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产品概述
名称 | WI-38 (人胚肺细胞) (STR鉴定正确) |
别称 | Wi-38; WI 38; WI38; Wistar Institute-38; AG06814-J; AG06814-M; AG06814-N |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞为有限传代细胞系,传代次数过高会导致细胞生长特性发生变化,实验室测试可传4-5代,建议到货3代内完成实验。 |
背景描述 | WI-38人二倍体细胞是来自妊娠3个月的正常胚胎肺组织。WI-38细胞是首株用于人类疫苗制备的人二倍体细胞系,培养液中添加TNF-α可以促进WI-38细胞生长。 |
年龄(性别) | 女;3个月妊娠期胎儿 |
组织来源 | 正常肺 |
细胞类型 | 有限细胞系 |
生物安全等级 | BSL-1 |
保藏机构 | ATCC; CCL-75 ATCC; CRL-7728ECACC; 90020107 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10,12 D2S1338 19,25 D3S1358 16,17 D5S818 10 D7S820 9,11 D8S1179 14 D13S317 11 D16S539 11,12 D18S51 16,18 D19S433 13,16.2 D21S11 30,30.2 FGA 22,24 PentaD 13 PentaE 13,14 TH01 8,9.3 TPOX 8 vWA 19,20 D6S1043 11,19 D12S391 21,23 D2S441 11 -
STR鉴定图
参考文献
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Assessment of Mononuclear/Dinuclear copper acylhydrazone complexes for lung cancer treatment (2024-01-14)
作者:Youru Wu:1.The Laboratory of Respiratory Disease, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, China. ; Daqi Wu:1.School of Pharmacy, Guilin Medical University, Guilin, Guangxi, China. ; Jianfeng Lan:1.Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair, the Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, China. ; Aili Li:1.The Laboratory of Respiratory Disease, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, China. ; Lixia Hou:1.The Laboratory of Respiratory Disease, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, China. ; Yourui Xu:1.School of Pharmacy, Guilin Medical University, Guilin, Guangxi, China. Electronic address: xuyourui1980@sina.com. ; Yi Gou:1.The Laboratory of Respiratory Disease, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi, China. Electronic address: sanchuanok@126.com. ;
期刊:Assessment of Mononuclear/Dinuclear copper acylhydrazone complexes for lung cancer treatment
DOI:10.1016/j.bioorg.2024.107122
影响因子 :5.1
引用产品: A549 [A-549] 细胞 , A549/DDP 细胞 , WI-38 细胞
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Design, synthesis and biological evaluation of rhein–piperazine–furanone hybrids as potential anticancer agents (2024-02-13)
作者:Yu He:1.State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering, Ningxia Research Center for Natural Medicine Engineering and Technology, College of Chemistry and Chemical Engineering, Ningxia University 489 Helanshan West Road Yinchuan 750021 China weimengxue@nxu.edu.cn. ; Si-Si Zhang:1.State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering, Ningxia Research Center for Natural Medicine Engineering and Technology, College of Chemistry and Chemical Engineering, Ningxia University 489 Helanshan West Road Yinchuan 750021 China weimengxue@nxu.edu.cn. ; Meng-Xue Wei:1.State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering, Ningxia Research Center for Natural Medicine Engineering and Technology, College of Chemistry and Chemical Engineering, Ningxia University 489 Helanshan West Road Yinchuan 750021 China weimengxue@nxu.edu.cn. ;
期刊:Design, synthesis and biological evaluation of rhein–piperazine–furanone hybrids as potential anticancer agents
影响因子 :4.1
引用产品: A549 [A-549] 细胞 , NCI-H460 [H460] 细胞 , PC-9 细胞 , Calu-1 细胞 , SK-BR-3 [SKBR3] 细胞 , SK-OV-3 细胞 , 786-O [786-0] 细胞 , HuH-7 细胞 , HCT 116 细胞 , Hela 细胞 , WI-38 细胞 , MCF 10A 细胞 , KGN 细胞 , 293T [HEK-293T] 细胞 , DMEM高糖 培养基 , MEM 培养基 , DMEM/F12 培养基 , McCoy's 5A 培养基 , RPMI-1640 培养基 , 0.25%胰蛋白酶溶液 (含EDTA,溶于PBS)
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Selective degradation of BRD4 suppresses lung cancer cell proliferation using GSH-responsive PROTAC precursors (2023-09-03)
作者:Heli Fan:1.Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin Medical University, Tianjin 300070, PR China. ; Zhili Zhou:1.Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, PR China. ; Dehao Yu:1.Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin Medical University, Tianjin 300070, PR China. ; Jing Sun:1.Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin Medical University, Tianjin 300070, PR
期刊:Selective degradation of BRD4 suppresses lung cancer cell proliferation using GSH-responsive PROTAC precursors
DOI:10.1016/j.bioorg.2023.106793
影响因子 :5.1
引用产品: WI-38 细胞 , HULEC-5a 细胞 , NCI-H1299 细胞 , A549 [A-549] 细胞
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Hydrogen Peroxide-Inducible PROTACs for Targeted Protein Degradation in Cancer Cells (2023-07-18)
作者:Dehao Yu:1.Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, The Province and Ministry Co-sponsored Collaborative Innovation Center, for Medical Epigenetics, Tianjin Medical University, Tianjin, 300070, China. ; Heli Fan:1.Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, The Province and Ministry Co-sponsored Collaborative Innovation Center, for Medical Epigenetics, Tianjin Medical University, Tianjin, 300070, China. ; Zhili Zhou:1.Key Laboratory of Immune Microenvironment and Disease, Ministry of Education), Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, China. ; Ying Zhang:1.Key Laboratory of Immune Microenvironment and Disease, Ministry of Education), Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, China. ; Jing Sun:1.Tianj
