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产品概述
名称 | NCI-H1650 (人非小细胞肺癌细胞) (STR鉴定正确) |
别称 | H1650; H-1650; H1650_CO; NCIH1650 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
背景描述 | NCI-H1650细胞是于1987年从一名27岁白人男性(10年烟龄)支气管肺泡癌患者的胸腔积液中分离得到的。 |
年龄(性别) | 男性;27岁 |
组织来源 | 肺;腺癌;细支气管及肺泡癌;转移灶:胸水 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~42 hours |
保藏机构 | ATCC; CRL-5883 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11 D2S1338 19 D3S1358 18 D5S818 11 D7S820 8,9 D8S1179 12 D13S317 11 D16S539 11,12 D18S51 10 D19S433 15 D21S11 30 FGA 20 PentaD 8 PentaE 12 TH01 9.3 TPOX 11 vWA 18 D6S1043 13 D12S391 22 D2S441 10 -
STR鉴定图
参考文献
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ORC6 acts as an effective prognostic predictor for non‑small cell lung cancer and is closely associated with tumor progression (2024-01-11)
作者:Letian Chen:1.Department of Pulmonary and Critical Care Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China. ; Dongdong Zhang:1.Department of General Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China. ; Yujuan Chen:1.Department of Pulmonary and Critical Care Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China. ; Huilan Zhu:1.Department of Pulmonary and Critical Care Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China. ; Zhipeng Liu:1.Department of Pulmonary and Critical Care Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China. ; Zhiping Yu:1.Department of Pulmonary and Critical Care Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China. ; Junping Xie:1.Department of Pulmonary and Critic
期刊:ORC6 acts as an effective prognostic predictor for non‑small cell lung cancer and is closely associated with tumor progression
影响因子 :2.9
引用产品: A549 [A-549] 细胞 , PC-9 细胞 , NCI-H1299 细胞 , NCI-H1650 细胞 , BEAS-2B 细胞
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Identification of a necroptosis-related gene signature for making clinical predictions of the survival of patients with lung adenocarcinoma (2024-01-08)
作者:Xiaoping Zhou:1.Department of Laboratory Medicine, Clinical Medical College and the First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, China. ; Ming Zhao:1.Department of Gastroenterology, Clinical Medical College and the First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, China. ; Yingzi Fan:1.Department of Laboratory Medicine, Clinical Medical College and the First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, China. ; Ying Xu:1.Department of Laboratory Medicine, Clinical Medical College and the First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, China. ;
期刊:Identification of a necroptosis-related gene signature for making clinical predictions of the survival of patients with lung adenocarcinoma
影响因子 :2.7
引用产品: BEAS-2B 细胞 , A549 [A-549] 细胞 , NCI-H1650 细胞
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Effect of circ_0000009 on lung adenocarcinoma progression by regulating PDZD2 in a ceRNA- and RBP- dependent manner (2023-06-10)
作者:Tan Tan:1.Department of Thoracic Surgery, Geriatric Hospital of Nanjing Medical University, Nanjing, China. ; Mingming Ma:1.Department of Respiratory, the First People's Hospital of Xining, Xining, China. ; Shigui Xing:1.Department of Thoracic Surgery, Nanjing Gaochun People's Hospital, Nanjing, China. Electronic address: shigui_xing@163.com. ;
期刊:Effect of circ_0000009 on lung adenocarcinoma progression by regulating PDZD2 in a ceRNA- and RBP- dependent manner
DOI:10.1016/j.gene.2023.147555
影响因子 :3.5
引用产品: BEAS-2B 细胞 , A549 [A-549] 细胞 , NCI-H1650 细胞 , NCI-H1299 细胞
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Budding uninhibited by benzimidazoles 1 overexpression is associated with poor prognosis and malignant phenotype: A promising therapeutic target for lung adenocarcinoma (2023-02-24)
作者:Rui Chen:1.Graduate School, Medical College of Nanchang University, Nanchang, China. ; 2.Jiangxi Key Laboratory of Translational Research for Cancer, Jiangxi Clinical Research Center for Cancer, Jiangxi Cancer Hospital, The Second Affiliated Hospital of Nanchang Medical College, Nanchang, China. ; Zhiping Wang:1.Fujian Medical University, Fuzhou, China. ; Tianzhu Lu:1.Jiangxi Key Laboratory of Translational Research for Cancer, Jiangxi Clinical Research Center for Cancer, Jiangxi Cancer Hospital, The Second Affiliated Hospital of Nanchang Medical College, Nanchang, China. ; Yuzhen Liu:1.Graduate School, Medical College of Nanchang University, Nanchang, China. ; 2.Jiangxi Key Laboratory of Translational Research for Cancer, Jiangxi Clinical Research Center for Cancer, Jiangxi Cancer Hospital, The Second Affiliated Hospital of Nanchang Medical College, Nanchang, China. ; Yulong Ji:1.Jiangxi Key Laboratory of Translational Research for Cancer, Jiangxi Clinical Research Center for Canc
