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产品概述
| 名称 | NCI-H358 (人非小细胞肺癌细胞) (STR鉴定正确) |
| 别称 | H358; H-358; NCIH358 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
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| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | NCI-H358细胞是于1981年从一位开始化疗之前的患者的肿瘤组织中分离得到的。超微结构研究表明，NCI-H358细胞的细胞质中有Clara细胞的特征结构，NCI-H358细胞表达主要的肺表面结合蛋白SP-A和RNA，不表达SP-B和SP-C；NCI-H358细胞在软琼脂中的克隆形成效率为0.83%。 |
| 年龄（性别） | 男性 |
| 组织来源 | 细支气管及肺泡癌；非小细胞肺癌；肺；细支气管；肺泡 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肺癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~38-60 hours |
| 致瘤性 | Yes, the cells produce tumors in athymic nude mice. |
| 基因表达情况 | lung surfactant associated protein A (SP-A), The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein. |
| 保藏机构 | ATCC; CRL-5807 ECACC; 95111733 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 17,23 D3S1358 14,18 D5S818 10,12 D7S820 10,11 D8S1179 13,14 D13S317 8,12 D16S539 12,13 D18S51 14 D19S433 13,14 D21S11 28,30 FGA 20,21 PentaD 10,13 PentaE 18 TH01 6 TPOX 8,9 vWA 17 D6S1043 14 D12S391 20 D2S441 11,15 -
STR鉴定图
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参考文献
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Design, synthesis, activity and molecular dynamics studies of 1,3,4-thiadiazole derivatives as selective allosteric inhibitors of SHP2 for the treatment of cancer (2023-06-24)
作者：Wen-Shan Liu:1.Shandong Key Laboratory of Medicine and Health (Clinical Applied Pharmacology), Department of Pharmacy, Affiliated Hospital of Weifang Medical University, Weifang, 261041, Shandong Province, China; Clinical Research Center, Affiliated Hospital of Weifang Medical University, Weifang, 261041, Shandong Province, China. Electronic address: liuwenshan@wfmc.edu.cn. ; Ji-Feng Zhao:1.Shandong Key Laboratory of Medicine and Health (Clinical Applied Pharmacology), Department of Pharmacy, Affiliated Hospital of Weifang Medical University, Weifang, 261041, Shandong Province, China; Clinical Research Center, Affiliated Hospital of Weifang Medical University, Weifang, 261041, Shandong Province, China. ; Xiao-Jing Guo:1.Clinical Research Center, Affiliated Hospital of Weifang Medical University, Weifang, 261041, Shandong Province, China. ; Sheng-Ze Lu:1.School of Pharmacy, Weifang Medical University, Weifang, 261053, Shandong Province, China. ; Wei Li:1.School of Pharmacy, Weifang
期刊：Design, synthesis, activity and molecular dynamics studies of 1,3,4-thiadiazole derivatives as selective allosteric inhibitors of SHP2 for the treatment of cancer
DOI：10.1016/j.ejmech.2023.115585
影响因子 ：6.7
引用产品： MV-4-11 细胞 , NCI-H358 细胞 , A549 [A-549] 细胞 , MDA-MB-468 细胞
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Upregulation of NDUFAF2 in Lung Adenocarcinoma Is a Novel Independent Prognostic Biomarker (2023-01-17)
作者：Kang Zou:1.Department of Critical Care Medicine, Medical Center of Anesthesiology and Pain, The First Affiliated Hospital of Nanchang University, Nanchang City, Jiangxi Province 330006, China. ; 2.Department of Critical Care Medicine, The First Affiliated Hospital of Gannan Medical College, Ganzhou City, Jiangxi Province 341000, China. ; Pengxiang Gao:1.Department of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang City, Jiangxi Province 330006, China. ; Xinping Xu:1.Jiangxi Institute of Respiratory Diseases, The First Affiliated Hospital of Nanchang University, Nanchang City, Jiangxi Province 330006, China. ; 2.Jiangxi Clinical Research Center for Respiratory Diseases, The First Affiliated Hospital of Nanchang University, Nanchang City, Jiangxi Province 330006, China. ; Wei Zhang:1.Department of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang City, Jiangxi Province 330006, China. ; 2.Jiangxi Institute of Respi
