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产品概述
名称 | MDA-MB-453 (人乳腺癌细胞) (STR鉴定正确) |
别称 | MDA-MB 453; MDA MB 453; MDA-MB453; MDAMB453; MDA-453; MDA453; MD Anderson-Metastatic Breast-453 |
种属 | 人类 |
生长特性 | 半贴半悬 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,100%, 温度:37℃
|
培养方案B(可选) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 | 1.该细胞推荐使用Leibovitz's L-15培养基进行培养,Leibovitz's L-15不可以通入二氧化碳,会产生细胞毒性;2.如您没有无二氧化碳的培养箱,可使用DMEM替代Leibovitz's L-15,使用DMEM培养基时即可正常通入5%二氧化碳;3.配套专用培养基默认Leibovitz's L-15配置,如需DMEM配方,请联系销售下单备注更改。 |
背景描述 | MDA-MB-453细胞是由R·Cailleau等在1976年从一位48岁女性肿瘤转移患者胸水中建立的细胞株,其它转移灶包括淋巴结、脑和胸水及心包腔积水;MDA-MB-453细胞过表达FGF受体。 |
年龄(性别) | 女性,48岁 |
组织来源 | 乳腺;源自转移部位:胸腔积液 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 乳腺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~26-38 hours |
致瘤性 | No, in immunosuppressed mice.Yes, in semisolid medium. |
受体表达情况 | fibroblast growth factor (FGF), expressed |
保藏机构 | ATCC; HTB-131 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10,12 D2S1338 23,24 D3S1358 15 D5S818 11 D7S820 10 D8S1179 10,12 D13S317 12 D16S539 9 D18S51 15,20 D19S433 13,14 D21S11 29,31 FGA 18,23 PentaD 9,10 PentaE 11 TH01 6 TPOX 10 vWA 17,18 D6S1043 11,18 D12S391 18,21 D2S441 10 -
STR鉴定图
参考文献
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Identification of Piperazinyl–Difluoro-indene Derivatives Containing Pyridyl Groups as Potent FGFR Inhibitors against FGFR Mutant Tumor: Design, Synthesis, and Biological Evaluation (2024-01-31)
作者:Wei Ding:1.Beijing Key Laboratory of Active Substance Discovery and Druggability Evaluation, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, P. R. China. ; Liting Yan:1.College of Life Sciences, Shaanxi Normal University, Xi'an 710119, P. R. China. ; Li Sheng:1.Beijing Key Laboratory of Active Substance Discovery and Druggability Evaluation, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, P. R. China. ; Shuting Chen:1.College of Life Sciences, Shaanxi Normal University, Xi'an 710119, P. R. China. ; Ying Li:1.College of Life Sciences, Shaanxi Normal University, Xi'an 710119, P. R. China. ; Shihao Cheng:1.Beijing Key Laboratory of Active Substance Discovery and Druggability Evaluation, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, P. R. China. ; Lijun Luo:1.Beijing Key Laboratory of
期刊:Identification of Piperazinyl–Difluoro-indene Derivatives Containing Pyridyl Groups as Potent FGFR Inhibitors against FGFR Mutant Tumor: Design, Synthesis, and Biological Evaluation
DOI:10.1021/acs.jmedchem.3c02040
影响因子 :7.3
引用产品: KMS-11 细胞 , MDA-MB-453 细胞
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A novel feedback regulated loop of circRRM2-IGF2BP1-MYC promotes breast cancer metastasis (2023-03-25)
作者:Ran Hao:1.Institutes of Health Research, Hebei Medical University, Shijiazhuang, Hebei, China. ; Lei Zhang:1.Institutes of Health Research, Hebei Medical University, Shijiazhuang, Hebei, China. ; Yangming Si:1.School of Physical Science and Technology, Inner Mongolia University, Hohhot, Inner Mongolia, China. ; Peng Zhang:1.Institutes of Health Research, Hebei Medical University, Shijiazhuang, Hebei, China. ; 2.Department of Military Nursing, NCO School, Army Medical University, Shijiazhuang, Hebei, China. ; Yipeng Wang:1.Institutes of Health Research, Hebei Medical University, Shijiazhuang, Hebei, China. ; Bangchao Li:1.Department of Breast Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China. ; Jie Hu:1.Department of Science and Technology, Hebei Medical University, Shijiazhuang, Hebei, China. Hujie@hebmu.edu.cn. ; Yixin Qi:1.Department of Breast Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China. qiyixin@hebmu.ed