期刊:Hydrogen Peroxide-Inducible PROTACs for Targeted Protein Degradation in Cancer Cells
影响因子 :3.2
引用产品: A549 [A-549] 细胞 , NCI-H1299 细胞 , NCI-H1975 细胞 , NCI-H520 细胞 , WI-38 细胞 , HULEC-5a 细胞
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CIRC_0012535 CONTRIBUTES TO LIPOPOLYSACCHARIDE-INDUCED FETAL LUNG FIBROBLAST APOPTOSIS AND INFLAMMATION TO REGULATE INFANTILE PNEUMONIA DEVELOPMENT BY MODULATING THE MIR-338-3P/IL6R SIGNALING (2023-05-22)
作者:Xing Fang:1.Department of PICU, Huizhou Central People's Hospital, Huizhou, Guangdong, China. ; Wenjing Mei: Rihua Zeng: Li Zou: Xuefei Zeng: Shanghong Tang:
期刊:CIRC_0012535 CONTRIBUTES TO LIPOPOLYSACCHARIDE-INDUCED FETAL LUNG FIBROBLAST APOPTOSIS AND INFLAMMATION TO REGULATE INFANTILE PNEUMONIA DEVELOPMENT BY MODULATING THE MIR-338-3P/IL6R SIGNALING
DOI:10.1097/SHK.0000000000002111
影响因子 :3.1
引用产品: WI-38 细胞
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FOXA2 attenuates lipopolysaccharide‑induced pneumonia by inhibiting the inflammatory response, oxidative stress and apoptosis through blocking of p38/STAT3 signaling (2023-08-17)
作者:Zhibin Xue:1.Department of Pediatrics, The Affiliated Hospital of Putian University, Putian, Fujian 351100, P.R. China. ; Yinglin Li:1.Department of Pediatrics, The Affiliated Hospital of Putian University, Putian, Fujian 351100, P.R. China. ; Shiji Xiao:1.Department of Pediatrics, The Affiliated Hospital of Putian University, Putian, Fujian 351100, P.R. China. ; Hanqing Zhang:1.Department of Pediatrics, The Affiliated Hospital of Putian University, Putian, Fujian 351100, P.R. China. ; Jianzhang Xu:1.Department of Pediatrics, Putian Children's Hospital, Putian, Fujian 351100, P.R. China. ;
期刊:FOXA2 attenuates lipopolysaccharide‑induced pneumonia by inhibiting the inflammatory response, oxidative stress and apoptosis through blocking of p38/STAT3 signaling
影响因子 :2.7
引用产品: WI-38 细胞 , MEM (含NEAA) 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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Downregulation of circ_0035292 Alleviates LPS-Induced WI-38 Cell Injury via Targeting miR-494-3p/TLR4 Pathway in Infantile Pneumonia (2023-07-27)
作者:Zhenzhao Dai:1.Department of Pediatrics, Affiliated Hospital of Jinggangshan University, Ji'an, Jiangxi, China. ; Jiansheng Hu:1.Jinggangshan University, 28 Xueyuan Road, Qingyuan District, Ji'an, 343000, Jiangxi, China. ; Zhiying Luo:1.Jinggangshan University, 28 Xueyuan Road, Qingyuan District, Ji'an, 343000, Jiangxi, China. ; Jianhua Xiao:1.Jinggangshan University, 28 Xueyuan Road, Qingyuan District, Ji'an, 343000, Jiangxi, China. zhhpxjh@126.com. ;
期刊:Downregulation of circ_0035292 Alleviates LPS-Induced WI-38 Cell Injury via Targeting miR-494-3p/TLR4 Pathway in Infantile Pneumonia
DOI:10.1007/s10528-023-10455-0
影响因子 :2.4
引用产品: WI-38 细胞
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Circular RNA circESPL1 knockdown alleviates lipopolysaccharide (LPS)-induced lung cell injury via sponging miR-326 to regulate MAPK14 (2022-08-26)
作者:Yamei Liang:1.Department of Pediatrics, Taizhou First People's Hospital, China. ; Yingying Miao:1.Department of Pediatrics, Taizhou First People's Hospital, China. ; Jingjing Xiang:1.Department of Pediatrics, Taizhou First People's Hospital, China. Electronic address: vastmind@163.com. ;
期刊:Circular RNA circESPL1 knockdown alleviates lipopolysaccharide (LPS)-induced lung cell injury via sponging miR-326 to regulate MAPK14
DOI:10.1016/j.intimp.2022.109146
影响因子 :5.7
引用产品: WI-38 细胞 , MRC-5 细胞 , WI-38细胞专用培养基 , MRC-5细胞专用培养基
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Downregulation of the Circadian Rhythm Regulator HLF Promotes Multiple-Organ Distant Metastases in Non-Small Cell Lung Cancer Through PPAR NF-κb Signaling (2007-00-20)
作者:Jiarong Chen:1.Clinical Experimental Center, Jiangmen Key Laboratory of Clinical Biobanks and Translational Research, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen University, Jiangmen, 529030, China; Department of Oncology, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen University, Jiangmen, 529030, China. ; Aibin Liu:1.Department of Geriatrics, Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, 410008, China. ; Zhichao Lin:1.Department of Thoracic Surgery, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen University, Jiangmen, 529030, China. ; Bin Wang:1.Clinical Experimental Center, Jiangmen Key Laboratory of Clinical Biobanks and Translational Research, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen University, Jiangmen, 529030, China;
期刊:Downregulation of the Circadian Rhythm Regulator HLF Promotes Multiple-Organ Distant Metastases in Non-Small Cell Lung Cancer Through PPAR NF-κb Signaling
DOI:10.1016/j.canlet.2020.04.007
影响因子 :9.7
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