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产品概述
名称 | Ishikawa (人子宫内膜癌细胞) (STR鉴定正确) |
别称 | ISHIKAWA; ISHI |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
年龄(性别) | 女性;39岁 |
组织来源 | 子宫 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 子宫内膜腺癌 |
生物安全等级 | BSL-1 |
倍增时间 | ~32-40 hours |
保藏机构 | ECACC; 99040201 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12,13 D2S1338 19,21 D3S1358 16 D5S818 10,11 D7S820 9,10 D8S1179 13,16 D13S317 9,12 D16S539 9 D18S51 12,21 D19S433 12.2,14 D21S11 28 FGA 20,21,22,23 PentaD 10,11 PentaE 11,19 TH01 9,10 TPOX 8 vWA 14,17 D6S1043 12,18 D12S391 18,20,21 D2S441 12 -
STR鉴定图
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参考文献
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Integrating network pharmacology to investigate the mechanism of quercetin's action through AKT inhibition in co-expressed genes associated with polycystic ovary syndrome and endometrial cancer (2025-01-05)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2025.139468
影响因子 :7.7
引用产品: Ishikawa 细胞 , RL95-2 细胞
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Identification and validation of the important role of KIF11 in the development and progression of endometrial cancer (2025-01-13)
期刊:Journal of Translational Medicine
DOI:10.1186/s12967-025-06081-6
影响因子 :6.1
引用产品: HEC-1-B 细胞 , Ishikawa 细胞
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NCAPG promotes the malignant progression of endometrioid cancer through LEF1/SEMA7A/PI3K-AKT (2025-01-01)
期刊:Journal of Cancer
影响因子 :3.3
引用产品: HEC-1-A 细胞 , HEC-1-B 细胞 , Ishikawa 细胞
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IGF2BP3 is upregulated in endometrial cancer and tightly regulates the growth of drug-resistant endometrial cancer cells via HMGA1 (2025-01-29)
期刊:SCIENCE PROGRESS
影响因子 :2.6
引用产品: Ishikawa 细胞 , AN3 CA 细胞
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High FGF18 expression levels predict poor prognosis in endometrial carcinoma patients and promote tumor growth and metastasis (2025-01-24)
期刊:JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
影响因子 :1.4
引用产品: Ishikawa 细胞
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Proteogenomic insights into early-onset endometrioid endometrial carcinoma: predictors for fertility-sparing therapy response (2024-04-02)
期刊:NATURE GENETICS
DOI:10.1038/s41588-024-01703-z
影响因子 :30.8
引用产品: Ishikawa 细胞
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Sesamolin serves as an MYH14 inhibitor to sensitize endometrial cancer to chemotherapy and endocrine therapy via suppressing MYH9/GSK3β/β-catenin signaling (2024-05-02)
期刊:CELLULAR & MOLECULAR BIOLOGY LETTERS
DOI:10.1186/s11658-024-00583-9
影响因子 :9.2
引用产品: Ishikawa 细胞 , KLE 细胞
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Phosphocholine inhibits proliferation and reduces stemness of endometrial cancer cells by downregulating mTOR-c-Myc signaling (2024-12-16)
期刊:CELLULAR AND MOLECULAR LIFE SCIENCES
DOI:10.1007/s00018-024-05517-4
影响因子 :6.2
引用产品: AN3 CA 细胞 , Ishikawa 细胞
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Dioscin from Polygonatum sibiricum induces apoptosis and autophagy in Ishikawa human endometrial cancer cell and in vivo (2024-10-23)
期刊:Food Science and Human Wellness
DOI:10.26599/FSHW.2022.9250209
影响因子 :5.6
引用产品: Ishikawa 细胞 , 293 [HEK-293] 细胞
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ERP29 regulates the proliferation of endometrial carcinoma via M6A modification (2024-08-12)
期刊:LIFE SCIENCES
影响因子 :5.2
引用产品: HEC-1-A 细胞 , Ishikawa 细胞 , DMEM高糖 培养基
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NSUN2-mediated m5C methylation impairs endometrial receptivity (2024-01-17)
期刊:LABORATORY INVESTIGATION
DOI:10.1016/j.labinv.2024.100327
影响因子 :5.1
引用产品: Ishikawa 细胞 , BeWo 细胞
