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产品概述
名称 | BT-20 (人乳腺癌细胞) (STR鉴定正确) |
别称 | BT 20; BT20 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | BT-20细胞是由E·Y·Lasfargues和L·Ozzello于1958年建系,源自一位74岁白人女性的乳腺癌组织。BT-20细胞表达WNT3和WNT78;TNF-α抑制BT-20细胞生长。BT-20细胞雌激素受体阴性,但表达5'-外显子缺失的雌激素mRNA。 |
年龄(性别) | 女性;74岁 |
组织来源 | 乳腺;侵袭性导管癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 乳腺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~70 hours |
致瘤性 | Yes, in nude mice (The cells form grade II adenocarcinomas). |
抗原表达情况 | HLA A1, Bw16 (+/-) |
保藏机构 | ATCC; CRL-7912ATCC; HTB-19 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 19 D3S1358 17 D5S818 12 D7S820 10 D8S1179 12 D13S317 11 D16S539 11,14 D18S51 17 D19S433 15 D21S11 28,29 FGA 22,24 PentaD 10,11 PentaE 11,13 TH01 7,9.3 TPOX 11 vWA 16,17 D6S1043 19 D12S391 18.3,24 D2S441 10,14 -
STR鉴定图
参考文献
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The dePARylase NUDT16 promotes radiation resistance of cancer cells by blocking SETD3 for degradation via reversing its ADP-ribosylation (2024-01-22)
作者:Weijun Wu:1.Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China; Department of Oncology Radiotherapy, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, China. ; Wenjing Wu:1.Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China; Department of Breast Oncology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China. ; Yingshi Zhou:1.Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, G
期刊:The dePARylase NUDT16 promotes radiation resistance of cancer cells by blocking SETD3 for degradation via reversing its ADP-ribosylation
影响因子 :4.8
引用产品: MCF 10A 细胞 , HCC1806 细胞 , HCC1937 细胞 , MDA-MB-468 细胞 , BT-20 细胞 , Hs 578T 细胞 , MDA-MB-436 细胞 , MCF 10A细胞专用培养基 , HCC1806细胞专用培养基 , HCC1937细胞专用培养基 , MDA-MB-468细胞专用培养基 (L15) , BT-20细胞专用培养基 , Hs 578T细胞专用培养基 , MDA-MB-436细胞专用培养基 (L15)
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LINC01234 promoted malignant behaviors of breast cancer cells via hsa-miR-30c-2-3p/CCT4/mTOR signaling pathway (2024-01-11)
作者:Chuangang Tang:1.Suzhou Medical College of Soochow University, Suzhou, Jiangsu, 215123, China; Department of Breast Surgery, Xuzhou Central Hospital, Postgraduate Workstation of Soochow University Xuzhou, Jiangsu, 221009, China. ; Changwen Li:1.Department of Breast Surgery, Xuzhou Central Hospital, Jiangsu, 221009, China. ; Chengling Chen:1.Department of Breast Surgery, Xuzhou Central Hospital, Jiangsu, 221009, China. ; Tao Chen:1.The Xuzhou Clinical College of Xuzhou Medical University, Jiangsu, 221009, China. ; Juan Zhu:1.Department of Breast Surgery, Xuzhou Central Hospital, Jiangsu, 221009, China. ; Mingyu Sun:1.Department of Breast Surgery, Xuzhou Central Hospital, Jiangsu, 221009, China. Electronic address: 645396517@qq.com. ; Pei Wang:1.Department of Breast Surgery, Xuzhou Central Hospital, Jiangsu, 221009, China. Electronic address: 1349533289@qq.com. ; Conghui Han:1.Suzhou Medical College of Soochow University, Suzhou, Jiangsu, 215123, China; Department of Urology, Xuzh
期刊:LINC01234 promoted malignant behaviors of breast cancer cells via hsa-miR-30c-2-3p/CCT4/mTOR signaling pathway
DOI:10.1016/j.tjog.2023.09.019
影响因子 :2.1
引用产品: BT-20 细胞
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Leveraging senescence-oxidative stress co-relation to predict prognosis and drug sensitivity in breast invasive carcinoma (2023-08-04)
作者:Yinghui Ye:1.Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China. ; Yulou Luo:1.Department of Breast Surgery, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, China. ; Tong Guo:1.Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China. ; Chenguang Zhang:1.Department of Breast Surgery, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, China. ; Yutian Sun:1.Department of Medical Oncology, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China. ; Anping Xu:1.Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China. ; Ling Ji:1.Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China. ; Jianghua Ou:1.Department of Breast Surgery, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, China. ; Shang Ying Wu:1.Depa
期刊:Leveraging senescence-oxidative stress co-relation to predict prognosis and drug sensitivity in breast invasive carcinoma
DOI:10.3389/fendo.2023.1179050
影响因子 :5.2
引用产品: MCF7 [MCF-7] 细胞 , BT-20 细胞 , MDA-MB-231 细胞 , HCC1806 细胞 , HCC1937 细胞 , MCF7 [MCF-7]细胞专用培养基 , BT-20细胞专用培养基 , MDA-MB-231细胞专用培养基 (L15) , HCC1806细胞专用培养基 , HCC1937细胞专用培养基
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Gankyrin inhibits ferroptosis through the p53/SLC7A11/GPX4 axis in triple-negative breast cancer cells (2023-12-08)
