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产品概述
名称 | EA.hy926 (人脐静脉细胞融合细胞) (STR鉴定正确) |
别称 | EA. hy 926; EA hy 926; EAhy 926; EAHY-926; EA.Hy926; EA.hy926; EAhy926; EaHy926; Eahy926 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | EA.hy926细胞是在PEG胁迫下,将原代培养的人脐静脉细胞与抗硫鸟嘌呤的A549克隆株融合,构建的一株人脐静脉细胞株。融合子在HAT培养基上生长,并以Ⅷ因子相关抗原筛选,EA.hy926细胞已经过100次以上的群体倍增(PDLs)。电镜照片显示,Weibel-Palade小体的细胞质分布以及组织特异性的细胞器,分化的内皮细胞特性如血管生成,体内平衡-血栓形成,血压及炎症反应。 |
组织来源 | 脐静脉/体细胞杂交 |
细胞类型 | 杂交瘤细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~12-24 hours |
抗原表达情况 | Factor VIII-related antigen; Homo sapiens, expressed |
基因表达情况 | Factor VIII-related antigen; Homo sapiens. |
保藏机构 | ATCC; CRL-2922 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10,11,12 D2S1338 22,24 D3S1358 15,16 D5S818 11 D7S820 8,9,10 D8S1179 13 D13S317 11 D16S539 11,12 D18S51 13,15,17 D19S433 13,14 D21S11 28,29,32 FGA 22,23 PentaD 9,11 PentaE 7,11,12 TH01 6,8,9.3 TPOX 8,9 vWA 14,17 D6S1043 11,12,22 D12S391 15,18 D2S441 10,11,14 -
STR鉴定图
参考文献
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Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation(2023/10/13)
作者:Jiayu Liu, Chuanrong Zhao, Xue Xiao
期刊:Nature Communications
DOI:10.1038/s41467-023-42341-z
影响因子 :16.600
引用产品:CL-0272
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Core–Shell Filament with Excellent Wound Healing Property Made of Cellulose Nanofibrils and Guar Gum via Interfacial Polyelectrolyte Complexation Spinning(2022/11/26)
作者:Meiyan Wu, Yinuo Liu, Chao Liu, Qiu Cui, Xin Zheng, Pedram Fatehi, Bin Li
期刊:Small
影响因子 :13.300
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CD44 targeting nanodrug based on chondroitin sulfate for melanoma therapy by inducing mitochondrial apoptosis pathways(2023/08/02)
作者:Yan Li, Huiwen Hou, Zengmei Liu
期刊:CARBOHYDRATE POLYMERS
DOI:10.1016/j.carbpol.2023.121255
影响因子 :11.200
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Chimeric Peptides Quickly Modify the Surface of Personalized 3D Printing Titanium Implants to Promote Osseointegration(2021/07/14)
作者:Zhezhe Zhao, Shiqing Ma, Chenxuan Wu
期刊:ACS Applied Materials & Interfaces
影响因子 :9.500
引用产品:CL-0272
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A dual Keap1 and p47phox inhibitor Ginsenoside Rb1 ameliorates high glucose/ox-LDL-induced endothelial cell injury and atherosclerosis(2022/09/26)
作者:Zichao Wang, Kaiming Niu, Yujie Wu
期刊:Cell Death & Disease
DOI:10.1038/s41419-022-05274-x
影响因子 :9.000
引用产品:CL-0272
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Chondroitin sulfate-modified antiangiogenic peptide conjugate induces cell apoptosis via the mitochondria-mediated pathway to perform antitumor activity(2024/02/29)
作者:Yan Li, Jiaai Fu, Huiwen Hou
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2024.129671
影响因子 :8.200
引用产品:CL-0272
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Carboxy-terminal telopeptide levels of type I collagen hydrogels modulated the encapsulated cell fate for regenerative medicine(2022/12/22)
作者:Chuan Niu, Ying Xiong, Liping Yang
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2022.12.186
影响因子 :8.200
引用产品:CL-0272
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Dynamic RGD ligands derived from highly mobile cyclodextrins regulate spreading and proliferation of endothelial cells to promote vasculogenesis(2024/04/16)
作者:Xuanshu Zhong, Shulei Zhang, Han Wang
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2024.131667
影响因子 :8.200
引用产品:CL-0272