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HMGB1-mediated transcriptional activation of circadian gene TIMELESS contributes to endometrial cancer progression through Wnt-β-catenin pathway (2024-01-10)
期刊:CELLULAR SIGNALLING
DOI:10.1016/j.cellsig.2024.111045
影响因子 :4.8
引用产品: Ishikawa 细胞 , KLE 细胞 , Ishikawa细胞专用培养基 , KLE细胞专用培养基
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Oxidative stress drives endometrial fibrosis via TGF-β1/MAPK signaling pathway in breast cancer (2024-11-16)
期刊:FASEB JOURNAL
影响因子 :4.4
引用产品: Ishikawa 细胞 , MDA-MB-231 细胞
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Long noncoding RNA VPS9D1-AS1 promotes the progression of endometrial cancer via regulation of the miR-187-3p/S100A4 axis (2024-07-02)
期刊:ENVIRONMENTAL TOXICOLOGY
影响因子 :4.4
引用产品: 人子宫内膜上皮细胞 , HEC-1-A 细胞 , KLE 细胞 , Ishikawa 细胞 , RL95-2 细胞
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SIM2: Its Prognostic Significance and Oncogenic Role in Endometrial Carcinoma (2024-01-26)
期刊:OncoTargets and Therapy
影响因子 :4.0
引用产品: Ishikawa 细胞 , HEC-1-A 细胞
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WEE1 Inhibitors Mediate Antitumor Effects on Endometrial Cancer through Activation of Innate Immune Responses (2024-01-01)
期刊:Journal of Cancer
影响因子 :3.9
引用产品: Ishikawa 细胞
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Repurposing propofol for breast cancer therapy through promoting apoptosis and arresting cell cycle (2024-09-27)
期刊:ONCOLOGY REPORTS
影响因子 :3.8
引用产品: MKN-45 细胞 , Ishikawa 细胞 , 特级胎牛血清
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Lactamase β reprograms lipid metabolism to inhibit the progression of endometrial cancer through attenuating MDM2-mediated p53 ubiquitination and degradation (2024-12-31)
期刊:ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
影响因子 :3.8
引用产品: RL95-2 细胞 , HEC-1-A 细胞 , Ishikawa 细胞 , AN3 CA 细胞 , KLE 细胞
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Expression Analysis of FANCD2 in Endometrial Carcinoma (2024-12-05)
期刊:Cancer Management and Research
影响因子 :2.5
引用产品: Ishikawa 细胞
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Polyphyllin VII enhances the sensitivity of endometrial carcinoma cells to medroxyprogesterone acetate through upregulating miR?33a?5p expression (2024-11-22)
期刊:Oncology Letters
影响因子 :2.5
引用产品: Ishikawa 细胞
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Participation of Long Noncoding RNA FOXP4-AS1 in the Development and Progression of Endometrioid Carcinoma with Epigenetically Silencing DUSP5 (2024-03-20)
期刊:CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
影响因子 :2.4
引用产品: KLE 细胞 , RL95-2 细胞 , Ishikawa 细胞
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SOX4 inhibits ferroptosis and promotes proliferation of endometrial cancer cells via the p53/SLC7A11 signaling (2024-09-24)
期刊:JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
影响因子 :1.6
引用产品: HEC-1-A 细胞 , RL95-2 细胞 , AN3 CA 细胞 , Ishikawa 细胞
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Circular single-stranded DNA as switchable vector for gene expression in mammalian cells (2023-10-20)
期刊:Nature Communications
DOI:10.1038/s41467-023-42437-6
影响因子 :16.6
引用产品: B16 细胞 , MCF7 [MCF-7] 细胞 , AC16 细胞 , U-87 MG 细胞 , Hela 细胞 , HELF 细胞 , 293T [HEK-293T] 细胞 , Hepa 1-6 细胞 , NCTC clone 929 [L cell, L-929] 细胞 , A549 [A-549] 细胞 , 5637 细胞 , Ishikawa 细胞 , LoVo 细胞
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TMT-based quantitative proteomic analysis of spheroid cells of endometrial cancer possessing cancer stem cell properties (2023-05-04)
期刊:Stem Cell Research & Therapy
DOI:10.1186/s13287-023-03348-x
影响因子 :7.5
引用产品: Ishikawa 细胞
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Construction of the novel immune risk scoring system related to CD8+ T cells in uterine corpus endometrial carcinoma (2023-06-22)
