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产品概述
名称 | CFPAC-1 (人胰腺癌细胞) (STR鉴定正确) |
别称 | CFPac-1; CF PAC-1; CF-PAC1; CF-Pac1; CF Pac1; CFPAC1; CFPac1; CFPAC |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | CFPAC-1细胞是胰腺管腺癌细胞系,建自26岁白人男性囊性纤维变性(CF)的肝转移灶。CFPAC-1细胞表达囊性纤维变性跨膜调节因子(CFTR),CFPAC-1细胞形态为上皮细胞样且顶端微绒毛极化。CFPAC-1细胞表达胰腺管细胞特征性的细胞角蛋白和癌胚抗原,倍增时间为30-32小时。CFPAC-1细胞是亚3倍体的细胞系,36%的细胞的染色体模式数为75。CFPAC-1细胞传代至24代时,可在免疫抑制小鼠中致瘤。 |
年龄(性别) | 男性;26岁 |
组织来源 | 胰腺管腺癌,肝转移 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 胰腺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~30-45 hours |
致瘤性 | Yes, in nude mice (passage 34) |
抗原表达情况 | CA19-9 antigen, 12000 units/mL, epithelial keratins |
基因表达情况 | carcinoembryonic antigen (CEA), 9ng/mL; pancreatic oncofetal antigen (POA), 28ng/mL; adenocarcinoma associated antigen (ACAA), 5000ng/mL; CA 19-9 antigen, 12000 units/mL; epithelial keratins |
保藏机构 | ATCC; CRL-1918 ECACC; 91112501 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 10 D2S1338 18,23 D3S1358 16 D5S818 10,11 D7S820 8,10 D8S1179 11,15 D13S317 12 D16S539 9,11 D18S51 12 D19S433 13,15 D21S11 30,31.2 FGA 21,22 PentaD 11,13 PentaE 10,12 TH01 8 TPOX 8 vWA 17 D6S1043 20 D12S391 17 D2S441 10,14 -
STR鉴定图
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参考文献
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Concurrent inhibition of pBADS99 synergistically improves MEK inhibitor efficacy in KRASG12D-mutant pancreatic ductal adenocarcinoma (2024-02-26)
期刊:Cell Death & Disease
DOI:10.1038/s41419-024-06551-7
影响因子 :9.0
引用产品: SW 1990 细胞 , PANC-1 细胞 , AsPC-1 细胞 , CFPAC-1 细胞
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METTL3 promotes pancreatic cancer proliferation and stemness by increasing stability of ID2 mRNA in a m6A-dependent manner (2023-05-19)
期刊:CANCER LETTERS
DOI:10.1016/j.canlet.2023.216222
影响因子 :9.7
引用产品: PC 细胞 , CFPAC-1 细胞 , HPDEC 细胞 , Panc02 细胞
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High glucose-upregulated PD-L1 expression through RAS signaling-driven downregulation of PTRH1 leads to suppression of T cell cytotoxic function in tumor environment (2023-07-11)
期刊:Journal of Translational Medicine
DOI:10.1186/s12967-023-04302-4
影响因子 :7.4
引用产品: CFPAC-1 细胞 , Panc02 细胞
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Construction and comprehensive analysis of a novel prognostic signature associated with pyroptosis molecular subtypes in patients with pancreatic adenocarcinoma (2023-02-03)
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2023.1111494
影响因子 :7.3
引用产品: CFPAC-1 细胞 , CFPAC-1细胞专用培养基
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Crosstalk of ferroptosis regulators and tumor immunity in pancreatic adenocarcinoma: novel perspective to mRNA vaccines and personalized immunotherapy (2023-06-27)
期刊:APOPTOSIS
DOI:10.1007/s10495-023-01868-8
影响因子 :7.2
引用产品: HPNE 细胞 , CFPAC-1 细胞 , SW 1990 细胞 , BxPC-3 细胞
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PSMD12 interacts with CDKN3 and facilitates pancreatic cancer progression (2023-04-10)
期刊:CANCER GENE THERAPY
DOI:10.1038/s41417-023-00609-y
影响因子 :6.4
引用产品: CFPAC-1 细胞 , IMDM 培养基 , Leibovitz's L-15 培养基
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5′-tRF-19-Q1Q89PJZ Suppresses the Proliferation and Metastasis of Pancreatic Cancer Cells via Regulating Hexokinase 1-Mediated Glycolysis (2023-10-12)
期刊:Biomolecules
影响因子 :5.5
引用产品: MIA PaCa-2 细胞 , PANC-1 细胞 , CFPAC-1 细胞 , Capan-2 细胞 , SW 1990 细胞 , BxPC-3 细胞 , AsPC-1 细胞 , 人胰腺上皮细胞
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RBM15 Promates the Proliferation, Migration and Invasion of Pancreatic Cancer Cell Lines (2023-02-08)
期刊:Cancers
影响因子 :5.2
引用产品: HPNE 细胞 , CFPAC-1 细胞 , BxPC-3 细胞
