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产品概述
名称 | DLD-1 (人结直肠腺癌上皮细胞) (STR鉴定正确) |
别称 | DLD 1; DLD1; CoCL3 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:8 |
推荐换液频率 | 2-3次/周 |
背景描述 | DLD-1细胞是由D·L·Dexter和其同事于1977-1979年分离的两株结直肠腺癌细胞株中的一株。在ATCC和其它地方进行的DNA指纹鉴定和染色体组型分析表明DLD-1细胞与HCT-15细胞相似,说明这两者是来自同一个人的不同克隆。DLD-1细胞和HCT-15细胞的遗传起源可通过DNA指纹鉴定证实,但染色体组型分析显示它们缺乏染色体标记一致改变或数目上一致改变。DLD-1细胞的CSAp阴性(CSAp-),p53抗原表达呈阳性(p53抗原产生了一个C→T点突变导致241位的Ser→Phe)。DLD-1细胞角蛋白免疫过氧化物酶染色阳性,癌基因c-myc、K-ras、H-ras、N-ras、myb、sis和fos的表达呈阳性,癌基因N-myc的表达未做检测。DLD-1细胞表达肿瘤特异性核基质蛋白CC-2、CC-3、CC-4、CC-5和CC-6。1979年提交到ATCC的DLD-1细胞代数不明且污染了支原体,其后经过12周多种抗生素联合培养处理,处理之后每周用Hoechst染色和标准培养法检测。其后连续11个月不加抗生素培养,DLD-1细胞所有的检测呈阴性。 |
年龄(性别) | 男性;成人 |
组织来源 | 结直肠腺癌上皮细胞 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肠癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~24-48 hours |
致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
抗原表达情况 | Blood Type O.The cells are weakly positive for keratins and vimentin.The cells are positive for keratin by immunoperoxidase staining.DLD-1 cells are positive for p53 antigen expression (the p53 antigen produced has a C -> T |
基因表达情况 | carcinoembryonic antigen (CEA) 0.5 ng/10^6 cells/10 days; colon antigen 3. |
保藏机构 | ATCC; CCL-221 BCRC; 60132 DSMZ; ACC-278 ECACC; 90102540 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 17,25 D3S1358 17 D5S818 13 D7S820 10,12 D8S1179 15 D13S317 8,11 D16S539 12,13 D18S51 11,17 D19S433 14,16 D21S11 29,32.2 FGA 22 PentaD 9,14 PentaE 7,14 TH01 7,9.3 TPOX 8,11 vWA 18,19 D6S1043 11,13 D12S391 19,22 D2S441 13,15 -
STR鉴定图
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参考文献
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Silencing of SIRPα enhances the antitumor efficacy of CAR-M in solid tumors (2024-10-08)
期刊:Cellular & Molecular Immunology
DOI:10.1038/s41423-024-01220-3
影响因子 :21.8
引用产品: THP-1 细胞 , SK-OV-3 细胞 , DLD-1 细胞 , AsPC-1 细胞 , MC38 细胞 , B16 细胞 , SK-BR-3 [SKBR3] 细胞 , ID8 细胞
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Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis (2024-01-17)
期刊:Acta Pharmaceutica Sinica B
DOI:10.1016/j.apsb.2024.01.006
影响因子 :14.5
引用产品: 特级胎牛血清 , LoVo 细胞 , HT-29 细胞 , HCT 116 细胞 , SW620 细胞 , RKO 细胞 , DLD-1 细胞 , HCT-8 [HRT-18] 细胞
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CircSEC24B activates autophagy and induces chemoresistance of colorectal cancer via OTUB1-mediated deubiquitination of SRPX2 (2024-09-27)
期刊:Cell Death & Disease
DOI:10.1038/s41419-024-07057-y
影响因子 :8.1
引用产品: LoVo 细胞 , SW620 细胞 , HCT 116 细胞 , 293T [HEK-293T] 细胞 , DLD-1 细胞 , SW48 细胞 , NCM460 细胞
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VWA2 protein molecular mechanism predicts colorectal cancer: Promoting cell invasion and migration by inhibiting NK cell activation (2024-09-06)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2024.135394
影响因子 :7.7
引用产品: DLD-1 细胞 , HCT 116 细胞 , HT-29 细胞 , LoVo 细胞
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Norcantharidin Sensitizes Colorectal Cancer Cells to Radiotherapy via Reactive Oxygen Species–DRP1-Mediated Mitochondrial Damage (2024-03-14)
期刊:Antioxidants
影响因子 :7.0
引用产品: DLD-1 细胞
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DDR1 is identified as an immunotherapy target for microsatellite stable colon cancer by CRISPR screening (2024-11-07)
期刊:npj Precision Oncology
DOI:10.1038/s41698-024-00743-2
影响因子 :6.8
引用产品: HT-29 细胞 , CT26.WT 细胞 , DLD-1 细胞 , HCT 116 细胞
