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产品概述
名称 | NCI-H1650 (人非小细胞肺癌细胞) (STR鉴定正确) |
别称 | H1650; H-1650; H1650_CO; NCIH1650 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
背景描述 | NCI-H1650细胞是于1987年从一名27岁白人男性(10年烟龄)支气管肺泡癌患者的胸腔积液中分离得到的。 |
年龄(性别) | 男性;27岁 |
组织来源 | 肺;腺癌;细支气管及肺泡癌;转移灶:胸水 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~42 hours |
保藏机构 | ATCC; CRL-5883 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11 D2S1338 19 D3S1358 18 D5S818 11 D7S820 8,9 D8S1179 12 D13S317 11 D16S539 11,12 D18S51 10 D19S433 15 D21S11 30 FGA 20 PentaD 8 PentaE 12 TH01 9.3 TPOX 11 vWA 18 D6S1043 13 D12S391 22 D2S441 10 -
STR鉴定图
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参考文献
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Super-Resolution Fluorescence Imaging Reveals the Mechanism of NRP1 Clustering on Non-Small-Cell Lung Cancer Membranes (2025-01-22)
期刊:ANALYTICAL CHEMISTRY
DOI:10.1021/acs.analchem.4c05675
影响因子 :6.7
引用产品: NCI-H1650 细胞 , NCI-H1975 细胞 , HCC827 细胞
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FAM64A regulates the malignant phenotype and tumor microenvironment of non-small cell lung cancer by activating the JAK/STAT3/PDL1 axis (2025-02-24)
期刊:JOURNAL OF MOLECULAR HISTOLOGY
DOI:10.1007/s10735-024-10339-6
影响因子 :2.9
引用产品: A549 [A-549] 细胞 , NCI-H1299 细胞 , NCI-H460 [H460] 细胞 , NCI-H1650 细胞 , BEAS-2B 细胞 , Ham's F-12K 培养基 , RPMI-1640 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液(双抗),100×
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Brain Metastasis from EGFR-Mutated Non-Small Cell Lung Cancer: Secretion of IL11 from Astrocytes Up-Regulates PDL1 and Promotes Immune Escape (2024-05-02)
期刊:Advanced Science
影响因子 :15.1
引用产品: LLC [LL/2; LLC1] 细胞 , NCI-H1650 细胞
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N6-methyladenosine-modified SRPK1 promotes aerobic glycolysis of lung adenocarcinoma via PKM splicing (2024-08-02)
期刊:CELLULAR & MOLECULAR BIOLOGY LETTERS
DOI:10.1186/s11658-024-00622-5
影响因子 :9.2
引用产品: BEAS-2B 细胞 , NCI-H226 [H226] 细胞 , HCC827 细胞 , NCI-H1299 细胞 , RPMI-1640 培养基 , A549 [A-549] 细胞 , PC-9 细胞 , DMEM高糖 培养基 , 293T [HEK-293T] 细胞 , NCI-H1650 细胞 , NCI-H1975 细胞
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NOP2 facilitates EZH2-mediated epithelial–mesenchymal transition by enhancing EZH2 mRNA stability via m5C methylation in lung cancer progression (2024-07-16)
期刊:Cell Death & Disease
DOI:10.1038/s41419-024-06899-w
影响因子 :8.1
引用产品: A549 [A-549] 细胞 , NCI-H358 细胞 , NCI-H1650 细胞 , NCI-H1299 细胞
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ORC6 acts as an effective prognostic predictor for non?small cell lung cancer and is closely associated with tumor progression (2024-01-11)
期刊:Oncology Letters
影响因子 :2.9
引用产品: A549 [A-549] 细胞 , PC-9 细胞 , NCI-H1299 细胞 , NCI-H1650 细胞 , BEAS-2B 细胞
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Identification of a necroptosis-related gene signature for making clinical predictions of the survival of patients with lung adenocarcinoma (2024-01-08)
期刊:PeerJ
影响因子 :2.7
引用产品: BEAS-2B 细胞 , A549 [A-549] 细胞 , NCI-H1650 细胞
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Curcumin Inhibits the Progression of Non-small Cell Lung Cancer by Regulating DMRT3/SLC7A11 Axis (2024-05-14)
期刊:MOLECULAR BIOTECHNOLOGY
DOI:10.1007/s12033-024-01166-x
影响因子 :2.6
引用产品: 人脐静脉内皮细胞 , 16HBE 细胞 , NCI-H1650 细胞
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USP8-mediated PTK7 promotes PIK3CB-related pathway to accelerate the malignant progression of non-small cell lung cancer (2024-11-17)
期刊:Thoracic Cancer
影响因子 :2.3
引用产品: NCI-H1299 细胞 , THP-1 细胞 , HBE 细胞 , A549 [A-549] 细胞 , HCC827 细胞 , NCI-H1650 细胞
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MECOM: a bioinformatics and experimentally identified marker for the diagnosis and prognosis of lung adenocarcinoma (2024-03-05)
期刊:Biomarkers in Medicine
影响因子 :1.9
引用产品: A549 [A-549] 细胞 , NCI-H1650 细胞 , BEAS-2B 细胞
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circPTP4A2 knockdown suppresses NSCLC progression via regulating proliferation and activating anti-tumor immunity (2024-07-16)
期刊:Journal of Cardiothoracic Surgery
DOI:10.1186/s13019-024-02964-9
影响因子 :1.5
引用产品: BEAS-2B 细胞 , NCI-H1650 细胞 , Calu-3 细胞 , A549 [A-549] 细胞 , NCI-H1299 细胞
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Effect of circ_0000009 on lung adenocarcinoma progression by regulating PDZD2 in a ceRNA- and RBP- dependent manner (2023-06-10)
期刊:GENE
