产品中心Cell Resources
联系我们CONTACT US
400-999-210024小时服务热线
产品概述
名称 | NK-92 (人恶性非霍奇金淋巴瘤患者的自然杀伤细胞) (STR鉴定正确) |
别称 | NK92; Natural Killer-92; NK-92.05; Neukoplast; aNK |
种属 | 人类 |
生长特性 | 悬浮细胞 |
细胞形态 | 淋巴母细胞样 |
冻存条件 | 冻存液:90%FBS+10%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
MEMα+0.2mM Inositol+0.1mM β-mercaptoethanol+0.02mM Folic Acid+100-200U/mL recombinant IL-2+12.5% HS+12.5% FBS+1% P/S
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 5×10^5-1×10^6cells/mL |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。 |
背景描述 | NK-92细胞是从一位患有急进性非霍奇金淋巴瘤的50岁白人男性外周血单核细胞衍生来的一株IL-2依赖型NK细胞株。NK-92细胞对很多恶性细胞有细胞毒性;铬释放试验显示它能杀死K562细胞和Daudi细胞。NK-92细胞(经过照射以防止增殖)可以有效地用于血液中白血病的体外免疫清扫而不危及血细胞的功能。NK-92细胞有以下特征:CD2、CD7、CD11a、CD28、CD45、CD54表面标记阳性;CD1、CD3、CD4、CD5、CD8、CD10、CD14、CD16、CD19、CD20、CD23、CD34和HLA-DR表面标记阴性。 |
年龄(性别) | 男性;50岁 |
组织来源 | 外周血 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 淋巴瘤细胞 |
生物安全等级 | BSL-2 |
倍增时间 | ~40-50 hours |
保藏机构 | ATCC; CRL-2407 DSMZ; ACC-488 |
STR鉴定
-
STR位点信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 19,20 D3S1358 15 D5S818 12,13 D7S820 10,11 D8S1179 12 D13S317 9,12 D16S539 11,12 D18S51 12,17 D19S433 14,15 D21S11 31.2,32 FGA 20,22 PentaD 10,12 PentaE 12 TH01 6,9.3 TPOX 8 vWA 18 D6S1043 11,12 D12S391 19 D2S441 14 -
STR鉴定图
-
参考文献
-
Strategic and Technical Considerations in Manufacturing Viral Vector Vaccines for the Biomedical Advanced Research and Development Authority Threats (2025-01-14)
期刊:Vaccines
影响因子 :5.2
引用产品: NK-92 细胞
-
APOC1 inhibit NKTCL doxorubicin sensitivity by promoting mitophagy (2025-01-16)
期刊:IUBMB LIFE
DOI:10.1002/iub.2942
影响因子 :3.7
引用产品: NK-92 细胞 , NK-92细胞专用培养基
-
Exosomal miR-552-5p Regulates the Role of NK Cells in EMT of Gastric Cancer via the PD-1/PD-L1 Axis (2025-01-01)
期刊:Journal of Cancer
影响因子 :3.3
引用产品: NK-92 细胞
-
2D4, a humanized monoclonal antibody targeting CD132, is a promising treatment for systemic lupus erythematosus (2024-11-17)
期刊:Signal Transduction and Targeted Therapy
DOI:10.1038/s41392-024-02017-6
影响因子 :40.8
-
Targeting Caveolin-1 in Multiple Myeloma Cells Enhances Chemotherapy and Natural Killer Cell-Mediated Immunotherapy (2024-12-04)
期刊:Advanced Science
影响因子 :14.3
引用产品: NK-92 细胞 , MEMα(不含核苷) 培养基 , 特级胎牛血清 , 马血清
-
Off-the-shelf CAR-NK cells targeting immunogenic cell death marker ERp57 execute robust antitumor activity and have a synergistic effect with ICD inducer oxaliplatin (2024-07-01)
期刊:Journal for ImmunoTherapy of Cancer
影响因子 :10.3
引用产品: NK-92 细胞 , MKN-45 细胞 , Eca-109 细胞 , IM95 细胞 , 293T/17 细胞 , MOLM-13 细胞
-
Multi-omics revealed that ELAVL3 regulates MYCN in neuroblastoma via immunogenic cell death: Risk stratification and experimental research (2024-10-31)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2024.137045
影响因子 :7.7
引用产品: NK-92细胞专用培养基 , NK-92 细胞
-
NK92 cells and peripheral blood NK cells respond oppositely upon dasatinib treatment (2024-02-15)
期刊:IMMUNOLOGY
影响因子 :6.4
引用产品: 青霉素-链霉素溶液 (双抗),100× , NK-92 细胞 , MEMα (不含核苷) 培养基 , IL-2 (重组人白细胞介素-2、白细胞介素-2) (250μL×4) , 马血清 , 特级胎牛血清
-
Assessing immune hepatotoxicity of troglitazone with a versatile liver-immune-microphysiological-system (2024-05-30)
期刊:Frontiers in Pharmacology
DOI:10.3389/fphar.2024.1335836
影响因子 :5.6
引用产品: HL-60 细胞 , NK-92 细胞 , NK-92细胞专用培养基
-
Enhanced NK cell activation via eEF2K-mediated potentiation of the cGAS–STING pathway in hepatocellular carcinoma (2024-02-05)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2024.111628
影响因子 :5.6
引用产品: NK-92 细胞 , HCCLM3 细胞 , 人源NK细胞完全培养基
-
Platelet-derived major histocompatibility complex class I coating on Treponema pallidum attenuates natural killer cell lethality (2024-05-14)
期刊:Virulence
DOI:10.1080/21505594.2024.2350892
影响因子 :5.2
