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产品概述
名称 | HL-60 (人原髓细胞白血病细胞) (STR鉴定正确) |
别称 | HL 60; HL60 |
种属 | 人 |
生长特性 | 悬浮细胞 |
细胞形态 | 原粒细胞 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 3×10^5-1×10^6cells/mL |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。 |
背景描述 | HL-60细胞是一株早幼粒细胞;HL-60细胞源自一位患有急性粒-单核细胞白血病的36岁白人女性。HL-60细胞自发分化,丁酸盐、次黄嘌呤、佛波醇、肉豆蔻酸、DMSO(1%-1.5%)、放线菌素D和视黄酸均可以促进分化。HL-60细胞表现出吞噬活性,并对趋化刺激有响应;HL-60细胞致癌基因myc表达阳性。 |
年龄(性别) | 女性;36岁 |
组织来源 | 外周血;早幼粒细胞;急性粒-单核细胞白血病 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 白血病细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~28-40 hours |
致瘤性 | Yes, in nude mice (subcutaneous myeloid tumors).Yes, in semi-solid media. |
受体表达情况 | complement, expressed;Fc, expressed |
基因表达情况 | tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid, myc+ |
保藏机构 | ATCC; CCL-240 BCRC; 60027 BCRJ; 0104 DSMZ; ACC-3 ECACC; 98070106 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 13,14 D2S1338 17 D3S1358 16 D5S818 12 D7S820 11,12 D8S1179 12,13 D13S317 8,11 D16S539 11 D18S51 14,15 D19S433 14 D21S11 29,30 FGA 21,24 PentaD 10,12 PentaE 13,14 TH01 7,8 TPOX 8,11 vWA 16 D6S1043 12 D12S391 18,20 D2S441 10,14 -
STR鉴定图
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参考文献
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NFE2-driven neutrophil polarization promotes pancreatic cancer liver metastasis progression (2025-01-18)
期刊:Cell Reports
DOI:10.1016/j.celrep.2024.115226
影响因子 :7.5
引用产品: IMDM 培养基 , HL-60 细胞 , 293T [HEK-293T] 细胞 , MIA PaCa-2 细胞 , RPMI-1640 培养基 , 磷酸盐缓冲液(PBS) 1×
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Glycyrrhizic acid reduces neutrophil extracellular trap formation to ameliorate colitis-associated colorectal cancer by inhibiting peptidyl arginine deiminase 4 (2025-01-07)
期刊:JOURNAL OF ETHNOPHARMACOLOGY
影响因子 :4.8
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Neutrophil extracellular traps (NETs) are increased in rheumatoid arthritis-associated interstitial lung disease (2025-01-22)
期刊:RESPIRATORY RESEARCH
DOI:10.1186/s12931-025-03111-1
影响因子 :4.7
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N6-methyladenosine-modified SENP1, identified by IGF2BP3, is a novel molecular marker in acute myeloid leukemia and aggravates progression by activating AKT signal via de-SUMOylating HDAC2 (2024-05-31)
期刊:Molecular Cancer
DOI:10.1186/s12943-024-02013-y
影响因子 :27.7
引用产品: 293T [HEK-293T] 细胞 , HL-60 细胞 , THP-1 细胞 , NB4 细胞 , DMEM高糖完全培养基(含10% FBS) , IMDM完全培养基(含10% FBS) , RPMI-1640完全培养基(含10% FBS)
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Neutrophil ALDH2 is a new therapeutic target for the effective treatment of sepsis-induced ARDS (2024-03-12)
期刊:Cellular & Molecular Immunology
DOI:10.1038/s41423-024-01146-w
影响因子 :24.1
引用产品: HL-60 细胞
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Ozone-delivering nanocomposite hydrogel for acute Staphylococcus aureus osteomyelitis treatment via neutrophil regulation (2024-04-10)
期刊:CHEMICAL ENGINEERING JOURNAL
影响因子 :15.1
引用产品: HL-60 细胞
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A cationic hydrogel with anti-IL-17A-specific nanobodies for rheumatoid arthritis treatment via inhibition of inflammatory activities of neutrophils (2024-09-20)
期刊:Nano Today
DOI:10.1016/j.nantod.2024.102507
影响因子 :13.2
引用产品: HL-60 细胞 , RAW 264.7 细胞
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Macrophage Tim-3 maintains intestinal homeostasis in DSS-induced colitis by suppressing neutrophil necroptosis (2024-02-02)