DOI:10.1016/j.gene.2023.147555
影响因子 :3.5
引用产品: BEAS-2B 细胞 , A549 [A-549] 细胞 , NCI-H1650 细胞 , NCI-H1299 细胞
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GIMAP7 inhibits epithelial-mesenchymal transition and glycolysis in lung adenocarcinoma cells via regulating the Smo/AMPK signaling pathway (2023-12-27)
期刊:Thoracic Cancer
影响因子 :2.9
引用产品: NCI-H1650 细胞 , A549 [A-549] 细胞 , NCI-H1299 细胞
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Budding uninhibited by benzimidazoles 1 overexpression is associated with poor prognosis and malignant phenotype: A promising therapeutic target for lung adenocarcinoma (2023-02-24)
期刊:Thoracic Cancer
影响因子 :2.9
引用产品: MRC-5 细胞 , MRC-5细胞专用培养基 , NCI-H1299 细胞 , NCI-H1975 细胞 , A549 [A-549] 细胞 , NCI-H1650 细胞 , PC-9 细胞
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RPL11 promotes non-small cell lung cancer cell proliferation by regulating endoplasmic reticulum stress and cell autophagy (2023-03-03)
期刊:BMC Molecular and Cell Biology
DOI:10.1186/s12860-023-00469-2
影响因子 :2.8
引用产品: NCI-H1299 细胞 , NCI-H1650 细胞
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N6-methyladenosine-induced SVIL antisense RNA 1 restrains lung adenocarcinoma cell proliferation by destabilizing E2F1 (2022-01-22)
期刊:Bioengineered
DOI:10.1080/21655979.2022.2025697
影响因子 :4.9
引用产品: A549 [A-549] 细胞 , NCI-H1650 细胞
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Hsa_circ_0017639 regulates cisplatin resistance and tumor growth via acting as a miR-1296-5p molecular sponge and modulating sine oculis homeobox 1 expression in non-small cell lung cancer (2022-03-28)
期刊:Bioengineered
DOI:10.1080/21655979.2022.2053810
影响因子 :4.9
引用产品: 293T [HEK-293T] 细胞 , NCI-H460 [H460] 细胞 , NCI-H1650 细胞 , A549 [A-549] 细胞 , NCI-H1299 细胞 , HBE135-E6E7 细胞
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Identification of a Novel circ_0010235/miR-1249-3p/HOXA13 Axis in Lung Adenocarcinoma (2022-01-25)
期刊:Biochemical Genetics
DOI:10.1007/s10528-021-10181-5
影响因子 :2.4
引用产品: 16HBE 细胞 , NCI-H1650 细胞 , NCI-H1299 细胞
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Curcumin suppresses the malignancy of non-small cell lung cancer by modulating the circ-PRKCA/miR-384/ITGB1 pathway (2021-03-06)
期刊:Biomedicine & Pharmacotherapy
DOI:10.1016/j.biopha.2021.111439
影响因子 :7.5
引用产品: 16HBE 细胞 , NCI-H1650 细胞 , NCI-H1299 细胞 , NCI-H460 [H460] 细胞 , A549 [A-549] 细胞 , 293T [HEK-293T] 细胞 , RPMI-1640 培养基 , 特级胎牛血清
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YBX1 regulated by Runx3-miR-148a-3p axis facilitates non-small-cell lung cancer progression (2021-05-31)
期刊:Cellular Signalling
DOI:10.1016/j.cellsig.2021.110049
影响因子 :4.8
引用产品: BEAS-2B , HCC827 细胞 , NCI-H1650 细胞 , NCI-H1299 细胞 , NCI-H358 细胞 , A549 [A-549] 细胞 , RPMI-1640 培养基 , 人支气管上皮细胞完全培养基
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Comprehensive analysis of an immune infiltrate-related competitive endogenous RNA network reveals potential prognostic biomarkers for non-small cell lung cancer (2021-12-02)
期刊:PLoS One
DOI:10.1371/journal.pone.0260720
影响因子 :3.7
引用产品: 青霉素-链霉素溶液 (双抗),100× , NCI-H1650 细胞 , NCI-H1975 细胞 , DMEM高糖 培养基
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Circ-METTL15 contributes to the proliferation, metastasis, immune escape and restrains apoptosis in lung cancer by regulating miR-1299/PDL1 axis (2021-11-19)
期刊:Autoimmunity
DOI:10.1080/08916934.2021.2001801
影响因子 :3.5
引用产品: T84 细胞 , NCI-H1650 细胞 , NCI-H1299 细胞 , A549 [A-549] 细胞 , RPMI-1640 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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Overexpression of Human Aspartyl (Asparaginyl) β-hydroxylase in NSCLC: Its Diagnostic Value by Means of Exosomes of Bronchoalveolar Lavage (2021-11-01)
期刊:Applied Immunohistochemistry & Molecular Morphology
DOI:10.1097/PAI.0000000000000963
影响因子 :1.6
引用产品: A549 [A-549] 细胞 , NCI-H1299 细胞 , NCI-H1650 细胞 , HCC827 细胞
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One-step determination of deletion mutation based on loop-mediated isothermal amplification (2020-12-19)
期刊:Analytical Biochemistry
影响因子 :2.9
引用产品: A549 [A-549] 细胞 , NCI-H1650 细胞
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EGFR mutation status in Tunisian non-small-cell lung cancer patients evaluated by mutation-specific immunohistochemistry (2018-08-09)
期刊:BMC Pulmonary Medicine
影响因子 :3.1
引用产品: A549 [A-549] 细胞 , NCI-H1650 细胞 , NCI-H1975 细胞
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