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COL12A1 Acts as a Novel Prognosis Biomarker and Activates Cancer-Associated Fibroblasts in Pancreatic Cancer through Bioinformatics and Experimental Validation (2023-02-26)
期刊:Cancers
影响因子 :5.2
引用产品: PANC-1 细胞 , BxPC-3 细胞 , CFPAC-1 细胞 , PATU8988 细胞 , AsPC-1 细胞 , MIA PaCa-2 细胞
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Identifying endoplasmic reticulum stress-related molecular subtypes and prognostic model for predicting the immune landscape and therapy response in pancreatic cancer (2023-10-09)
期刊:Aging-US
影响因子 :5.2
引用产品: CFPAC-1 细胞 , PANC-1 细胞
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GID2 Interacts With CDKN3 and Regulates Pancreatic Cancer Growth and Apoptosis (2023-02-23)
期刊:LABORATORY INVESTIGATION
DOI:10.1016/j.labinv.2023.100122
影响因子 :5.0
引用产品: CFPAC-1 细胞 , IMDM 培养基 , Leibovitz's L-15 培养基
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Identification of a chemoresistance-related prognostic gene signature by comprehensive analysis and experimental validation in pancreatic cancer (2023-05-12)
期刊:Frontiers in Oncology
影响因子 :4.7
引用产品: AsPC-1 细胞 , CFPAC-1 细胞 , PANC-1 细胞 , IMDM 培养基 , DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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PANoptosis-related molecular subtype and prognostic model associated with the immune microenvironment and individualized therapy in pancreatic cancer (2023-07-14)
期刊:Frontiers in Oncology
影响因子 :4.7
引用产品: PANC-1 细胞 , CFPAC-1 细胞
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Identification and characterization of interferon-γ signaling-based personalized heterogeneity and therapeutic strategies in patients with pancreatic cancer (2023-10-24)
期刊:Frontiers in Oncology
影响因子 :4.7
引用产品: BxPC-3 细胞 , PANC-1 细胞 , CFPAC-1 细胞 , MIA PaCa-2 细胞
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Involvement of Ataxin-3 (ATXN3) in the malignant progression of pancreatic cancer via deubiquitinating HDAC6 (2023-07-07)
期刊:PANCREATOLOGY
影响因子 :3.6
引用产品: CFPAC-1 细胞
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A prospective prognostic signature for pancreatic adenocarcinoma based on ubiquitination-related mRNA-lncRNA with experimental validation in vitro and vivo (2023-08-04)
期刊:FUNCTIONAL & INTEGRATIVE GENOMICS
DOI:10.1007/s10142-023-01158-1
影响因子 :2.9
引用产品: CFPAC-1 细胞 , PANC-1 细胞 , IMEM
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System analysis based on the pyroptosis-related genes identifies GSDMC as a novel therapy target for pancreatic adenocarcinoma (2022-10-05)
期刊:Journal of Translational Medicine
DOI:10.1186/s12967-022-03632-z
影响因子 :8.4
引用产品: CFPAC-1 细胞 , PANC-1 细胞
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Synthesis and anti-tumor activity of nitrogen-containing derivatives of the natural product diphyllin (2022-08-26)
期刊:EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
DOI:10.1016/j.ejmech.2022.114708
影响因子 :7.1
引用产品: MIA PaCa-2 细胞 , CFPAC-1 细胞 , PaTu 8988t 细胞
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Upstream stimulatory factor 2 inhibits erastin-induced ferroptosis in pancreatic cancer through transcriptional regulation of pyruvate kinase M2 (2022-09-20)
期刊:BIOCHEMICAL PHARMACOLOGY
影响因子 :6.1
引用产品: PANC-1 细胞 , MIA PaCa-2 细胞 , BxPC-3 细胞 , AsPC-1 细胞 , CFPAC-1 细胞 , SW 1990 细胞 , CFPAC-1细胞专用培养基 , SW 1990细胞专用培养基 (L15)
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miR-23a-5p inhibits cell proliferation and invasion in pancreatic ductal adenocarcinoma by suppressing ECM1 expression (2005-09-20)
期刊:American Journal of Translational Research
DOI:PMID:31217868
影响因子 :2.2
引用产品: AsPC-1 细胞 , CFPAC-1 细胞
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