期刊:Cancer Cell International
DOI:10.1186/s12935-023-02966-y
影响因子 :5.8
引用产品: HEC-1-A 细胞 , Ishikawa 细胞
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hsa_circ_0005991 promotes epithelial-mesenchymal transition by regulating miR-30b-3p/Cdc42EP1 axis in ovary endometriosis (2023-09-25)
期刊:GENOMICS
DOI:10.1016/j.ygeno.2023.110718
影响因子 :4.4
引用产品: Ishikawa 细胞 , 293T [HEK-293T] 细胞
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Therapeutic potential of miRNAs in placental extracellular vesicles in ovarian and endometrial cancer (2023-10-06)
期刊:Human Cell
DOI:10.1007/s13577-023-00986-4
影响因子 :4.3
引用产品: SK-OV-3 细胞 , Ishikawa 细胞
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Asparanin A exerts cytotoxicity on human endometrial cancer Ishikawa cells via regulating miR-6236-p5_4 expression (2023-06-25)
期刊:FOOD AND CHEMICAL TOXICOLOGY
影响因子 :4.3
引用产品: KLE 细胞
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Inhibition of ATM with KU-55933 Sensitizes Endometrial Cancer Cell Lines to Olaparib (2023-12-31)
期刊:OncoTargets and Therapy
影响因子 :4.0
引用产品: Ishikawa 细胞 , HEC-1-B 细胞 , AN3 CA 细胞 , KLE 细胞 , RL95-2 细胞
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YBX2 modulates mRNA stability via interaction with YTHDF2 in endometrial cancer cells (2023-04-06)
期刊:EXPERIMENTAL CELL RESEARCH
DOI:10.1016/j.yexcr.2023.113586
影响因子 :3.7
引用产品: Ishikawa 细胞 , 293 [HEK-293] 细胞 , Hela 细胞 , DMEM高糖 培养基
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Ferroptosis-related lncRNA model based on CFAP58-DT for predicting prognosis and immunocytes infiltration in endometrial cancer (2023-02-10)
期刊:Annals of Translational Medicine
影响因子 :3.6
引用产品: HEC-1-A 细胞 , Ishikawa 细胞 , 特级胎牛血清 , 增强型细胞活力检测试剂盒 (CCK-8)
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Explore the anti-inflammation mechanism of safflower total flavonoids in the treatment of endometritis based on celluar transcriptomics (2023-09-20)
期刊:JOURNAL OF PHARMACY AND PHARMACOLOGY
影响因子 :3.3
引用产品: 特级胎牛血清 , Ishikawa 细胞
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The lncRNA LINC00339-encoded peptide promotes trophoblast adhesion to endometrial cells via MAPK and PI3K-Akt signaling pathways (2023-12-05)
期刊:JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
DOI:10.1007/s10815-023-02995-6
影响因子 :3.1
引用产品: Ishikawa 细胞 , JAR 细胞
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ZAP70 interaction with 13 mRNAs as a potential immunotherapeutic target for endometrial cancer (2023-04-06)
期刊:Oncology Letters
影响因子 :2.9
引用产品: HEC-1-A 细胞 , Ishikawa 细胞 , 特级胎牛血清
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IGFBP?rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway (2023-03-03)
期刊:Experimental and Therapeutic Medicine
影响因子 :2.7
引用产品: Ishikawa 细胞 , HEC-1-A 细胞 , RL95-2 细胞 , HEC-1-B 细胞 , AN3 CA 细胞 , THP-1 细胞
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Friend leukemia integration 1 overexpression decreases endometrial receptivity and induces embryo implantation failure by promoting PART1 transcription in the endometrial epithelial cells (2023-09-26)
期刊:PeerJ
影响因子 :2.7
引用产品: HEC-1-A 细胞 , Ishikawa 细胞 , RL95-2 细胞 , BeWo 细胞
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LINC01857 Exacerbates the Malignant Behaviors of Endometrial Carcinoma Cells by Sponging miR-19b-3p and Recruiting ELAVL1 to Upregulate MYCN (2023-03-28)
期刊:GYNECOLOGIC AND OBSTETRIC INVESTIGATION
影响因子 :2.1
引用产品: HEC-1-A 细胞 , HEC-1-B 细胞 , Ishikawa 细胞 , RL95-2 细胞
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Talin1 regulates glucose metabolism and endometrial receptivity via GLUT-4 in patients with polycystic ovary syndrome and insulin resistance (2023-07-03)
期刊:GYNECOLOGICAL ENDOCRINOLOGY
DOI:10.1080/09513590.2023.2231085
影响因子 :2.0
引用产品: Ishikawa 细胞
