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产品概述
| 名称 | NK-92 (人恶性非霍奇金淋巴瘤患者的自然杀伤细胞) (STR鉴定正确) |
| 别称 | NK92; Natural Killer-92; NK-92.05; Neukoplast; aNK |
| 种属 | 人类 |
| 生长特性 | 悬浮细胞 |
| 细胞形态 | 淋巴母细胞样 |
| 冻存条件 | 冻存液：90%FBS+10%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
MEMα＋0.2mM Inositol＋0.1mM β-mercaptoethanol＋0.02mM Folic Acid＋100-200U/mL recombinant IL-2＋12.5% HS＋12.5% FBS＋1% P/S
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
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| 推荐传代比例 | 5×10^5-1×10^6cells/mL |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 该细胞为悬浮细胞，请注意离心收集细胞悬液；请勿直接倒掉细胞培养液。 |
| 背景描述 | NK-92细胞是从一位患有急进性非霍奇金淋巴瘤的50岁白人男性外周血单核细胞衍生来的一株IL-2依赖型NK细胞株。NK-92细胞对很多恶性细胞有细胞毒性；铬释放试验显示它能杀死K562细胞和Daudi细胞。NK-92细胞(经过照射以防止增殖)可以有效地用于血液中白血病的体外免疫清扫而不危及血细胞的功能。NK-92细胞有以下特征：CD2、CD7、CD11a、CD28、CD45、CD54表面标记阳性；CD1、CD3、CD4、CD5、CD8、CD10、CD14、CD16、CD19、CD20、CD23、CD34和HLA-DR表面标记阴性。 |
| 年龄（性别） | 男性；50岁 |
| 组织来源 | 外周血 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 淋巴瘤细胞 |
| 生物安全等级 | BSL-2 |
| 倍增时间 | ~40-50 hours |
| 保藏机构 | ATCC; CRL-2407 DSMZ; ACC-488 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 19,20 D3S1358 15 D5S818 12,13 D7S820 10,11 D8S1179 12 D13S317 9,12 D16S539 11,12 D18S51 12,17 D19S433 14,15 D21S11 31.2,32 FGA 20,22 PentaD 10,12 PentaE 12 TH01 6,9.3 TPOX 8 vWA 18 D6S1043 11,12 D12S391 19 D2S441 14 -
STR鉴定图
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参考文献
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NK92 cells and peripheral blood NK cells respond oppositely upon dasatinib treatment (2024-02-15)
作者：Fengqi Li:1.School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China. ; 2.Tianjin University and Health-Biotech United Group Joint Laboratory of Innovative Drug Development and Translational Medicine, Tianjin University, Tianjin, China. ; Zhongyi Wang:1.School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China. ; 2.Tianjin University and Health-Biotech United Group Joint Laboratory of Innovative Drug Development and Translational Medicine, Tianjin University, Tianjin, China. ; Dongpeng Zheng:1.School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China. ; 2.Tianjin University and Health-Biotech United Group Joint Laboratory of Innovative Drug Development and Translational Medicine, Tianjin University, Tianjin, China. ; Zhaojun Pang:1.School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China. ; 2.Tianjin University and Health-Biotech United Group Joint Laboratory of Innovative Drug
期刊：NK92 cells and peripheral blood NK cells respond oppositely upon dasatinib treatment
影响因子 ：6.4
引用产品： 青霉素-链霉素溶液 (双抗)，100× , NK-92 细胞 , MEMα (不含核苷) 培养基 , IL-2 (重组人白细胞介素-2、白细胞介素-2) (250μL×4) , 马血清 , 特级胎牛血清
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Enhanced NK cell activation via eEF2K-mediated potentiation of the cGAS–STING pathway in hepatocellular carcinoma (2024-02-05)
作者：Yan Xu:1.Medical School of Chinese PLA, Beijing, China; Faculty of Hepato-Pancreato-Biliary Surgery, the First Medical Center, Chinese PLA General Hospital, Institute of Hepatobiliary Surgery of Chinese PLA, Key Laboratory of Digital Hepatobiliary Surgery, PLA, Beijing, China. ; Fang Sun:1.Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China. ; Yuying Tian:1.Faculty of Hepato-Pancreato-Biliary Surgery, the First Medical Center, Chinese PLA General Hospital, Institute of Hepatobiliary Surgery of Chinese PLA, Key Laboratory of Digital Hepatobiliary Surgery, PLA, Beijing, China. ; Guineng Zeng:1.Faculty of Hepato-Pancreato-Biliary Surgery, the First Medical Center, Chinese PLA General Hospital, Institute of Hepatobiliary Surgery of Chinese PLA, Key Laboratory of Digital Hepatobiliary Surgery, PLA, Beijing, China. ; Guanglin Lei:1.Senior Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospi
期刊：Enhanced NK cell activation via eEF2K-mediated potentiation of the cGAS–STING pathway in hepatocellular carcinoma
