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产品概述
名称 | NCI-H295R (人肾上腺皮质腺癌细胞) (STR鉴定正确) |
别称 | NCI H295R; NCIH295R; H295R; H-295R; H295R-S1 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | NCI-H295R细胞是源于多能肾上腺皮质癌细胞系NCI-H295细胞,后者由Gazdar·A·F等建立,从肾上腺皮质的肿瘤中分离而来。NCI-H295细胞经改变培养条件获得了NCI-295R细胞,群体倍增时间从原来的5天减为2天。NCI-295细胞悬浮生长,而NCI-H295R细胞呈单层贴壁生长。NCI-H295R细胞保留了产生雄激素的能力,对血管紧张素Ⅱ和钾离子有反应。 |
年龄(性别) | 女性;48岁 |
组织来源 | 器官:肾上腺 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肾上腺皮质癌细胞 |
生物安全等级 | BSL-1 |
基因表达情况 | aldosterone; cortisol; C19 steroids |
保藏机构 | ATCC; CRL-2128 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10,12 D2S1338 25 D3S1358 15,16 D5S818 12 D7S820 9,12 D8S1179 13 D13S317 13 D16S539 11 D18S51 17 D19S433 13 D21S11 32.2 FGA 19.2,24 PentaD 8 PentaE 5,12 TH01 9.3 TPOX 8 vWA 17,18 D6S1043 11 D12S391 19,20 D2S441 11 -
STR鉴定图
参考文献
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Sinapine Targeting PLCβ3 EF Hands Disrupts Gαq-PLCβ3 Interaction and Ameliorates Cardiovascular Diseases (2023-11-15)
作者:Simeng Chu:1.State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin, 300353, China. ; Fukui Shen:1.State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin, 300353, China. ; Wenjuan Liu:1.State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin, 300353, China. ; Jin Zhang:1.State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin, 300353, China. ; Xiaoying Wang:1.Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Tianjin, 30161
期刊:Sinapine Targeting PLCβ3 EF Hands Disrupts Gαq-PLCβ3 Interaction and Ameliorates Cardiovascular Diseases
DOI:10.1016/j.phymed.2023.155200
影响因子 :7.9
引用产品: NCI-H295R 细胞 , H9c2 (2-1) 细胞 , A7r5 细胞
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Spatial lipidomics and metabolomics of multicellular tumor spheroids using MALDI-2 and trapped ion mobility imaging (2023-06-20)
作者:Jing Chen:1.Ministry of Education Key Laboratory of Analytical Science for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China. ; Peisi Xie:1.Ministry of Education Key Laboratory of Analytical Science for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China. ; Qingyuan Dai:1.State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong SAR, China. ; Pengfei Wu:1.State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong SAR, China; College of Forestry, Nanjing Forestry University, Nanjing, Jiangsu, 210018, China. ; Yu He:1.Ministry of Education Key Laboratory of Analytical Science for Food Saf
期刊:Spatial lipidomics and metabolomics of multicellular tumor spheroids using MALDI-2 and trapped ion mobility imaging
DOI:10.1016/j.talanta.2023.124795
影响因子 :6.1
引用产品: NCI-H295R 细胞
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N7-methylguanosine regulatory genes well represented by METTL1 define vastly different prognostic, immune and therapy landscapes in adrenocortical carcinoma (2023-02-15)
作者:Fangshi Xu:1.Department of Urology, Second Affiliated Hospital of Xi'an Jiaotong University No. 157, West Five Road, Xi'an 710004, Shaanxi, China. ; 2.Department of Urology, Shaanxi Provincial People's Hospital No. 256, Friendship West Road, Xi'an 710068, Shaanxi, China. ; Danrui Cai:1.Department of Ophthalmology, Second Affiliated Hospital of Xi'an Jiaotong University No. 157, West Five Road, Xi'an 710004, Shaanxi, China. ; Shanshan Liu:1.Department of Rheumatology and Immunology, Second Affiliated Hospital of Xi'an Jiaotong University Xi'an, Shaanxi, China. ; Kaini He:1.Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University Xi'an, Shaanxi, China. ; Jing Chen:1.Department of Rheumatology and Immunology, Second Affiliated Hospital of Xi'an Jiaotong University Xi'an, Shaanxi, China. ; Li Qu:1.Department of Rheumatology and Immunology, Second Affiliated Hospital of Xi'an Jiaotong University Xi'an, Shaanxi, China. ; Tie Chong:1.Department of Urology,
期刊:N7-methylguanosine regulatory genes well represented by METTL1 define vastly different prognostic, immune and therapy landscapes in adrenocortical carcinoma
DOI:PMID:36895966
影响因子 :5.3
引用产品: NCI-H295R 细胞 , SW-13 细胞
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KIF11 is a potential prognostic biomarker and therapeutic target for adrenocortical carcinoma (2023-03-20)
作者:Yan Zhou:1.Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, China. ; Xiang Chen:1.Department of Urology, Xiangya Hospital, Central South University, Changsha, China. ; Bingsheng Li:1.Department of Urology, Xiangya Hospital, Central South University, Changsha, China. ; Yang Li:1.Department of Urology, Xiangya Hospital, Central South University, Changsha, China. ; Bo Zhang:1.Department of Urology, Xiangya Hospital, Central South University, Changsha, China. ;
