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BT-474 [BT474] (人乳腺导管癌细胞) (STR鉴定正确)
货号:CL-0040
规格:1×10⁶cells/T25培养瓶
价格: ¥1600
生长培养基:RPMI-1640+10μg/ml Insulin+2mM L-glutamine+20% FBS+1% P/S
产品概述
名称 | BT-474 [BT474] (人乳腺导管癌细胞) (STR鉴定正确) |
别称 | Bt-474; BT474 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 | 1.BT-474细胞生长缓慢,细胞主要是成片生长;2.汇合至80%即可传代,细胞不会长太满,5-6天传代一次。 |
背景描述 | BT-474 [BT474]细胞是由E·Lasfargues和W·G·Coutinho从一个实心的乳腺浸润性导管癌中分离到的细胞株。 |
年龄(性别) | 女性;60岁 |
组织来源 | 乳腺导管癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 乳腺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~84-100 hours |
致瘤性 | Yes, in Amsterdam/IMR rats with regression in 10 days.Yes, in nude mice. |
保藏机构 | ATCC; CRL-7913ATCC; HTB-20 BCRC; 60359 BCRJ; 0353 DSMZ; ACC-64 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10,11 D2S1338 19 D3S1358 17 D5S818 11,13 D7S820 9,12 D8S1179 10,12 D13S317 11 D16S539 9,11 D18S51 13,18 D19S433 14,17 D21S11 28,32.2 FGA 22,25 PentaD 9,14 PentaE 5 TH01 7 TPOX 8 vWA 15,16 D6S1043 12,17 D12S391 17,18 D2S441 10,14 -
STR鉴定图
参考文献
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Long-term cadmium exposure induces epithelial-mesenchymal transition in breast cancer cells by activating CYP1B1-mediated glutamine metabolic reprogramming in BT474 cells and MMTV-Erbb2 mice (2024-02-07)
作者:Jingdian Li:1.Department of Occupational Health (Key Laboratory of Electromagnetic Radiation Protection, Ministry of Education), Army Medical University (Third Military Medical University), Chongqing 400038, China. ; Peng Gao:1.Department of Occupational Health (Key Laboratory of Electromagnetic Radiation Protection, Ministry of Education), Army Medical University (Third Military Medical University), Chongqing 400038, China. ; Mingke Qin:1.Department of Occupational Health (Key Laboratory of Electromagnetic Radiation Protection, Ministry of Education), Army Medical University (Third Military Medical University), Chongqing 400038, China. ; Junhua Wang:1.Nuclear Medicine Department, General Hospital of Tibet Military Area Command, Lhasa 850000, Xizang, China. ; Yan Luo:1.Department of Occupational Health (Key Laboratory of Electromagnetic Radiation Protection, Ministry of Education), Army Medical University (Third Military Medical University), Chongqing 400038, China. ; Ping Deng:1.