DOI：10.1016/j.intimp.2024.111628
影响因子 ：5.6
引用产品： NK-92 细胞 , HCCLM3 细胞 , 人源NK细胞完全培养基
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A biomimetic liver cancer on-a-chip reveals a critical role of LIPOCALIN-2 in promoting hepatocellular carcinoma progression (2023-05-04)
作者：Peiliang Shen:1.Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, School of Medicine & Holistic Integrative Medicine, Jiangsu Joint International Research Laboratory of Chinese Medicine and Regenerative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. ; Yuanyuan Jia:1.Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, School of Medicine & Holistic Integrative Medicine, Jiangsu Joint International Research Laboratory of Chinese Medicine and Regenerative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. ; Weijia Zhou:1.Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, School of Medicine & Holistic Integrative Medicine, Jiangsu Joint International Research Laboratory of Chinese Medicine and Regenerative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. ; Weiwei Zheng:1.Jiangsu Key Laboratory for Pharmac
期刊：A biomimetic liver cancer on-a-chip reveals a critical role of LIPOCALIN-2 in promoting hepatocellular carcinoma progression
DOI：10.1016/j.apsb.2023.04.010
影响因子 ：14.5
引用产品： LX-2 细胞 , 人脐静脉内皮细胞 , NK-92 细胞 , NK-92细胞专用培养基
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L-kynurenine induces NK cell loss in gastric cancer microenvironment via promoting ferroptosis (2023-03-01)
作者：Jian-Xin Cui:1.Department of General Surgery, The First Medical Center, Chinese PLA General Hospital, Beijing, 100853, China. ; Xian-Hui Xu:1.Department of Emergency, No. 971 Hospital of PLAN, Qingdao, 266071, Shandong Province, China. ; Tao He:1.Department of General Surgery and Center of Minimal Invasive Gastrointestinal Surgery, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China. ; Jia-Jia Liu:1.Department of General Surgery and Center of Minimal Invasive Gastrointestinal Surgery, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China. ; Tian-Yu Xie:1.Department of General Surgery, The First Medical Center, Chinese PLA General Hospital, Beijing, 100853, China. ; Wen Tian:1.Department of General Surgery, The First Medical Center, Chinese PLA General Hospital, Beijing, 100853, China. tianwen301_cta01@163.com. ; Jun-Yan Liu:1.Department of General Surgery, The First Medical C
期刊：L-kynurenine induces NK cell loss in gastric cancer microenvironment via promoting ferroptosis
DOI：10.1186/s13046-023-02629-w
影响因子 ：11.3
引用产品： NK-92 细胞
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Therapeutic effects against high-grade glioblastoma mediated by engineered induced neural stem cells combined with GD2-specific CAR-NK (2023-07-07)
作者：Weihua Liu:1.Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital Capital Medical University, National Clinical Research Center for Geriatric Diseases, and Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing, 100053, China. ; 2.Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, 100069, China. ; Yu Zhao:1.Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital Capital Medical University, National Clinical Research Center for Geriatric Diseases, and Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing, 100053, China. ; 2.Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, 100069, China. ; Zhongfeng Liu:1.Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital Capital Medical University, National Clinical Research Center for Geriatric Diseases, and Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing, 10005
期刊：Therapeutic effects against high-grade glioblastoma mediated by engineered induced neural stem cells combined with GD2-specific CAR-NK
DOI：10.1007/s13402-023-00842-5
影响因子 ：6.6
引用产品： U-87 MG 细胞 , NK-92 细胞 , MEM 培养基
