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产品概述
多聚-L-赖氨酸 （Poly-L-Lysine，PLL）是由赖氨酸单体聚合而成的多聚阳离子，有左旋和右旋两种同分异构，常用左旋的，即PLL。相对分子量为70,000-300,000的PLL可作为细胞培养的生长基质，能够促进细胞粘附和贴壁，是细胞培养中常用的细胞粘附剂。多聚-L-赖氨酸 （PLL）一般常见的分子量有70,000-150,000、150,000-300,000和>300,000，分子量越大，黏附力越强，但相对完全溶解较困难，细胞培养中一般常用分子量150,000-300,000的多聚-L-赖氨酸。
产品信息
形态 | 液体 |
规格 | 10mL |
多聚-L-赖氨酸溶液 | 1 mg/mL |
分子量 | 150,000-300,000 |
溶剂 | 10 mM PBS缓冲液 |
储存条件 | -5~-20℃，避光 |
有效期 | 至少2年 |
运输条件 | 低温 |
使用说明（细胞培养）
1、稀释：用无菌纯化水或PBS溶液9:1稀释该多聚-L-赖氨酸溶液至工作浓度（0.1 mg/mL），稀释的多聚-L-赖氨酸溶液在2-8℃中保存至少3个月有效。
2、包被：放入 37℃培养箱，静置包被 30-60 min以上，注意无菌操作。吸出多聚-L-赖氨酸（建议丢弃；也可留存下次继续使用，建议重复使用不超过3次，多次使用会增加操作污染概率），将培养瓶盖好，放入 37℃培养箱或 4℃冰箱，保持无菌即可；37℃静置 4h以上或者4℃冰箱静置过夜，包被完 成。
3、洗涤：取出包被好的培养瓶，在超净工作台内，用 PBS或培养基清洗 3 次。
4、细胞接种至包被好培养皿中。
5、次日镜下观察细胞的生长状态。
注意事项
1、本产品经过滤除菌，使用本产品时应注意无菌操作，避免污染。
2、使用后的多聚-L-赖氨酸溶液若出现浑浊或污染要丢弃。
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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A radionuclide-labeled nanomedicine for STING pathway activation- and oxygenation-augmented orthotopic cancer radioisotope-immunotherapy (2024-02-01)
作者：Zhisheng Luo: 1.Department of Nuclear Medicine, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha Hunan 410008, China; Ming Zhou: 1.Department of Nuclear Medicine, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha Hunan 410008, China; Ying Peng: 1.Xiangya School of Pharmaceutical Sciences, Central South University, Changsha Hunan, 410013, China; Lu Hao: 1.Department of Nuclear Medicine, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha Hunan 410008, China; Jinhui Yang: 1.Department of Nuclear Medicine, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha Hunan 410008, China; Peng Liu: 1.Department of Nuclear Medicine, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha Hunan 410008, China;2.Key Laboratory of Biological Nanotechnology, NHC. No. 87 Xiangya Road, Changsha Hunan 410008, China; Shuo Hu: 1.Department of Nuclear Medicine, Xiangya Hospital, Central South Universit
期刊：A radionuclide-labeled nanomedicine for STING pathway activation- and oxygenation-augmented orthotopic cancer radioisotope-immunotherapy
影响因子 ：15.1
引用产品： 多聚-L-赖氨酸溶液 (10×)
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Lymphatic endothelial cell-mediated accumulation of CD177+Treg cells suppresses antitumor immunity in human esophageal squamous cell carcinoma (2024-03-20)
作者：Min Ma:1.Chronic Disease Laboratory, School of Medicine South China University of Technology, Guangzhou, China. ; 2.Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China. ; Liang Li:1.Medical Research Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China. ; Shu-Han Yang:1.Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China. ; Chuan Huang:1.Chronic Disease Laboratory, School of Medicine South China University of Technology, Guangzhou, China. ; 2.Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China. ; Weitao Zhuang:1.Department of Thoracic Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China. ; Shujie Huan
期刊：Lymphatic endothelial cell-mediated accumulation of CD177+Treg cells suppresses antitumor immunity in human esophageal squamous cell carcinoma
DOI：10.1080/2162402X.2024.2327692
影响因子 ：7.2
引用产品： KYSE-150 细胞 , 人淋巴管内皮细胞 , 多聚-L-赖氨酸溶液 (10×) , 人淋巴管内皮细胞完全培养基
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The domino effect in inhaled carbon black nanoparticles triggers bloodbrain barrier disruption via altering circulatory inflammation (2022-12-08)
作者：Wenting Cheng: 1.Department of Occupational and Environmental Health, School of Public Health, Qingdao University, Qingdao 266021, China; Wanjun Zhang: 1.Department of Occupational and Environmental Health, School of Public Health, Qingdao University, Qingdao 266021, China; Xiaowen Xia: 1.Department of Occupational and Environmental Health, School of Public Health, Qingdao University, Qingdao 266021, China; Jianzhong Zhang: 1.Department of Occupational and Environmental Health, School of Public Health, Qingdao University, Qingdao 266021, China; Mingyue Wang: 1.Department of Occupational and Environmental Health, School of Public Health, Qingdao University, Qingdao 266021, China; Yanting Li: 1.Department of Occupational and Environmental Health, School of Public Health, Qingdao University, Qingdao 266021, China; Xin Li: 1.Department of Occupational and Environmental Health, School of Public Health, Qingdao University, Qingdao 266021, China; Yuxin Zheng: 1.Department of Occupational and
