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产品概述
名称 | B16-F10 (小鼠皮肤黑色素瘤细胞) (STR鉴定正确) |
别称 | B16/F10; B16 F10; B16F10; B16 melanoma F10 |
种属 | 小鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 梭形细胞样;上皮样细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:4-1:8 |
推荐换液频率 | 2-3次/周 |
注意事项 | B16系列细胞,使用DMEM培养时可能出现黑色素快速积累,细胞不增殖或死亡的情况,该细胞正常扩增冻存请使用RPMI-1640完培,若您需要进行分泌黑色素等后续相关实验,可以在扩增冻存细胞后更换为DMEM培养来满足实验设计。 |
背景描述 | B16-F10细胞是B16-F0细胞的亚系。 |
年龄(性别) | 不详 |
组织来源 | 器官:皮肤;品系:C57BL/6J;疾病:黑色素瘤 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 黑色素瘤细胞 |
生物安全等级 | BSL-1 |
保藏机构 | ATCC; CRL-6475 |
STR鉴定
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STR位点信息
18-3 15,16 6-7 15 5-5 16,20 X-1 28 1-2 19,20 7-1 26.2 8-1 16,17 1-1 17,18,19 19-2 13,14 15-3 22.3,23.3 12-1 17,18 6-4 18,19 4-2 20.3,21.3 3-2 14,15 2-1 16 13-1 17.1,18.1
11-2 16,17 17-2 15,16,17 -
STR鉴定图
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参考文献
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Engineered extracellular vesicles enable high-efficient delivery of intracellular therapeutic proteins (2024-03-22)
期刊:Protein & Cell
影响因子 :21.1
引用产品: MC38 细胞 , B16-F10 细胞 , THP-1 细胞
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Mn-CaCO3-based nanosystem for augmented sonodynamic-chemodynamic immunotherapy via PI3K/Akt signaling pathway (2024-02-09)
期刊:CHEMICAL ENGINEERING JOURNAL
影响因子 :15.1
引用产品: 4T1 细胞 , B16-F10 细胞
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A novel mitochondria-targeting DHODH inhibitor induces robust ferroptosis and alleviates immune suppression (2024-02-27)
期刊:PHARMACOLOGICAL RESEARCH
DOI:10.1016/j.phrs.2024.107115
影响因子 :9.3
引用产品: B16-F10 细胞 , A-375 细胞 , 4T1 细胞 , Renca [RenCa] 细胞 , GL261 细胞 , U-87 MG 细胞 , SH-SY5Y [SHSY-5Y] 细胞 , U251 细胞
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Skin healthcare protection with antioxidant and anti-melanogenesis activity of polysaccharide purification from Bletilla striata (2024-02-15)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2024.130016
影响因子 :8.2
引用产品: B16-F10 细胞 , DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , 0.05%胰蛋白酶溶液 (溶于PBS) , MTT溶液 (5mg/mL) , 磷酸盐缓冲液 (PBS) 1×
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Engineered in vivo and in vitro tumor model recapitulates vasculogenic mimicry signatures in melanoma (2024-01-27)
期刊:Bioengineering & Translational Medicine
影响因子 :7.4
引用产品: B16-F10 细胞
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MRI-guided photothermal/photodynamic immune activation combined with PD-1 inhibitor for the multimodal combination therapy of melanoma and metastases (2024-03-14)
期刊:Regenerative Biomaterials
影响因子 :6.7
引用产品: Hep G2 细胞 , B16-F10 细胞
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Screening of Key Components for Melanogenesis Inhibition of Polygonum cuspidatum Extract Based on the Spectrum–Effect Relationship and Molecular Docking (2024-02-15)
期刊:MOLECULES
影响因子 :4.6
引用产品: B16-F10 细胞 , RPMI-1640 培养基
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A self-monitoring microneedle patch for light-controlled synergistic treatment of melanoma (2023-03-27)
期刊:Bioactive Materials
DOI:10.1016/j.bioactmat.2023.03.016
影响因子 :18.9
引用产品: B16-F10 细胞 , NIH/3T3 细胞
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Inhalable pH-responsive DNA tetrahedron nanoplatform for boosting anti-tumor immune responses against metastatic lung cancer (2023-08-24)
期刊:BIOMATERIALS
DOI:10.1016/j.biomaterials.2023.122283
影响因子 :14.0
引用产品: 磷酸盐缓冲液 (PBS) 1× , DMEM高糖 培养基 , RPMI-1640 培养基 , B16-F10 细胞 , RAW 264.7 细胞
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Blue light promotes vitamin C-mediated ferroptosis of melanoma through specifically upregulating transporter SVCT2 and generating Fe2+ (2023-05-31)
期刊:BIOMATERIALS
DOI:10.1016/j.biomaterials.2023.122186
影响因子 :14.0
引用产品: B16-F10 细胞 , 青霉素-链霉素溶液 (双抗),100×
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Suppression of Tumor or Host Intrinsic CMTM6 Drives Antitumor Cytotoxicity in a PD-L1–Independent Manner (2023-02-03)
期刊:Cancer Immunology Research
DOI:10.1158/2326-6066.CIR-22-0439
影响因子 :10.1
引用产品: Jurkat, Clone E6-1 细胞 , RKO 细胞 , CT26.WT 细胞 , B16-F10 细胞 , MC38 细胞 , 4T1 细胞 , LLC [LL/2; LLC1] 细胞 , Hepa 1-6 细胞 , YAC-1 细胞 , 293T [HEK-293T] 细胞 , AAV-293 细胞 , STR鉴定
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MC1R-targeted NIR-II aggregation-induced emission nanoparticles for melanoma imaging (2023-09-21)
期刊:Science China-Materials
影响因子 :8.1
引用产品: B16-F10 细胞
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Characterization and screening of anti-melanogenesis and anti-photoaging activity of different enzyme-assisted polysaccharide extracts from Portulaca oleracea L. (2023-05-13)