期刊:A novel feedback regulated loop of circRRM2-IGF2BP1-MYC promotes breast cancer metastasis
DOI:10.1186/s12935-023-02895-w
影响因子 :5.8
引用产品: MCF 10A 细胞 , BT-474 [BT474] 细胞 , BT-549 细胞 , MCF7 [MCF-7] 细胞 , MDA-MB-231 细胞 , MDA-MB-231 细胞 , MDA-MB-453 细胞 , MDA-MB-453 细胞 , T-47D 细胞 , MCF 10A细胞专用培养基 , BT-474 [BT474]细胞专用培养基 , BT-549细胞专用培养基 , MCF7 [MCF-7]细胞专用培养基 , MDA-MB-231细胞专用培养基 (L15) , MDA-MB-231细胞专用培养基 (DMEM) , MDA-MB-453细胞专用培养基 (L15)
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A Novel Optical Fiber Terahertz Biosensor Based on Anti-Resonance for The Rapid and Nondestructive Detection of Tumor Cells (2023-10-23)
作者:Zhe He:1.Department of Laboratory Medicine, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, China. ; Yueping Luo:1.Tianjin Key Laboratory of Optoelectronic Detection Technology and System, School of Electronic and Information Engineering, Tiangong University, Tianjin 300387, China. ; Guorong Huang:1.Department of Laboratory Medicine, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, China. ; Marc Lamy de la Chapelle:1.Department of Laboratory Medicine, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, China. ; 2.Institut des Molécules et Matériaux du Mans (IMMM-UMR CNRS 6283), Université du Mans, Avenue Olivier Messiaen, 72085 Le Mans, France. ; Huiyan Tian:1.Department of Laboratory Medicine, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, China. ; Fengxin Xie:1.Department of Laborat
期刊:A Novel Optical Fiber Terahertz Biosensor Based on Anti-Resonance for The Rapid and Nondestructive Detection of Tumor Cells
影响因子 :5.4
引用产品: MDA-MB-453 细胞 , MDA-MB-231 细胞 , BT-474 [BT474] 细胞
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Potentiated effects of lactate receptor GPR81 on immune microenvironment in breast cancer (2023-05-30)
作者:Shenchao Guo:1.Department of Radiotherapy and Chemotherapy, The First Affiliated Hospital of Ningbo University, Ningbo, People's Republic of China. ; 2.Department of Thyroid and Breast Surgery, The First Affiliated Hospital of Ningbo University, Ningbo, People's Republic of China. ; Jianliang Zhou:1.Department of Radiotherapy and Chemotherapy, The First Affiliated Hospital of Ningbo University, Ningbo, People's Republic of China. ; Pengrong Lou:1.Department of Radiotherapy and Chemotherapy, The First Affiliated Hospital of Ningbo University, Ningbo, People's Republic of China. ; Lijuan Weng:1.Department of Radiotherapy and Chemotherapy, The First Affiliated Hospital of Ningbo University, Ningbo, People's Republic of China. ; Xiaoxian Ye:1.Department of Radiotherapy and Chemotherapy, The First Affiliated Hospital of Ningbo University, Ningbo, People's Republic of China. ; Jianxin Guo:1.Department of Radiotherapy and Chemotherapy, The First Affiliated Hospital of Ningbo University,
期刊:Potentiated effects of lactate receptor GPR81 on immune microenvironment in breast cancer
DOI:10.1002/mc.23582
影响因子 :4.6
引用产品: BT-549 细胞 , MDA-MB-453 细胞 , THP-1 细胞
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Isopimaric acid, an ion channel regulator, regulates calcium and oxidative phosphorylation pathways to inhibit breast cancer proliferation and metastasis (2023-02-06)
作者:Jiacheng Li:1.College of Life Science, Sichuan Normal University, Chengdu, Sichuan 610101, China; Zhejiang Provincial Key Laboratory of Cancer Prevention and Treatment Technology of Integrated Traditional Chinese and Western Medicine, Zhejiang Academy of Traditional Chinese Medicine, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, 310012, China. ; Xiaozhen Liu:1.Department of Medical and Radiation Oncology, Linyi People's Hospital, Linyi 276000, China. ; Lin Chen:1.Sericultural Research Institute, Zhejiang, Academy of Agricultural Sciences, Hangzhou 310021, China. ; Xinping Zhu:1.Zhejiang Provincial Key Laboratory of Cancer Prevention and Treatment Technology of Integrated Traditional Chinese and Western Medicine, Zhejiang Academy of Traditional Chinese Medicine, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, 310012, China. ; Zhihong Yu:1.Zhejiang Provincial Key Laboratory of Cancer Prevention and Treatment Technology of Integrated Traditional Chinese and Weste
