarray reveal function of RNA methylation in hemoglobin H-constant spring disease (2010-01-20)
期刊:Scientific Reports
DOI:10.1038/s41598-021-99867-9
影响因子 :4.6
引用产品: K-562 [K562] 细胞
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A novel upregulated LncRNA-AC026150.8 promotes chemo-resistance and predicts poor prognosis in acute myeloid leukemia (2010-01-20)
期刊:Cancer Medicine
影响因子 :4.0
引用产品: KG-1 细胞 , K-562 [K562] 细胞
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TXNRD1: A Key Regulator Involved in the Ferroptosis of CML Cells Induced by Cysteine Depletion In Vitro (2012-01-20)
期刊:Oxidative Medicine and Cellular Longevity
影响因子 :0.0
引用产品: K-562 [K562] 细胞 , K-562 [K562] 细胞
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All-optical targeted drug delivery and real-time detection of a single cancer cell (2003-00-20)
期刊:Nanophotonics
影响因子 :7.5
引用产品: K-562 [K562] 细胞
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Rational Engineering a Multichannel Upconversion Sensorfor Multiplex Detection of Matrix Metalloproteinases Activities (2007-08-20)
期刊:ACS Sensors
DOI:10.1021/acssensors.8b00320
影响因子 :8.9
引用产品: HT-1080 [HT1080] 细胞 , K-562 [K562] 细胞
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