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产品概述
| 名称 | Hela S3 (人宫颈癌细胞) (STR鉴定正确) |
| 别称 | HeLa s3; HeLa-S3; HELA-S3; HeLa/S3; HeLa.S3; HeLa S 3; HeLa S-3; HeLaS3; S3-HeLa; S3 HeLa |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
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| 推荐传代比例 | 1:3-1:8 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | Hela S3细胞是由Puck·T·T、Marcus·P·I和Cieciura·S·J于1955年建系的。Hela S3细胞含HPV-18序列；角蛋白阳性。Hela S3细胞可用于与染色体突变、细胞营养、集落形成相关的哺乳动物细胞的克隆分析。 |
| 年龄（性别） | 女性；31岁 |
| 组织来源 | 子宫颈 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 宫颈癌细胞 |
| 生物安全等级 | BSL-2 [Cells contain human papilloma virus (HPV-18)] |
| 倍增时间 | ~48 hours |
| 保藏机构 | ATCC; CCL-2.2 ATCC; CRL-7924DSMZ; ACC-161 ECACC; 87110901 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 9,10 D2S1338 17 D3S1358 15,18 D5S818 11,12 D7S820 8,12 D8S1179 12,13 D13S317 13.3 D16S539 9,10 D18S51 16 D19S433 13,14 D21S11 27,28 FGA 18,21 PentaD 8,15 PentaE 7,17 TH01 7 TPOX 8,12 vWA 16,18 D6S1043 18,19 D12S391 20,25 D2S441 10,11 -
STR鉴定图
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参考文献
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Farnesyl diphosphate synthase promotes cell proliferation by regulating gene expression and alternative splicing profiles in HeLa cells (2023-02-28)
作者：Lijuan Wang:1.Department of Endocrinology, The Second Hospital of Jilin University, Changchun, Jilin 130021, P.R. China. ; Zhigang Chen:1.ABLife BioBigData Institute, Wuhan, Hubei 430075, P.R. China. ; Dong Chen:1.ABLife BioBigData Institute, Wuhan, Hubei 430075, P.R. China. ; Bo Kan:1.Department of Clinical Laboratory, The Second Hospital of Jilin University, Changchun, Jilin 130021, P.R. China. ; Yangfang He:1.Department of Endocrinology, The Second Hospital of Jilin University, Changchun, Jilin 130021, P.R. China. ; Hanqing Cai:1.Department of Endocrinology, The Second Hospital of Jilin University, Changchun, Jilin 130021, P.R. China. ;
期刊：Farnesyl diphosphate synthase promotes cell proliferation by regulating gene expression and alternative splicing profiles in HeLa cells
影响因子 ：2.9
引用产品： Hela S3 细胞 , HOS 细胞 , MEM (含NEAA) 培养基
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Transcription factor Dp-1 knockdown downregulates thymidine kinase 1 expression to protect against proliferation and epithelial-mesenchymal transition in cervical cancer (2023-09-16)
作者：Mei Wu:1.Department of Gynecologic Oncology, Hunan Cancer Hospital, Changsha, Hunan, 410013, People's Republic of China. ; Mingji Ye:1.Department of Urology Surgery, Hunan Cancer Hospital, No. 283, Tongzipo Road, Yuelu District, Changsha, Hunan, 410013, People's Republic of China. yemingji198307@163.com. ;
期刊：Transcription factor Dp-1 knockdown downregulates thymidine kinase 1 expression to protect against proliferation and epithelial-mesenchymal transition in cervical cancer
DOI：10.1007/s10142-023-01218-6
影响因子 ：2.9
引用产品： C-33 A 细胞 , Ca Ski 细胞 , Hela S3 细胞 , SiHa 细胞 , 人子宫颈上皮细胞
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HPV16+-miRNAs in cervical cancer and the anti-tumor role played by miR-5701 (2010-09-20)
作者：Nuerbieke Pulati:1.Department of Radiation Oncology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China. ; Zegao Zhang:1.Department of Radiation Oncology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China. ; Aireti Gulimilamu:1.Department of Radiation Oncology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China. ; Xiaoli Qi:1.Department of Radiation Oncology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China. ; Jie Yang:1.Department of Radiation Oncology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China. ;
期刊：HPV16+-miRNAs in cervical cancer and the anti-tumor role played by miR-5701
DOI：10.1002/jgm.3126
影响因子 ：4.2
引用产品： C-33 A 细胞 , Hela S3 细胞
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