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产品概述
名称 | EA.hy926 (人脐静脉细胞融合细胞) (STR鉴定正确) |
别称 | EA. hy 926; EA hy 926; EAhy 926; EAHY-926; EA.Hy926; EA.hy926; EAhy926; EaHy926; Eahy926 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | EA.hy926细胞是在PEG胁迫下,将原代培养的人脐静脉细胞与抗硫鸟嘌呤的A549克隆株融合,构建的一株人脐静脉细胞株。融合子在HAT培养基上生长,并以Ⅷ因子相关抗原筛选,EA.hy926细胞已经过100次以上的群体倍增(PDLs)。电镜照片显示,Weibel-Palade小体的细胞质分布以及组织特异性的细胞器,分化的内皮细胞特性如血管生成,体内平衡-血栓形成,血压及炎症反应。 |
组织来源 | 脐静脉/体细胞杂交 |
细胞类型 | 杂交瘤细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~12-24 hours |
抗原表达情况 | Factor VIII-related antigen; Homo sapiens, expressed |
基因表达情况 | Factor VIII-related antigen; Homo sapiens. |
保藏机构 | ATCC; CRL-2922 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10,11,12 D2S1338 22,24 D3S1358 15,16 D5S818 11 D7S820 8,9,10 D8S1179 13 D13S317 11 D16S539 11,12 D18S51 13,15,17 D19S433 13,14 D21S11 28,29,32 FGA 22,23 PentaD 9,11 PentaE 7,11,12 TH01 6,8,9.3 TPOX 8,9 vWA 14,17 D6S1043 11,12,22 D12S391 15,18 D2S441 10,11,14 -
STR鉴定图
参考文献
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Chondroitin sulfate-modified antiangiogenic peptide conjugate induces cell apoptosis via the mitochondria-mediated pathway to perform antitumor activity (2024-02-29)
作者:Yan Li:1.National Glycoengineering Research Center, Shandong University, Qingdao 266237, China; NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, Shandong University, Qingdao 266237, China; Shandong Provincial Technology Innovation Center of Carbohydrate, Shandong University, Qingdao 266237, China. ; Jiaai Fu:1.National Glycoengineering Research Center, Shandong University, Qingdao 266237, China; NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, Shandong University, Qingdao 266237, China; Shandong Provincial Technology Innovation Center of Carbohydrate, Shandong University, Qingdao 266237, China. ; Huiwen Hou:1.National Glycoengineering Research Center, Shandong University, Qingdao 266237, China; NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, Shandong University, Qingdao 266237, China; Shandong Provincial Technology Innovation Center of Carbohydrate, Shandong Universit
期刊:Chondroitin sulfate-modified antiangiogenic peptide conjugate induces cell apoptosis via the mitochondria-mediated pathway to perform antitumor activity
DOI:10.1016/j.ijbiomac.2024.129671
影响因子 :8.2
引用产品: EA.hy926 细胞
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Human umbilical cord mesenchymal stem cells overexpressing RUNX1 promote tendon-bone healing by inhibiting osteolysis, enhancing osteogenesis and promoting angiogenesis (2024-01-05)
作者:Dan Guo:1.Department of Orthopedic, Yangzhou Clinical Medical College of Nanjing Medical University, Yangzhou, 225001, Jiangsu, China. ; Jian Yang:1.Department of Orthopedic, Yangzhou Clinical Medical College of Nanjing Medical University, Yangzhou, 225001, Jiangsu, China. ; Dianwei Liu:1.Department of Orthopedic, Yangzhou Clinical Medical College of Nanjing Medical University, Yangzhou, 225001, Jiangsu, China. ; Pei Zhang:1.Department of Orthopedic, Yangzhou Clinical Medical College of Nanjing Medical University, Yangzhou, 225001, Jiangsu, China. ; Hao Sun:1.Department of Orthopedic, Yangzhou Clinical Medical College of Nanjing Medical University, Yangzhou, 225001, Jiangsu, China. ; Jingcheng Wang:1.Department of Orthopedic, Yangzhou Clinical Medical College of Nanjing Medical University, Yangzhou, 225001, Jiangsu, China. wangjcyangzhou@163.com. ;
期刊:Human umbilical cord mesenchymal stem cells overexpressing RUNX1 promote tendon-bone healing by inhibiting osteolysis, enhancing osteogenesis and promoting angiogenesis
DOI:10.1007/s13258-023-01478-3
影响因子 :2.1
引用产品: THP-1 细胞 , 人脐带间充质干细胞 , EA.hy926 细胞 , 青霉素-链霉素溶液 (双抗),100× , 人脐带间充质干细胞成骨诱导分化培养基
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Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation (2023-10-13)
作者:Jiayu Liu:1.Department of Physiology and Pathophysiology, School of Basic Medical Sciences, State Laboratory of Vascular Homeostasis and Remodeling, Peking University, Beijing, 100191, China. ; 2.National Health Commission Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Beijing Key Laboratory of Cardiovascular Receptors Research, Peking University, Beijing, 100191, China. ; Chuanrong Zhao:1.Department of Physiology and Pathophysiology, School of Basic Medical Sciences, State Laboratory of Vascular Homeostasis and Remodeling, Peking University, Beijing, 100191, China. ; 2.National Health Commission Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Beijing Key Laboratory of Cardiovascular Receptors Research, Peking University, Beijing, 100191, China. ; 3.Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, China. ; Xue Xiao:1.National Labo
