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| 名称 | Ca Ski (人宫颈癌肠转移细胞/人宫颈癌上皮细胞) (STR鉴定正确) |
| 别称 | Ca-Ski; CaSki; Caski; CASKI |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
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| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | Ca Ski细胞是从小肠肠系膜转移灶的细胞中建立的。据报道，Ca Ski细胞含有完整的HPV-16(每个细胞大约600个拷贝)和HPV-18相关序列。 |
| 年龄（性别） | 女性；40岁 |
| 组织来源 | 宫颈癌肠转移 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 宫颈癌细胞 |
| 生物安全等级 | BSL-2 |
| 倍增时间 | ~60-80 hours |
| 基因表达情况 | beta subunit of human chorionic gonadotropin (hCG); tumor associated antigen |
| 保藏机构 | ATCC; CRL-1550 ATCC; CRM-CRL-1550 ATCC; CRL-7915BCRC; 60251 ECACC; 87020501 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10 D2S1338 21 D3S1358 15 D5S818 13 D7S820 8,11 D8S1179 15 D13S317 8,12 D16S539 11,12 D18S51 17 D19S433 15,16 D21S11 30 FGA 21 PentaD 10 PentaE 5 TH01 7 TPOX 8 vWA 17 D6S1043 11,14 D12S391 18 D2S441 11 -
STR鉴定图
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参考文献
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Function and mechanism of GBP1 in the development and progression of cervical cancer (2024-01-02)
期刊：Journal of Translational Medicine
DOI：10.1186/s12967-023-04837-6
影响因子 ：7.4
引用产品： Ca Ski 细胞 , DMEM高糖 培养基
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HIF-1A as a prognostic biomarker related to invasion, migration and immunosuppression of cervical cancer (2024-01-13)
期刊：Heliyon
DOI：10.1016/j.heliyon.2024.e24664
影响因子 ：4.0
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APLN promotes the proliferation, migration, and glycolysis of cervical cancer through the PI3K/AKT/mTOR pathway (2024-03-30)
期刊：ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
影响因子 ：3.9
引用产品： C-33 A 细胞 , Hela 细胞 , SiHa 细胞 , Ca Ski 细胞 , ECT1/E6E7 细胞 , 人子宫颈上皮细胞完全培养基
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PD-L1 Exon 3 Is a Hidden Switch of Its Expression and Function in Oral Cancer Cells (2023-05-03)
期刊：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 ：5.6
引用产品： FaDu 细胞 , U-2 OS [U2OS] 细胞 , Ca Ski 细胞
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An anti-PD-1 antisense oligonucleotide promotes the expression of soluble PD-1 by blocking the interaction between SRSF3 and an exonic splicing enhancer of PD-1 exon 3 (2023-12-04)
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2023.111280
影响因子 ：5.6
引用产品： MOLT-4 细胞 , FaDu 细胞 , Ca Ski 细胞 , HCT 116 细胞
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IGF2BP3-mediated regulation of GLS and GLUD1 gene expression promotes treg-induced immune escape in human cervical cancer (2023-11-15)
期刊：American Journal of Cancer Research
DOI：PMID:38058838
影响因子 ：5.3
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HPV E6 promotes cell proliferation of cervical cancer cell by accelerating accumulation of RBM15 dependently of autophagy inhibition (2023-05-16)
期刊：CELL BIOLOGY INTERNATIONAL
影响因子 ：3.9
引用产品： C-33 A 细胞 , Ca Ski 细胞 , SiHa 细胞 , Hela 细胞 , MEM (含NEAA) 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100× , RPMI-1640 培养基 , DMEM高糖 培养基 , McCoy's 5A 培养基 , DMEM/F12 培养基
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Characterization of the microenvironment in different immune-metabolism subtypes of cervical cancer with prognostic significance (2023-02-03)
期刊：Frontiers in Genetics
DOI：10.3389/fgene.2023.1067666
影响因子 ：3.7
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Circ_0005615 promotes cervical cancer cell growth and metastasis by modulating the miR-138-5p/KDM2A axis (2023-07-02)
