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产品概述
| 名称 | BeWo (人绒毛膜肿瘤细胞) (STR鉴定正确) |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:3 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | BeWo细胞取自人绒癌脑转移组织，在仓鼠颊囊移植传代8年。利用移植瘤组织进行体外培养，建立细胞系。利用不同传代方法建立了不同亚系，JEG-3是其衍生克隆。BeWo细胞可以产生雌激素、孕激素、雌酮、雌二醇、雌三醇、hCG、胎盘催乳素、角蛋白等。 |
| 年龄（性别） | 胚胎 |
| 组织来源 | 胎盘 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 其它肿瘤细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~30 hours |
| 保藏机构 | ATCC; CCL-98 DSMZ; ACC-458 ECACC; 86082803 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 21,24 D3S1358 15 D5S818 10,11 D7S820 10,12 D8S1179 12 D13S317 9,11 D16S539 13,14 D18S51 14,16 D19S433 13,15 D21S11 30 FGA 22,23,24 PentaD 9,12 PentaE 8,12 TH01 9,9.3 TPOX 8 vWA 16 D6S1043 11 D12S391 17,24 D2S441 14 -
STR鉴定图
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参考文献
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ABHD5-CPT1B: An Important Way of Regulating Placental Lipid Metabolism in Gestational Diabetes Mellitus (2023-12-01)
作者：Yuqi Yang:1.Department of Medical Genetics, Changzhou maternal and Child Health Care Hospital, Changzhou Medical Center, Nanjing Medical University, No.16 Ding Xiang Road, Changzhou, Jiangsu Province, China. ; Yue Peng:1.Department of Obstetrics, Changzhou maternal and Child Health Care Hospital, Changzhou Medical Center, Nanjing Medical University, No.16 Ding Xiang Road, Changzhou, Jiangsu Province, China. ; Bin Yu:1.Department of Medical Genetics, Changzhou maternal and Child Health Care Hospital, Changzhou Medical Center, Nanjing Medical University, No.16 Ding Xiang Road, Changzhou, Jiangsu Province, China. Electronic address: binyu@njmu.edu.cn. ; Huiyan Wang:1.Department of Obstetrics, Changzhou maternal and Child Health Care Hospital, Changzhou Medical Center, Nanjing Medical University, No.16 Ding Xiang Road, Changzhou, Jiangsu Province, China. ;
期刊：ABHD5-CPT1B: An Important Way of Regulating Placental Lipid Metabolism in Gestational Diabetes Mellitus
DOI：10.1016/j.arcmed.2023.102925
影响因子 ：7.7
引用产品： BeWo 细胞
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BPA induces placental trophoblast proliferation inhibition and fetal growth restriction by inhibiting the expression of SRB1 (2023-04-11)
作者：Yuming Cao:1.Department of Gynaecology and Obstetrics, Zhongnan Hospital of Wuhan University, No 169 of Donghu Road, Wuhan, 430071, Hubei, China. ; 2.Reproductive Medicine Center, Zhongnan Hospital of Wuhan University, Wuhan, China. ; 3.Department of Gynaecology and Obstetrics, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China. ; Sihan Chen:1.School of Basic Medical Sciences, Wuhan University, Wuhan, China. ; Jing Lu:1.Department of Gynaecology and Obstetrics, Zhongnan Hospital of Wuhan University, No 169 of Donghu Road, Wuhan, 430071, Hubei, China. ; Ming Zhang:1.Department of Gynaecology and Obstetrics, Zhongnan Hospital of Wuhan University, No 169 of Donghu Road, Wuhan, 430071, Hubei, China. ; 2.Reproductive Medicine Center, Zhongnan Hospital of Wuhan University, Wuhan, China. ; Lei Shi:1.Reproductive Medicine Center, Zhongnan Hospital of Wuhan University, Wuhan, China. ; Juling Qin:1.Reproductive Medicine Center, Zhongnan Hospital of W
期刊：BPA induces placental trophoblast proliferation inhibition and fetal growth restriction by inhibiting the expression of SRB1
DOI：10.1007/s11356-023-26850-4
影响因子 ：5.8
引用产品： BeWo 细胞
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Friend leukemia integration 1 overexpression decreases endometrial receptivity and induces embryo implantation failure by promoting PART1 transcription in the endometrial epithelial cells (2023-09-26)
作者：Yile Zhang:1.Reproductive Medical Center, The Fifth Clinical Medical College of Shanxi Medical University, Shanxi Provincial People's hospital, Taiyuan, China. ; 2.Reproductive Medical Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. ; Beining Yin:1.Reproductive Medical Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. ; Sichen Li:1.Reproductive Medical Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. ; Yueyue Cui:1.Reproductive Medical Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. ; Jianrong Liu:1.Reproductive Medical Center, The Fifth Clinical Medical College of Shanxi Medical University, Shanxi Provincial People's hospital, Taiyuan, China. ;
期刊：Friend leukemia integration 1 overexpression decreases endometrial receptivity and induces embryo implantation failure by promoting PART1 transcription in the endometrial epithelial cells
影响因子 ：2.7
引用产品： HEC-1-A 细胞 , Ishikawa 细胞 , RL95-2 细胞 , BeWo 细胞
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Biocompatible Assessment of Erythrocyte Membrane-Camouflaged Polymeric PLGA Nanoparticles in Pregnant Mice: Both on Maternal and Fetal/Juvenile Mice (2022-12-01)
作者：Sailing Chen:1.Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China. ; Dongyan Tian:1.Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China. ; Xuewei Yang:1.Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China. ; Qingqing Yin:1.Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China. ; Li Li:1.Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China. ; Yijing Lin:1.Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China. ; Shuangshuang Liu:1.Department of Obstetrics and Gynecology, Th
期刊：Biocompatible Assessment of Erythrocyte Membrane-Camouflaged Polymeric PLGA Nanoparticles in Pregnant Mice: Both on Maternal and Fetal/Juvenile Mice
影响因子 ：7.0
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miR-214-5p suppresses the proliferation, migration and invasion of trophoblast cells in pre-eclampsia by targeting jagged 1 to inhibit notch signaling pathway (2002-00-20)
作者：Fengyan Gong:1.Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun 130021, People's Republic of China. ; Wei Chai:1.Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun 130021, People's Republic of China. ; Junwei Wang:1.Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun 130021, People's Republic of China. ; Huiyan Cheng:1.Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun 130021, People's Republic of China. ; Yuee Shi:1.Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun 130021, People's Republic of China. ; Lifeng Cui:1.Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun 130021, People's Republic of China. Electronic address: Run1019@163.com. ; Guifeng Jia:1.Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun
期刊：miR-214-5p suppresses the proliferation, migration and invasion of trophoblast cells in pre-eclampsia by targeting jagged 1 to inhibit notch signaling pathway
DOI：10.1016/j.acthis.2020.151527
影响因子 ：2.5
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