期刊:Isopimaric acid, an ion channel regulator, regulates calcium and oxidative phosphorylation pathways to inhibit breast cancer proliferation and metastasis
DOI:10.1016/j.taap.2023.116415
影响因子 :3.8
引用产品: MDA-MB-231 细胞 , MCF7 [MCF-7] 细胞 , MDA-MB-468 细胞 , HCC1937 细胞 , MDA-MB-453 细胞 , 4T1 细胞 , MCF 10A 细胞 , Annexin V-FITC/ PI荧光双染细胞凋亡检测试剂盒
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Identification of TUBB2A as a Cancer-Immunity Cycle-Related Therapeutic Target in Triple-Negative Breast Cancer (2023-09-24)
作者:Jia Li:1.Department of Breast Surgical Oncology, Shanxi Province Cancer Hospital/Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Xinghualing District, Taiyuan, 030013, Shanxi Province, People's Republic of China. ; Jingchun Yao:1.Department of Head and Neck, Shanxi Province Cancer Hospital/Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Xinghualing District, Taiyuan, 030013, Shanxi Province, People's Republic of China. ; Liqiang Qi:1.Department of Breast Surgical Oncology, Cancer Institute and Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No.17 Panjiayuan, Huawei South Road, Chaoyang District, Beijing, 100021, People's Republic of China. qi_liqiang@outlook.com. ;
期刊:Identification of TUBB2A as a Cancer-Immunity Cycle-Related Therapeutic Target in Triple-Negative Breast Cancer
DOI:10.1007/s12033-023-00880-2
影响因子 :2.6
引用产品: MCF 10A 细胞 , MDA-MB-231 细胞 , MDA-MB-453 细胞 , DMEM高糖 培养基 , 特级胎牛血清
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Asiaticoside hampers epithelial–mesenchymal transition by promoting PPARG expression and suppressing P2RX7-mediated TGF-β/Smad signaling in triple-negative breast cancer (2022-11-28)
作者:Xuemei Huang:1.Department of Oncology and Hematology, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, China. ; Zhiqin Jia:1.Department of Obstetrics and Gynecology, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, China. ; Xiangyue Li:1.Department of Obstetrics and Gynecology, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, China. ; Zhilan Hu:1.Department of Obstetrics and Gynecology, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, China. ; Xiaolan Yu:1.Department of Obstetrics and Gynecology, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, China. ; Jiyi Xia:1.Department of Oncology and Hematology, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, China. ; 2.Dazhou Vocational College of Chinese Medicine, Dazhou,
期刊:Asiaticoside hampers epithelial–mesenchymal transition by promoting PPARG expression and suppressing P2RX7-mediated TGF-β/Smad signaling in triple-negative breast cancer
DOI:10.1002/ptr.7692
影响因子 :6.4
引用产品: 人乳腺上皮细胞 , MDA-MB-453 细胞 , MDA-MB-231细胞专用培养基 (DMEM) , MCF7 [MCF-7] 细胞 , ZR-75-1 细胞
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RAMP2-AS1 inhibits CXCL11 expression to suppress malignant phenotype of breast cancer by recruiting DNMT1 and DNMT3B (2004-02-20)
作者:Li Li:1.Department of Breast Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, PR China. Electronic address: 156333039@qq.com. ; Ya-Ping Gan:1.Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, Guangdong Province, PR China. ; Hui Peng:1.Nanchang University, Nanchang 330006, Jiangxi Province, PR China. ;