期刊：JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
影响因子 ：3.6
引用产品： SiHa 细胞 , Ca Ski 细胞 , 293T [HEK-293T] 细胞 , MEM 培养基 , RPMI-1640 培养基
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LINC00460 knockdown sensitizes cervical cancer to cisplatin by downregulating TGFBI (2023-12-28)
期刊：Chemical Biology & Drug Design
影响因子 ：3.0
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Asperuloside Promotes Apoptosis of Cervical Cancer Cells through Endoplasmic Reticulum Stress-Mitochondrial Pathway (2023-04-20)
期刊：Chinese Journal of Integrative Medicine
影响因子 ：2.9
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SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A (2023-04-21)
期刊：Oncology Letters
影响因子 ：2.9
引用产品： SiHa 细胞 , C-33 A 细胞 , Ca Ski 细胞 , DMEM/F12 培养基
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Transcription factor Dp-1 knockdown downregulates thymidine kinase 1 expression to protect against proliferation and epithelial-mesenchymal transition in cervical cancer (2023-09-16)
期刊：FUNCTIONAL & INTEGRATIVE GENOMICS
DOI：10.1007/s10142-023-01218-6
影响因子 ：2.9
引用产品： C-33 A 细胞 , Ca Ski 细胞 , Hela S3 细胞 , SiHa 细胞 , 人子宫颈上皮细胞
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BTG2 suppresses the growth and metastasis of cervical squamous cell carcinoma (2023-05-26)
期刊：PATHOLOGY RESEARCH AND PRACTICE
影响因子 ：2.8
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TRIM21 promotes tumor progression and cancer stemness in cervical squamous cell carcinoma (2023-07-23)
期刊：PATHOLOGY RESEARCH AND PRACTICE
影响因子 ：2.8
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Circular RNA circ_0000119 promotes cervical cancer cell growth and migration via miR-433-3p/PAK2 axis (2023-08-04)
期刊：JOURNAL OF APPLIED GENETICS
DOI：10.1007/s13353-023-00772-w
影响因子 ：2.4
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Cellular Retinoic Acid Binding Protein 2 (CRABP2), Up-regulated by HPV E6/E7, Leads to Aberrant Activation of the Integrin β1/FAK/ERK Signaling Pathway and Aggravates the Malignant Phenotypes of Cervical Cancer (2023-11-24)
期刊：BIOCHEMICAL GENETICS
DOI：10.1007/s10528-023-10568-6
影响因子 ：2.4
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PLAC8 contributes to the malignant behaviors of cervical cancer cells by activating the SOX4-mediated AKT pathway (2023-01-05)
期刊：HISTOCHEMISTRY AND CELL BIOLOGY
DOI：10.1007/s00418-022-02175-0
影响因子 ：2.3
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CDK6 increases glycolysis and suppresses autophagy by mTORC1-HK2 pathway activation in cervical cancer cells (2002-02-20)
期刊：Cell Cycle
DOI：10.1080/15384101.2022.2039981
影响因子 ：5.2
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Signature of seven cuproptosis-related lncRNAs as a novel biomarker to predict prognosis and therapeutic response in cervical cancer (2022-09-20)
期刊：Frontiers in Genetics
影响因子 ：4.8
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OTX1 promotes tumorigenesis and progression of cervical cancer by regulating the Wnt signaling pathway (2022-09-29)
期刊：ONCOLOGY REPORTS
影响因子 ：4.1
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ZIC5 promotes aggressiveness and cancer stemness in cervical squamous cell carcinoma (2022-12-05)
期刊：PATHOLOGY RESEARCH AND PRACTICE
影响因子 ：3.3
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FBXO39 predicts poor prognosis and correlates with tumor progression in cervical squamous cell carcinoma (2022-08-28)
期刊：PATHOLOGY RESEARCH AND PRACTICE