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Fabrication and performance evaluation of PLCL-hCOLIII small-diameter vascular grafts crosslinked with procyanidins(2023/08/15)
作者:Han Wang, Yonghao Xiao, Zhiping Fang
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2023.126293
影响因子 :8.200
引用产品:CL-0272
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Enhancing cellular behavior in repaired tissue via silk fibroin-integrated triboelectric nanogenerators(2024/05/24)
作者:Zhelin Li, Shuxing Xu, Zijie Xu
期刊:Microsystems & Nanoengineering
DOI:10.1038/s41378-024-00694-5
影响因子 :7.900
引用产品:CL-0272
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Biomimetic nanoplatform with selectively positioned indocyanine green for accurate sentinel lymph node imaging(2023/11/16)
作者:Wenjing Cheng, Xiangbai Wu, Shi Yu
期刊:Nanoscale
影响因子 :6.700
引用产品:CL-0272
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Osteo-angiogenic and antibacterial activity of a multifunctional micro-porous coating on zirconium alloy(2022/08/06)
作者:Xiaoning Xu, Xiaohui Sun, Xinrong Tan
期刊:APPLIED SURFACE SCIENCE
DOI:10.1016/j.apsusc.2022.154465
影响因子 :6.700
引用产品:CL-0272
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Osteo-angiogenic activity of a micro/nano hierarchical SrSi-codoped hydroxyapatite coating on zirconium alloy(2024/05/13)
作者:Xiaoning Xu, Kaige Wang, Xinrong Tan
期刊:Journal of Materials Research and Technology-JMR&T
DOI:10.1016/j.jmrt.2024.05.067
影响因子 :6.400
引用产品:CL-0272
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Salvianolic Acid Ameliorates Pressure Overload-Induced Cardiac Endothelial Dysfunction via Activating HIF1α/HSF1/CD31 Pathway(2022/09/17)
作者:Na Li, Weijian Hang, Hongyang Shu
期刊:AMERICAN JOURNAL OF CHINESE MEDICINE
影响因子 :6.005
引用产品:CL-0272
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PLA-HPG based coating enhanced anti-biofilm and wound healing of Shikonin in MRSA-infected burn wound(2023/08/10)
作者:Huiyu Han, Lianheng Chen, Shu Liang
期刊:Frontiers in Bioengineering and Biotechnology
DOI:10.3389/fbioe.2023.1243525
影响因子 :5.700
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Porphyromonas gingivalis OMVs promoting endothelial dysfunction via the STING pathway in periodontitis(2024/05/02)
作者:Zhenwei Chen, Ye Cao, Wenxiu Jiang
期刊:ORAL DISEASES
影响因子 :3.800
引用产品:CL-0272
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Platelet-rich plasma promotes diabetic ulcer repair through inhibition of ferroptosis(2022/10/01)
作者:Li Chen, Daoai Wu, Lili Zhou
期刊:Annals of Translational Medicine
影响因子 :3.616
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Study on the effect of type III recombinant humanized collagen on human vascular endothelial cells(2023/11/29)
作者:Dr. Han Wang, Dr. Yonghao Xiao, Dr. Yuanguo Zhang
期刊:Tissue Engineering Part C-Methods
影响因子 :3.000
引用产品:CL-0272
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Effect of High Magnesium and Astragaloside IV on Vascular Endothelial Cells(2024/05/09)
作者:Hongfei Qi, Teng Ge, Kun Wang
期刊:CELL BIOCHEMISTRY AND BIOPHYSICS
DOI:10.1007/s12013-024-01250-8
影响因子 :2.600
引用产品:CL-0272
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Human umbilical cord mesenchymal stem cells overexpressing RUNX1 promote tendon-bone healing by inhibiting osteolysis, enhancing osteogenesis and promoting angiogenesis(2024/01/05)
作者:Dan Guo, Jian Yang, Dianwei Liu
期刊:Genes & Genomics
DOI:10.1007/s13258-023-01478-3
影响因子 :2.100
引用产品:CL-0272
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