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产品概述
人食管上皮细胞
Cat NO.: CP-H031
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产品名称：人食管上皮细胞
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组织来源：食管组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人食管上皮细胞分离自食管组织；食管是咽和胃之间的消化管，食管在系统发生上起初很短，随着颈部的伸长和心肺的下降，而逐渐增长。食管可分为颈段、胸段和腹段。脊椎动物食管的颈段位于气管背后和脊柱前端，胸段位于左、右肺之间的纵膈内，胸段通过膈孔与腹腔内腹相连，腹段很短与胃相连。在发育过程中，食管的上皮细胞增殖，由单层变为复层，食管使管腔变狭窄，甚至一度闭锁，以后管腔又重新出现。哺乳动物的食管结构上由内向外分四层：黏膜层、黏膜下层、肌层和外膜。其中，黏膜层，包括上皮、固有层和黏膜肌层。上皮为较厚的未角化的复层扁平上皮，耐摩擦，有保护作用。在食管与胃贲门交界处，复层扁平上皮突然变成单层柱状上皮。食管被一层线性排列的、无角化、潮湿的复层扁平上皮细胞覆盖，其顶端细胞膜和细胞间连接复合体联合在一起产生有效渗透屏障，防止食管内容物的渗入。特别的是，层屏障使表层细胞的基质外侧细胞膜和深层细胞的全部细胞膜不暴露于食管腔内规律的大幅波动的渗透压之下。从组织学上讲，食管上皮由两层组成，基底层和分化层；其中，仅基底层的细胞可以增殖，然后向食管腔移行。移行过程伴随有分化的发生和分化标记的依序表达。食管上皮细胞的培养是研究食管正常生理机制和食管癌变机制的非常好的体外模型。
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方法简介：
普诺赛实验室分离的人食管上皮细胞采用机械分离法结合组织贴块法，并通过上皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人食管上皮细胞经PCK免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 鼠尾胶原Ⅰ(2-5μg/cm2) 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H031 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 上皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 人食管上皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Artesunate-loaded solid lipid nanoparticles resist esophageal squamous cell carcinoma by inducing Ferroptosis through inhibiting the AKT/mTOR signaling (2024-02-16)
作者：Yu Xia:1.College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350000, China; Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou 350001, China. ; Yixin Tang:1.College of Chemical Engineering, Fuzhou University, Fuzhou 350108, China; Engineering Research Center of Advanced Manufacturing Technology for Fine Chemicals, College of Chemical Engineering, Fuzhou University, Fuzhou 350108, China. ; Zhixin Huang:1.College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350000, China; Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou 350001, China. ; Nantian Ke:1.Shengli Clinical Medical College, Fujian Medical University, Fuzhou 350001, China; Department of Clinical Laboratory, Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350001, China. ; Yue Zheng:1.Department of Clinical Laboratory, Fujian Provincial Hospital, Fuzhou 350001, China; Sheng
期刊：Artesunate-loaded solid lipid nanoparticles resist esophageal squamous cell carcinoma by inducing Ferroptosis through inhibiting the AKT/mTOR signaling
DOI：10.1016/j.cellsig.2024.111108
影响因子 ：4.8
引用产品： KYSE-150 细胞 , KYSE-30 细胞 , 人食管上皮细胞 , RPMI-1640完全培养基 (含10%FBS）
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ZPR1 is an immunodiagnostic biomarker and promotes tumor progression in esophageal squamous cell carcinoma (2023-11-14)
作者：Guiying Sun:1.Department of Epidemiology and Statistics, College of Public Health, Zhengzhou University, Zhengzhou, China. ; 2.Henan Key Laboratory of Tumor Epidemiology and State Key Laboratory of Esophageal Cancer Prevention and Treatment, Zhengzhou University, Zhengzhou, China. ; Hua Ye:1.Department of Epidemiology and Statistics, College of Public Health, Zhengzhou University, Zhengzhou, China. ; 2.Henan Key Laboratory of Tumor Epidemiology and State Key Laboratory of Esophageal Cancer Prevention and Treatment, Zhengzhou University, Zhengzhou, China. ; Huijuan Liu:1.Scientific Research Department, The First Affiliated Hospital of Henan University of CM, Henan University of Chinese Medicine, Zhengzhou, China. ; Tiandong Li:1.Department of Epidemiology and Statistics, College of Public Health, Zhengzhou University, Zhengzhou, China. ; 2.Henan Key Laboratory of Tumor Epidemiology and State Key Laboratory of Esophageal Cancer Prevention and Treatment, Zhengzhou University, Zhengzhou