期刊:Long-term cadmium exposure induces epithelial-mesenchymal transition in breast cancer cells by activating CYP1B1-mediated glutamine metabolic reprogramming in BT474 cells and MMTV-Erbb2 mice
DOI:10.1016/j.scitotenv.2024.170773
影响因子 :9.8
引用产品: BT-474 [BT474] 细胞
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UCHL1 contributes to insensitivity to endocrine therapy in triple-negative breast cancer by deubiquitinating and stabilizing KLF5 (2024-03-11)
作者:Juan Li:1.Department of Breast Surgery, School of Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China. ; Yu Liang:1.Department of Health Management & Institute of Health Management, School of Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China. ; Shijie Zhou:1.State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, China. vicjaychou@hotmail.com. ; Jie Chen:1.Department of Breast Surgery, School of Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China. Chenjie20201019@163.com. ; Chihua Wu:1.Department of Breast Surgery, School of Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China. 18749019@qq.com. ;
期刊:UCHL1 contributes to insensitivity to endocrine therapy in triple-negative breast cancer by deubiquitinating and stabilizing KLF5
DOI:10.1186/s13058-024-01800-1
影响因子 :7.4
引用产品: MCF7 [MCF-7] 细胞 , BT-474 [BT474] 细胞 , SUM149PT 细胞 , MDA-MB-468 细胞
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Analysis of TLR2 in Primary Endocrine Resistant of Breast Cancer (2024-02-22)
作者:Yunmei Wang:1.Department of Medical Oncology, First Affiliated Hospital of Xi'an Jiao Tong University, 710061 Xi'an, Shaanxi, China. ; 2.Department of Medical Oncology, Shaanxi Provincial Cancer Hospital Affiliated to Medical School, Xi'an Jiao Tong University, 710061 Xi'an, Shaanxi, China. ; Guangxi Wang:1.Department of Medical Oncology, Shaanxi Provincial Cancer Hospital Affiliated to Medical School, Xi'an Jiao Tong University, 710061 Xi'an, Shaanxi, China. ; Xiang Wang:1.Department of Medical Oncology, Shaanxi Provincial Cancer Hospital Affiliated to Medical School, Xi'an Jiao Tong University, 710061 Xi'an, Shaanxi, China. ; Jiao Yang:1.Department of Medical Oncology, First Affiliated Hospital of Xi'an Jiao Tong University, 710061 Xi'an, Shaanxi, China. ; Yanwei Shen:1.Department of Breast Surgery, Shaanxi Provincial People's Hospital, 710061 Xi'an, Shaanxi, China. ; Bin Zhao:1.Department of Epidemiology, Shaanxi Provincial Cancer Hospital Affiliated to Medical School, Xi'an Ji
期刊:Analysis of TLR2 in Primary Endocrine Resistant of Breast Cancer
影响因子 :3.1
引用产品: BT-474 [BT474] 细胞 , MCF7 [MCF-7] 细胞 , MCF 10A 细胞 , STR鉴定
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Development of a rare earth nanoprobe enables in vivo real-time detection of sentinel lymph node metastasis of breast cancer using NIR-IIb imaging (2023-08-04)
作者:Yuan-Yuan Zhu:1.Department of Breast-Thyroid-Surgery and Cancer Center, Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China. ; 2.Fujian Key Laboratory of Precision Diagnosis and Treatment in Breast Cancer (Xiang'an Hospital of Xiamen University), Xiamen, China. ; 3.Xiamen Key Laboratory for Endocrine Related Cancer Precision Medicine, Xiang'an Hospital of Xiamen University, Xiamen, China. ; 4.Xiamen Research Center of Clinical Medicine in Breast & Thyroid Cancers, Xiamen, China. ; Liang Song:1.State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, China. ; 2.Xiamen Key Laboratory of Rare Earth Photoelectric Functional Materials, Xiamen Institute of Rare Earth Materials, Haixi Institute, Chinese Academy of Sciences, Xiamen, China. ; Yong-Qu Zhang:1.Department of Breast-Thyroid-Surgery and Cancer Center, Xiang'an Hospital of Xiamen University, School of Medicine
期刊:Development of a rare earth nanoprobe enables in vivo real-time detection of sentinel lymph node metastasis of breast cancer using NIR-IIb imaging
