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产品概述
| 名称 | COLO 205 (人结肠癌细胞) (STR鉴定正确) |
| 别称 | Colo 205; CoLo 205; COLO-205; Colo-205; COLO.205; Colo205; COLO205; Co 205 |
| 种属 | 人 |
| 生长特性 | 半贴半悬 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3天/次 |
| 背景描述 | COLO 205细胞是由T·U·Sample等人于1957年从患有结肠癌的70岁男性白人的腹水分离而建系的，该病人在取腹水样品前已用5-氟尿嘧啶治疗4-6周；COLO 205细胞角蛋白免疫过氧化物酶染色阳性。 |
| 年龄（性别） | 男性；70岁 |
| 组织来源 | 结直肠腺癌，腹水转移 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肠癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~25-30 hours |
| 致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
| 基因表达情况 | carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10^6 cells cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10), The cells are positive for keratin by immunoperoxidase staining. |
| 保藏机构 | ATCC; CCL-222 BCRC; 60054 ECACC; 87061208 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11,12 D2S1338 17,18 D3S1358 16 D5S818 10,13 D7S820 9,10 D8S1179 9,14 D13S317 10,12 D16S539 12,13 D18S51 18 D19S433 13,14 D21S11 30.2,33.2 FGA 21,23 PentaD 9,11 PentaE 13,15 TH01 8,9 TPOX 11 vWA 15 D6S1043 11 D12S391 15 D2S441 10,11 -
STR鉴定图
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参考文献
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Raltitrexed as a synergistic hyperthermia chemotherapy drug screened in patient-derived colorectal cancer organoids (2008-01-20)
作者：Lisi Zeng:1.Institute of Oncology, Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou 510095, China. ; Quanxing Liao:1.Department of Abdominal Surgery, Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou 510095, China. ; Quanxing Zhao:1.Institute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China. ; 2.Tsinghua-Berkeley Shenzhen Institute (TBSI), Tsinghua University, Shenzhen 518055, China. ; Shengwei Jiang:1.Institute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China. ; 2.Tsinghua-Berkeley Shenzhen Institute (TBSI), Tsinghua University, Shenzhen 518055, China. ; 3.Shenzhen Key Laboratory of Gene and Antibody Therapy, Tsinghua University, Shenzhen 518055, China. ; Xianzi Yang:1.Department of Medical Oncology, Affiliated Cancer Hospital and Institute of Guangzhou Me
期刊：Raltitrexed as a synergistic hyperthermia chemotherapy drug screened in patient-derived colorectal cancer organoids
DOI：10.20892/j.issn.2095-3941.2020.0566
影响因子 ：5.5
引用产品： SW620 细胞 , SW480 [SW-480] 细胞 , DLD-1 细胞 , COLO 205 细胞
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Long non-coding RNA HOXA-AS3 facilitates the malignancy in colorectal cancer by miR-4319/SPNS2 axis (2010-01-20)
作者：Yang Jiang:1.Department of Pathology, Harbin Medical University Cancer Hospital, No. 150, Haping Road, Harbin, 150081, Heilongjiang, China. ; Xiao-Yu Yu:1.Department of Pathology, Harbin Medical University Cancer Hospital, No. 150, Haping Road, Harbin, 150081, Heilongjiang, China. ; Hui-Xin Sun:1.Institute of Cancer Prevention and Treatment, Harbin Medical University, Harbin, 150081, Heilongjiang, China. ; Xin-Yue Gu:1.Department of Colorectal Surgery, Harbin Medical University Cancer Hospital, No. 150, Haping Road, Harbin, 150081, Heilongjiang, China. dr_gxy@163.com. ; Jing-Shu Geng:1.Department of Pathology, Harbin Medical University Cancer Hospital, No. 150, Haping Road, Harbin, 150081, Heilongjiang, China. gengjingshu@foxmail.com. ;
期刊：Long non-coding RNA HOXA-AS3 facilitates the malignancy in colorectal cancer by miR-4319/SPNS2 axis
DOI：10.1007/s13105-021-00832-x
影响因子 ：5.1
引用产品： SW480 [SW-480] 细胞 , SW620 细胞 , HCT 116 细胞 , COLO 205 细胞 , LoVo 细胞
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SPNS2 promotes the malignancy of colorectal cancer cells via regulating Akt and ERK pathway (2006-09-20)
作者：Xinyue Gu:1.Department of Colorectal Surgery, Harbin Medical University Cancer Hospital, Harbin, China. ; Yang Jiang:1.Department of Pathology, Harbin Medical University Cancer Hospital, Harbin, China. ; Weinan Xue:1.Department of Colorectal Surgery, Harbin Medical University Cancer Hospital, Harbin, China. ; Chengxin Song:1.Department of Colorectal Surgery, Harbin Medical University Cancer Hospital, Harbin, China. ; Yangyang Wang:1.Department of Colorectal Surgery, Harbin Medical University Cancer Hospital, Harbin, China. ; Yanlong Liu:1.Department of Colorectal Surgery, Harbin Medical University Cancer Hospital, Harbin, China. ; Binbin Cui:1.Department of Colorectal Surgery, Harbin Medical University Cancer Hospital, Harbin, China. ;
期刊：SPNS2 promotes the malignancy of colorectal cancer cells via regulating Akt and ERK pathway
影响因子 ：2.9
引用产品： SW480 [SW-480] 细胞 , SW620 细胞 , HCT 116 细胞 , COLO 205 细胞 , LoVo 细胞
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miRNA-101通过靶向CDK8调控结肠癌细胞侵袭和Wnt/β-catenin通路活化 (2005-08-20)
作者：Department of General Surgery, Baoshan People's Hospital, Baoshan 678000, China Depatrment of General Surgery, The First People's Hospital of Yunnan Province, Kunming 650021, China Department of Nursing, The Chinese People's Liberation Army 105 Hospital, Hefei 230001, China
期刊：miRNA-101通过靶向CDK8调控结肠癌细胞侵袭和Wnt/β-catenin通路活化
DOI：10.3971/j.issn.1000-8578.2018.17.1558
影响因子 ：0.7
引用产品： HCT 116 细胞 , HT-29 细胞 , LoVo 细胞 , SW620 细胞 , COLO 205 细胞
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