期刊:KIF11 is a potential prognostic biomarker and therapeutic target for adrenocortical carcinoma
影响因子 :2.0
引用产品: NCI-H295R 细胞 , DMEM高糖 培养基
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CDK1 serves as a therapeutic target of adrenocortical carcinoma via regulating epithelial–mesenchymal transition, G2/M phase transition, and PANoptosis (2022-10-02)
作者:Liwen Ren:1.The State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing, 100050, China. ; 2.Key Laboratory of Drug Target Research and Drug Screen, Institute of Materia Medica, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100050, China. ; Yihui Yang:1.The State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing, 100050, China. ; 2.Key Laboratory of Drug Target Research and Drug Screen, Institute of Materia Medica, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100050, China. ; Wan Li:1.The State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing, 100050, China. ; 2.Key Laboratory of Drug Target Research and Drug Screen, Institute of Materia Medica, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100050, China. ; Xiangjin Zheng:1.The State Key Laboratory of Bioactive Substance and Function of Natural M
期刊:CDK1 serves as a therapeutic target of adrenocortical carcinoma via regulating epithelial–mesenchymal transition, G2/M phase transition, and PANoptosis
DOI:10.1186/s12967-022-03641-y
影响因子 :8.4
引用产品: NCI-H295R 细胞 , SW-13 细胞 , SW-13细胞专用培养基 (L15) , NCI-H295R细胞专用培养基
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Combining virtual screening and in vitro evaluation for the discovery of potential CYP11B2 inhibitors (2022-08-01)
作者:Jiali Li:1.School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China. ; 2.Key Laboratory of Screening and Activity Evaluation of Targeted Drugs, Chongqing, 400054, China. ; Na Yu:1.School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China. ; 2.Key Laboratory of Screening and Activity Evaluation of Targeted Drugs, Chongqing, 400054, China. ; Hongmei Guo:1.School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China. ; 2.Key Laboratory of Screening and Activity Evaluation of Targeted Drugs, Chongqing, 400054, China. ; YuanZe Shi:1.School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China. ; 2.Key Laboratory of Screening and Activity Evaluation of Targeted Drugs, Chongqing, 400054, China. ; XiaoDie Chen:1.School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China. ; 2.Key Laboratory
期刊:Combining virtual screening and in vitro evaluation for the discovery of potential CYP11B2 inhibitors
影响因子 :4.8
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IFNγ enhances ferroptosis by increasing JAK‑STAT pathway activation to suppress SLCA711 expression in adrenocortical carcinoma (2003-02-20)
作者:Xinbo Yu:1.Department of Urology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China. ; Dandan Zhu:1.Department of Urology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China. ; Bixian Luo:1.Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China. ; Wei Kou:1.Department of Urology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China. ; Yuling Cheng:1.Department of Urology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China. ; Yu Zhu:1.Department of Urology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China. ;
期刊:IFNγ enhances ferroptosis by increasing JAK‑STAT pathway activation to suppress SLCA711 expression in adrenocortical carcinoma
影响因子 :4.2
引用产品: NCI-H295R 细胞 , NCI-H295R细胞专用培养基
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Identification of Seven Aberrantly Methylated and Expressed Genes in Adrenocortical Carcinoma (2007-09-20)
作者:He Xiao:1.Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China. ; Weixiang He:1.Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China. ; Ping Chen:1.Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China. ; Deqiang Xu:1.Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China. ; Guang Zeng:1.Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China. ; Zhuo Li:1.Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China. ; 2.Department of Urology, Shenzhen Sixth People's Hospital, Shenzhen, China. ; 3.The Sixth Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China. ; 4.Affiliated Shenzhen Sixth Hospital of Guangdong Medical University, Shenzhen, China. ; 5.Shenzhen Key Laboratory for Endogenous Infection, Shenzhen, China. ; Mingliu Huang:1.Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China. ; Xinghuan Wang:1.Departme
期刊:Identification of Seven Aberrantly Methylated and Expressed Genes in Adrenocortical Carcinoma
影响因子 :5.2
引用产品: SW-13 细胞 , NCI-H295R 细胞
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