期刊:Redox Biology
DOI:10.1016/j.redox.2024.103072
影响因子 :11.4
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A PET Probe Targeting Polyamine Transport System for Precise Tumor Diagnosis and Therapy (2024-04-25)
期刊:Asian Journal of Pharmaceutical Sciences
DOI:10.1016/j.ajps.2024.100924
影响因子 :10.2
引用产品: HL-60 细胞 , SK-OV-3 细胞
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Esculetin facilitates post-stroke rehabilitation by inhibiting CKLF1-mediated neutrophil infiltration (2024-08-07)
期刊:ACTA PHARMACOLOGICA SINICA
DOI:10.1038/s41401-024-01352-2
影响因子 :6.9
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Immune infiltration-related genes regulate the progression of AML by invading the bone marrow microenvironment (2024-07-12)
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2024.1409945
影响因子 :5.7
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Assessing immune hepatotoxicity of troglitazone with a versatile liver-immune-microphysiological-system (2024-05-30)
期刊:Frontiers in Pharmacology
DOI:10.3389/fphar.2024.1335836
影响因子 :5.6
引用产品: HL-60 细胞 , NK-92 细胞 , NK-92细胞专用培养基
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Targeting protein tyrosine phosphatase non-receptor type 6 (PTPN6) as a therapeutic strategy in acute myeloid leukemia (2024-12-21)
期刊:CELL BIOLOGY AND TOXICOLOGY
DOI:10.1007/s10565-024-09965-3
影响因子 :5.3
引用产品: HL-60 细胞
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GANT61 surmounts drug resistance of ADR by upregulating lysosome activities and reducing BCL2 expression in HL-60/ADR cells (2024-12-26)
期刊:Cancer Cell International
DOI:10.1186/s12935-024-03626-5
影响因子 :5.3
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Sequential Immune Acquisition of Monoclonal Antibodies Enhances Phagocytosis of Acinetobacter baumannii by Recognizing ATP Synthase (2024-09-29)
期刊:Vaccines
影响因子 :5.2
引用产品: HL-60 细胞
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Neutrophil PPIF exacerbates lung ischemia–reperfusion injury after lung transplantation by promoting calcium overload-induced neutrophil extracellular traps formation (2024-09-04)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2024.113051
影响因子 :4.8
引用产品: HL-60 细胞
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Neutrophil-to-lymphocyte ratio in colorectal tissue affects prognosis in patients with colorectal cancer (2024-05-15)
期刊:PATHOLOGY
DOI:10.1016/j.pathol.2024.03.003
影响因子 :4.5
引用产品: HL-60 细胞 , HCT 116 细胞 , LoVo 细胞 , HT-29 细胞 , H9 [derivative of HuT 78] 细胞
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Identifying PTAFR as a hub gene in atherosclerosis: implications for NETosis and disease progression (2024-12-21)
期刊:Human Genomics
DOI:10.1186/s40246-024-00708-3
影响因子 :3.8
引用产品: HL-60 细胞
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Curcumin attenuates lupus nephritis by inhibiting neutrophil migration via PI3K/AKT/NF-κB signalling pathway (2024-07-01)
期刊:Lupus Science & Medicine
影响因子 :3.7
引用产品: HL-60 细胞
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Activation of non-classical Wnt signaling pathway effectively enhances HLA-A presentation in acute myeloid leukemia (2024-06-19)
期刊:Frontiers in Oncology
影响因子 :3.5
引用产品: MOLM-13 细胞 , 人骨髓造血干细胞完全培养基 , 人骨髓造血干细胞 , HL-60 细胞 , THP-1 细胞
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ZNF521 promotes acute myeloid leukemogenesis by suppressing the expression and acetylation of SMC3 (2024-09-06)
期刊:Heliyon
DOI:10.1016/j.heliyon.2024.e37528
影响因子 :3.4
