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产品概述
名称 | MDA-MB-468 (人乳腺癌细胞) (STR鉴定正确) |
别称 | MDA-MB 468; MDA-MB468; MDAMB468; MDA-468; MDA468; MB468; MD Anderson-Metastatic Breast-468 |
种属 | 人类 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,100%, 温度:37℃
|
培养方案B(可选) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 | 1.该细胞推荐使用Leibovitz's L-15培养基进行培养,Leibovitz's L-15不可以通入二氧化碳,会产生细胞毒性;2.如您没有无二氧化碳的培养箱,可使用DMEM替代Leibovitz's L-15,使用DMEM培养基时即可正常通入5%二氧化碳;3.配套专用培养基默认Leibovitz's L-15配置,如需DMEM配方,请联系销售下单备注更改。4.该细胞培养过程中会分泌少量黑色颗粒物,不影响细胞生长,属正常现象。 |
背景描述 | MDA-MB-468细胞是由Cailleau·R等人于1977年从一位患有转移性乳腺癌的51岁黑人女性的胸腔积液中分离得到的。虽然供体组织的G6PD等位基因杂合,但MDA-MB-468细胞始终表现为G6PD A表型。p53MDA-MB-468细胞是由Cailleau·R等人于1977年从一位患有转移性乳腺癌的51岁黑人女性的胸腔积液中分离得到的。虽然供体组织的G6PD等位基因杂合,但MDA-MB-468细胞始终表现为G6PD A表型。p53基因273位密码子存在G→A突变,从而导致Arg→His替代。每个MDA-MB-468细胞上存在1×10^6个EGF受体。 |
年龄(性别) | 女性,51岁 |
组织来源 | 乳腺;乳房;腺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 乳腺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~36-62 hours |
致瘤性 | Yes, in nude mice inoculated subcutaneously with 1×10^7 cells.(Tumors developed within 21 days at 100% frequency (5/5).) |
受体表达情况 | epidermal growth factor (EGF); transforming growth factor alpha (TGF alpha) |
抗原表达情况 | Blood Type AB; HLA Aw23, Aw30, B27, Bw35, Cw2, Cw4 (patient) |
保藏机构 | ATCC; HTB-132 DSMZ; ACC-738 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 17,25 D3S1358 15 D5S818 12 D7S820 8 D8S1179 13 D13S317 12 D16S539 9 D18S51 17 D19S433 12,13 D21S11 27,28 FGA 23 PentaD 8,10 PentaE 5 TH01 7 TPOX 8,9 vWA 18 D6S1043 13,23 D12S391 18 D2S441 12,14 -
STR鉴定图
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参考文献
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Self-propelled assembly of nanoparticles with self-catalytic regulation for tumour-specific imaging and therapy (2024-01-11)
期刊:Nature Communications
DOI:10.1038/s41467-024-44736-y
影响因子 :16.6
引用产品: MDA-MB-468 细胞
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Single-shot deterministic complex amplitude imaging with a single-layer metalens (2024-01-05)
期刊:Science Advances
影响因子 :13.6
引用产品: MDA-MB-468 细胞
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Microfluidic Impedance Cytometry Enabled One-Step Sample Preparation for Efficient Single-Cell Mass Spectrometry (2024-03-14)
期刊:Small
影响因子 :13.3
引用产品: Hela 细胞 , MCF7 [MCF-7] 细胞 , MDA-MB-468 细胞
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UCHL1 contributes to insensitivity to endocrine therapy in triple-negative breast cancer by deubiquitinating and stabilizing KLF5 (2024-03-11)
期刊:BREAST CANCER RESEARCH
DOI:10.1186/s13058-024-01800-1
影响因子 :7.4
引用产品: MCF7 [MCF-7] 细胞 , BT-474 [BT474] 细胞 , SUM149PT 细胞 , MDA-MB-468 细胞
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TMEM120B strengthens breast cancer cell stemness and accelerates chemotherapy resistance via β1-integrin/FAK-TAZ-mTOR signaling axis by binding to MYH9 (2024-03-19)
期刊:BREAST CANCER RESEARCH
DOI:10.1186/s13058-024-01802-z
影响因子 :7.4
引用产品: ZR-75-1 细胞 , MDA-MB-468 细胞
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An estrogen-regulated long non-coding RNA NCALD promotes luminal breast cancer proliferation by activating GRHL2 (2024-01-30)
期刊:Cancer Cell International
DOI:10.1186/s12935-024-03245-0
影响因子 :5.8
引用产品: MDA-MB-231/GFP 细胞 , MDA-MB-468 细胞 , SK-BR-3 [SKBR3] 细胞 , MCF 10A 细胞 , ZR-75-1 细胞
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The dePARylase NUDT16 promotes radiation resistance of cancer cells by blocking SETD3 for degradation via reversing its ADP-ribosylation (2024-01-22)
