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产品概述
名称 | B16 (小鼠黑色素瘤细胞) (STR鉴定正确) |
别称 | B-16; B16 melanoma; B16 subline B78; B78 |
种属 | C57BL/6小鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:4-1:8 |
推荐换液频率 | 2-3次/周 |
注意事项 | B16系列细胞,使用DMEM培养时可能出现黑色素快速积累,细胞不增殖或死亡的情况,该细胞正常扩增冻存请使用RPMI-1640完培,若您需要进行分泌黑色素等后续相关实验,可以在扩增冻存细胞后更换为DMEM培养来满足实验设计。 |
背景描述 | B16细胞是一株小鼠黑素瘤细胞。 |
年龄(性别) | 不详 |
组织来源 | 黑素瘤 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 黑色素瘤细胞 |
生物安全等级 | BSL-1 |
保藏机构 | JCRB; JCRB0202 KCB; KCB 93030YJ RCB; RCB1283 TKG; TKG 0144 |
STR鉴定
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STR位点信息
18-3 15,16 6-7 15,16 5-5 16,20 X-1 28 1-2 19,20 7-1 26.2 8-1 16,17 1-1 17,18 19-2 13 15-3 22.3,23.3 12-1 17,18 6-4 18,19 4-2 20.3,21.3 3-2 14,15 2-1 16 13-1 17.1,18.1
11-2 16,17 17-2 15,16,17 -
STR鉴定图
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参考文献
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ATP-adenosine axis regulation combined with microneedle assisted photoimmunotherapy to boost the immunotherapy efficiency (2024-01-23)
期刊:JOURNAL OF CONTROLLED RELEASE
DOI:10.1016/j.jconrel.2024.01.035
影响因子 :10.8
引用产品: B16 细胞
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CD44 targeting nanodrug based on chondroitin sulfate for melanoma therapy by inducing mitochondrial apoptosis pathways (2023-08-02)
期刊:CARBOHYDRATE POLYMERS
DOI:10.1016/j.carbpol.2023.121255
影响因子 :11.2
引用产品: EA.hy926 细胞 , B16 细胞
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Microneedle-assisted vaccination combined with autophagy regulation for antitumor immunotherapy (2023-04-24)
期刊:JOURNAL OF CONTROLLED RELEASE
DOI:10.1016/j.jconrel.2023.04.031
影响因子 :10.8
引用产品: B16 细胞
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Endogenous/Exogenous Nanovaccines Synergistically Enhance Dendritic Cell-Mediated Tumor Immunotherapy (2023-02-18)
期刊:Advanced Healthcare Materials
影响因子 :10.0
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Delivery of Mrna Vaccine with 1, 2-diesters-derived Lipids Elicits Fast Liver Clearance for Safe and Effective Cancer Immunotherapy. (2023-11-21)
期刊:Advanced Healthcare Materials
影响因子 :10.0
引用产品: B16 细胞 , RM-1 细胞 , DC2.4 细胞 , 293 [HEK-293] 细胞
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Synthesis of iron-boride/carbon-nitride composites and their applications in chemodynamic therapy (2023-12-14)
期刊:JOURNAL OF COLLOID AND INTERFACE SCIENCE
DOI:10.1016/j.jcis.2023.12.078
影响因子 :9.9
引用产品: B16 细胞
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Janus-Inspired Core–Shell Structure Hydrogel Programmatically Releases Melatonin for Reconstruction of Postoperative Bone Tumor (2023-01-05)
期刊:ACS Applied Materials & Interfaces
影响因子 :9.5
引用产品: U-2 OS [U2OS] 细胞 , MG-63 细胞 , B16 细胞
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High-contrast tumor imaging via a de novo designed aminopeptidase N targeted fluorogenic probe (2023-12-04)
期刊:SENSORS AND ACTUATORS B-CHEMICAL
影响因子 :8.4
引用产品: 4T1 细胞 , B16 细胞 , A549 [A-549] 细胞 , NCI-H1299 细胞 , HL-7702 [L-02; LO2] 细胞 , BEAS-2B 细胞 , Hep G2 细胞 , Malme-3M 细胞
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In situ administration of temperature-sensitive hydrogel composite loading paclitaxel microspheres and cisplatin for the treatment of melanoma (2023-02-10)
期刊:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2023.114380
影响因子 :7.5
引用产品: B16 细胞
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Study of the antiaging effects of bird's nest peptide based on biochemical, cellular, and animal models (2023-03-05)
期刊:Journal of Functional Foods
影响因子 :5.6
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Peptide OA-VI12 restrains melanogenesis in B16 cells and C57B/6 mouse ear skin via the miR-122-5p/Mitf/Tyr axis (2023-10-04)
期刊:AMINO ACIDS
DOI:10.1007/s00726-023-03341-x
影响因子 :3.5
引用产品: B16 细胞
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Extracellular matrix modulating enzyme functionalized biomimetic Au nanoplatform-mediated enhanced tumor penetration and synergistic antitumor therapy for pancreatic cancer (2022-12-10)
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-022-01738-6
影响因子 :9.4
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Retraction of “Label-Free Isolation of Low-Adhesion Cells with Stem Properties for Cancer Stem Cell-Specific Drug Evaluation” (2022-09-19)
期刊:ANALYTICAL CHEMISTRY
DOI:10.1021/acs.analchem.2c00444
影响因子 :8.0
引用产品: MCF7 [MCF-7] 细胞 , MDA-MB-231 细胞 , SUM159PT 细胞 , B16 细胞 , MDA-MB-435 细胞 , SK-BR-3 [SKBR3] 细胞 , McCoy's 5A 培养基
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Promotion of Melanoma Cell Proliferation by Cyclic Straining through Regulatory Morphogenesis (2022-10-06)
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 :6.2
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In Situ Self-Assembly Nanomicelle Microneedles for Enhanced Photoimmunotherapy via Autophagy Regulation Strategy (2002-01-20)
期刊:ACS Nano
影响因子 :17.1
引用产品: B16 细胞
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Impact of particle size and pH on protein corona formation of solid lipid nanoparticles: A proof-of-concept study (2010-00-20)
期刊:Acta Pharmaceutica Sinica B
DOI:10.1016/j.apsb.2020.10.023
影响因子 :14.5
引用产品: B16 细胞 , RAW 264.7 细胞
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Dissolving microneedles integrated with pH-responsive micelles containing AIEgen with ultra-photostability for enhancing melanoma photothermal therapy (2009-00-20)
期刊:Biomaterials Science
影响因子 :6.6
引用产品: B16 细胞
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A Digital Controlled Pulse Generator for a Possible Tumor Therapy Combining Irreversible Electroporation With Nanosecond Pulse Stimulation (2004-00-20)
期刊:IEEE Transactions on Biomedical Circuits and Systems
DOI:10.1109/TBCAS.2020.2987376
影响因子 :5.1
引用产品: B16 细胞
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A colorimetric and near -infrared ratiometric fluorescent probe for the determination of endogenous tyrosinase activity based on cyanine aggregation (2009-09-20)
期刊:Analyst
影响因子 :4.2
引用产品: Hela 细胞 , MCF7 [MCF-7] 细胞 , B16 细胞 , PUMC-HUVEC-T1
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