期刊:Budding uninhibited by benzimidazoles 1 overexpression is associated with poor prognosis and malignant phenotype: A promising therapeutic target for lung adenocarcinoma
影响因子 :2.9
引用产品: MRC-5 细胞 , MRC-5细胞专用培养基 , NCI-H1299 细胞 , NCI-H1975 细胞 , A549 [A-549] 细胞 , NCI-H1650 细胞 , PC-9 细胞
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RPL11 promotes non-small cell lung cancer cell proliferation by regulating endoplasmic reticulum stress and cell autophagy (2023-03-03)
作者:Jie Chen:1.Medical Research and Experimental Center, Medical College, Yan'an University, 716000, Yan'an, People's Republic of China. ; 2.Yan'an Key Laboratory of Chronic Disease Prevention and Research, 716000, Yan'an, Shaanxi Province, China. ; Changda Lei:1.Department of Gastroenterology, Ninth Hospital of Xi 'an, 710054, Xi'an, Shaanxi Province, China. ; Huahua Zhang:1.Medical Research and Experimental Center, Medical College, Yan'an University, 716000, Yan'an, People's Republic of China. ; 2.Yan'an Key Laboratory of Chronic Disease Prevention and Research, 716000, Yan'an, Shaanxi Province, China. ; Xiaoyong Huang:1.Medical Research and Experimental Center, Medical College, Yan'an University, 716000, Yan'an, People's Republic of China. ; 2.Yan'an Key Laboratory of Chronic Disease Prevention and Research, 716000, Yan'an, Shaanxi Province, China. ; Yang Yang:1.Medical Research and Experimental Center, Medical College, Yan'an University, 716000, Yan'an, People's Republic of China.
期刊:RPL11 promotes non-small cell lung cancer cell proliferation by regulating endoplasmic reticulum stress and cell autophagy
DOI:10.1186/s12860-023-00469-2
影响因子 :2.8
引用产品: NCI-H1299 细胞 , NCI-H1650 细胞
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N6-methyladenosine-induced SVIL antisense RNA 1 restrains lung adenocarcinoma cell proliferation by destabilizing E2F1 (2001-02-20)
作者:Zedong Hu:1.Second Department of Thoracic Surgery, Anhui Chest Hospital, Hefei, China. ; Liang Zhu:1.Second Department of Thoracic Surgery, Anhui Chest Hospital, Hefei, China. ; Yilin Zhang:1.Second Department of Thoracic Surgery, Anhui Chest Hospital, Hefei, China. ; Bing Chen:1.Second Department of Thoracic Surgery, Anhui Chest Hospital, Hefei, China. ;
期刊:N6-methyladenosine-induced SVIL antisense RNA 1 restrains lung adenocarcinoma cell proliferation by destabilizing E2F1
DOI:10.1080/21655979.2022.2025697
影响因子 :4.9
引用产品: A549 [A-549] 细胞 , NCI-H1650 细胞
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Hsa_circ_0017639 regulates cisplatin resistance and tumor growth via acting as a miR-1296-5p molecular sponge and modulating sine oculis homeobox 1 expression in non-small cell lung cancer (2003-02-20)
作者:Feiyun Chang:1.Department of Thoracic Surgery, Shanxi Province Cancer Hospital, Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences, Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, Shanxi, China. ; Jiali Li:1.Department of Thoracic Surgery, Shanxi Province Cancer Hospital, Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences, Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, Shanxi, China. ; Quan Sun:1.Department of Thoracic Surgery, Shanxi Province Cancer Hospital, Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences, Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, Shanxi, China. ; Shuqing Wei:1.Department of Thoracic Surgery, Shanxi Province Cancer Hospital, Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences, Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, Shanxi, China. ; Yongming Song:1.D
期刊:Hsa_circ_0017639 regulates cisplatin resistance and tumor growth via acting as a miR-1296-5p molecular sponge and modulating sine oculis homeobox 1 expression in non-small cell lung cancer
DOI:10.1080/21655979.2022.2053810
影响因子 :4.9
引用产品: 293T [HEK-293T] 细胞 , NCI-H460 [H460] 细胞 , NCI-H1650 细胞 , A549 [A-549] 细胞 , NCI-H1299 细胞 , HBE135-E6E7 细胞
