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产品概述
名称 | HBE135-E6E7 (人支气管上皮细胞) (STR鉴定正确) |
别称 | HBE 135-E6E7; HBE 135-E6/E7 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样;卵圆形 |
冻存条件 | 冻存液:55%DM/F12+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | The HBE135-E6E7 cell line was derived from normal bronchial epithelium taken from a man undergoing lobectomy for squamous cell carcinoma. Cells from the primary explant in their first passage were infected with the recombinant retrovirus LXSN16E6E7 containing the human papilloma virus (HPV) E6E7 gene. Cells were selected in the presence of 0.4 mg/mL G418 |
年龄(性别) | 男性,;54岁 |
组织来源 | 肺;支气管 |
细胞类型 | 转化细胞系 |
生物安全等级 | BSL-2 |
倍增时间 | 24-30 hours |
保藏机构 | ATCC; CRL-2741 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 9,11 D2S1338 16,17 D3S1358 15,17 D5S818 12 D7S820 9,10 D8S1179 13,14 D13S317 8,11 D16S539 9,12 D18S51 12 D19S433 13,16 D21S11 28,30 FGA 24,25 PentaD 9,12 PentaE 12,16 TH01 5,6 TPOX 8,10 vWA 16,17 D6S1043 13 D12S391 19,24 D2S441 10,11 -
STR鉴定图
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参考文献
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Comprehensive analysis of BUBs gene family in lung adenocarcinoma with immunological analysis (2023-02-13)
期刊:Aging-US
影响因子 :5.2
引用产品: HBE135-E6E7 细胞
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Hsa_circ_0003220 Drives Chemoresistance of Human NSCLC Cells by Modulating miR-489-3p/IGF1 (2023-06-16)
期刊:International Journal of Genomics
影响因子 :2.9
引用产品: 293T [HEK-293T] 细胞 , NCI-H460 [H460] 细胞 , A549 [A-549] 细胞 , HBE135-E6E7 细胞
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Hsa_circ_0003489 Drives PTX Resistance of Human NSCLC Cells Through Modulating miR-98-5p/IGF2 (2023-09-04)
期刊:Pharmacogenomics & Personalized Medicine
影响因子 :1.9
引用产品: NCI-H460 [H460] 细胞 , A549 [A-549] 细胞 , HBE135-E6E7 细胞
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Low-density lipoprotein receptor-related protein 8 facilitates the proliferation and invasion of non-small cell lung cancer cells by regulating the Wnt/β-catenin signaling pathway (2003-02-20)
期刊:Bioengineered
DOI:10.1080/21655979.2022.2036917
影响因子 :4.9
引用产品: HBE135-E6E7 细胞
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Hsa_circ_0017639 regulates cisplatin resistance and tumor growth via acting as a miR-1296-5p molecular sponge and modulating sine oculis homeobox 1 expression in non-small cell lung cancer (2003-02-20)
期刊:Bioengineered
DOI:10.1080/21655979.2022.2053810
影响因子 :4.9
引用产品: 293T [HEK-293T] 细胞 , NCI-H460 [H460] 细胞 , NCI-H1650 细胞 , A549 [A-549] 细胞 , NCI-H1299 细胞 , HBE135-E6E7 细胞
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Lidocaine represses the malignant behavior of lung carcinoma cells via the circ_PDZD8/miR-516b-5p/GOLT1A axis (2001-02-20)
期刊:Histology And Histopathology
影响因子 :2.0
引用产品: HBE135-E6E7 细胞
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FERMT3 mediates cigarette smoke-induced epithelial–mesenchymal transition through Wnt/β-catenin signaling (2011-01-20)
期刊:Respiratory Research
DOI:10.1186/s12931-021-01881-y
影响因子 :7.2
引用产品: A549 [A-549] 细胞 , HBE135-E6E7 细胞
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Vitamin E relieves chronic obstructive pulmonary disease by inhibiting COX2-mediated p-STAT3 nuclear translocation through the EGFR/MAPK signaling pathway (2011-01-20)
期刊:Laboratory Investigation
DOI:10.1038/s41374-021-00652-z
影响因子 :5.5
引用产品: HBE135-E6E7 细胞
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Circ_0017956 promotes the proliferation and metastasis of non-small cell lung cancer through regulating miR-515-5p/ITGB8 axis (2005-01-20)
期刊:Cell Cycle
DOI:10.1080/15384101.2021.1919829
影响因子 :4.3
引用产品: A549/DDP 细胞 , NCI-H1299 细胞 , HBE135-E6E7 细胞
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MUC13 promotes lung cancer development and progression by activating ERK signaling (2012-01-20)
期刊:Oncology Letters
影响因子 :2.9
引用产品: HBE135-E6E7 细胞
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A novel EZH2/NXPH4/CDKN2A axis is involved in regulating the proliferation and migration of non-small cell lung cancer cells (2012-01-20)
期刊:Bioscience Biotechnology and Biochemistry
影响因子 :1.6
引用产品: HBE135-E6E7 细胞
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N-Butanol Fraction of Wenxia Formula Extract Inhibits the Growth and Invasion of Non-Small Cell Lung Cancer by Down-Regulating Sp1-Mediated MMP2 Expression (2011-00-20)
期刊:Frontiers in Pharmacology
影响因子 :5.6
引用产品: HBE135-E6E7 细胞
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Inhibition of SNCG suppresses the proliferation of lung cancer cells induced by high glucose (2012-00-20)
期刊:Molecular Medicine Reports
影响因子 :3.4
引用产品: HBE135-E6E7 细胞
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Luminescent ruthenium(II) polypyridyl complexes acted as radiosensitizer for pancreatic cancer by enhancing radiation-induced DNA damage (2009-09-20)
期刊:Theranostics
影响因子 :12.4
引用产品: HBE135-E6E7 细胞
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Thymic stromal lymphopoietin induced early stage of epithelial-mesenchymal transition in human bronchial epithelial cells through upregulation of transforming growth factor beta 1 (2008-09-20)
期刊:Experimental Lung Research
DOI:10.1080/01902148.2019.1646841
影响因子 :1.7
引用产品: HBE135-E6E7 细胞
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LncRNA CCAT2 promotes tumorigenesis by over-expressed Pokemon in non-small cell lung cancer (2001-07-20)
期刊:Biomedicine & Pharmacotherapy
DOI:10.1016/j.biopha.2016.12.122
影响因子 :7.5
引用产品: PC-9 细胞 , NCI-H358 细胞 , NCI-H1975 细胞 , HBE135-E6E7 细胞
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