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产品概述
人淋巴管内皮细胞
Cat NO.: CP-H026
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产品名称：人淋巴管内皮细胞
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组织来源：淋巴管
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人淋巴管内皮细胞分离自淋巴管组织；淋巴管由毛细淋巴管汇合而成。其形态结构与静脉相似，但管径较细，管壁较薄，瓣膜较多且发达，外形呈串珠状。淋巴管根据其位置分为浅、深二种。它们管位于皮下，常与浅静脉伴行，收集皮肤和皮下组织的淋巴。深淋巴管与深部血管伴行，收集肌肉和内脏的淋巴。浅、深淋巴管之间有广泛的交通支。淋巴管在向心行程中，通常经过一个或多个淋巴结，从而把淋巴细胞带入淋巴液。主要功能是滤过淋巴液，产生淋巴细胞和浆细胞，参与机体的免疫反应。当局部感染时，细菌、病毒或癌细胞等可沿淋巴管侵入，引起局部淋巴结肿大。如该淋巴结不能阻止和消灭它们，则病变可沿淋巴管的流注方向扩散和转移。淋巴管内皮细胞(LEC)是衬覆于淋巴管内表面的一种单层扁平上皮，是构成淋巴管壁的主要结构，参与维持体液平衡，调节淋巴细胞再循环和机体的免疫反应和组织液及蛋白质的运输，在疾病过程中也起着重要作用。近年研究表明，LEC还在伤口愈合、淋巴管水肿和炎症扩散等病理过程中起重要作用，而且与肿瘤转移密切相关。淋巴管内皮细胞主要功能：①调节体液、蛋白和组织压力平衡；②为免疫系统的重要组成部分。淋巴管内皮细胞与主要病生理变化：①囊肿型淋巴管瘤；②淋巴管炎；③淋巴结核。
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方法简介：
普诺赛实验室分离的人淋巴管内皮细胞采用中性蛋白酶消化法结合内皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人淋巴管内皮细胞经VEGFR3免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 PLL（0.1mg/ml）或明胶（0.1%） 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H026 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 内皮细胞样 传代特性 可传1-2代 消化液 0.25%胰蛋白酶 人淋巴管内皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Lymphatic endothelial cell-mediated accumulation of CD177+Treg cells suppresses antitumor immunity in human esophageal squamous cell carcinoma (2024-03-20)
作者：Min Ma:1.Chronic Disease Laboratory, School of Medicine South China University of Technology, Guangzhou, China. ; 2.Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China. ; Liang Li:1.Medical Research Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China. ; Shu-Han Yang:1.Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China. ; Chuan Huang:1.Chronic Disease Laboratory, School of Medicine South China University of Technology, Guangzhou, China. ; 2.Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China. ; Weitao Zhuang:1.Department of Thoracic Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China. ; Shujie Huan
期刊：Lymphatic endothelial cell-mediated accumulation of CD177+Treg cells suppresses antitumor immunity in human esophageal squamous cell carcinoma
DOI：10.1080/2162402X.2024.2327692
影响因子 ：7.2
引用产品： KYSE-150 细胞 , 人淋巴管内皮细胞 , 多聚-L-赖氨酸溶液 (10×) , 人淋巴管内皮细胞完全培养基
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A Self-Generated Electricity-Driven Drug Delivery System for Precision Management of Myopia (2023-10-28)
作者：Lingxi Jiang: 1.Health Management Center, Sichuan Provincial Key Laboratory for Human Disease Gene Study, Center for Medical Genetics, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, China;2.Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences, Chengdu, Sichuan 610072, China; Luhan Zhang: 1.Health Management Center, Sichuan Provincial Key Laboratory for Human Disease Gene Study, Center for Medical Genetics, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, China; Chao Dai: 1.Health Management Center, Sichuan Provincial Key Laboratory for Human Disease Gene Study, Center for Medical Genetics, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, China; Bo Zhao: 1.Health Management Center, Sichuan Provincial
期刊：A Self-Generated Electricity-Driven Drug Delivery System for Precision Management of Myopia
DOI：10.1016/j.nanoen.2023.109040
影响因子 ：17.6
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CD45− erythroid progenitor cells promote lymph node metastasis in gastric cancer by inducing a hybrid epithelial/mesenchymal state in lymphatic endothelial cells (2023-09-07)
作者：Jing-Yun Wen:1.Department of Pathology, The Third Affiliated Hospital of Sun Yat-sen University, 600 Tianhe Road, Guangzhou, 510630, Guangdong, China. ; 2.Department of Medical Oncology and Guangdong Key Laboratory of Liver Disease Research, The Third Affiliated Hospital of Sun Yat-sen University, 600 Tianhe Road, Guangzhou, 510630, China. ; Xing Li:1.Department of Medical Oncology and Guangdong Key Laboratory of Liver Disease Research, The Third Affiliated Hospital of Sun Yat-sen University, 600 Tianhe Road, Guangzhou, 510630, China. ; Jian-Ning Chen:1.Department of Pathology, The Third Affiliated Hospital of Sun Yat-sen University, 600 Tianhe Road, Guangzhou, 510630, Guangdong, China. ; Jie Chen:1.Department of Medical Oncology and Guangdong Key Laboratory of Liver Disease Research, The Third Affiliated Hospital of Sun Yat-sen University, 600 Tianhe Road, Guangzhou, 510630, China. ; Jing-Yue Zhang:1.Department of Pathology, The Third Affiliated Hospital of Sun Yat-sen University,
