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产品概述
名称 | HCC1954 (人乳腺导管癌细胞) (STR鉴定正确) |
别称 | HCC-1954; Hamon Cancer Center 1954 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样,有空泡 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:4-1:8 |
推荐换液频率 | 2-3次/周 |
背景描述 | HCC1954 is an epithelial cell line isolated from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases from a 61-year-old, Asian female in 1995. HCC1954 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR). Her2/neu is overexpressed in the ELISA assay. |
年龄(性别) | 女性;61岁 |
组织来源 | Breast; Duct; Mammary gland |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 乳腺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | 45 hours (PubMed=25984343); 43.81 hours (GrayJW panel) |
受体表达情况 | Estrogen receptor, not expressed; progesterone receptor, not expressed |
基因表达情况 | epithelial glycoprotein 2 (EGP2); cytokeratin 19 |
保藏机构 | ATCC; CRL-2338 BCRJ; 0280 KCLB; 9S1954 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10 D2S1338 17,26 D3S1358 15,16 D5S818 11 D7S820 10,11 D8S1179 12,15 D13S317 8,9 D16S539 9,11 D18S51 14,18 D19S433 13,15.2 D21S11 28,32.2 FGA 22,23 PentaD 9,12 PentaE 12,16 TH01 6,7 TPOX 8,9 vWA 18,19 D6S1043 11,14 D12S391 22 D2S441 10,11 -
STR鉴定图
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参考文献
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ERBB3-related gene PBX1 is associated with prognosis in patients with HER2-positive breast cancer (2025-01-10)
期刊:BMC Genomic Data
DOI:10.1186/s12863-024-01292-0
影响因子 :1.9
引用产品: MCF 10A 细胞 , HCC1954 细胞 , BT-474 [BT474] 细胞
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Tanshinone I improves TNBC chemosensitivity by suppressing late-phase autophagy through AKT/p38 MAPK signaling pathway (2024-07-02)
期刊:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2024.117037
影响因子 :6.9
引用产品: HCC1937 细胞 , HCC1954 细胞 , 重组人胰岛素溶液(10 mg/mL)
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