DOI:10.1158/0008-5472.CAN-22-3432
影响因子 :11.2
引用产品: 4T1 细胞 , MDA-MB-231 细胞 , MCF7 [MCF-7] 细胞 , BT-549 细胞 , T-47D 细胞 , SK-BR-3 [SKBR3] 细胞 , BT-474 [BT474] 细胞 , MCF 10A 细胞
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CircEGFR reduces the sensitivity of pirarubicin and regulates the malignant progression of triple-negative breast cancer via the miR-1299/EGFR axis (2023-06-09)
作者:Jiulong Ma:1.Department of Experimental Pharmacology and Toxicology, School of Pharmaceutical Sciences, Jilin University, 1266 Fujin Road, Changchun, Jilin 130021, China. ; Chen Chen:1.Department of Pharmacology, School of Pharmaceutical Sciences, Jilin University, 1266 Fujin Road, Changchun, Jilin 130021, China. ; Zhimin Fan:1.General Surgery Center, Department of Breast Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, China. ; Yang Zhang:1.Department of Rehabilitation Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. ; Jiahua Ji:1.Department of Experimental Pharmacology and Toxicology, School of Pharmaceutical Sciences, Jilin University, 1266 Fujin Road, Changchun, Jilin 130021, China. ; Dexian Wei:1.Department of Experimental Pharmacology and Toxicology, School of Pharmaceutical Sciences, Jilin University, 1266 Fujin Road, Changchun, Jilin 130021, China. ; Fan Zhang:1.General Surgery Center, Department of Breast Surgery, The Firs
期刊:CircEGFR reduces the sensitivity of pirarubicin and regulates the malignant progression of triple-negative breast cancer via the miR-1299/EGFR axis
DOI:10.1016/j.ijbiomac.2023.125295
影响因子 :8.2
引用产品: MCF 10A 细胞 , BT-474 [BT474] 细胞 , SK-BR-3 [SKBR3] 细胞 , MDA-MB-231 细胞 , MCF 10A细胞专用培养基 , BT-474 [BT474]细胞专用培养基 , SK-BR-3 [SKBR3]细胞专用培养基 , MDA-MB-231细胞专用培养基 (L15)
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A novel feedback regulated loop of circRRM2-IGF2BP1-MYC promotes breast cancer metastasis (2023-03-25)
作者:Ran Hao:1.Institutes of Health Research, Hebei Medical University, Shijiazhuang, Hebei, China. ; Lei Zhang:1.Institutes of Health Research, Hebei Medical University, Shijiazhuang, Hebei, China. ; Yangming Si:1.School of Physical Science and Technology, Inner Mongolia University, Hohhot, Inner Mongolia, China. ; Peng Zhang:1.Institutes of Health Research, Hebei Medical University, Shijiazhuang, Hebei, China. ; 2.Department of Military Nursing, NCO School, Army Medical University, Shijiazhuang, Hebei, China. ; Yipeng Wang:1.Institutes of Health Research, Hebei Medical University, Shijiazhuang, Hebei, China. ; Bangchao Li:1.Department of Breast Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China. ; Jie Hu:1.Department of Science and Technology, Hebei Medical University, Shijiazhuang, Hebei, China. Hujie@hebmu.edu.cn. ; Yixin Qi:1.Department of Breast Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China. qiyixin@hebmu.ed
期刊:A novel feedback regulated loop of circRRM2-IGF2BP1-MYC promotes breast cancer metastasis
DOI:10.1186/s12935-023-02895-w
影响因子 :5.8
引用产品: MCF 10A 细胞 , BT-474 [BT474] 细胞 , BT-549 细胞 , MCF7 [MCF-7] 细胞 , MDA-MB-231 细胞 , MDA-MB-231 细胞 , MDA-MB-453 细胞 , MDA-MB-453 细胞 , T-47D 细胞 , MCF 10A细胞专用培养基 , BT-474 [BT474]细胞专用培养基 , BT-549细胞专用培养基 , MCF7 [MCF-7]细胞专用培养基 , MDA-MB-231细胞专用培养基 (L15) , MDA-MB-231细胞专用培养基 (DMEM) , MDA-MB-453细胞专用培养基 (L15)
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A Novel Optical Fiber Terahertz Biosensor Based on Anti-Resonance for The Rapid and Nondestructive Detection of Tumor Cells (2023-10-23)
作者:Zhe He:1.Department of Laboratory Medicine, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, China. ; Yueping Luo:1.Tianjin Key Laboratory of Optoelectronic Detection Technology and System, School of Electronic and Information Engineering, Tiangong University, Tianjin 300387, China. ; Guorong Huang:1.Department of Laboratory Medicine, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, China. ; Marc Lamy de la Chapelle:1.Department of Laboratory Medicine, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, China. ; 2.Institut des Molécules et Matériaux du Mans (IMMM-UMR CNRS 6283), Université du Mans, Avenue Olivier Messiaen, 72085 Le Mans, France. ; Huiyan Tian:1.Department of Laboratory Medicine, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, China. ; Fengxin Xie:1.Department of Laborat
