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产品概述
名称 | Calu-3 (人肺腺癌细胞(胸水)) (STR鉴定正确) |
别称 | CaLu-3; CALU-3; Calu 3; Calu3; CALU3 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞较难消化,注意延长消化时间,消化到细胞收缩变圆,轻拍培养瓶侧边,细胞可以滑落方可终止消化。 |
背景描述 | Calu-3细胞是从一名25岁的白人男性肺腺癌患者的胸水中分离建系的;该患者曾使用过环磷酰胺、博来霉素、阿霉素进行治疗。Calu-3细胞接种至裸鼠可成瘤;Calu-3细胞亦可作转染宿主。 |
年龄(性别) | 男性;25岁 |
组织来源 | 肺;转移灶;胸水腺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~35-45 hours |
致瘤性 | Yes, forms well differentiated grade I adenocarcinoma in nude mice. |
抗原表达情况 | Blood Type A; Rh+ |
基因表达情况 | Blood Type A; Rh+ |
保藏机构 | ATCC; HTB-55 BCRJ; 0264 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11,12 D2S1338 19 D3S1358 15,18 D5S818 11 D7S820 10,11 D8S1179 11,15 D13S317 12 D16S539 12,14 D18S51 14 ,17 D19S433 12,13 D21S11 28,30 FGA 25 PentaD 9,16 PentaE 5,21 TH01 6,9.3 TPOX 8 vWA 16,17 D6S1043 11 D12S391 21,22 D2S441 14 -
STR鉴定图
参考文献
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Targeting TYK2 alleviates Rab27A-induced malignant progression of non-small cell lung cancer via disrupting IFNα-TYK2-STAT-HSPA5 axis (2024-03-23)
作者:Yuanyuan Zeng:1.Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, 215006, Suzhou, China. ; 2.Institute of Respiratory Diseases, Soochow University, 215006, Suzhou, China. ; 3.Suzhou Key Laboratory for Respiratory Diseases, 215006, Suzhou, China. ; Jian Zhao:1.Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, 215006, Suzhou, China. ; Zhengyan Wu:1.Department of Health Management Center, The First Affiliated Hospital of Soochow University, Suzhou, 215006, Suzhou, China. ; Yongkang Huang:1.Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, 215006, Suzhou, China. ; Anqi Wang:1.Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, 215006, Suzhou, China. ; Jianjie Zhu:1.Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, 21
期刊:Targeting TYK2 alleviates Rab27A-induced malignant progression of non-small cell lung cancer via disrupting IFNα-TYK2-STAT-HSPA5 axis
DOI:10.1038/s41698-024-00574-1
影响因子 :7.9
引用产品: 293T [HEK-293T] 细胞 , BEAS-2B 细胞 , NCI-H1299 细胞 , A549 [A-549] 细胞 , LTEP-α-2 细胞 , NCI-H226 [H226] 细胞 , Calu-3 细胞 , HCC827 细胞 , NCI-H1975 细胞
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Activation of Hypoxia Inducible Factor-1 Alpha-Mediated DNA Methylation Enzymes (DNMT3a and TET2) Under Hypoxic Conditions Regulates S100A6 Transcription to Promote Lung Cancer Cell Growth and Metastasis (2024-03-18)
作者:Tengfei Wang:1.The Department of Thoracic Surgery; Anhui Wanbei Coal-Electricity Group General Hospital, Suzhou, China. ; Genbao Zhu:1.General Clinical Research Center; Anhui Wanbei Coal-Electricity Group General Hospital, Suzhou, China. ; Bo Wang:1.The Department of Thoracic Surgery; Anhui Wanbei Coal-Electricity Group General Hospital, Suzhou, China. ; Mengxue Hu:1.General Clinical Research Center; Anhui Wanbei Coal-Electricity Group General Hospital, Suzhou, China. ; Chen Gong:1.General Clinical Research Center; Anhui Wanbei Coal-Electricity Group General Hospital, Suzhou, China. ; Kemeng Tan:1.General Clinical Research Center; Anhui Wanbei Coal-Electricity Group General Hospital, Suzhou, China. ; La Jiang:1.General Clinical Research Center; Anhui Wanbei Coal-Electricity Group General Hospital, Suzhou, China. ; Xiaohong Zhu:1.General Clinical Research Center; Anhui Wanbei Coal-Electricity Group General Hospital, Suzhou, China. ; Yuliu Geng:1.The Department of Thoracic Surger
期刊:Activation of Hypoxia Inducible Factor-1 Alpha-Mediated DNA Methylation Enzymes (DNMT3a and TET2) Under Hypoxic Conditions Regulates S100A6 Transcription to Promote Lung Cancer Cell Growth and Metastasis
影响因子 :6.6
引用产品: BEAS-2B 细胞 , Calu-3 细胞 , NCI-H1395 细胞 , NCI-H1975 细胞 , A549 [A-549] 细胞
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Synergistic inhibition effects of andrographolide and baicalin on coronavirus mechanisms by downregulation of ACE2 protein level (2024-02-21)
作者:Lina Wan:1.Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, China. ; Yuchen Li:1.Laboratory Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, China. ; Wenhao Liao:1.Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, China. ; Lizhen Lei:1.Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, China. ; Maoyuan Zhao:1.Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, China. ; Jinhao Zeng:1.TCM Regulating Metabolic Diseases Key Laboratory of Si Chuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, China. zengjinhao@cdutcm.edu.cn. ; 2.Department of Digestive, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, China. zengjinhao@cdutcm.edu.cn. ; Ziyi Zhao:1.TCM Regulating Metabolic Diseases Key Laboratory of Si Chuan Province, Hospital of Chengdu University