期刊:JOURNAL OF BIOLOGICAL CHEMISTRY
影响因子 :4.8
引用产品: MCF 10A 细胞 , HCC1806 细胞 , HCC1937 细胞 , MDA-MB-468 细胞 , BT-20 细胞 , Hs 578T 细胞 , MDA-MB-436 细胞 , MCF 10A细胞专用培养基 , HCC1806细胞专用培养基 , HCC1937细胞专用培养基 , MDA-MB-468细胞专用培养基 (L15) , BT-20细胞专用培养基 , Hs 578T细胞专用培养基 , MDA-MB-436细胞专用培养基 (L15)
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Inhibitory impact of cinobufagin in triple-negative breast cancer metastasis: involvements of macrophage reprogramming through upregulated MME and inactivated FAK/STAT3 signaling (2024-01-26)
期刊:Clinical Breast Cancer
DOI:10.1016/j.clbc.2024.01.014
影响因子 :3.1
引用产品: MDA-MB-231 细胞 , MDA-MB-468 细胞 , 4T1 细胞
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Zinc finger protein 468 up-regulation of TFAM contributes to the malignant growth and cisplatin resistance of breast cancer cells (2024-03-01)
期刊:Cell Division
DOI:10.1186/s13008-024-00113-1
影响因子 :2.3
引用产品: MCF7 [MCF-7] 细胞 , MDA-MB-231 细胞 , MDA-MB-468 细胞 , DMEM/F12 培养基 , MEM (含NEAA) 培养基 , Leibovitz's L-15 培养基 , 青霉素-链霉素溶液 (双抗),100× , Annexin V-APC/ PI荧光双染细胞凋亡检测试剂盒
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A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer (2023-07-05)
期刊:Molecular Cancer
DOI:10.1186/s12943-023-01806-x
影响因子 :37.3
引用产品: MDA-MB-231 细胞 , MDA-MB-468 细胞 , 293T [HEK-293T] 细胞 , Hs 578T 细胞 , MCF7 [MCF-7] 细胞 , T-47D 细胞 , ZR-75-1 细胞 , MCF 10A 细胞
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Highly sensitive surface-enhanced Raman scattering (SERS) imaging for phenotypic diagnosis and therapeutic evaluation of breast cancer (2023-01-18)
期刊:CHEMICAL ENGINEERING JOURNAL
影响因子 :15.1
引用产品: MCF 10A 细胞 , KPL-4 细胞 , SK-BR-3 [SKBR3] 细胞 , MDA-MB-468 细胞
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Antitumor effect of CAR-T cells targeting transmembrane tumor necrosis factor alpha combined with PD-1 mAb on breast cancers (2023-01-01)
期刊:Journal for ImmunoTherapy of Cancer
影响因子 :10.9
引用产品: MDA-MB-468 细胞
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NUCB2/Nesfatin-1 drives breast cancer metastasis through the up-regulation of cholesterol synthesis via the mTORC1 pathway (2023-06-05)
期刊:Journal of Translational Medicine
DOI:10.1186/s12967-023-04236-x
影响因子 :7.4
引用产品: BT-549 细胞 , MDA-MB-231 细胞 , MDA-MB-468 细胞 , MCF7 [MCF-7] 细胞 , MCF 10A 细胞 , RPMI-1640 培养基 , 特级胎牛血清 , 重组人胰岛素溶液 (10mg/mL) , DMEM高糖 培养基
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Design, synthesis, activity and molecular dynamics studies of 1,3,4-thiadiazole derivatives as selective allosteric inhibitors of SHP2 for the treatment of cancer (2023-06-24)
期刊:EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
DOI:10.1016/j.ejmech.2023.115585
影响因子 :6.7
引用产品: MV-4-11 细胞 , NCI-H358 细胞 , A549 [A-549] 细胞 , MDA-MB-468 细胞
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Performance-enhanced clogging-free viscous sheath constriction impedance flow cytometry (2023-04-15)
期刊:LAB ON A CHIP
影响因子 :6.1
引用产品: MCF7 [MCF-7] 细胞 , MDA-MB-468 细胞 , Hep G2 细胞
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DEPDC1B-mediated USP5 deubiquitination of β-catenin promotes breast cancer metastasis by activating the wnt/β-catenin pathway (2023-08-29)
期刊:AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
DOI:10.1152/ajpcell.00249.2023
影响因子 :5.5
引用产品: MDA-MB-231 细胞 , MDA-MB-468 细胞 , MDA-MB-157 细胞 , BT-549 细胞 , 293 [HEK-293] 细胞 , DMEM/F12 培养基 , Hs 578T细胞专用培养基
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Icariin Induces Triple-Negative Breast Cancer Cell Apoptosis and Suppresses Invasion by Inhibiting the JNK/c-Jun Signaling Pathway (2023-03-28)