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METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1 (2024-01-24)
期刊:CANCER GENE THERAPY
DOI:10.1038/s41417-024-00731-5
影响因子 :6.4
引用产品: HCT 116 细胞 , DLD-1 细胞 , 293T [HEK-293T] 细胞
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SERPINB5 promotes colorectal cancer invasion and migration by promoting EMT and angiogenesis via the TNF-α/NF-κB pathway (2024-03-08)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2024.111759
影响因子 :5.6
引用产品: DLD-1 细胞 , SW480 [SW-480] 细胞 , NCM460 细胞
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N6-methyladenosine modification of B7-H3 mRNA promotes the development and progression of colorectal cancer (2024-01-18)
期刊:iScience
DOI:10.1016/j.isci.2024.108956
影响因子 :4.6
引用产品: HCT 116 细胞 , DLD-1 细胞
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NAT1 inhibits liver metastasis of colorectal cancer by regulating EMT and glycolysis (2024-06-24)
期刊:Aging-US
影响因子 :3.9
引用产品: LoVo 细胞 , DLD-1 细胞 , SW480 [SW-480] 细胞 , HCT 116 细胞
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Integrated Multi-omics Analyses Identify CDCA5 as a Novel Biomarker Associated with Alternative Splicing, Tumor Microenvironment, and Cell Proliferation in Colon Cancer Via Pan-cancer Analysis (2024-01-01)
期刊:Journal of Cancer
影响因子 :3.9
引用产品: DLD-1 细胞
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Piperine induces autophagy of colon cancer cells: Dual modulation of AKT/mTOR signaling pathway and ROS production (2024-07-02)
期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI:10.1016/j.bbrc.2024.150340
影响因子 :2.5
引用产品: HCT 116 细胞 , SW480 [SW-480] 细胞 , SW620 细胞 , DLD-1 细胞 , CT26.WT 细胞 , NCM460 细胞 , IEC-6 细胞
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WTAP-mediated m6A modification regulates NLRP3/Caspase-1/GSDMD to inhibit pyroptosis and exacerbate colorectal cancer (2024-10-29)
期刊:Biomarkers in Medicine
DOI:10.1080/17520363.2024.2416886
影响因子 :1.9
引用产品: DLD-1 细胞 , HCT 116 细胞 , SW480 [SW-480] 细胞
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Mollugin suppresses proliferation and drives ferroptosis of colorectal cancer cells through inhibition of insulin-like growth factor 2 mRNA binding protein 3/glutathione peroxidase 4 axis (2023-09-05)
期刊:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2023.115427
影响因子 :7.5
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Identification and validation of a novel prognosis model based on m5C-related long non-coding RNAs in colorectal cancer (2023-09-05)
期刊:Cancer Cell International
DOI:10.1186/s12935-023-03025-2
影响因子 :5.8
引用产品: NCM460 细胞 , SW620 细胞 , HCT 116 细胞 , SW480 [SW-480] 细胞 , DLD-1 细胞 , LoVo 细胞 , HT-29 细胞
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Copine 7 promotes colorectal cancer proliferation through PKM2 interaction and MAPK signaling pathway (2023-07-04)
期刊:Frontiers in Oncology
影响因子 :4.7
引用产品: 293 [HEK-293] 细胞 , HCT 116 细胞 , DLD-1 细胞 , McCoy's 5A 培养基 , 特级胎牛血清 , RPMI-1640 培养基 , DMEM高糖 培养基
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RNF114 facilitates the proliferation, stemness, and metastasis of colorectal cancer (2023-07-27)
期刊:PATHOLOGY RESEARCH AND PRACTICE
影响因子 :2.8
引用产品: DLD-1 细胞 , HT-29 细胞 , SW480 [SW-480] 细胞
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Acetyltransferase NAT10 regulates the Wnt/β-catenin signaling pathway to promote colorectal cancer progression via ac4C acetylation of KIF23 mRNA (2022-12-15)