期刊:RAMP2-AS1 inhibits CXCL11 expression to suppress malignant phenotype of breast cancer by recruiting DNMT1 and DNMT3B
DOI:10.1016/j.yexcr.2022.113139
影响因子 :3.7
引用产品: HCC1937 细胞 , MDA-MB-231 细胞 , MDA-MB-453 细胞 , MCF7 [MCF-7] 细胞 , MCF 10A 细胞
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Up-regulated circBACH2 contributes to cell proliferation, invasion, and migration of triple-negative breast cancer (2004-01-20)
作者:Xinxing Wang:1.Department of Breast Surgery, the First Affiliated Hospital of Zhengzhou University, No.1 Jianshedong Road, Erqi District, Zhengzhou, Henan Province, People's Republic of China. ; Bingjian Xue:1.Department of Breast Surgery, the First Affiliated Hospital of Zhengzhou University, No.1 Jianshedong Road, Erqi District, Zhengzhou, Henan Province, People's Republic of China. ; Yujie Zhang:1.Department of Breast Surgery, the First Affiliated Hospital of Zhengzhou University, No.1 Jianshedong Road, Erqi District, Zhengzhou, Henan Province, People's Republic of China. ; Guangcheng Guo:1.Department of Breast Surgery, the First Affiliated Hospital of Zhengzhou University, No.1 Jianshedong Road, Erqi District, Zhengzhou, Henan Province, People's Republic of China. ; Xin Duan:1.Department of Breast Surgery, the First Affiliated Hospital of Zhengzhou University, No.1 Jianshedong Road, Erqi District, Zhengzhou, Henan Province, People's Republic of China. ; Dongwei Dou:1.Departmen
期刊:Up-regulated circBACH2 contributes to cell proliferation, invasion, and migration of triple-negative breast cancer
DOI:10.1038/s41419-021-03684-x
影响因子 :9.0
引用产品: MDA-MB-231 细胞 , MDA-MB-468 细胞 , MDA-MB-453 细胞 , BT-549 细胞 , MCF 10A 细胞 , MCF7 [MCF-7] 细胞 , BT-474 [BT474] 细胞
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CircFBXL5 promotes the 5-FU resistance of breast cancer via modulating miR-216b/HMGA2 axis (2007-01-20)
作者:Mingzhi Zhu:1.Department of Breast Surgery, The First Affiliated Hospital of Zhengzhou University, Jianshe Dong Lu, Erqi District , Zhengzhou City, 450052 , Henan Province , China. ; Yanyan Wang:1.Department of Breast Surgery, The First Affiliated Hospital of Zhengzhou University, Jianshe Dong Lu, Erqi District , Zhengzhou City, 450052 , Henan Province , China. ; Fang Wang:1.Department of Breast Surgery, The First Affiliated Hospital of Zhengzhou University, Jianshe Dong Lu, Erqi District , Zhengzhou City, 450052 , Henan Province , China. ; Lin Li:1.Department of Breast Surgery, The First Affiliated Hospital of Zhengzhou University, Jianshe Dong Lu, Erqi District , Zhengzhou City, 450052 , Henan Province , China. ; Xinguang Qiu:1.Department of Thyroid Surgery, The First Affiliated Hospital of Zhengzhou University, Jianshe Dong Lu, Erqi District, Zhengzhou City, 450052, Henan Province, China. qxg6309@163.com. ;
期刊:CircFBXL5 promotes the 5-FU resistance of breast cancer via modulating miR-216b/HMGA2 axis
DOI:10.1186/s12935-021-02088-3
影响因子 :5.8
引用产品: MCF 10A 细胞 , MDA-MB-453 细胞 , MDA-MB-231 细胞 , DMEM/F12 培养基
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Circular RNA circ-ERBB2 Elevates the Warburg Effect and Facilitates Triple-Negative Breast Cancer Growth by the MicroRNA 136-5p/Pyruvate Dehydrogenase Kinase 4 Axis | Molecular and Cellular Biology (2009-01-20)
作者:Yihong Huang:1.Department of Ultrasound Diagnosis, Fuzhou Second Hospital affiliated with Xiamen University, Fuzhou, Fujian, China. ; Shuo Zheng:1.Department of Hepatobiliary Surgery, Fuzhou Second Hospital affiliated with Xiamen University, Fuzhou, Fujian, China. ; Ying Lin:1.Department of Ultrasound Diagnosis, Fuzhou Second Hospital affiliated with Xiamen University, Fuzhou, Fujian, China. ; Liming Ke:1.Department of Ultrasound Diagnosis, Fuzhou Second Hospital affiliated with Xiamen University, Fuzhou, Fujian, China. ;