期刊:Drug Design Development and Therapy
影响因子 :4.8
引用产品: Hs 578T 细胞 , MDA-MB-468 细胞 , MCF 10A 细胞 , Hs 578T细胞专用培养基 , MDA-MB-468细胞专用培养基 (DMEM) , MCF 10A细胞专用培养基
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Upregulation of the ferroptosis-related STEAP3 gene is a specific predictor of poor triple-negative breast cancer patient outcomes (2023-03-31)
期刊:Frontiers in Oncology
影响因子 :4.7
引用产品: MCF 10A 细胞 , MDA-MB-231 细胞 , BT-549 细胞 , MDA-MB-468 细胞 , MCF7 [MCF-7] 细胞 , T-47D 细胞 , BT-474 [BT474] 细胞
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Gankyrin inhibits ferroptosis through the p53/SLC7A11/GPX4 axis in triple-negative breast cancer cells (2023-12-08)
期刊:Scientific Reports
DOI:10.1038/s41598-023-49136-8
影响因子 :4.6
引用产品: MDA-MB-231 细胞 , HCC1937 细胞 , MDA-MB-468 细胞 , BT-20 细胞 , Hs 578T 细胞 , MCF 10A 细胞
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Analysis of m7G-Related signatures in the tumour immune microenvironment and identification of clinical prognostic regulators in breast cancer (2023-06-23)
期刊:BMC CANCER
DOI:10.1186/s12885-023-11012-z
影响因子 :3.8
引用产品: MDA-MB-231 细胞 , MDA-MB-468 细胞 , SK-BR-3 [SKBR3] 细胞 , MCF 10A 细胞 , SK-BR-3 [SKBR3]细胞专用培养基 , MCF 10A细胞专用培养基
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Isopimaric acid, an ion channel regulator, regulates calcium and oxidative phosphorylation pathways to inhibit breast cancer proliferation and metastasis (2023-02-06)
期刊:TOXICOLOGY AND APPLIED PHARMACOLOGY
DOI:10.1016/j.taap.2023.116415
影响因子 :3.8
引用产品: MDA-MB-231 细胞 , MCF7 [MCF-7] 细胞 , MDA-MB-468 细胞 , HCC1937 细胞 , MDA-MB-453 细胞 , 4T1 细胞 , MCF 10A 细胞 , Annexin V-FITC/ PI荧光双染细胞凋亡检测试剂盒
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The combined effect and mechanism of antiangiogenic drugs and PD-L1 inhibitor on cell apoptosis in triple negative breast cancer (2023-01-31)
期刊:Annals of Translational Medicine
影响因子 :3.6
引用产品: MDA-MB-468 细胞 , BT-549 细胞
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TTK inhibition activates STING signal and promotes anti-PD1 immunotherapy in breast cancer (2023-12-18)
期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI:10.1016/j.bbrc.2023.149388
影响因子 :3.1
引用产品: 4T1 细胞 , 293T [HEK-293T] 细胞 , MDA-MB-231 细胞 , MDA-MB-468 细胞 , BT-549 细胞 , BT-474 [BT474] 细胞 , MCF7 [MCF-7] 细胞
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Identification of TFR2 as a novel ferroptosis‑related gene that serves an important role in prognosis and progression of triple‑negative breast cancer (2023-12-05)
期刊:Oncology Letters
影响因子 :2.9
引用产品: MCF 10A 细胞 , T-47D 细胞 , SK-BR-3 [SKBR3] 细胞 , BT-549 细胞 , MDA-MB-468 细胞 , MDA-MB-436 细胞 , MDA-MB-231 细胞 , 马血清 , MEM非必需氨基酸溶液 (NEAA),100×
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β-eudesmol inhibits cell proliferation and induces ferroptosis via regulating MAPK signaling pathway in breast cancer (2023-11-27)
期刊:TOXICON
DOI:10.1016/j.toxicon.2023.107529
影响因子 :2.8
引用产品: MDA-MB-468 细胞 , MDA-MB-231 细胞 , DMEM高糖 培养基 , 青霉素-链霉素溶液 (双抗),100×
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GLIS Family Zinc Finger 3 Promotes Triple-Negative Breast Cancer Progression by Inducing Cell Proliferation, Migration and Invasion, and Activating the NF-κB Signaling Pathway (2023-02-01)
期刊:BIOLOGICAL & PHARMACEUTICAL BULLETIN
影响因子 :2.0
引用产品: MDA-MB-468 细胞 , Leibovitz's L-15 培养基
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FEZF2 inhibits the growth of triple-negative breast cancer cells through EZH2/PD-L1 and enhances anti-tumor immunity in vivo (2023-06-19)