影响因子 ：3.3
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hsa_circ_0119412 overexpression promotes cervical cancer progression by targeting miR-217 to upregulate anterior gradient 2 (2003-02-20)
期刊：Journal Of Clinical Laboratory Analysis
影响因子 ：3.1
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tRNA‑derived fragment tRF‑Glu49 inhibits cell proliferation, migration and invasion in cervical cancer by targeting FGL1 (2022-08-09)
期刊：Oncology Letters
影响因子 ：3.1
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Circ_0042986 Presence Restrains Cervical Cancer Development via Upregulating PEG3 by Directly Targeting miR-582-3p (2022-08-26)
期刊：Reproductive Sciences
DOI：10.1007/s43032-022-01053-3
影响因子 ：2.9
引用产品： Hela 细胞 , Ca Ski 细胞 , HUVECs cell
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Circular RNA circVPRBP serves as a microRNA-106b-5p sponge to regulate proliferation and metastasis of cervical cancer cells via tripartite motif-containing protein 3 (2022-08-10)
期刊：ANTI-CANCER DRUGS
DOI：10.1097/CAD.0000000000001335
影响因子 ：2.4
引用产品： Ca Ski 细胞 , SiHa 细胞 , SiHa细胞专用培养基
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LncRNA Opa interacting protein 5-antisense RNA 1 (OIP5-AS1) promotes the migration, invasion and epithelial-mesenchymal transition (EMT) through targeting miR-147a/insulin-like growth factor 1 receptor (IGF1R) pathway in cervical cancer tissues and c (2003-02-20)
期刊：Journal Of Obstetrics And Gynaecology Research
影响因子 ：1.6
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Human bone marrow mesenchymal stem cell-derived extracellular vesicles impede the progression of cervical cancer via the miR-144-3p/CEP55 pathway (2006-01-20)
期刊：Journal Of Cellular And Molecular Medicine
影响因子 ：5.3
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T-box transcription factor TBX1, targeted by microRNA-6727-5p, inhibits cell growth and enhances cisplatin chemosensitivity of cervical cancer cells through AKT and MAPK pathways (2002-01-20)
期刊：Bioengineered
DOI：10.1080/21655979.2021.1880732
影响因子 ：4.9
引用产品： Ca Ski 细胞 , RPMI-1640 培养基 , SiHa 细胞 , MEM (含NEAA) 培养基
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Vitamin E succinate exerts anti-tumour effects on human cervical cancer cells via the CD47-SIRPɑ pathway both in vivo and in vitro (2005-01-20)
期刊：Journal of Cancer
影响因子 ：3.9
引用产品： Ca Ski 细胞
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ASF1B promotes cervical cancer progression through stabilization of CDK9 (2008-00-20)
期刊：Cell Death & Disease
DOI：10.1038/s41419-020-02872-5
影响因子 ：9.0
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KMT2A regulates cervical cancer cell growth through targeting VDAC1. (2005-00-20)
期刊：Aging-US
影响因子 ：5.2
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Long non‑coding RNA AK001903 regulates tumor progression in cervical cancer (2011-00-20)
期刊：Oncology Letters
影响因子 ：2.9
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MiRNA-211 triggers an autophagy-dependent apoptosis in cervical cancer cells: regulation of Bcl-2 (2010-09-20)
期刊：Naunyn-Schmiedebergs Archives of Pharmacology
DOI：10.1007/s00210-019-01720-4
影响因子 ：3.6
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Inhibition of miR-574-5p suppresses cell growth and metastasis and enhances chemosensitivity by targeting RNA binding protein QKI in cervical cancer cells (2011-09-20)
期刊：Naunyn-Schmiedebergs Archives of Pharmacology
DOI：10.1007/s00210-019-01772-6
影响因子 ：3.6
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