期刊:Circular RNA circ-ERBB2 Elevates the Warburg Effect and Facilitates Triple-Negative Breast Cancer Growth by the MicroRNA 136-5p/Pyruvate Dehydrogenase Kinase 4 Axis | Molecular and Cellular Biology
影响因子 :5.3
引用产品: BT-549 细胞 , MDA-MB-453 细胞 , MDA-MB-468 细胞 , MDA-MB-231 细胞 , MCF 10A 细胞 , RPMI-1640 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , Leibovitz's L-15 培养基
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circKLHL24 Blocks Breast Cancer Development by Regulating the miR-1204/ALX4 Network (2003-01-20)
作者:Guangming Yi:1.Department of Oncology, The Third Hospital of Mianyang (Sichuan Mental Health Center), Mianyang City, China. ; Dan Wang:1.Department of Radiology, Mianyang Central Hospital, Mianyang City, China. ; Jianjun Han:1.Department of Oncology, The Third Hospital of Mianyang (Sichuan Mental Health Center), Mianyang City, China. ; Li Jia:1.Department of Oncology, The Third Hospital of Mianyang (Sichuan Mental Health Center), Mianyang City, China. ; Xiaojun Liu:1.Department of Oncology, The Third Hospital of Mianyang (Sichuan Mental Health Center), Mianyang City, China. ; Jun He:1.Department of Oncology, The Third Hospital of Mianyang (Sichuan Mental Health Center), Mianyang City, China. ;
期刊:circKLHL24 Blocks Breast Cancer Development by Regulating the miR-1204/ALX4 Network
影响因子 :3.6
引用产品: BT-549 细胞 , MDA-MB-231 细胞 , HCC38 细胞 , SUM159PT 细胞 , MDA-MB-453 细胞 , MCF 10A 细胞
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Sericin inhibits MDA‑MB‑468 cell proliferation via the PI3K/Akt pathway in triple‑negative breast cancer (2012-00-20)
作者:Lin Niu:1.Department of Human Anatomy, Chengde Medical University, Chengde, Hebei 067000, P.R. China. ; Songhe Yang:1.Department of Human Anatomy, Chengde Medical University, Chengde, Hebei 067000, P.R. China. ; Xueying Zhao:1.Department of Immunology, Chengde Medical University, Chengde, Hebei 067000, P.R. China. ; Xiaochao Liu:1.Department of Human Anatomy, Chengde Medical University, Chengde, Hebei 067000, P.R. China. ; Lina Si:1.Department of Human Anatomy, Chengde Medical University, Chengde, Hebei 067000, P.R. China. ; Meng Wei:1.Department of Human Anatomy, Chengde Medical University, Chengde, Hebei 067000, P.R. China. ; Lei Liu:1.Department of Immunology, Chengde Medical University, Chengde, Hebei 067000, P.R. China. ; Luyang Cheng:1.Department of Immunology, Chengde Medical University, Chengde, Hebei 067000, P.R. China. ; Yuebing Qiao:1.Department of Human Anatomy, Chengde Medical University, Chengde, Hebei 067000, P.R. China. ; Zhihong Chen:1.Department of Human Anat
期刊:Sericin inhibits MDA‑MB‑468 cell proliferation via the PI3K/Akt pathway in triple‑negative breast cancer
影响因子 :3.4
引用产品: MDA-MB-468 细胞 , MDA-MB-453 细胞 , MCF7 [MCF-7] 细胞 , MCF 10A 细胞
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miR-29b-3p promotes progression of MDA-MB-231 triple-negative breast cancer cells through downregulating TRAF3 (2007-09-20)
作者:Bao Zhang:1.School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510640, People's Republic of China. ; Dattatrya Shetti:1.School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510640, People's Republic of China. ; Conghui Fan:1.School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510640, People's Republic of China. ; Kun Wei:1.School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510640, People's Republic of China. weikun@scut.edu.cn. ;
期刊:miR-29b-3p promotes progression of MDA-MB-231 triple-negative breast cancer cells through downregulating TRAF3
影响因子 :6.7
引用产品: MDA-MB-231 细胞 , MCF7 [MCF-7] 细胞 , MDA-MB-453 细胞 , MCF 10A 细胞 , MCF 10A细胞专用培养基
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