期刊：Upregulation of NDUFAF2 in Lung Adenocarcinoma Is a Novel Independent Prognostic Biomarker
影响因子 ：2.8
引用产品： PC-9 细胞 , NCI-H358 细胞 , A549 [A-549] 细胞 , HCC827 细胞 , NCI-H1299 细胞 , NCI-H1975 细胞 , BEAS-2B 细胞
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Exosome-transmitted S100A4 induces immunosuppression and non-small cell lung cancer development by activating STAT3 (2022-11-12)
作者：Xu Wu:1.Department of Respiratory Medicine, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, China. ; Hui Zhang:1.Department of Respiratory Medicine, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, China. ; Gang Jiang:1.Department of Respiratory Medicine, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, China. ; Minlian Peng:1.Department of Respiratory Medicine, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, China. ; Cheng Li:1.Department of Respiratory Medicine, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, China. ; Jiaxin Lu:1.Department of Respiratory Medicine, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, China. ; Shiyin Jiang:1.Departme
期刊：Exosome-transmitted S100A4 induces immunosuppression and non-small cell lung cancer development by activating STAT3
影响因子 ：5.7
引用产品： MRC-5 细胞 , A549 [A-549] 细胞 , NCI-H358 细胞 , NCI-H1299 细胞 , NCI-H460 [H460] 细胞
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YBX1 regulated by Runx3-miR-148a-3p axis facilitates non-small-cell lung cancer progression (2005-01-20)
作者：Hongbo Su:1.Department of Pathology, First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, PR China. ; Guanzhi Fan:1.Department of Pathology, Shengjing Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, PR China. ; Jin Huang:1.Department of Radiotherapy, First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, PR China. ; Xueshan Qiu:1.Department of Pathology, First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, PR China. Electronic address: xsqiu@cmu.edu.cn. ;
期刊：YBX1 regulated by Runx3-miR-148a-3p axis facilitates non-small-cell lung cancer progression
DOI：10.1016/j.cellsig.2021.110049
影响因子 ：4.8
引用产品： BEAS-2B , HCC827 细胞 , NCI-H1650 细胞 , NCI-H1299 细胞 , NCI-H358 细胞 , A549 [A-549] 细胞 , RPMI-1640 培养基 , 人支气管上皮细胞完全培养基
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LncRNA CCAT2 promotes tumorigenesis by over-expressed Pokemon in non-small cell lung cancer (2001-07-20)
作者：Zhihong Zhao:1.Department of Thoracic Surgery, The First Affiliated Hospital of Harbin Medical University, China. Electronic address: zhihongzhao123@126.com. ; Ju Wang:1.Department of Thoracic Surgery, The First Affiliated Hospital of Harbin Medical University, China. ; Shengfa Wang:1.Department of Thoracic Surgery, The First Affiliated Hospital of Harbin Medical University, China. ; Hao Chang:1.Department of Thoracic Surgery, The First Affiliated Hospital of Harbin Medical University, China. ; Tiewa Zhang:1.Department of Thoracic Surgery, The First Affiliated Hospital of Harbin Medical University, China. ; Junfeng Qu:1.Department of Thoracic Surgery, The First Affiliated Hospital of Harbin Medical University, China. ;
期刊：LncRNA CCAT2 promotes tumorigenesis by over-expressed Pokemon in non-small cell lung cancer
DOI：10.1016/j.biopha.2016.12.122
影响因子 ：7.5
引用产品： PC-9 细胞 , NCI-H358 细胞 , NCI-H1975 细胞 , HBE135-E6E7 细胞
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