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Identification of a Novel circ_0010235/miR-1249-3p/HOXA13 Axis in Lung Adenocarcinoma (2001-02-20)
作者:Jiancheng Liu:1.Department of Thoracic Surgery, Shanxi Provincial Cancer Hospital, No.3 Worker's New Street, Xinhualing District, Taiyuan, 030001, Shanxi Province, China. ; Zejun Fu:1.Department of Thoracic Surgery, Shanxi Provincial Cancer Hospital, No.3 Worker's New Street, Xinhualing District, Taiyuan, 030001, Shanxi Province, China. fuze_jun@126.com. ;
期刊:Identification of a Novel circ_0010235/miR-1249-3p/HOXA13 Axis in Lung Adenocarcinoma
DOI:10.1007/s10528-021-10181-5
影响因子 :2.4
引用产品: 16HBE 细胞 , NCI-H1650 细胞 , NCI-H1299 细胞
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Curcumin suppresses the malignancy of non-small cell lung cancer by modulating the circ-PRKCA/miR-384/ITGB1 pathway (2003-01-20)
作者:Xiaoqing Xu:1.Department of Clinical Oncology, The Affiliated Hospital of Shandong University of TCM, Jinan, Shandong, China. ; Xinyue Zhang:1.Department of Respiratory Diseases, The First Clinical Medical College of Shandong University of TCM, Jinan, Shandong, China. ; Yang Zhang:1.Department of Respiratory Diseases, The Affiliated Hospital of Shandong University of TCM, Jinan, Shandong, China. ; Zhipeng Wang:1.Department of Clinical Oncology, The Affiliated Hospital of Shandong University of TCM, Jinan, Shandong, China. Electronic address: jnxuxiaoqing@126.com. ;
期刊:Curcumin suppresses the malignancy of non-small cell lung cancer by modulating the circ-PRKCA/miR-384/ITGB1 pathway
DOI:10.1016/j.biopha.2021.111439
影响因子 :7.5
引用产品: 16HBE 细胞 , NCI-H1650 细胞 , NCI-H1299 细胞 , NCI-H460 [H460] 细胞 , A549 [A-549] 细胞 , 293T [HEK-293T] 细胞 , RPMI-1640 培养基 , 特级胎牛血清
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YBX1 regulated by Runx3-miR-148a-3p axis facilitates non-small-cell lung cancer progression (2005-01-20)
作者:Hongbo Su:1.Department of Pathology, First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, PR China. ; Guanzhi Fan:1.Department of Pathology, Shengjing Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, PR China. ; Jin Huang:1.Department of Radiotherapy, First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, PR China. ; Xueshan Qiu:1.Department of Pathology, First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, PR China. Electronic address: xsqiu@cmu.edu.cn. ;
期刊:YBX1 regulated by Runx3-miR-148a-3p axis facilitates non-small-cell lung cancer progression
DOI:10.1016/j.cellsig.2021.110049
影响因子 :4.8
引用产品: BEAS-2B , HCC827 细胞 , NCI-H1650 细胞 , NCI-H1299 细胞 , NCI-H358 细胞 , A549 [A-549] 细胞 , RPMI-1640 培养基 , 人支气管上皮细胞完全培养基
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Comprehensive analysis of an immune infiltrate-related competitive endogenous RNA network reveals potential prognostic biomarkers for non-small cell lung cancer (2012-01-20)
作者:Cai-Zhi Yang:1.The First School of Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China. ; Lei-Hao Hu:1.The First School of Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China. ; Zhong-Yu Huang:1.Integrated Chinese and Western Medicine Postdoctoral research station, Jinan University, Guangzhou, China. ; Li Deng:1.Department of Radiotherapy, Guangdong Second Provincial General Hospital, Guangzhou, China. ; Wei Guo:1.Department of Oncology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China. ; Shan Liu:1.The First School of Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China. ; Xi Xiao:1.Postdoctoral Research Station, Guangzhou University of Chinese Medicine, Guangzhou, China. ; Hong-Xing Yang:1.Postdoctoral Research Station, Guangzhou University of Chinese Medicine, Guangzhou, China. ; Jie-Tao Lin:1.Department of Oncology, the First Affiliated Hospital of Guangzhou University of Chinese Me
期刊:Comprehensive analysis of an immune infiltrate-related competitive endogenous RNA network reveals potential prognostic biomarkers for non-small cell lung cancer
DOI:10.1371/journal.pone.0260720
影响因子 :3.7
引用产品: 青霉素-链霉素溶液 (双抗),100× , NCI-H1650 细胞 , NCI-H1975 细胞 , DMEM高糖 培养基
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Circ-METTL15 contributes to the proliferation, metastasis, immune escape and restrains apoptosis in lung cancer by regulating miR-1299/PDL1 axis (2011-01-20)