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miR‐548ag functions as an oncogene by suppressing MOB1B in the development of obesity‐related endometrial cancer (2022-12-23)
期刊:CANCER SCIENCE
影响因子 :6.5
引用产品: Ishikawa 细胞 , DMEM高糖 培养基 , 青霉素-链霉素溶液(双抗),100× , 293T [HEK-293T] 细胞 , 特级胎牛血清
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Integrated miRNA and mRNA omics reveal dioscin suppresses migration and invasion via MEK/ERK and JNK signaling pathways in human endometrial carcinoma in vivo and in vitro (2022-12-09)
期刊:JOURNAL OF ETHNOPHARMACOLOGY
影响因子 :5.2
引用产品: Ishikawa 细胞 , 293 [HEK-293] 细胞
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The potential role of methyltransferase-like 5 in deficient mismatch repair of uterine corpus endometrial carcinoma (2022-02-15)
期刊:Bioengineered
DOI:10.1080/21655979.2022.2036912
影响因子 :4.9
引用产品: 人子宫内膜上皮细胞 , KLE 细胞 , RL95-2 细胞 , Ishikawa 细胞
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Circ_0075960 targets the miR-202-5p/CTNND1 axis to promote the growth and migration of endometrial carcinoma cells via regulating Wnt/β-catenin signaling activity (2022-11-14)
期刊:Journal of Gynecologic Oncology
影响因子 :4.8
引用产品: HEC-1-B 细胞 , 特级胎牛血清 , 人子宫内膜基质细胞 , DMEM高糖 培养基 , Ishikawa 细胞 , RL95-2 细胞
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FANCD2 promotes the malignant behavior of endometrial cancer cells and its prognostic value (2022-10-15)
期刊:EXPERIMENTAL CELL RESEARCH
DOI:10.1016/j.yexcr.2022.113388
影响因子 :4.2
引用产品: HEC-1-B 细胞 , Ishikawa 细胞
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Diane-35 and Metformin Induce Autophagy and Apoptosis in Polycystic Ovary Syndrome Women with Early-Stage Endometrial Carcinoma (2022-01-12)
期刊:Genes
影响因子 :3.5
引用产品: Ishikawa 细胞
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GLP1R inhibits the progression of endometrial carcinoma through activation of cAMP/PKA pathway (2022-08-21)
期刊:JOURNAL OF CLINICAL LABORATORY ANALYSIS
影响因子 :3.1
引用产品: 人子宫内膜上皮细胞 , Ishikawa 细胞 , RL95-2 细胞 , 人子宫内膜上皮细胞完全培养基 , DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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T-Box Transcription Factor 2 Enhances Chemoresistance of Endometrial Cancer by Mediating NRF2 Expression (2022-08-01)
期刊:CURRENT PROTEIN & PEPTIDE SCIENCE
DOI:10.2174/1389203723666220823152239
影响因子 :3.1
引用产品: Ishikawa 细胞 , 293T [HEK-293T] 细胞
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Circ_0039569 contributes to the paclitaxel resistance of endometrial cancer via targeting miR-1271-5p/PHF6 pathway (2022-08-12)
期刊:ANTI-CANCER DRUGS
DOI:10.1097/CAD.0000000000001377
影响因子 :2.4
引用产品: Ishikawa 细胞 , RL95-2 细胞 , 人子宫内膜基质细胞
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Growth hormone inhibits the JAK/STAT3 pathway by regulating SOCS1 in endometrial cells in vitro: A clue to enhance endometrial receptivity in recurrent implantation failure (2022-12-22)
期刊:EUROPEAN JOURNAL OF HISTOCHEMISTRY
影响因子 :2.0
引用产品: Ishikawa 细胞 , HEC-1-A 细胞
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A short peptide encoded by long non-coding RNA small nucleolar RNA host gene 6 promotes cell migration and epithelial–mesenchymal transition by activating transforming growth factor-beta/SMAD signaling pathway in human endometrial cells (2022-11-17)
期刊:JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
影响因子 :1.7
引用产品: Ishikawa 细胞
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In situ delivery of apoptotic bodies derived from mesenchymal stem cells via a hyaluronic acid hydrogel: A therapy for intrauterine adhesions (2021-10-22)
期刊:Bioactive Materials
DOI:10.1016/j.bioactmat.2021.10.025
影响因子 :18.9
引用产品: Ishikawa 细胞 , RL95-2 细胞 , PUMC-HUVEC-T1
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Exosomal transfer of tumor-associated macrophage-derived hsa_circ_0001610 reduces radiosensitivity in endometrial cancer (2021-08-30)