期刊：ZPR1 is an immunodiagnostic biomarker and promotes tumor progression in esophageal squamous cell carcinoma
影响因子 ：5.7
引用产品： KYSE-150 细胞 , Eca-109 细胞 , TE-1 细胞 , 人食管上皮细胞
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Circ_0024108 promotes the progression of esophageal cancer cells (2023-02-09)
作者：Tongchen Hu:1.Department of Thoracic Surgery, The Affiliated Hospital of Southwest Medical University, No. 25 TaiPing St, Jiangyang District, Luzhou, 646000, Sichuan, People's Republic of China. ; Huali Peng:1.Department of Thoracic Surgery, The People's Hospital of Leshan, Leshan, 614000, Sichuan, China. ; Fan Yang:1.Department of Thoracic Surgery, The People's Hospital of Leshan, Leshan, 614000, Sichuan, China. ; Fan Zhang:1.Department of Thoracic Surgery, The People's Hospital of Leshan, Leshan, 614000, Sichuan, China. ; Jintao He:1.Department of Thoracic Surgery, The Affiliated Hospital of Southwest Medical University, No. 25 TaiPing St, Jiangyang District, Luzhou, 646000, Sichuan, People's Republic of China. hrm121212@163.com. ;
期刊：Circ_0024108 promotes the progression of esophageal cancer cells
DOI：10.1007/s11748-023-01909-8
影响因子 ：1.2
引用产品： Eca-109 细胞 , KYSE-150 细胞 , KYSE450 细胞 , KYSE-410 细胞 , 人食管上皮细胞
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CircGFPT1 regulates the growth and apoptosis of esophageal squamous cell carcinoma through miR-142-5p/HAX1 axis (2023-07-16)
作者：Zheng Feng:1.Department of Thoracic Surgery, Tangdu Hospital of Air Force Military Medical University, No. 1, Xinsi Road, Baqiao District, Xi'an City, 710000, Shaanxi, China. ; Tianyi Zhang:1.Department of Thoracic Surgery, Tangdu Hospital of Air Force Military Medical University, No. 1, Xinsi Road, Baqiao District, Xi'an City, 710000, Shaanxi, China. ; Shaoyi Cheng:1.Department of Thoracic Surgery, Tangdu Hospital of Air Force Military Medical University, No. 1, Xinsi Road, Baqiao District, Xi'an City, 710000, Shaanxi, China. ; Xunliang Yin:1.Department of Thoracic Surgery, Tangdu Hospital of Air Force Military Medical University, No. 1, Xinsi Road, Baqiao District, Xi'an City, 710000, Shaanxi, China. ; Yongan Zhou:1.Department of Thoracic Surgery, Tangdu Hospital of Air Force Military Medical University, No. 1, Xinsi Road, Baqiao District, Xi'an City, 710000, Shaanxi, China. wqqmez@163.com. ;
期刊：CircGFPT1 regulates the growth and apoptosis of esophageal squamous cell carcinoma through miR-142-5p/HAX1 axis
DOI：10.1007/s11748-023-01955-2
影响因子 ：1.2
引用产品： KYSE-150 细胞 , 人食管上皮细胞
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Exploring the Mechanism of Realgar against Esophageal Cancer Based on Ferroptosis Induced by ROS-ASK1-p38 MAPK Signaling Pathway (2022-09-12)
作者：Ruyi Yang:1.Department of Integrative Medicine, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai, China. ; Fazhang Chen:1.Medical College of Qinghai University, Xining, China. ; Haizhen Xu:1.Department of Integrative Medicine, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai, China. ; Zhanfang Guo:1.Department of Integrative Medicine, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai, China. ; Changxia Cao:1.Department of Integrative Medicine, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai, China. ; Hongyan Zhang:1.Department of Integrative Medicine, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai, China. ; Changrong Zhang:1.Department of Integrative Medicine, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai, China. ;
期刊：Exploring the Mechanism of Realgar against Esophageal Cancer Based on Ferroptosis Induced by ROS-ASK1-p38 MAPK Signaling Pathway
影响因子 ：2.7
引用产品： Eca-109 细胞 , KYSE-150 细胞 , 人食管上皮细胞
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