期刊：The domino effect in inhaled carbon black nanoparticles triggers bloodbrain barrier disruption via altering circulatory inflammation
DOI：10.1016/j.nantod.2022.101721
影响因子 ：19.0
引用产品： 多聚-L-赖氨酸溶液 (10×) , 小鼠脑微血管内皮细胞完全培养基
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Combining a Density Gradient of Biomacromolecular Nanoparticles with Biological Effectors in an Electrospun Fiber-Based Nerve Guidance Conduit to Promote Peripheral Nerve Repair (2022-12-09)
作者：Binghui Jin:1.Beijing Laboratory of Biomedical Materials, State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing, 100029, P. R. China. ; 2.Oujiang Laboratory, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, China. ; Yiling Yu:1.Beijing Laboratory of Biomedical Materials, State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing, 100029, P. R. China. ; Chenghao Lou:1.Oujiang Laboratory, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, China. ; Xiaodi Zhang:1.Beijing Laboratory of Biomedical Materials, State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing, 100029, P. R. China. ; Bowen Gong:1.Beijing Laboratory of Biomedical Materials, State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing, 100029, P. R. China. ; Jinghao Chen:1
期刊：Combining a Density Gradient of Biomacromolecular Nanoparticles with Biological Effectors in an Electrospun Fiber-Based Nerve Guidance Conduit to Promote Peripheral Nerve Repair
影响因子 ：17.5
引用产品： PC-12 (Undifferentiated) 细胞 , 多聚-L-赖氨酸溶液 (10×) , PC-12 (Undifferentiated)细胞专用培养基
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Intestinal Epithelial Cell Exosome Launches IL-1β-Mediated Neuron Injury in Sepsis-Associated Encephalopathy (2001-02-20)
作者：Shaosong Xi:1.Department of Critical Care Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China. ; Yunguang Wang:1.Department of Critical Care Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China. ; Chenghao Wu:1.Department of Critical Care Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China. ; Weihua Peng:1.Department of Critical Care Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China. ; Ying Zhu:1.Department of Critical Care Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China. ; Wei Hu:1.Department of Critical Care Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China. ;
期刊：Intestinal Epithelial Cell Exosome Launches IL-1β-Mediated Neuron Injury in Sepsis-Associated Encephalopathy
影响因子 ：5.5
引用产品： 多聚-L-赖氨酸溶液 (10×)
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T0901317, a liver X receptor agonist, ameliorates perinatal white matter injury induced by ischemia and hypoxia in neonatal rats (2022-11-29)
作者：Ting Gao:1.National Health Commission Key Laboratory of Neonatal Diseases, Department of Neonatology, Children's Hospital of Fudan University, Shanghai 201102, China; Department of Neonatology, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai 201102, China. ; Tianyang Qian:1.National Health Commission Key Laboratory of Neonatal Diseases, Department of Neonatology, Children's Hospital of Fudan University, Shanghai 201102, China. ; Tianwei Wang:1.Department of Neurosurgery, Shanghai Jiaotong University School of Medicine Affiliated Renji Hospital, Shanghai, China. ; Yu Su:1.Department of Neonatology, Children's Hospital of Fudan University and Institutes of Biomedical Sciences, Fudan University, Shanghai, China. ; Han Qiu:1.National Health Commission Key Laboratory of Neonatal Diseases, Department of Neonatology, Children's Hospital of Fudan University, Shanghai 201102, China. ; Wan Tang:1.National Health Commission Key Laboratory of Neonatal Dis
期刊：T0901317, a liver X receptor agonist, ameliorates perinatal white matter injury induced by ischemia and hypoxia in neonatal rats
DOI：10.1016/j.neulet.2022.136994
影响因子 ：3.2
引用产品： DMEM高糖 培养基 , 多聚-L-赖氨酸溶液 (10×)
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