期刊:Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation
DOI:10.1038/s41467-023-42341-z
影响因子 :16.6
引用产品: EA.hy926 细胞 , NIH/3T3 细胞
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CD44 targeting nanodrug based on chondroitin sulfate for melanoma therapy by inducing mitochondrial apoptosis pathways (2023-08-02)
作者:Yan Li:1.National Glycoengineering Research Center, Shandong University, Qingdao 266237, China; NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, Shandong University, Qingdao 266237, China; Shandong Provincial Technology Innovation Center of Carbohydrate, Shandong University, Qingdao 266237, China. ; Huiwen Hou:1.National Glycoengineering Research Center, Shandong University, Qingdao 266237, China; NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, Shandong University, Qingdao 266237, China; Shandong Provincial Technology Innovation Center of Carbohydrate, Shandong University, Qingdao 266237, China. ; Zengmei Liu:1.National Glycoengineering Research Center, Shandong University, Qingdao 266237, China; NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, Shandong University, Qingdao 266237, China; Shandong Provincial Technology Innovation Center of Carbohydrate, Shandong Univer
期刊:CD44 targeting nanodrug based on chondroitin sulfate for melanoma therapy by inducing mitochondrial apoptosis pathways
DOI:10.1016/j.carbpol.2023.121255
影响因子 :11.2
引用产品: EA.hy926 细胞 , B16 细胞
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Fabrication and performance evaluation of PLCL-hCOLIII small-diameter vascular grafts crosslinked with procyanidins (2023-08-15)
作者:Han Wang:1.School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, 100081, China; National Institute for Food and Drug Control, Beijing 102629, China. ; Yonghao Xiao:1.School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, 100081, China. ; Zhiping Fang:1.School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, 100081, China. ; Yuanguo Zhang:1.Department of Thyroid-Breast-Vascular Surgery, Shanxian Central Hospital, Heze, Shandong 274300, China. ; Liu Yang:1.National Institute for Food and Drug Control, Beijing 102629, China. ; Chenyu Zhao:1.National Institute for Food and Drug Control, Beijing 102629, China. ; Zhu Meng:1.National Institute for Food and Drug Control, Beijing 102629, China. ; Yu Liu:1.National Institute for Food and Drug Control, Beijing 102629, China; Yantai University, Yantai, Shandong 264005, China. ; Chongchong Li:1.National Institute for Food and Drug Control, Beijin
期刊:Fabrication and performance evaluation of PLCL-hCOLIII small-diameter vascular grafts crosslinked with procyanidins
DOI:10.1016/j.ijbiomac.2023.126293
影响因子 :8.2
引用产品: EA.hy926 细胞
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Biomimetic nanoplatform with selectively positioned indocyanine green for accurate sentinel lymph node imaging (2023-11-16)
作者:Wenjing Cheng:1.Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, China. ; Xiangbai Wu:1.Hubei Provincial Clinical Research Center for Precise Prevention and Treatment of Elderly Gastrointestinal Cancer, The Second People's Hospital of China Three Gorges University, Yichang, China. ; Shi Yu:1.Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, China. ; Chengwei Zhang:1.Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, China. ; Yinhong Song:1.Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, China. ; Xinzhi Li:1.Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, China. ; Xiang Yu:1.School of Biomedical Engineering, Hainan University, Haikou, Hainan 570228, China. yuxiang@hainanu.edu.cn. ; 2.Key Laboratory of
期刊:Biomimetic nanoplatform with selectively positioned indocyanine green for accurate sentinel lymph node imaging
影响因子 :6.7
引用产品: RAW 264.7 细胞 , EA.hy926 细胞
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PLA-HPG based coating enhanced anti-biofilm and wound healing of Shikonin in MRSA-infected burn wound (2023-08-10)
作者:Huiyu Han:1.Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China. ; Lianheng Chen:1.Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China. ; Shu Liang:1.Center Lab of Longhua Branch, Department of Infectious Disease, Shenzhen People's Hospital, The Second Clinical Medical College, Jinan University, Southern University of Science and Technology, Shenzhen, Guangdong, China. ; 2.School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen, China. ; Jiawei Lü:1.Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China. ; Yashi Wu:1.Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China. ; Xiongjun Wang:1.Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China. ; Fei Xu:1.Department of Plastic Surgery, Naval Medical Center, Naval Medical University, Sh