引用产品: THP-1 细胞 , HL-60 细胞 , 293T [HEK-293T] 细胞
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Correlating gene expression levels with transcription factor binding sites facilitates identification of key transcription factors from transcriptome data (2024-11-29)
期刊:Frontiers in Genetics
DOI:10.3389/fgene.2024.1511456
影响因子 :2.8
引用产品: MOLM-13 细胞 , HL-60 细胞 , U-937 细胞 , OCI-AML-3 细胞
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NAP1L5 promotes epithelial–mesenchymal transition by regulating PEG10 expression in acute myeloid leukaemia (2024-11-13)
期刊:LEUKEMIA RESEARCH
DOI:10.1016/j.leukres.2024.107623
影响因子 :2.1
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High STAT4 expression correlates with poor prognosis in acute myeloid leukemia and facilitates disease progression by upregulating VEGFA expression (2024-05-09)
期刊:Open Medicine
影响因子 :2.1
引用产品: KG-1 细胞 , HL-60 细胞 , Kasumi-1 细胞 , HUV-EC-C [HUVEC]细胞专用培养基 , HL-60细胞专用培养基 , NB4细胞专用培养基 , Kasumi-1细胞专用培养基 , NB4 细胞 , HUV-EC-C [HUVEC] 细胞
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CREB1-driven CXCR4hi neutrophils promote skin inflammation in mouse models and human patients (2023-09-22)
期刊:Nature Communications
DOI:10.1038/s41467-023-41484-3
影响因子 :16.6
引用产品: HL-60 细胞
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Oligomer-guided recognition of two fucan sulfate from Bohadschia argus and inhibition of P-selectin binding to its ligand (2023-06-01)
期刊:CARBOHYDRATE POLYMERS
DOI:10.1016/j.carbpol.2023.121080
影响因子 :11.2
引用产品: HL-60 细胞
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METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling. (2023-03-14)
期刊:Cell Reports
DOI:10.1016/j.celrep.2023.112259
影响因子 :8.8
引用产品: HL-60 细胞 , 293T/17 细胞
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Multifunctional targetable liposomal drug delivery system in the management of leukemia: Potential, opportunities, and emerging strategies (2023-05-13)
期刊:LIFE SCIENCES
影响因子 :6.1
引用产品: HL-60 细胞 , K-562 [K562] 细胞
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Low-dose ethanol consumption inhibits neutrophil extracellular traps formation to alleviate rheumatoid arthritis (2023-10-26)
期刊:Communications Biology
DOI:10.1038/s42003-023-05473-y
影响因子 :5.9
引用产品: HL-60 细胞
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Multiomic landscape of immune pathogenesis in Kimura's disease (2023-04-01)
期刊:iScience
DOI:10.1016/j.isci.2023.106559
影响因子 :5.8
引用产品: HL-60 细胞
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Acetylation stabilizes the signaling protein WISP2 by preventing its degradation to suppress the progression of acute myeloid leukemia (2023-01-31)
期刊:JOURNAL OF BIOLOGICAL CHEMISTRY
影响因子 :4.8
引用产品: HL-60 细胞 , Kasumi-1 细胞 , KG-1 细胞
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Naringenin induces the cell apoptosis of acute myeloid leukemia cells by regulating the lncRNA XIST/miR-34a/HDAC1 signaling (2023-05-03)
期刊:Heliyon
DOI:10.1016/j.heliyon.2023.e15826
影响因子 :4.0
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Roles of the hsa_circ_0013880/USP32/Rap1b axis in the proliferation and apoptosis of acute myeloid leukemia cells: hsa_circ_0013880/USP32/Rap1b in acute myeloid leukemia (2023-03-23)
期刊:ACTA BIOCHIMICA ET BIOPHYSICA SINICA
影响因子 :3.7
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Role of Lipocalin-2 in N1/N2 Neutrophil Polarization After Stroke (2023-05-29)
期刊:CNS & Neurological Disorders-Drug Targets
DOI:10.2174/1871527322666230417112850
影响因子 :3.0
引用产品: HL-60 细胞