作者:Rui Zhang:1.Department of Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, China. ; Liang Shang:1.Department of Pharmacy, Jilin Provincial Cancer Hospital, Changchun, China. ; Jinniang Nan:1.Department of Clinical Medicine, Jiangxi Health Vocational College of China, Nanchang, China. ; Kai Niu:1.Department of Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, China. ; Jixin Dai:1.Department of Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, China. ; Xintian Jin:1.Department of Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, China. ; Xianghua Zhang:1.Department of Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, China. ;
期刊:Circ-METTL15 contributes to the proliferation, metastasis, immune escape and restrains apoptosis in lung cancer by regulating miR-1299/PDL1 axis
DOI:10.1080/08916934.2021.2001801
影响因子 :3.5
引用产品: T84 细胞 , NCI-H1650 细胞 , NCI-H1299 细胞 , A549 [A-549] 细胞 , RPMI-1640 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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Overexpression of Human Aspartyl (Asparaginyl) β-hydroxylase in NSCLC: Its Diagnostic Value by Means of Exosomes of Bronchoalveolar Lavage (2011-01-20)
作者:Haohao Zhu:1.Department of Pathology, The First Affiliated Hospital of Nanchang University. ; 2.Departments of Pathology. ; Huai Liu:1.Jiujiang University Affiliated Hospital, Jiujiang, Jiangxi Province, China. ; Jianfeng Wen:1.Neurosurgery, The 908th Hospital of PLA Joint Logistic Support Force. ; Ting Yuan:1.Departments of Pathology. ; Guangyu Ren:1.Departments of Pathology. ; Yonqing Jiang:1.Jiujiang University Affiliated Hospital, Jiujiang, Jiangxi Province, China. ; Yujun Yuan:1.Jiujiang University Affiliated Hospital, Jiujiang, Jiangxi Province, China. ; Jinhong Mei:1.Department of Pathology, The First Affiliated Hospital of Nanchang University. ; 2.Jiangxi Huitai Biotech Ltd, Nanchang. ; Yuefei Yu:1.Jiangxi Huitai Biotech Ltd, Nanchang. ; Guorong Li:1.Department of Urology, North Hospital, CHU Saint-Etienne, Saint-Étienne, France. ;
期刊:Overexpression of Human Aspartyl (Asparaginyl) β-hydroxylase in NSCLC: Its Diagnostic Value by Means of Exosomes of Bronchoalveolar Lavage
DOI:10.1097/PAI.0000000000000963
影响因子 :1.6
引用产品: A549 [A-549] 细胞 , NCI-H1299 细胞 , NCI-H1650 细胞 , HCC827 细胞
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One-step determination of deletion mutation based on loop-mediated isothermal amplification (2012-00-20)
作者:Xiaonan Liu:1.College of Life Sciences, Northwest University, Xi'an, Shaanxi, 710069, China. ; Chao Zhang:1.College of Life Sciences, Northwest University, Xi'an, Shaanxi, 710069, China. ; Sinong Zhang:1.College of Life Sciences, Northwest University, Xi'an, Shaanxi, 710069, China. ; Yu Cai:1.College of Life Sciences, Northwest University, Xi'an, Shaanxi, 710069, China. ; Kai Hua:1.College of Life Sciences, Northwest University, Xi'an, Shaanxi, 710069, China; Shaanxi Provincial Engineering Research Center for Nano-Biomedical Detection, Xi'an, Shaanxi, 710077, China. ; Yali Cui:1.College of Life Sciences, Northwest University, Xi'an, Shaanxi, 710069, China; Shaanxi Provincial Engineering Research Center for Nano-Biomedical Detection, Xi'an, Shaanxi, 710077, China. Electronic address: yalicui@nwu.edu.cn. ;
期刊:One-step determination of deletion mutation based on loop-mediated isothermal amplification
影响因子 :2.9
引用产品: A549 [A-549] 细胞 , NCI-H1650 细胞
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EGFR mutation status in Tunisian non-small-cell lung cancer patients evaluated by mutation-specific immunohistochemistry (2008-08-20)
作者:Zohra Mraihi:1.Genetic Department, Faculté de Médecine de Tunis, Université de Tunis El Manar, Tunis, Tunisia. zohra_mraihi@yahoo.fr. ; Jihen Ben Amar:1.Pulmonary Department, EPS Charles Nicolle, Faculté de Médecine de Tunis, Université de Tunis El Manar, Tunis, Tunisia. ; Hend Bouacha:1.Pulmonary Department, EPS Charles Nicolle, Faculté de Médecine de Tunis, Université de Tunis El Manar, Tunis, Tunisia. ; Soumaya Rammeh:1.Pathological Anatomy and Cytology Department, EPS Charles Nicolle, Faculté de Médecine de Tunis, Université de Tunis El Manar, Tunis, Tunisia. ; Lamia Hila:1.Genetic Department, Faculté de Médecine de Tunis, Université de Tunis El Manar, Tunis, Tunisia. lamia_hila@yahoo.fr. ;
期刊:EGFR mutation status in Tunisian non-small-cell lung cancer patients evaluated by mutation-specific immunohistochemistry
影响因子 :3.1
引用产品: A549 [A-549] 细胞 , NCI-H1650 细胞 , NCI-H1975 细胞
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