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SHFM1 deficiency suppresses esophageal squamous cell carcinomas progression via modulating NF‑κB signaling and enhancing nature killer cell‑mediated tumor surveillance (2023-03-17)
作者：Yijuan Wu:1.Department of Oncology and Immunology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050010, P.R. China. ; 2.Endoscopy Center, Xingtai People's Hospital, Xingtai, Hebei 054031, P.R. China. ; Zhiyu Wang:1.Department of Oncology and Immunology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050010, P.R. China. ; Shengmian Li:1.Department of Gastroenterology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050010, P.R. China. ; Xianliang Chen:1.Department of Gastroenterology, Xingtai People's Hospital, Xingtai, Hebei 054031, P.R. China. ; Shengyun Zhou:1.Endoscopy Center, Xingtai People's Hospital, Xingtai, Hebei 054031, P.R. China. ;
期刊：SHFM1 deficiency suppresses esophageal squamous cell carcinomas progression via modulating NF‑κB signaling and enhancing nature killer cell‑mediated tumor surveillance
影响因子 ：2.7
引用产品： KYSE-410 细胞 , NK-92 细胞 , NK‑92细胞专用培养基
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MMP-2 Inhibitor-Mediated Tumor Microenvironment Regulation Using a Sequentially Released Bio-Nanosystem for Enhanced Cancer Photo-Immunotherapy (2022-09-08)
作者：Huifang Liu:1.Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Biomedical Photonics and Sensing, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, PR China. ; Dongqin Lei:1.Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Biomedical Photonics and Sensing, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, PR China. ; Jiong Li:1.Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Biomedical Photonics and Sensing, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, PR China. ; Jing Xin:1.Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Biomedical Photonics and Sensing, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, PR China. ; Luwei Zhang:1.Key Laboratory of Biomed
期刊：MMP-2 Inhibitor-Mediated Tumor Microenvironment Regulation Using a Sequentially Released Bio-Nanosystem for Enhanced Cancer Photo-Immunotherapy
影响因子 ：10.4
引用产品： NK-92 细胞
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Natural killer cell-mimicking nanomaterial for overcoming the multidrug resistance of tumor via cascade catalysis (2022-10-14)
作者：Min-Jie Li (**敏捷): 1.Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan, 430072, China; Fan Gao (高凡): 1.Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan, 430072, China; Qian-**ao Huang (黄千笑): 1.Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan, 430072, China; Jun Feng (冯俊): 1.Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan, 430072, China; Chuan-Jun Liu (刘传军): 1.Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan, 430072, China; Shu-Ling Gong (龚淑玲): 1.Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan, 430072, China; **an-Zheng Zhang
期刊：Natural killer cell-mimicking nanomaterial for overcoming the multidrug resistance of tumor via cascade catalysis
影响因子 ：8.6
引用产品： NK-92 细胞 , MEMα (不含核苷) 培养基 , β-巯基乙醇 (55mM)，1000× , 马血清 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100×
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Enhanced Proinflammatory Cytokine Production and Immunometabolic Impairment of NK Cells Exposed to Mycobacterium tuberculosis and Cigarette Smoke (2001-02-20)