期刊:PHYTOMEDICINE
DOI:10.1016/j.phymed.2023.154879
影响因子 :7.9
引用产品: Hacat 细胞 , B16-F10 细胞
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M1 polarization enhances the antitumor activity of chimeric antigen receptor macrophages in solid tumors (2023-03-28)
期刊:Journal of Translational Medicine
DOI:10.1186/s12967-023-04061-2
影响因子 :7.4
引用产品: MC38 细胞 , B16-F10 细胞 , ID8 细胞 , 293T [HEK-293T] 细胞 , J774A.1 细胞
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RNF8 enhances the sensitivity of PD-L1 inhibitor against melanoma through ubiquitination of galectin-3 in stroma (2023-06-30)
期刊:Cell Death Discovery
DOI:10.1038/s41420-023-01500-3
影响因子 :7.0
引用产品: A-375 细胞 , A549 [A-549] 细胞 , B16-F10 细胞 , 293T [HEK-293T] 细胞
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Metabolites 13,14-Dihydro-15-keto-PGE2 Participates in Bifidobacterium animalis F1-7 to Alleviate Opioid-Induced Constipation by 5-HT Pathway (2023-12-06)
期刊:MOLECULAR NUTRITION & FOOD RESEARCH
影响因子 :5.2
引用产品: B16-F10 细胞 , B16-F10细胞专用培养基
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Linear Peptide-Based PET Tracers for Imaging PD-L1 in Tumors (2023-06-27)
期刊:MOLECULAR PHARMACEUTICS
DOI:10.1021/acs.molpharmaceut.3c00382
影响因子 :4.9
引用产品: B16-F10 细胞 , MC38 细胞
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Fractionation of Simulated Digestive Products of Bird’s Nest In Vitro: Skin Whitening Activity of Fractions (2023-08-25)
期刊:JOURNAL OF FOOD BIOCHEMISTRY
影响因子 :4.0
引用产品: RPMI-1640 培养基 , 特级胎牛血清 , Dulbecco's磷酸盐缓冲液 (DPBS),不含钙、镁、酚红 , 0.25%胰蛋白酶溶液 (含EDTA,溶于PBS) , B16-F10 细胞
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Thermosensitive Polymer Conjugated Prodrug-Activating Enzyme with Enhanced Tumor Retention and Antitumor Efficacy (2022-10-20)
期刊:BIOMACROMOLECULES
DOI:10.1021/acs.biomac.2c01006
影响因子 :7.0
引用产品: B16-F10 细胞
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Effects of novel cellulase (Cel 906) and probiotic yeast fermentation on antioxidant and anti-inflammatory activities of vine tea (Ampelopsis grossedentata) (2022-09-15)
期刊:Frontiers in Bioengineering and Biotechnology
DOI:10.3389/fbioe.2022.1006316
影响因子 :6.1
引用产品: DMSO , RPMI-1640 培养基 , 特级胎牛血清 , MTT溶液 (5mg/mL) , B16-F10 细胞
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Bifidobacterium animalis sup F1-7 Acts as an Effective Activator to Regulate Immune Response Via Casepase-3 and Bak of FAS/CD95 Pathway (2022-08-22)
期刊:Probiotics and Antimicrobial Proteins
DOI:10.1007/s12602-022-09975-9
影响因子 :5.3
引用产品: B16-F10 细胞 , DMEM高糖 培养基 , 特级胎牛血清
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Identification and characterization of two novel non-coding Tyrosinase (TYR) gene variants leading to Oculocutaneous albinism type 1 (2004-02-20)
期刊:JOURNAL OF BIOLOGICAL CHEMISTRY
影响因子 :4.8
引用产品: 293T [HEK-293T] 细胞 , A-375 细胞 , B16-F10 细胞
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Neospora caninum inhibits tumor development by activating the immune response and destroying tumor cells in a B16F10 melanoma model (2022-09-23)
期刊:Parasites & Vectors
DOI:10.1186/s13071-022-05456-8
影响因子 :4.0
引用产品: B16-F10 细胞
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Licorice zinc suppresses melanogenesis via inhibiting the activation of P38MAPK and JNK signaling pathway in Balb/c mice skin (2022-12-19)
期刊:Acta Cirurgica Brasileira
影响因子 :1.6
引用产品: B16-F10 细胞
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Establishment of tumor inflammasome clusters with distinct immunogenomic landscape aids immunotherapy (2010-01-20)
期刊:Theranostics
影响因子 :12.4
引用产品: B16-F10 细胞
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Enzyme Responsiveness Enhances the Specificity and Effectiveness ofNanoparticles for the Treatment of B16F10 Melanoma (2011-09-20)
期刊:Journal Of Controlled Release
DOI:10.1016/j.jconrel.2019.10.052
影响因子 :10.8
引用产品: B16-F10 细胞
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Targeting Angiogenesis by Blocking the ATM–SerRS–VEGFA Pathway for UV-Induced Skin Photodamage and Melanoma Growth (2011-09-20)
期刊:Cancers
影响因子 :5.2
引用产品: Hacat 细胞 , B16-F10 细胞
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Nonspecific immunoglobulin G is effective in preventing and treating cancer in mice (2003-09-20)
期刊:Cancer Management and Research
影响因子 :3.3
引用产品: B16-F10 细胞
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Nanotechnology-mediated immunochemotherapy combined with docetaxel and PD-L1 antibody increase therapeutic effects anddecrease systemic toxicity (2008-08-20)
期刊:Journal Of Controlled Release
DOI:10.1016/j.jconrel.2018.08.011
影响因子 :10.8
引用产品: B16-F10 细胞
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