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产品概述
名称 | BT-20 (人乳腺癌细胞) (STR鉴定正确) |
别称 | BT 20; BT20 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | BT-20细胞是由E·Y·Lasfargues和L·Ozzello于1958年建系,源自一位74岁白人女性的乳腺癌组织。BT-20细胞表达WNT3和WNT78;TNF-α抑制BT-20细胞生长。BT-20细胞雌激素受体阴性,但表达5'-外显子缺失的雌激素mRNA。 |
年龄(性别) | 女性;74岁 |
组织来源 | 乳腺;侵袭性导管癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 乳腺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~70 hours |
致瘤性 | Yes, in nude mice (The cells form grade II adenocarcinomas). |
抗原表达情况 | HLA A1, Bw16 (+/-) |
保藏机构 | ATCC; CRL-7912ATCC; HTB-19 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 19 D3S1358 17 D5S818 12 D7S820 10 D8S1179 12 D13S317 11 D16S539 11,14 D18S51 17 D19S433 15 D21S11 28,29 FGA 22,24 PentaD 10,11 PentaE 11,13 TH01 7,9.3 TPOX 11 vWA 16,17 D6S1043 19 D12S391 18.3,24 D2S441 10,14 -
STR鉴定图
参考文献
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SOD1-high fibroblasts derived exosomal miR-3960 promotes cisplatin resistance in triple-negative breast cancer by suppressing BRSK2-mediated phosphorylation of PIMREG(2024/04/04)
作者:Kangdi Li, Han Lin, Anyi Liu
期刊:CANCER LETTERS
DOI:10.1016/j.canlet.2024.216842
影响因子 :9.700
引用产品:CL-0324
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Leveraging senescence-oxidative stress co-relation to predict prognosis and drug sensitivity in breast invasive carcinoma(2023/08/04)
作者:Yinghui Ye, Yulou Luo, Tong Guo, Chenguang Zhang, Yutian Sun, Anping Xu, Ling Ji, Jianghua Ou, Shang Ying Wu
期刊:Frontiers in Endocrinology
DOI:10.3389/fendo.2023.1179050
影响因子 :5.200
引用产品:CL-0093 CL-0149 CL-0324 CL-0726 CM-0093 CM-0149 CM-0324 CM-0726
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The dePARylase NUDT16 promotes radiation resistance of cancer cells by blocking SETD3 for degradation via reversing its ADP-ribosylation(2024/01/22)
作者:Weijun Wu, Wenjing Wu, Yingshi Zhou
期刊:JOURNAL OF BIOLOGICAL CHEMISTRY
影响因子 :4.800
引用产品:CL-0324
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H3K27ac-induced RHOXF2 activates Wnt2/β-catenin pathway by binding to HOXC13 to aggravate the malignant progression of triple negative breast cancer(2024/04/30)
作者:Man Guo, Ruoyan Wang, Mandi Nie
期刊:CELLULAR SIGNALLING
DOI:10.1016/j.cellsig.2024.111196
影响因子 :4.800
引用产品:CL-0324
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Gankyrin inhibits ferroptosis through the p53/SLC7A11/GPX4 axis in triple-negative breast cancer cells(2023/12/08)
作者:Ming Lei, Yunlong Zhang, Fengying Huang
期刊:Scientific Reports
DOI:10.1038/s41598-023-49136-8
影响因子 :4.600
引用产品:CL-0324
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The inhibition of centromere protein K causes anticancer effects in breast carcinoma via effects on the FAK/PI3K/AKT/mTOR pathway(2022/09/09)
作者:Jiao Yu, Kai Wang, Sanhu Yang
期刊:TOXICOLOGY AND APPLIED PHARMACOLOGY
DOI:10.1016/j.taap.2022.116232
影响因子 :3.800
引用产品:CL-0041 CL-0114 CL-0149 CL-0228 CL-0324 CL-0525 PM150110 PM150312 PM150411
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Prognosis stratification of patients with breast invasive carcinoma based on cysteine metabolism-disulfidptosis affinity(2023/07/08)
作者:Yuting Zhang, Yinghui Ye, Anping Xu
期刊:JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
DOI:10.1007/s00432-023-05028-y
影响因子 :3.600
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EXOSC2 Mediates the Pro-tumor Role of WTAP in Breast Cancer Cells via Activating the Wnt/β-Catenin Signal(2023/10/19)
作者:Chenguang Lv, Yao Cheng, Lei Zhang
期刊:MOLECULAR BIOTECHNOLOGY
DOI:10.1007/s12033-023-00834-8
影响因子 :2.600
引用产品:CL-0324
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LINC01234 promoted malignant behaviors of breast cancer cells via hsa-miR-30c-2-3p/CCT4/mTOR signaling pathway(2024/01/11)
作者:Chuangang Tang, Changwen Li, Chengling Chen
期刊:Taiwanese Journal of Obstetrics & Gynecology
DOI:10.1016/j.tjog.2023.09.019
影响因子 :2.100
引用产品:CL-0324
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