作者:Ming Lei:1.Department of Breast and Thyroid Surgery, The Second Affiliated Hospital, Hainan Medical University, Haikou, 570311, China. ; 2.Key Laboratory of Tropical Translational Medicine of Ministry of Education & School of Tropical Medicine, Hainan Medical University, Haikou, 571199, China. ; Yun-Long Zhang:1.Key Laboratory of Tropical Translational Medicine of Ministry of Education & School of Tropical Medicine, Hainan Medical University, Haikou, 571199, China. ; Feng-Ying Huang:1.Key Laboratory of Tropical Translational Medicine of Ministry of Education & School of Tropical Medicine, Hainan Medical University, Haikou, 571199, China. ; Heng-Yu Chen:1.Department of Breast and Thyroid Surgery, The Second Affiliated Hospital, Hainan Medical University, Haikou, 570311, China. ; Ming-Hui Chen:1.Key Laboratory of Tropical Translational Medicine of Ministry of Education & School of Tropical Medicine, Hainan Medical University, Haikou, 571199, China. ; Ri-Hong Wu:1.Key Laboratory of T
期刊:Gankyrin inhibits ferroptosis through the p53/SLC7A11/GPX4 axis in triple-negative breast cancer cells
DOI:10.1038/s41598-023-49136-8
影响因子 :4.6
引用产品: MDA-MB-231 细胞 , HCC1937 细胞 , MDA-MB-468 细胞 , BT-20 细胞 , Hs 578T 细胞 , MCF 10A 细胞
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Prognosis stratification of patients with breast invasive carcinoma based on cysteine metabolism-disulfidptosis affinity (2023-07-08)
作者:Yuting Zhang:1.Department of Breast Surgery, The First Affiliated Hospital, Jinan University, Guangzhou, 510000, China. ; Yinghui Ye:1.Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, 518000, China. ; Anping Xu:1.Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, 518000, China. ; Yulou Luo:1.Department of Breast Surgery, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, 830000, China. ; Yutian Sun:1.Department of Medical Oncology, School of Medicine, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, 610000, China. ; Wei Zhang:1.Department of Breast Surgery, The First Affiliated Hospital, Jinan University, Guangzhou, 510000, China. zhangwei@jnu.edu.cn. ; Ling Ji:1.Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, 518000, China. 1120303921@qq.com. ;
期刊:Prognosis stratification of patients with breast invasive carcinoma based on cysteine metabolism-disulfidptosis affinity
DOI:10.1007/s00432-023-05028-y
影响因子 :3.6
引用产品: MCF7 [MCF-7] 细胞 , MCF7 [MCF-7]细胞专用培养基 , BT-20 细胞 , BT-20细胞专用培养基 , MDA-MB-231 细胞 , MDA-MB-231细胞专用培养基 (L15) , MDA-kb2 细胞 , MDA-kb2细胞专用培养基 (L15) , HCC1937 细胞 , HCC1937细胞专用培养基
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EXOSC2 Mediates the Pro-tumor Role of WTAP in Breast Cancer Cells via Activating the Wnt/β-Catenin Signal (2023-10-19)
作者:Chen-Guang Lv:1.Department of General Surgery, General Hospital of Northern Theater Command, Shenyang, People's Republic of China. ; Yao Cheng:1.Department of Oncology, Panjin Central Hospital, Panjin, People's Republic of China. ; Lei Zhang:1.Department of Breast Surgery, The First Hospital of China Medical University, No. 155, Nanjing North Street, Shenyang, People's Republic of China. ; Guo-Gang Wu:1.Department of Thyroid and Breast Surgery, Ansteel Group General Hospital, Anshan, People's Republic of China. ; Chun-Yan Liang:1.Department of Oncology, The Fourth Affiliated Hospital of China Medical University, Shenyang, People's Republic of China. ; Zuo Tao:1.Department of General Surgery, General Hospital of Northern Theater Command, Shenyang, People's Republic of China. ; Bo Chen:1.Department of Breast Surgery, The First Hospital of China Medical University, No. 155, Nanjing North Street, Shenyang, People's Republic of China. chenbocmu@sina.com. ;
期刊:EXOSC2 Mediates the Pro-tumor Role of WTAP in Breast Cancer Cells via Activating the Wnt/β-Catenin Signal
DOI:10.1007/s12033-023-00834-8
影响因子 :2.6
引用产品: BT-20 细胞
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The inhibition of centromere protein K causes anticancer effects in breast carcinoma via effects on the FAK/PI3K/AKT/mTOR pathway (2022-09-09)
作者:Jiao Yu:1.Department of Radiation Oncology, Shaanxi Provincial People's Hospital, Xi'an 710068, China. ; Kai Wang:1.Department of Traditional Chinese Medicine, Xi'an Chang'an District Hospital, Xi'an 710119, China. ; Sanhu Yang:1.Department of Thoracic Surgery, Tangdu Hospital, Air Force Military Medical University, Xi'an 710049, China. ; Gang Li:1.Department of Thyroid and Breast Surgery, Xi'an Daxing Hospital, Xi'an 710068, China. Electronic address: dooopenitlg@163.com. ;
期刊:The inhibition of centromere protein K causes anticancer effects in breast carcinoma via effects on the FAK/PI3K/AKT/mTOR pathway
DOI:10.1016/j.taap.2022.116232
影响因子 :4.5
引用产品: T-47D 细胞 , Hs 578T 细胞 , MCF7 [MCF-7] 细胞 , BT-20 细胞 , BT-549 细胞 , MCF 10A 细胞 , RPMI-1640 培养基 , MEM 培养基 , DMEM/F12 培养基
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