期刊:A Novel Optical Fiber Terahertz Biosensor Based on Anti-Resonance for The Rapid and Nondestructive Detection of Tumor Cells
影响因子 :5.4
引用产品: MDA-MB-453 细胞 , MDA-MB-231 细胞 , BT-474 [BT474] 细胞
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Upregulation of the ferroptosis-related STEAP3 gene is a specific predictor of poor triple-negative breast cancer patient outcomes (2023-03-31)
作者:Lifang Yuan:1.Department of Oncology, Yantai Yuhuangding Hospital, Shandong University, Yantai, China. ; 2.Department of Breast Oncology, Huanxing Cancer Hospital, Beijing, China. ; Jiannan Liu:1.Department of Oncology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China. ; Lei Bao:1.Department of Pathology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China. ; Huajun Qu:1.Department of Oncology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China. ; Jinyu Xiang:1.Department of Oncology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China. ; Ping Sun:1.Department of Oncology, Yantai Yuhuangding Hospital, Shandong University, Yantai, China. ;
期刊:Upregulation of the ferroptosis-related STEAP3 gene is a specific predictor of poor triple-negative breast cancer patient outcomes
影响因子 :4.7
引用产品: MCF 10A 细胞 , MDA-MB-231 细胞 , BT-549 细胞 , MDA-MB-468 细胞 , MCF7 [MCF-7] 细胞 , T-47D 细胞 , BT-474 [BT474] 细胞
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A comprehensive analysis of the role of QPRT in breast cancer (2023-09-18)
作者:Yiqing Yan:1.Department of General Surgery, The Second Xiangya Hospital, Central South University, No. 139, Renmin Central Road, Changsha, 410011, China. ; Lun Li:1.Department of General Surgery, The Second Xiangya Hospital, Central South University, No. 139, Renmin Central Road, Changsha, 410011, China. ; Zixin Wang:1.Department of General Surgery, The Second Xiangya Hospital, Central South University, No. 139, Renmin Central Road, Changsha, 410011, China. ; Jian Pang:1.Department of General Surgery, The Second Xiangya Hospital, Central South University, No. 139, Renmin Central Road, Changsha, 410011, China. ; Xinyu Guan:1.Department of General Surgery, The Second Xiangya Hospital, Central South University, No. 139, Renmin Central Road, Changsha, 410011, China. ; Yunchang Yuan:1.Department of Thoracic Surgery, The Second Xiangya Hospital, Central South University, No. 139, Renmin Central Road, Changsha, 410011, China. ; Zhenkun Xia:1.Department of Thoracic Surgery, The Second Xi
期刊:A comprehensive analysis of the role of QPRT in breast cancer
DOI:10.1038/s41598-023-42566-4
影响因子 :4.6
引用产品: BT-474 [BT474] 细胞 , SK-BR-3 [SKBR3] 细胞 , THP-1 细胞
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TTK inhibition activates STING signal and promotes anti-PD1 immunotherapy in breast cancer (2023-12-18)
作者:Xiang Hu:1.Department of Emergency Medicine and Laboratory of Emergency Medicine, State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. ; Guangmei Li:1.Department of Emergency Medicine and Laboratory of Emergency Medicine, State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. ; Sisi Li:1.Department of Breast Cancer, Chongqing University Cancer Hospital, Chongqing, 400030, China. ; Qiwei Wang:1.Yangzhou Center for Disease Control and Prevention, Yangzhou, 225007, Jiangsu, China. ; Yuerong Wang:1.Department of Oncology and Hematology, Western Theater Command Air Force Hospital of PLA, Chengdu, 610083, Sichuan, China. ; Peidong Zhang:1.Department of Emergency Medicine and Laboratory of Emergency Medicine, State Key Laboratory of Biotherapy and Collaborative Innovation Center