期刊:Cell Death & Disease
DOI:10.1038/s41419-021-04087-8
影响因子 :9.0
引用产品: THP-1 细胞 , Ishikawa 细胞 , HEC-1-B 细胞
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Talin1 regulates endometrial adhesive capacity through the Ras signaling pathway (2021-03-09)
期刊:Life Sciences
影响因子 :6.8
引用产品: 小鼠子宫内膜上皮细胞完全培养基 , Ishikawa 细胞 , JEG-3 细胞 , MEM (含NEAA) 培养基
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N-methyladenosine reader YTHDF2-mediated long noncoding RNA FENDRR degradation promotes cell proliferation in endometrioid endometrial carcinoma (2021-03-10)
期刊:Laboratory Investigation
DOI:10.1038/s41374-021-00543-3
影响因子 :5.5
引用产品: Ishikawa 细胞 , HEC-1-B 细胞
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Asparanin A inhibits cell migration and invasion in human endometrial cancer via Ras/ERK/MAPK pathway (2021-02-06)
期刊:Food And Chemical Toxicology
影响因子 :4.3
引用产品: Ishikawa 细胞
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hsa_circ_0001610 knockdown modulates miR-646-STAT3 axis to suppress endometrial carcinoma progression (2021-04-20)
期刊:Journal Of Gene Medicine
DOI:10.1002/jgm.3337
影响因子 :3.5
引用产品: HEC-1-B 细胞 , KLE 细胞 , Ishikawa 细胞
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6-Shogaol mediated ROS production and apoptosis via endoplasmic reticulum and mitochondrial pathways in human endometrial carcinoma Ishikawa cells (2020-09-09)
期刊:Journal of Functional Foods
影响因子 :5.6
引用产品: Ishikawa 细胞
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Plumbagin induces Ishikawa cell cycle arrest, autophagy, and apoptosis via the PI3K/Akt signaling pathway in endometrial cancer (2020-12-28)
期刊:Food And Chemical Toxicology
影响因子 :4.3
引用产品: Ishikawa 细胞
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Exosomal-lncRNA DLEU1 Accelerates the Proliferation, Migration, and Invasion of Endometrial Carcinoma Cells by Regulating microRNA-E2F3 (2020-08-25)
期刊:OncoTargets and Therapy
影响因子 :4.0
引用产品: KLE 细胞 , Ishikawa 细胞 , HEC-1-A 细胞 , RL95-2 细胞
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Silencing of Long Non-Coding RNA LINC01106 Suppresses the Proliferation, Migration and Invasion of Endometrial Cancer Cells Through Regulating the miR-449a/MET Axis (2020-09-29)
期刊:OncoTargets and Therapy
影响因子 :4.0
引用产品: KLE 细胞 , Ishikawa 细胞 , HEC-1-A 细胞
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Knockdown of Trop2 inhibits proliferation and migration and induces apoptosis of endometrial cancer cells via AKT/β‐catenin pathway (2020-01-22)
期刊:Cell Biochemistry and Function
DOI:10.1002/cbf.3450
影响因子 :3.6
引用产品: Ishikawa 细胞 , KLE 细胞 , 人子宫内膜上皮细胞
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Asparanin A from Asparagus officinalis L. Induces G0/G1 Cell Cycle Arrest and Apoptosis in Human Endometrial Carcinoma Ishikawa Cells via Mitochondrial and PI3K/AKT Signaling Pathways (2019-12-27)
期刊:Journal Of Agricultural And Food Chemistry
影响因子 :6.1
引用产品: Ishikawa 细胞 , 293 [HEK-293] 细胞
FAQs
Q:RL95-2(人子宫内膜癌细胞)和Ishikawa(人子宫内膜癌细胞)这两种细胞有什么不同?
A:二者详细资料及区别如下: Cellosaurus cell line Ishikawa (CVCL_2529) https://www.cellosaurus.org/CVCL_2529 Cellosaurus cell line RL95-2 (CVCL_0505) https://www.cellosaurus.org/CVCL_0505
Q:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
A:部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。
Q:冻存细胞运输与常温细胞运输相比有什么区别?
A:发货有两种形式,常温或者冻存都可以的,常温就是货期一周左右(具体以购买当时销售报的货期为准),我们培养好一个T25瓶发货;冻存细胞如果有库存下单第二天能发货,细胞系冻存细胞担心客户复苏不成功所以赠送了一管,实际发货2管;冻存细胞发货是10公斤干冰及顺丰运输,所以冻存细胞需额外支付干冰及运输费。
Q:培养细胞的培养瓶可以重复利用吗?
A:一般不建议重复利用,特别是贴壁不牢固细胞,重复利用会导致吸附性变差,漂浮增多;一个T25瓶建议使用最多不超过3次,其次需要注意无菌操作,避免污染。
Q:冻存的细胞出现颜色不一致,有的红有的粉,对复苏会有影响吗?
A:这种情况容易在不同冻存批次间出现,与冻存细胞的密度、冻存液配方、温度导致pH变化等有关,偶尔有遇到这种情况,不是绝对性对细胞状态有影响,可以复苏看下。
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