期刊:PLA-HPG based coating enhanced anti-biofilm and wound healing of Shikonin in MRSA-infected burn wound
DOI:10.3389/fbioe.2023.1243525
影响因子 :5.7
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Study on the effect of type III recombinant humanized collagen on human vascular endothelial cells (2023-11-29)
作者:Han Wang:1.Department of Medical Devices, National Institute for Food and Drug Control, Beijing, China. ; Yonghao Xiao:1.School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, China. ; Yuanguo Zhang:1.Department of Thyroid-Breast-Vascular Surgery, Shanxian Central Hospital, Heze, Shandong, China. ; Zhu Meng:1.Department of Medical Devices, National Institute for Food and Drug Control, Beijing, China. ; Chenyu Zhao:1.Department of Medical Devices, National Institute for Food and Drug Control, Beijing, China. ; Fanshan Qiu:1.Department of Medical Devices, National Institute for Food and Drug Control, Beijing, China. ; Chongchong Li:1.Department of Medical Devices, National Institute for Food and Drug Control, Beijing, China. ; Zengguo Feng:1.School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, China. ;
期刊:Study on the effect of type III recombinant humanized collagen on human vascular endothelial cells
影响因子 :3.0
引用产品: EA.hy926 细胞
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Modification of the small intestinal submucosa membrane with oligopeptides screened from intrinsically disordered regions to promote angiogenesis and accelerate wound healing (2023-03-02)
作者:Shiqing Ma:1.Department of Stomatology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China. ; Yilin Yang:1.School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, China. ; Yuzhu Mu:1.School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, China. ; Huizhen Peng:1.The First Affiliated Hospital of Jinan University, School of Stomatology, Clinical Research Platform for Interdiscipline of Stomatology, Jinan University, Guangzhou 510630, China. ; Pengfei Wei:1.Beijing Biosis Healing Biological Technology Co., Ltd, Beijing 102600, China. ; Wei Jing:1.Beijing Biosis Healing Biological Technology Co., Ltd, Beijing 102600, China; Foshan (Southern China) Institute for New Materials, Foshan 528220, China. ; Cheng Peng:1.Department of Stomatology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China. ; Xiangning Liu:1.The First Affiliated Hospital of Jinan University, School of Stomatology, Clinical Res
期刊:Modification of the small intestinal submucosa membrane with oligopeptides screened from intrinsically disordered regions to promote angiogenesis and accelerate wound healing
DOI:10.1016/j.bioadv.2023.213360
影响因子 :0.0
引用产品: EA.hy926 细胞
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Core–Shell Filament with Excellent Wound Healing Property Made of Cellulose Nanofibrils and Guar Gum via Interfacial Polyelectrolyte Complexation Spinning (2022-11-26)
作者:Meiyan Wu:1.CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China. ; 2.Shandong Energy Institute, Qingdao, 266101, China. ; 3.Qingdao New Energy Shandong Laboratory, Qingdao, 266101, China. ; Yinuo Liu:1.CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China. ; Chao Liu:1.CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China. ; Qiu Cui:1.CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China. ; Xin Zheng:1.Qingdao Hospital of Traditional Chinese Medicine (Municipal Hiser Hospital), Qingdao, 266033, China. ; Pedram Fatehi:1.Green Processes Research Centre and Biorefining Research Institute, Lakehead University, Thunder Bay, ON P7B5E1, Can
期刊:Core–Shell Filament with Excellent Wound Healing Property Made of Cellulose Nanofibrils and Guar Gum via Interfacial Polyelectrolyte Complexation Spinning
影响因子 :15.2
引用产品: EA.hy926 细胞 , BRL 细胞
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A dual Keap1 and p47phox inhibitor Ginsenoside Rb1 ameliorates high glucose/ox-LDL-induced endothelial cell injury and atherosclerosis (2022-09-26)
作者:Zi-Chao Wang:1.State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 210009, China. ; 2.School of Traditional Chinese Pharmacy, China Pharmaceutical University, No. 639 Longmian Road, Nanjing, 211198, China. ; Kai-Ming Niu:1.State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 210009, China. ; 2.School of Traditional Chinese Pharmacy, China Pharmaceutical University, No. 639 Longmian Road, Nanjing, 211198, China. ; Yu-Jie Wu:1.State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 210009, China. ; 2.School of Traditional Chinese Pharmacy, China Pharmaceutical University, No. 639 Longmian Road, Nanjing, 211198, China. ; Kai-Rui Du:1.State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 210009, China. ; L