期刊:TTK inhibition activates STING signal and promotes anti-PD1 immunotherapy in breast cancer
DOI:10.1016/j.bbrc.2023.149388
影响因子 :3.1
引用产品: 4T1 细胞 , 293T [HEK-293T] 细胞 , MDA-MB-231 细胞 , MDA-MB-468 细胞 , BT-549 细胞 , BT-474 [BT474] 细胞 , MCF7 [MCF-7] 细胞
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Deciphering the map of METTL14-mediated lncRNA m6A modification at the transcriptome-wide level in breast cancer (2022-11-06)
作者:Dandan Yi:1.Department of General Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China. ; Fazhan Xu:1.Department of General Surgery, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, China. ; Ru Wang:1.Department of General Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China. ; Chaoyu Jiang:1.Department of General Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China. ; Jiabo Qin:1.Department of General Surgery, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, China. ; YiHsuan Lee:1.Department of General Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China. ; Xianbiao Shi:1.Department of General Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanji
期刊:Deciphering the map of METTL14-mediated lncRNA m6A modification at the transcriptome-wide level in breast cancer
影响因子 :3.1
引用产品: MCF 10A 细胞 , MDA-MB-468 细胞 , MCF7 [MCF-7] 细胞 , SK-BR-3 [SKBR3] 细胞 , MDA-MB-231 细胞 , BT-474 [BT474] 细胞
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Up-regulated circBACH2 contributes to cell proliferation, invasion, and migration of triple-negative breast cancer (2004-01-20)
作者:Xinxing Wang:1.Department of Breast Surgery, the First Affiliated Hospital of Zhengzhou University, No.1 Jianshedong Road, Erqi District, Zhengzhou, Henan Province, People's Republic of China. ; Bingjian Xue:1.Department of Breast Surgery, the First Affiliated Hospital of Zhengzhou University, No.1 Jianshedong Road, Erqi District, Zhengzhou, Henan Province, People's Republic of China. ; Yujie Zhang:1.Department of Breast Surgery, the First Affiliated Hospital of Zhengzhou University, No.1 Jianshedong Road, Erqi District, Zhengzhou, Henan Province, People's Republic of China. ; Guangcheng Guo:1.Department of Breast Surgery, the First Affiliated Hospital of Zhengzhou University, No.1 Jianshedong Road, Erqi District, Zhengzhou, Henan Province, People's Republic of China. ; Xin Duan:1.Department of Breast Surgery, the First Affiliated Hospital of Zhengzhou University, No.1 Jianshedong Road, Erqi District, Zhengzhou, Henan Province, People's Republic of China. ; Dongwei Dou:1.Departmen
期刊:Up-regulated circBACH2 contributes to cell proliferation, invasion, and migration of triple-negative breast cancer
DOI:10.1038/s41419-021-03684-x
影响因子 :9.0
引用产品: MDA-MB-231 细胞 , MDA-MB-468 细胞 , MDA-MB-453 细胞 , BT-549 细胞 , MCF 10A 细胞 , MCF7 [MCF-7] 细胞 , BT-474 [BT474] 细胞
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TILRR (FREM1 isoform 2) is a prognostic biomarker correlated with immune infiltration in breast cancer (2010-00-20)
作者:Xiao-Yi Xu:1.CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, and Guangzhou Medical University, Guangzhou, Guangdong, China. ; 2.State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, Guangzhou 510060, Guangdong, China. ; Wen-Jing Guo:1.CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, and Guangzhou Medical University, Guangzhou, Guangdong, China. ; Shi-Hua Pan:1.CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, and Guangzhou Medical University, Guangzhou, Guangdong, China. ; Ying Zhang:1.CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine
期刊:TILRR (FREM1 isoform 2) is a prognostic biomarker correlated with immune infiltration in breast cancer
影响因子 :5.2
引用产品: BT-474 [BT474] 细胞
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