期刊:Molecular & Cellular Toxicology
DOI:10.1007/s13273-023-00368-9
影响因子 :1.7
引用产品: MCF 10A 细胞 , DMEM/F12 培养基 , 马血清 , 青霉素-链霉素溶液 (双抗),100× , MDA-MB-231 细胞 , MDA-MB-468 细胞 , DMEM高糖 培养基 , BT-549 细胞 , RPMI-1640 培养基 , MDA-MB-436 细胞
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Hsa-miR-497-3p impedes the proliferation and invasion of triple-negative breast cancer cells by controlling epithelial-mesenchymal transition through ZEB1 targeting (2023-08-31)
期刊:CELLULAR AND MOLECULAR BIOLOGY
影响因子 :1.6
引用产品: MDA-MB-231 细胞 , BT-549 细胞 , MDA-MB-468 细胞 , MCF7 [MCF-7] 细胞
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Expression of LINC00461 in breast cancer cells and its modulatory effect on miR-607/SLCLA3 axis (2023-05-11)
期刊:TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
影响因子 :0.6
引用产品: MCF 10A 细胞 , MDA-MB-468 细胞 , T-47D 细胞 , MCF7 [MCF-7] 细胞 , MDA-MB-231 细胞 , DMEM/F12 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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CKAP2L, transcriptionally inhibited by FOXP3, promotes breast carcinogenesis through the AKT/mTOR pathway (2001-02-20)
期刊:EXPERIMENTAL CELL RESEARCH
DOI:10.1016/j.yexcr.2022.113035
影响因子 :3.7
引用产品: SK-BR-3 [SKBR3] 细胞 , T-47D 细胞 , MDA-MB-231 细胞 , MDA-MB-468 细胞 , Leibovitz's L-15 培养基 , McCoy's 5A 培养基
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Deciphering the map of METTL14-mediated lncRNA m6A modification at the transcriptome-wide level in breast cancer (2022-11-06)
期刊:JOURNAL OF CLINICAL LABORATORY ANALYSIS
影响因子 :3.1
引用产品: MCF 10A 细胞 , MDA-MB-468 细胞 , MCF7 [MCF-7] 细胞 , SK-BR-3 [SKBR3] 细胞 , MDA-MB-231 细胞 , BT-474 [BT474] 细胞
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Up-regulated circBACH2 contributes to cell proliferation, invasion, and migration of triple-negative breast cancer (2004-01-20)
期刊:Cell Death & Disease
DOI:10.1038/s41419-021-03684-x
影响因子 :9.0
引用产品: MDA-MB-231 细胞 , MDA-MB-468 细胞 , MDA-MB-453 细胞 , BT-549 细胞 , MCF 10A 细胞 , MCF7 [MCF-7] 细胞 , BT-474 [BT474] 细胞
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MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis (2004-01-20)
期刊:Cell Death & Disease
DOI:10.1038/s41419-021-03661-4
影响因子 :9.0
引用产品: MDA-MB-157 细胞 , Hs 578T 细胞 , BT-549 细胞 , MDA-MB-231 细胞 , MDA-MB-468 细胞 , MCF 10A 细胞 , 293T [HEK-293T] 细胞 , DMEM/F12完全培养基 (含10%FBS)
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The curcumin analog EF24 inhibits proliferation and invasion of triple-negative breast cancer cells by targeting the lncRNA HCG11/Sp1 axis | Molecular and Cellular Biology (2011-01-20)
期刊:Molecular And Cellular Biology
影响因子 :5.3
引用产品: MDA-MB-231 细胞 , MDA-MB-468 细胞 , BT-549 细胞
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Circular RNA circ-ERBB2 Elevates the Warburg Effect and Facilitates Triple-Negative Breast Cancer Growth by the MicroRNA 136-5p/Pyruvate Dehydrogenase Kinase 4 Axis | Molecular and Cellular Biology (2009-01-20)
期刊:Molecular And Cellular Biology
影响因子 :5.3
引用产品: BT-549 细胞 , MDA-MB-453 细胞 , MDA-MB-468 细胞 , MDA-MB-231 细胞 , MCF 10A 细胞 , RPMI-1640 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , Leibovitz's L-15 培养基
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Sericin inhibits MDA‑MB‑468 cell proliferation via the PI3K/Akt pathway in triple‑negative breast cancer (2012-00-20)
期刊:Molecular Medicine Reports
影响因子 :3.4
引用产品: MDA-MB-468 细胞 , MDA-MB-453 细胞 , MCF7 [MCF-7] 细胞 , MCF 10A 细胞
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