期刊:A dual Keap1 and p47phox inhibitor Ginsenoside Rb1 ameliorates high glucose/ox-LDL-induced endothelial cell injury and atherosclerosis
DOI:10.1038/s41419-022-05274-x
影响因子 :9.7
引用产品: EA.hy926 细胞
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Carboxy-terminal telopeptide levels of type I collagen hydrogels modulated the encapsulated cell fate for regenerative medicine (2022-12-22)
作者:Chuan Niu:1.Department of Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, People's Republic of China. ; Ying Xiong:1.Department of Periodical Press, West China Hospital, Sichuan University, Chengdu, People's Republic of China. ; Liping Yang:1.Department of Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, People's Republic of China. ; Xiong Xiao:1.Department of Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, People's Republic of China. ; Shaojie Yang:1.Department of Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, People's Republic of China. ; Ziwei Huang:1.Department of Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, People's Republic of China. ; Yuchu Yang:1.Department of Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, People's Republic of
期刊:Carboxy-terminal telopeptide levels of type I collagen hydrogels modulated the encapsulated cell fate for regenerative medicine
DOI:10.1016/j.ijbiomac.2022.12.186
影响因子 :8.0
引用产品: EA.hy926 细胞
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Osteo-angiogenic and antibacterial activity of a multifunctional micro-porous coating on zirconium alloy (2022-08-06)
作者:Xiaoning Xu: 1.School of Material Science and Engineering, Central South University, Changsha 410083, China; Xiaohui Sun: 1.School of Material Science and Engineering, Central South University, Changsha 410083, China; Xinrong Tan: 1.School of Materials Science and Engineering, Hunan University of Science and Technology, Xiangtan 411201, China; Renhao Xue: 1.School of Material Science and Engineering, Central South University, Changsha 410083, China; Ligang Zhang: 1.School of Material Science and Engineering, Central South University, Changsha 410083, China; Libin Liu: 1.School of Material Science and Engineering, Central South University, Changsha 410083, China;2.State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China;
期刊:Osteo-angiogenic and antibacterial activity of a multifunctional micro-porous coating on zirconium alloy
DOI:10.1016/j.apsusc.2022.154465
影响因子 :7.4
引用产品: MC3T3-E1 Subclone 24 细胞 , EA.hy926 细胞
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Platelet-rich plasma promotes diabetic ulcer repair through inhibition of ferroptosis (2022-10-01)
作者:Li Chen:1.Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China. ; 2.Department of Blood Transfusion, The First Affiliated Hospital Bengbu Medical College, Bengbu, China. ; Daoai Wu:1.Department of Endocrinology, The First Affiliated Hospital Bengbu Medical College, Bengbu, China. ; Lili Zhou:1.Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China. ; 2.Department of Hematology, The First Affiliated Hospital Bengbu Medical College, Bengbu, China. ; Yan Ye:1.Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China. ;
期刊:Platelet-rich plasma promotes diabetic ulcer repair through inhibition of ferroptosis
影响因子 :3.6
引用产品: 人真皮成纤维细胞 , EA.hy926 细胞
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Chimeric Peptides Quickly Modify the Surface of Personalized 3D Printing Titanium Implants to Promote Osseointegration (2007-01-20)
作者:Zhezhe Zhao:1.School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, People's Republic of China. ; Shiqing Ma:1.School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, People's Republic of China. ; Chenxuan Wu:1.School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, People's Republic of China. ; Xuewen Li:1.School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, People's Republic of China. ; Xinying Ma:1.School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, People's Republic of China. ; Han Hu:1.School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, People's Republic of China. ; Jie Wu:1.School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, People's Republic of China. ; Yonglan Wang:1.School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, People's Republic of China. ; Zihao Liu:1.S
期刊:Chimeric Peptides Quickly Modify the Surface of Personalized 3D Printing Titanium Implants to Promote Osseointegration
影响因子 :9.5
引用产品: EA.hy926 细胞
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