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产品概述
| 名称 | WPMY-1 (人正常前列腺基质永生化细胞) (STR鉴定正确) |
| 别称 | WPMY1 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成肌细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
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| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | 肌成纤维基质细胞株，WPMY-1细胞与RWPE-1细胞一样，来源于同一张成人前列腺组织切片的周围区域的基质细胞。通过一个pRSTV质料结构，用SV40大T抗原对基质细胞进行永生化。WPMY-1细胞与RWPE-1细胞及其它上皮细胞衍生株一样，属于来源于同一个前列腺的一系列细胞株。由于WPMY-1细胞与RWPE-1细胞来源于同一个前列腺的周围区域，WPMY-1细胞株对于研究分泌和基质与上皮细胞相互作用尤其有用。据提供者报道，来源于同一个前列腺的RWPE-1细胞株，经过检测，乙肝、丙肝、人免疫缺陷病毒都呈阴性。 |
| 年龄（性别） | 男性；54岁 |
| 组织来源 | 前列腺/基质 |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | BSL-2 |
| 保藏机构 | ATCC; CRL-2854 |
STR鉴定
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STR位点信息
Amelogenin X, Y CSF1PO 13 D2S1338 17,20 D3S1358 15,16 D5S818 12,15 D7S820 10,11 D8S1179 10,14 D13S317 8,14 D16S539 9 D18S51 14,16 D19S433 13 D21S11 29,31 FGA 24,25 PentaD 10,13 PentaE 5 TH01 8,9.3 TPOX 8,11 vWA 14,18 D6S1043 18,19 D12S391 20,23 D2S441 11 -
STR鉴定图
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参考文献
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Dual deficiency of melatonin and dihydrotestosterone promotes stromal cell damage and mediates prostatitis via the cGAS-STING pathway in sleep-deprived mice (2024-03-15)
作者：Jia Chen:1.Department of Urology, The First Affiliated Hospital of Anhui Medical University, Institute of Urology, Anhui Medical University, Anhui Province Key Laboratory of Genitourinary Diseases, Hefei, 230022, P.R. China. ; Wenming Ma:1.Department of Urology, The First Affiliated Hospital of Anhui Medical University, Institute of Urology, Anhui Medical University, Anhui Province Key Laboratory of Genitourinary Diseases, Hefei, 230022, P.R. China. ; Shaoyu Yue:1.Department of Urology, The First Affiliated Hospital of Anhui Medical University, Institute of Urology, Anhui Medical University, Anhui Province Key Laboratory of Genitourinary Diseases, Hefei, 230022, P.R. China. ; Dongsheng Li:1.Department of Urology, The First Affiliated Hospital of Anhui Medical University, Institute of Urology, Anhui Medical University, Anhui Province Key Laboratory of Genitourinary Diseases, Hefei, 230022, P.R. China. ; Lei Chen:1.Department of Urology, The First Affiliated Hospital of Anhui Medical
期刊：Dual deficiency of melatonin and dihydrotestosterone promotes stromal cell damage and mediates prostatitis via the cGAS-STING pathway in sleep-deprived mice
DOI：10.1186/s12964-024-01554-5
影响因子 ：8.4
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Triggering pyroptosis enhances the antitumor efficacy of PARP inhibitors in prostate cancer (2023-08-23)
作者：Ao Tian:1.State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine and School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, China. ; Tingyu Wu:1.State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine and School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, China. ; Yanshuang Zhang:1.State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine and School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, China. ; Jiachen Chen:1.State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine and School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, China. ; Jianjun Sha:1.Department of Urology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 145 Shandong Middle road
期刊：Triggering pyroptosis enhances the antitumor efficacy of PARP inhibitors in prostate cancer
DOI：10.1007/s13402-023-00860-3
影响因子 ：6.6
引用产品： LNCaP clone FGC 细胞 , WPMY-1 细胞
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Analyzing roles of small nucleolar RNA host gene 25 from clinical, molecular target and tumor formation in prostate cancer (2023-06-10)
作者：Zelin Liu:1.Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, China; Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430074, China. ; Shuai Ke:1.Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, China. ; Qinghua Wang:1.Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, China. ; Xuhang Gu:1.School of Minerals Processing and Bioengineering, Central South University, Changsha, 410000, China. ; Guanzhong Zhai:1.Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, China. ; Haoren Shao:1.Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, China. ; Mu He:1.Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, China. ; Jia Guo:1.Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, China. Electronic address: doctor_zelin@hust.edu
期刊：Analyzing roles of small nucleolar RNA host gene 25 from clinical, molecular target and tumor formation in prostate cancer
DOI：10.1016/j.yexcr.2023.113686
影响因子 ：3.7
引用产品： PC-3 细胞 , LNCaP clone FGC 细胞 , DU 145 细胞 , WPMY-1 细胞
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TNS1 regulated by HLF represses the progression of prostate cancer (2023-09-15)
作者：Hao Zhou:1.Department of Urology, The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410001, Hunan, P.R. China. ; Fang Wang:1.Medical College, Changsha Social Work College, Changsha 410004, Hunan, P.R. China. ;
期刊：TNS1 regulated by HLF represses the progression of prostate cancer
影响因子 ：2.7
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Juglone promotes antitumor activity against prostate cancer via suppressing glycolysis and oxidative phosphorylation (2022-10-24)
作者：Cheng Hu:1.College of Laboratory Medicine, Jilin Medical University, Jilin City, Jilin, People's Republic of China. ; Haiyue Xu:1.Clinical Laboratory Department, Changchun Obstetrics and Gynecology Hospital, Changchun city, Jilin, People's Republic of China. ; Zehao Li:1.College of Laboratory Medicine, Jilin Medical University, Jilin City, Jilin, People's Republic of China. ; Dandan Liu:1.College of Laboratory Medicine, Jilin Medical University, Jilin City, Jilin, People's Republic of China. ; Siqi Zhang:1.College of medical technology, Beihua university, Jilin City, Jilin, People's Republic of China. ; Fang Fang:1.College of Laboratory Medicine, Jilin Medical University, Jilin City, Jilin, People's Republic of China. ; Liguo Wang:1.Department of Urology Surgery, Affiliated Hospital of Jilin Medical University, Jilin City, Jilin, People's Republic of China. ;
期刊：Juglone promotes antitumor activity against prostate cancer via suppressing glycolysis and oxidative phosphorylation
DOI：10.1002/ptr.7631
影响因子 ：6.4
引用产品： WPMY-1 细胞
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