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产品概述
名称 | DC2.4 (小鼠骨髓来源树突状细胞) (STR鉴定正确) |
别称 | DC 2.4 |
种属 | 小鼠 |
生长特性 | 贴壁细胞,少量悬浮 |
细胞形态 | 圆形;梭形,胞突呈分枝状 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3天/次 |
注意事项 | 圆形贴壁,少量贴壁细胞伸展开,少量悬浮。密度低时漂浮较少,密度高时,漂浮比例增大,漂浮部分也可以培养。 |
背景描述 | DC2.4是通过用表达小鼠粒细胞-巨噬细胞-CSF(GM-CSF)和myc和raf致癌基因的逆转录病毒载体转导C57BL/6小鼠的骨髓分离物而产生的永生化小鼠树突状细胞。DC2.4表现出树突细胞的特征,包括细胞形态和树突细胞特异性标记物的表达,以及吞噬MHC I类和II类分子并在其上呈递外源性抗原的能力。 |
细胞类型 | 转化细胞系 |
生物安全等级 | BSL-1 |
STR鉴定
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STR位点信息
18-3 15,16 6-7 17 5-5 17,18 X-1 28 1-2 19 7-1 26.2,27.2 8-1 16 1-1 17 19-2 13 15-3 22.3,23.3 12-1 17 6-4 18 4-2 20.3,21.3 3-2 14 2-1 16 13-1 17.1
11-2 16,17 17-2 16 -
STR鉴定图
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参考文献
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Nanoadjuvant-triggered STING activation evokes systemic immunotherapy for repetitive implant-related infections (2024-01-24)
期刊:Bioactive Materials
DOI:10.1016/j.bioactmat.2024.01.020
影响因子 :18.9
引用产品: DC2.4 细胞 , 小鼠骨髓树突状细胞 (未成熟DC细胞)
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Tailoring therapeutics via a systematic beneficial elements comparison between photosynthetic bacteria-derived OMVs and extruded nanovesicles (2024-02-26)
期刊:Bioactive Materials
DOI:10.1016/j.bioactmat.2024.02.025
影响因子 :18.9
引用产品: DC2.4 细胞
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Cancer cell membrane proteins-encapsulated nanovaccine enhances cancer immunotherapy and prevention by provoking antigen-specific cellular immunity via the dendritic cell-targeted delivery (2024-01-06)
期刊:CHEMICAL ENGINEERING JOURNAL
影响因子 :15.1
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Pre-Induced ICD Membrane-Coated Carrier-Free Nanoparticles for the Personalized Lung Cancer Immunotherapy (2023-03-18)
期刊:Small Methods
影响因子 :12.4
引用产品: DC2.4 细胞
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Endogenous/Exogenous Nanovaccines Synergistically Enhance Dendritic Cell-Mediated Tumor Immunotherapy (2023-02-18)
期刊:Advanced Healthcare Materials
影响因子 :10.0
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Delivery of Mrna Vaccine with 1, 2-diesters-derived Lipids Elicits Fast Liver Clearance for Safe and Effective Cancer Immunotherapy. (2023-11-21)
期刊:Advanced Healthcare Materials
影响因子 :10.0
引用产品: B16 细胞 , RM-1 细胞 , DC2.4 细胞 , 293 [HEK-293] 细胞
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PTT/ PDT-induced microbial apoptosis and wound healing depend on immune activation and macrophage phenotype transformation (2023-06-25)
期刊:Acta Biomaterialia
DOI:10.1016/j.actbio.2023.06.025
影响因子 :9.7
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Extracellular vesicles hybrid plasmid-loaded lipid nanovesicles for synergistic cancer immunotherapy (2023-10-27)
期刊:Materials Today Bio
DOI:10.1016/j.mtbio.2023.100845
影响因子 :8.2
引用产品: DC2.4 细胞
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Doxorubicin-Loaded Platelet Decoys for Enhanced Chemoimmunotherapy Against Triple-Negative Breast Cancer in Mice Model (2023-06-30)
期刊:International Journal of Nanomedicine
影响因子 :8.0
引用产品: 特级胎牛血清 , RPMI-1640 培养基 , DC2.4 细胞 , MDA-MB-231 细胞
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Cascade catalysis-coordinated nanorobot toward synergistic cancer chemoimmunotherapy (2023-08-30)
期刊:Journal of Materials Chemistry B
影响因子 :7.0
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Interferon-γ regulates immunosuppression in septic mice by promoting the Warburg effect through the PI3K/AKT/mTOR pathway (2023-07-11)
期刊:MOLECULAR MEDICINE
DOI:10.1186/s10020-023-00690-x
影响因子 :5.7
引用产品: DC2.4 细胞 , L-谷氨酰胺溶液 (200mM),100× , 青霉素-链霉素溶液 (双抗),100× , MEM非必需氨基酸溶液 (NEAA),100× , β-巯基乙醇 (55mM),1000× , 特级胎牛血清
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Resveratrol-loaded macrophage exosomes alleviate multiple sclerosis through targeting microglia (2022-12-15)
期刊:JOURNAL OF CONTROLLED RELEASE
DOI:10.1016/j.jconrel.2022.12.026
影响因子 :11.5
引用产品: RAW 264.7 细胞 , DC2.4 细胞 , CTLL-2 细胞 , CTLL-2细胞专用培养基 , BV2 细胞 , U-87 MG 细胞
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Dendritic cells originating exosomal miR-193b-3p induces regulatory T cells to alleviate liver transplant rejection (2022-12-21)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2022.109541
影响因子 :5.7
引用产品: DC2.4 细胞
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Transcriptomic analysis reveal the responses of dendritic cells to VDBP (2003-02-20)
期刊:Genes & Genomics
DOI:10.1007/s13258-022-01234-z
影响因子 :2.1
引用产品: DC2.4 细胞
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