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Hyposterolactone A, a 3α-Hydroxy Steroidal Lactone from the Deep-Sea-Derived Fungus Hypocrea sp. ZEN14 (2023-06-02)
期刊:CHEMISTRY & BIODIVERSITY
影响因子 :2.9
引用产品: HuH-7 细胞 , Hep G2 细胞 , HL-60 细胞 , Jurkat, Clone E6-1 细胞
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CircPLXNB2 regulates acute myeloid leukemia progression through miR-654-3p/CCND1 axis (2023-06-05)
期刊:Hematology
DOI:10.1080/16078454.2023.2220522
影响因子 :1.9
引用产品: HL-60 细胞 , Kasumi-1 细胞 , 293T [HEK-293T] 细胞
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Artesunate reverses cytarabine resistance in acute myeloid leukemia by blocking the JAK/STAT3 signaling (2023-10-10)
期刊:Hematology
DOI:10.1080/16078454.2023.2255802
影响因子 :1.9
引用产品: Annexin V-FITC/PI荧光双染细胞凋亡检测试剂盒 , HSPC-1 细胞 , HL-60 细胞
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Circ_0001602 aggravates the progression of acute myeloid leukemia by regulating the miR-192-5p/ZBTB20 axis (2023-08-10)
期刊:Hematology
DOI:10.1080/16078454.2023.2240133
影响因子 :1.9
引用产品: THP-1 细胞 , HL-60 细胞 , NB4 细胞 , 293T [HEK-293T] 细胞 , DMEM高糖 培养基 , 特级胎牛血清 , IMDM 培养基 , RPMI-1640 培养基
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The m6A reader IGF2BP3 promotes acute myeloid leukemia progression by enhancing RCC2 stability (2022-02-25)
期刊:Experimental And Molecular Medicine
DOI:10.1038/s12276-022-00735-x
影响因子 :12.8
引用产品: HL-60 细胞
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Neural network-enhanced real-time impedance flow cytometry for single-cell intrinsic characterization (2022-01-18)
期刊:LAB ON A CHIP
影响因子 :6.1
引用产品: A549 [A-549] 细胞 , MCF7 [MCF-7] 细胞 , Hela 细胞 , HL-60 细胞
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RAB3D/MDM2/β-catenin/c-MYC axis exacerbates the malignant behaviors of acute myeloid leukemia cells in vitro and in vivo (2022-10-24)
期刊:CANCER GENE THERAPY
DOI:10.1038/s41417-022-00549-z
影响因子 :5.9
引用产品: HL-60 细胞 , U-937 细胞 , Kasumi-1 细胞 , KG-1 细胞
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Comprehensive analysis of ferroptosis-related gene signatures as a potential therapeutic target for acute myeloid leukemia: A bioinformatics analysis and experimental verification (2022-08-11)
期刊:Frontiers in Oncology
影响因子 :5.7
引用产品: Kasumi-1 细胞 , HL-60 细胞
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LncRNA CD27-AS1 promotes acute myeloid leukemia progression through the miR-224-5p/PBX3 signaling circuit (2021-05-18)
期刊:Cell Death & Disease
DOI:10.1038/s41419-021-03767-9
影响因子 :9.0
引用产品: Kasumi-1 细胞 , KG-1 细胞 , HL-60 细胞 , IMDM 培养基 , RPMI-1640 培养基
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Estrone-targeted liposomes for mitoxantrone delivery via estrogen receptor: In vivo targeting efficacy, antitumor activity, acute toxicity and pharmacokinetics (2021-03-02)
期刊:European Journal Of Pharmaceutical Sciences
DOI:10.1016/j.ejps.2021.105780
影响因子 :4.6
引用产品: HL-60 细胞
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A Novel Phenoxazine-based Fluorescent Probe for the Detection of HOCl in Living Cells (2020-05-05)
期刊:Zeitschrift Fur Anorganische Und Allgemeine Chemie
影响因子 :1.4
引用产品: RAW 264.7 细胞 , HL-60 细胞
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Oestrone‐targeted liposomes for mitoxantrone delivery via oestrogen receptor – synthesis, physicochemical characterization and in‐vitro evaluation (2017-04-26)
期刊:Journal of Pharmacy and Pharmacology
影响因子 :3.3
引用产品: HL-60 细胞
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