期刊:JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI:10.1186/s13046-022-02551-7
影响因子 :12.7
引用产品: 293T [HEK-293T] 细胞 , NCM460 细胞 , SW480 [SW-480] 细胞 , DLD-1 细胞 , HT-29 细胞 , RKO 细胞 , HCT 116 细胞
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Critical role of guanylate binding protein 5 in tumor immune microenvironment and predictive value of immunotherapy response (2022-09-30)
期刊:Frontiers in Genetics
影响因子 :4.8
引用产品: DLD-1 细胞
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Lipofectamine 2000™ at transfection dose promotes EphA2 transcription in an HDAC4-dependent manner to reduce its cytotoxicity (2022-12-06)
期刊:Heliyon
DOI:10.1016/j.heliyon.2022.e12118
影响因子 :3.8
引用产品: Hep G2 细胞 , HuH-7 细胞 , 293T [HEK-293T] 细胞 , AGS 细胞 , DLD-1 细胞 , Hela 细胞 , NCI-H460 [H460] 细胞
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HSPA5 repressed ferroptosis to promote colorectal cancer development by maintaining GPX4 stability (2022-09-01)
期刊:NEOPLASMA
DOI:10.4149/neo_2022_220331N363
影响因子 :3.4
引用产品: DLD-1 细胞 , SW480 [SW-480] 细胞
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CAMK2N1 has a cancer-suppressive function in colorectal carcinoma via effects on the Wnt/β-catenin pathway (2022-08-15)
期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI:10.1016/j.bbrc.2022.08.036
影响因子 :3.3
引用产品: LS180 细胞 , SW480 [SW-480] 细胞 , HT-29 细胞 , HCT 116 细胞 , DLD-1 细胞
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Circ_KIAA1199 inhibits MSI1 degradation by targeting miR-34c-5p to drive the malignant cell behaviors and tumor growth of colorectal cancer (2022-01-01)
期刊:Anti-Cancer Drugs
DOI:10.1097/CAD.0000000000001164
影响因子 :2.3
引用产品: HCT 116 细胞 , DLD-1 细胞
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Autophagy Induction by Trichodermic Acid Attenuates Endoplasmic Reticulum Stress-Mediated Apoptosis in Colon Cancer Cells (2021-05-25)
期刊:International Journal Of Molecular Sciences
影响因子 :5.6
引用产品: HCT 116 细胞 , DLD-1 细胞
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Raltitrexed as a synergistic hyperthermia chemotherapy drug screened in patient-derived colorectal cancer organoids (2021-08-15)
期刊:Cancer Biology & Medicine
DOI:10.20892/j.issn.2095-3941.2020.0566
影响因子 :5.5
引用产品: SW620 细胞 , SW480 [SW-480] 细胞 , DLD-1 细胞 , COLO 205 细胞
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Cullin-4B promotes cell proliferation and invasion through inactivation of p53 signaling pathway in colorectal cancer (2021-06-08)
期刊:Pathology Research And Practice
影响因子 :2.8
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The combination of lactoferrin and linolenic acid inhibits colorectal tumor growth through activating AMPK/JNK-related apoptosis pathway (2021-05-31)
期刊:PeerJ
影响因子 :2.7
引用产品: Hep G2 细胞 , AGS 细胞 , HCT 116 细胞 , DLD-1 细胞 , HT-29 细胞
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Ubiquitination-mediated degradation of SIRT1 by SMURF2 suppresses CRC cell proliferation and tumorigenesis (2020-05-02)
期刊:Oncogene
影响因子 :8.0
引用产品: 293T [HEK-293T] 细胞 , HCT 116 细胞 , DLD-1 细胞
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miR-145-5p restrained cell growth, invasion, migration and tumorigenesis via modulating RHBDD1 in colorectal cancer via the EGFR-associated signaling pathway (2019-11-03)
期刊:International Journal Of Biochemistry & Cell Biology
DOI:10.1016/j.biocel.2019.105641
影响因子 :4.0
引用产品: Caco-2 细胞 , DLD-1 细胞 , LoVo 细胞 , SW480 [SW-480] 细胞 , T84 细胞
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