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产品概述
| 名称 | T84 (人结肠腺癌肺转移细胞) (STR鉴定正确) |
| 别称 | T-84; T 84 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
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| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 1.该细胞呈高度聚集生长，细胞聚集会导致在培养过程中部分细胞死亡，进而出现漂浮细胞和细胞碎片，同时该细胞代谢较快，需及时换液。2.细胞培养过程中会有漂浮的细胞，特别是传代第二天会比较明显，2-3天换液一次后即可贴壁生长。 |
| 背景描述 | T84细胞是从一位72岁男性结肠癌患者的肺转移灶建立的可移植人类癌细胞株；肿瘤组织皮下接种于BALB/c裸鼠，并连续进行移植。在裸鼠身上的移植过程中，细胞株始终保持结肠癌的原始组织性状。在无胸腺小鼠中传代23代后建立了T84细胞。T84细胞单层生长到饱和并在接触细胞间展现出紧密连接和桥粒，有很多关于多肽类激素和神经递质并维持定向电解质传输的受体。T84细胞展现了接触细胞中的紧密连接和桥粒，角蛋白免疫过氧化物酶染色阳性。 |
| 年龄（性别） | 男性；72岁 |
| 组织来源 | 器官：结肠；疾病：结直肠癌；取材转移灶：肺 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 结肠腺癌细胞 |
| 生物安全等级 | BSL-1 |
| 致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
| 受体表达情况 | neurotransmitter, expressed;peptide hormone, expressed |
| 基因表达情况 | carcinoembryonic antigen (CEA), 600ng/mL per 1×10^6 cells per 10 days; keratin |
| 保藏机构 | ATCC; CCL-248 ECACC; 88021101 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10 D2S1338 23,26 D3S1358 16,19 D5S818 12 D7S820 8,10 D8S1179 15 D13S317 9 D16S539 10,11 D18S51 17 D19S433 13 D21S11 31 FGA 24 PentaD 9 PentaE 14 TH01 6,9 TPOX 8,11 vWA 17,18 D6S1043 19,20 D12S391 19,21 D2S441 10,14 -
STR鉴定图
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参考文献
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Circ-METTL15 contributes to the proliferation, metastasis, immune escape and restrains apoptosis in lung cancer by regulating miR-1299/PDL1 axis (2011-01-20)
作者：Rui Zhang:1.Department of Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, China. ; Liang Shang:1.Department of Pharmacy, Jilin Provincial Cancer Hospital, Changchun, China. ; Jinniang Nan:1.Department of Clinical Medicine, Jiangxi Health Vocational College of China, Nanchang, China. ; Kai Niu:1.Department of Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, China. ; Jixin Dai:1.Department of Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, China. ; Xintian Jin:1.Department of Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, China. ; Xianghua Zhang:1.Department of Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, China. ;
期刊：Circ-METTL15 contributes to the proliferation, metastasis, immune escape and restrains apoptosis in lung cancer by regulating miR-1299/PDL1 axis
DOI：10.1080/08916934.2021.2001801
影响因子 ：3.5
引用产品： T84 细胞 , NCI-H1650 细胞 , NCI-H1299 细胞 , A549 [A-549] 细胞 , RPMI-1640 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100×
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miR-145-5p restrained cell growth, invasion, migration and tumorigenesis via modulating RHBDD1 in colorectal cancer via the EGFR-associated signaling pathway (2011-09-20)
作者：Ying Niu:1.Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, PR China. ; Jinping Zhang:1.Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, PR China. ; Yalin Tong:1.Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, PR China. ; Jiansheng Li:1.Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, PR China. ; Bingrong Liu:1.Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, PR China. Electronic address: bingrongcn@163.com. ;
期刊：miR-145-5p restrained cell growth, invasion, migration and tumorigenesis via modulating RHBDD1 in colorectal cancer via the EGFR-associated signaling pathway
DOI：10.1016/j.biocel.2019.105641
影响因子 ：4.0
引用产品： Caco-2 细胞 , DLD-1 细胞 , LoVo 细胞 , SW480 [SW-480] 细胞 , T84 细胞
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![SW 780 [SW-780, SW780] (人膀胱移行细胞癌) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0449-1.jpg)