期刊:Synergistic inhibition effects of andrographolide and baicalin on coronavirus mechanisms by downregulation of ACE2 protein level
DOI:10.1038/s41598-024-54722-5
影响因子 :4.6
引用产品: Calu-3 细胞
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Aryl hydrocarbon receptor is a proviral host factor and a candidate pan-SARS-CoV-2 therapeutic target (2023-05-31)
作者:Jiandong Shi:1.National Kunming High-level Biosafety Primate Research Center, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. ; Tingfu Du:1.National Kunming High-level Biosafety Primate Research Center, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. ; Junbin Wang:1.National Kunming High-level Biosafety Primate Research Center, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. ; Cong Tang:1.National Kunming High-level Biosafety Primate Research Center, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. ; Mengyue Lei:1.National Kunming High-level Biosafety Primate Research Center, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. ; Wenhai Yu:1.National Ku
期刊:Aryl hydrocarbon receptor is a proviral host factor and a candidate pan-SARS-CoV-2 therapeutic target
影响因子 :13.6
引用产品: Hep G2 细胞 , HuH-7 细胞 , NCI-H1299 细胞 , BEAS-2B 细胞 , Calu-3 细胞
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Decreased Sphingosine Due to Down-Regulation of Acid Ceramidase Expression in Airway of Bronchiectasis Patients: A Potential Contributor to Pseudomonas aeruginosa Infection (2023-04-27)
作者:Qian Qi:1.Department of Respiratory, the First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Institute of Respiratory Diseases, Jinan, Shandong Province, People's Republic of China. ; 2.Department of Respiratory, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong Province, People's Republic of China. ; Jiawei Xu:1.Department of Respiratory, the First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Institute of Respiratory Diseases, Jinan, Shandong Province, People's Republic of China. ; 2.Department of Respiratory, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong Province, People's Republic of China. ; Yujiao Wang:1.Department of Clinical Laboratory Medicine, the First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, Shandong Province, People's Repub
期刊:Decreased Sphingosine Due to Down-Regulation of Acid Ceramidase Expression in Airway of Bronchiectasis Patients: A Potential Contributor to Pseudomonas aeruginosa Infection
影响因子 :3.9
引用产品: Calu-3 细胞
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Hsa_circ_0080608 Attenuates Lung Cancer Progression by Functioning as a Competitive Endogenous RNA to Regulate the miR-661/ADRA1A Pathway (2023-10-11)
作者:Chengbo Ren:1.Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China. ; Ling Cui:1.Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China. ; Ruibiao Li:1.Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China. ; Xiao Song:1.Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China. ; Jinqiu Li:1.Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China. ; Qiang Xi:1.Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China. ; Zhilin Zhang:1.Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China. ; Lixia Zhao:1.Department of Internal Medicine Oncology, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China. ;
期刊:Hsa_circ_0080608 Attenuates Lung Cancer Progression by Functioning as a Competitive Endogenous RNA to Regulate the miR-661/ADRA1A Pathway
影响因子 :2.2
引用产品: A549 [A-549] 细胞 , Calu-3 细胞 , NCI-H1975 细胞 , PC-9 细胞 , BEAS-2B 细胞 , RPMI-1640 培养基 , 特级胎牛血清 , DMEM高糖 培养基
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APOE interacts with ACE2 inhibiting SARS-CoV-2 cellular entry and inflammation in COVID-19 patients (2022-08-01)
作者:Hongsheng Zhang:1.Center for Brain Sciences, the First Affiliated Hospital of Xiamen University, Institute of Neuroscience, Fujian Provincial Key Laboratory of Neurodegenerative Disease and Aging Research, Xiamen University, Xiamen, China. ; 2.Institute for Brain Science and Disease, Chongqing Medical University, Chongqing, China. ; Lin Shao:1.School of Medicine, Xiamen University, Xiamen, China. ; Zhihao Lin:1.School of Medicine, Xiamen University, Xiamen, China. ; Quan-Xin Long:1.Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Chongqing Medical University, Chongqing, China. ; Huilong Yuan:1.School of Medicine, Xiamen University, Xiamen, China. ; Lujian Cai:1.School of Medicine, Xiamen University, Xiamen, China. ; Guangtong Jiang:1.School of Medicine, Xiamen University, Xiamen, China. ; Xiaoyi Guo:1.School of Medicine, Xiamen University, Xiamen, China. ; Renzhi Yang:1.Institute for Brain Science and Disease, Chongqing Medical University, Ch