作者：Yafei Rao:1.Department of Respiratory and Critical Care Medicine, Peking University Third Hospital, Beijing, China. ; Xiaoyan Gai:1.Department of Respiratory and Critical Care Medicine, Peking University Third Hospital, Beijing, China. ; Yanqing Le:1.Department of Respiratory and Critical Care Medicine, Peking University Third Hospital, Beijing, China. ; Jing Xiong:1.Department of Respiratory and Critical Care Medicine, Peking University Third Hospital, Beijing, China. ; Yujia Liu:1.Department of Respiratory and Critical Care Medicine, Peking University International Hospital, Beijing, China. ; Xueyang Zhang:1.Department of Respiratory and Critical Care Medicine, Peking University Third Hospital, Beijing, China. ; Jundong Wang:1.Department of Infectious Diseases of Beijing Geriatric Hospital, Beijing, China. ; Wenli Cao:1.Department of Infectious Diseases of Beijing Geriatric Hospital, Beijing, China. ; Yongchang Sun:1.Department of Respiratory and Critical Care Medicine, Pekin
期刊：Enhanced Proinflammatory Cytokine Production and Immunometabolic Impairment of NK Cells Exposed to Mycobacterium tuberculosis and Cigarette Smoke
影响因子 ：5.5
引用产品： NK-92 细胞
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PKCι regulates the expression of PDL1 through multiple pathways to modulate immune suppression of pancreatic cancer cells (2008-01-20)
作者：Hongmei Zhang:1.Department of Immunology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan 610041, PR China. ; Yue Zhu:1.Department of Immunology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan 610041, PR China. ; Junli Wang:1.Department of Biochemistry, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan 610041, PR China. ; Sijia Weng:1.Department of Immunology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan 610041, PR China. ; Fengqiong Zuo:1.Department of Immunology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan 610041, PR China. ; Changlong Li:1.Department of Biochemistry, West China School of B
期刊：PKCι regulates the expression of PDL1 through multiple pathways to modulate immune suppression of pancreatic cancer cells
DOI：10.1016/j.cellsig.2021.110115
影响因子 ：4.8
引用产品： NK-92 细胞
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Transcription factor Sp1 is upregulated by PKCι to drive the expression of YAP1 during pancreatic carcinogenesis (2004-01-20)
作者：Jinhe Yang:1.Department of Immunology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, Sichuan, PR China. ; Junli Wang:1.Department of Biochemistry, West China School of Basic Medical Sciences & Forensic Medicine, Sichan University, Chengdu, Sichuan, PR China. ; Hongmei Zhang:1.Department of Immunology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, Sichuan, PR China. ; Changlong Li:1.Department of Biochemistry, West China School of Basic Medical Sciences & Forensic Medicine, Sichan University, Chengdu, Sichuan, PR China. ; Changyan Chen:1.The Center of Drug Discovery, Northeastern University, Boston, MA, USA. ; Tongbo Zhu:1.Department of Immunology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, Sichuan, PR China. ;
期刊：Transcription factor Sp1 is upregulated by PKCι to drive the expression of YAP1 during pancreatic carcinogenesis
影响因子 ：4.7
引用产品： NK-92 细胞
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CD44 3-Untranslated Region Functions as a Competing Endogenous RNA to Enhance NK Sensitivity of Liver Cancer Stem Cell by Regulating ULBP2 Expression (2006-09-20)
作者：Jun Weng:1.Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research, Southern Medical University, Guangzhou, China. ; Xu Han:1.Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research, Southern Medical University, Guangzhou, China. ; Kaiyu Liu:1.Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research, Southern Medical University, Guangzhou, China. ; Jiong Yang:1.Department of Geriatrics, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Shiruo Wei:1.Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China. ; Yue Zhang:1.Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of
期刊：CD44 3-Untranslated Region Functions as a Competing Endogenous RNA to Enhance NK Sensitivity of Liver Cancer Stem Cell by Regulating ULBP2 Expression
影响因子 ：9.2
引用产品： NK-92 细胞
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