期刊：CD45− erythroid progenitor cells promote lymph node metastasis in gastric cancer by inducing a hybrid epithelial/mesenchymal state in lymphatic endothelial cells
DOI：10.1007/s10120-023-01425-x
影响因子 ：7.4
引用产品： 人淋巴管内皮细胞
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Tumor Necrosis Factor-α Promotes the Tumorigenesis, Lymphangiogenesis, and Lymphatic Metastasis in Cervical Cancer via Activating VEGFC-Mediated AKT and ERK Pathways (2023-04-21)
作者：Xiao Chen:1.Department of Gynecology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou 350000, China. ; Luping Lin:1.Department of Abdominal Medical Oncology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou 350000, China. ; Qiaoling Wu:1.Department of Gynecology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou 350000, China. ; Sang Li:1.Department of Gynecology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou 350000, China. ; Huihui Wang:1.Wenzhou Central Hospital, The Second Affiliated Hospital of Shanghai University, China. ; Yang Sun:1.Department of Gynecology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou 350000, China. ;
期刊：Tumor Necrosis Factor-α Promotes the Tumorigenesis, Lymphangiogenesis, and Lymphatic Metastasis in Cervical Cancer via Activating VEGFC-Mediated AKT and ERK Pathways
影响因子 ：4.6
引用产品： 人淋巴管内皮细胞
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PEDF inhibits lymphatic metastasis of nasopharyngeal carcinoma as a new lymphangiogenesis inhibitor (2003-01-20)
作者：Chuanghua Luo:1.Program of Molecular Medicine, Affiliated Guangzhou Women and Children's Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China. ; 2.Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China. ; 3.State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China. ; Haofan Yin:1.Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China. ; Tianxiao Gao:1.State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China. ; Caiqi Ma:1.Program of Molecular Medicine, Affiliated Guangzhou Women and Children's Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China. ; Junxi Liu:1.Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guang
期刊：PEDF inhibits lymphatic metastasis of nasopharyngeal carcinoma as a new lymphangiogenesis inhibitor
DOI：10.1038/s41419-021-03583-1
影响因子 ：9.0
引用产品： 人淋巴管内皮细胞
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Lymphatic Reconstruction in Kidney Allograft Aggravates Chronic Rejection by Promoting Alloantigen Presentation. (2012-01-20)
作者：Jinwen Lin:1.Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. ; Ying Chen:1.Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. ; Huijuan Zhu:1.Department of Pathology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. ; Kai Cheng:1.Xiangya School of Medicine, Central South University, Changsha, China. ; Huiping Wang:1.Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. ; Xianping Yu:1.Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. ; Mengmeng Tang:1.Department of Pathology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. ; Jianghua Chen:1.Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. ;
期刊：Lymphatic Reconstruction in Kidney Allograft Aggravates Chronic Rejection by Promoting Alloantigen Presentation.
影响因子 ：7.3
引用产品： 人淋巴管内皮细胞
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Kallistatin exerts anti-lymphangiogenic effects by inhibiting lymphatic endothelial cell proliferation, migration and tube formation (2004-07-20)
作者：Caiqi Ma:1.Program of Molecular Medicine, Affiliated Guangzhou Women and Children's Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong 510080, P.R. China. ; Haofan Yin:1.Program of Molecular Medicine, Affiliated Guangzhou Women and Children's Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong 510080, P.R. China. ; Jun Zhong:1.Program of Molecular Medicine, Affiliated Guangzhou Women and Children's Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong 510080, P.R. China. ; Yang Zhang:1.Program of Molecular Medicine, Affiliated Guangzhou Women and Children's Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong 510080, P.R. China. ; Chuanghua Luo:1.Department of Biochemistry, Zhongshan Medical School, Sun Yat-Sen University, Guangzhou, Guangdong 510080, P.R. China. ; Di Che:1.Program of Molecular Medicine, Affiliated Guangzhou Women and Children's Hospita
期刊：Kallistatin exerts anti-lymphangiogenic effects by inhibiting lymphatic endothelial cell proliferation, migration and tube formation
影响因子 ：5.2
引用产品： 人淋巴管内皮细胞
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