期刊:APOE interacts with ACE2 inhibiting SARS-CoV-2 cellular entry and inflammation in COVID-19 patients
DOI:10.1038/s41392-022-01118-4
影响因子 :38.1
引用产品: Calu-3 细胞
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Characteristics of replication and pathogenicity of SARS-CoV-2 Alpha and Delta isolates (2022-09-24)
作者:Xiao-Li Feng:1.Kunming National High-level Biosafety Research Center for Non-Human Primates, Center for Biosafety Mega-Science, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650107, China. ; Dandan Yu:1.Kunming National High-level Biosafety Research Center for Non-Human Primates, Center for Biosafety Mega-Science, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650107, China; Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, and KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650201, China; National Resource Center for Non-Human Primates, National Research Facility for Phenotypic & Genetic Analysis of Model Animals (Primate Facility), Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650107, China. ; Mi Zhang:1.Department of Infectious Diseases, Yunnan Provincial Infectious D
期刊:Characteristics of replication and pathogenicity of SARS-CoV-2 Alpha and Delta isolates
DOI:10.1016/j.virs.2022.09.007
影响因子 :6.9
引用产品: Calu-3 细胞
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Comprehensive Analysis of a Novel Immune-Related Gene Signature in Lung Adenocarcinoma (2022-10-19)
作者:Hongxiang Feng:1.Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing 100029, China. ; Chaoyang Liang:1.Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing 100029, China. ; Yuhui Shi:1.Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing 100029, China. ; Deruo Liu:1.Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing 100029, China. ; Jin Zhang:1.Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing 100029, China. ; Zhenrong Zhang:1.Department of Thoracic Surgery, China-Japan Friendship Hospital, Beijing 100029, China. ;
期刊:Comprehensive Analysis of a Novel Immune-Related Gene Signature in Lung Adenocarcinoma
影响因子 :5.0
引用产品: A549 [A-549] 细胞 , NCI-H1975 细胞 , Calu-3 细胞 , BEAS-2B 细胞 , MEM (含NEAA) 培养基
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The HNF4α-BC200-FMR1 positive feedback loop promotes growth and metastasis in invasive mucinous lung adenocarcinoma (2001-01-20)
作者:Xiong Chen:1.Cancer Research Center, School of Medicine, Xiamen University, Xiamen, P.R. China. ; Yujie Zhao:1.Cancer Research Center, School of Medicine, Xiamen University, Xiamen, P.R. China. ; Daxuan Wang:1.Department of Respiratory Medicine, Fujian Provincial Hospital, Fuzhou, Fujian, P.R. China. ; Ying Lin:1.Department of Pathology, Fujian Provincial Hospital, Fuzhou, Fujian, P.R. China. ; Jihuan Hou:1.Cancer Research Center, School of Medicine, Xiamen University, Xiamen, P.R. China. ; Xiaolin Xu:1.Cancer Research Center, School of Medicine, Xiamen University, Xiamen, P.R. China. ; Jianben Wu:1.Cancer Research Center, School of Medicine, Xiamen University, Xiamen, P.R. China. ; Linhai Zhong:1.Cancer Research Center, School of Medicine, Xiamen University, Xiamen, P.R. China. ; Yitong Zhou:1.Cancer Research Center, School of Medicine, Xiamen University, Xiamen, P.R. China. ; Jinying Shen:1.Cancer Research Center, School of Medicine, Xiamen University, Xiamen, P.R. China. ;
期刊:The HNF4α-BC200-FMR1 positive feedback loop promotes growth and metastasis in invasive mucinous lung adenocarcinoma
DOI:10.1158/0008-5472.CAN-21-0980
影响因子 :11.2
引用产品: Calu-3 细胞
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Downregulation of the Circadian Rhythm Regulator HLF Promotes Multiple-Organ Distant Metastases in Non-Small Cell Lung Cancer Through PPAR NF-κb Signaling (2007-00-20)
作者:Jiarong Chen:1.Clinical Experimental Center, Jiangmen Key Laboratory of Clinical Biobanks and Translational Research, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen University, Jiangmen, 529030, China; Department of Oncology, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen University, Jiangmen, 529030, China. ; Aibin Liu:1.Department of Geriatrics, Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, 410008, China. ; Zhichao Lin:1.Department of Thoracic Surgery, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen University, Jiangmen, 529030, China. ; Bin Wang:1.Clinical Experimental Center, Jiangmen Key Laboratory of Clinical Biobanks and Translational Research, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen University, Jiangmen, 529030, China;
期刊:Downregulation of the Circadian Rhythm Regulator HLF Promotes Multiple-Organ Distant Metastases in Non-Small Cell Lung Cancer Through PPAR NF-κb Signaling
DOI:10.1016/j.canlet.2020.04.007
影响因子 :9.7
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