引用产品: NK-92 细胞
-
Co-Colorectal cancer stem cells employ the FADS1/DDA axis to evade NK cell-mediated immunosuppression after co-cultured with NK cells under hypoxia (2024-11-02)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2024.113535
影响因子 :4.8
-
Co-culture of natural killer cells and tumor spheroids on a heterogeneous multilayer paper stack (2024-12-30)
期刊:Journal of Zhejiang University-SCIENCE B
影响因子 :4.7
引用产品: NK-92 细胞 , NK-92细胞专用培养基
-
Development of TSHR-CAR NK-92 Cells for Differentiated Thyroid Cancer (2024-04-24)
期刊:MOLECULAR AND CELLULAR ENDOCRINOLOGY
影响因子 :4.1
引用产品: NK-92 细胞
-
A Pump-Free Microfluidic Co-Culture System for Investigating NK Cell-Tumor Spheroid Interactions in Flow Conditions (2024-11-15)
期刊:JOURNAL OF BIOTECHNOLOGY
DOI:10.1016/j.jbiotec.2024.11.008
影响因子 :4.1
引用产品: NK-92 细胞 , PNT1A 细胞 , NK-92细胞专用培养基 , DU 145 细胞
-
The BCL2-associated athanogene-3–Interferon-induced transmembrane protein 2 axis enhances pancreatic ductal adenocarcinoma growth via the Mitogen-activated protein kinase signaling pathway (2024-08-30)
期刊:CARCINOGENESIS
影响因子 :3.3
引用产品: MIA PaCa-2 细胞 , PANC-1 细胞 , SU.86.86 细胞 , BxPC-3 细胞 , NK-92 细胞
-
Regulation of the PD-1/PD-L1 Axis and NK Cell Dysfunction by Exosomal miR-552-5p in Gastric Cancer (2024-07-17)
期刊:DIGESTIVE DISEASES AND SCIENCES
DOI:10.1007/s10620-024-08536-0
影响因子 :2.5
引用产品: NK-92 细胞
-
A biomimetic liver cancer on-a-chip reveals a critical role of LIPOCALIN-2 in promoting hepatocellular carcinoma progression (2023-05-04)
期刊:Acta Pharmaceutica Sinica B
DOI:10.1016/j.apsb.2023.04.010
影响因子 :14.5
引用产品: LX-2 细胞 , 人脐静脉内皮细胞 , NK-92 细胞 , NK-92细胞专用培养基
-
L-kynurenine induces NK cell loss in gastric cancer microenvironment via promoting ferroptosis (2023-03-01)
期刊:JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI:10.1186/s13046-023-02629-w
影响因子 :11.3
引用产品: NK-92 细胞
-
Therapeutic effects against high-grade glioblastoma mediated by engineered induced neural stem cells combined with GD2-specific CAR-NK (2023-07-07)
期刊:CELLULAR ONCOLOGY
DOI:10.1007/s13402-023-00842-5
影响因子 :6.6
引用产品: U-87 MG 细胞 , NK-92 细胞 , MEM 培养基
-
SHFM1 deficiency suppresses esophageal squamous cell carcinomas progression via modulating NF?κB signaling and enhancing nature killer cell?mediated tumor surveillance (2023-03-17)
期刊:Experimental and Therapeutic Medicine
影响因子 :2.7
引用产品: KYSE-410 细胞 , NK-92 细胞 , NK 92细胞专用培养基
-
MMP-2 Inhibitor-Mediated Tumor Microenvironment Regulation Using a Sequentially Released Bio-Nanosystem for Enhanced Cancer Photo-Immunotherapy (2022-09-08)
期刊:ACS Applied Materials & Interfaces
影响因子 :10.4
引用产品: NK-92 细胞
-
Natural killer cell-mimicking nanomaterial for overcoming the multidrug resistance of tumor via cascade catalysis (2022-10-14)
期刊:Science China-Materials
影响因子 :8.6
引用产品: NK-92 细胞 , MEMα (不含核苷) 培养基 , β-巯基乙醇 (55mM),1000× , 马血清 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
-
Enhanced Proinflammatory Cytokine Production and Immunometabolic Impairment of NK Cells Exposed to Mycobacterium tuberculosis and Cigarette Smoke (2022-01-05)
期刊:Frontiers in Cellular and Infection Microbiology
影响因子 :5.5
引用产品: NK-92 细胞
-
Presence of TSHR in NK Cells and Action of TSH on NK Cells (2022-01-25)
期刊:NEUROIMMUNOMODULATION
影响因子 :2.8
引用产品: NK-92 细胞
-
PKCι regulates the expression of PDL1 through multiple pathways to modulate immune suppression of pancreatic cancer cells (2021-08-08)
期刊:Cellular Signalling
DOI:10.1016/j.cellsig.2021.110115
影响因子 :4.8
引用产品: NK-92 细胞
-
Transcription factor Sp1 is upregulated by PKCι to drive the expression of YAP1 during pancreatic carcinogenesis (2021-04-17)
期刊:Carcinogenesis
影响因子 :4.7
引用产品: NK-92 细胞
-
CD44 3-Untranslated Region Functions as a Competing Endogenous RNA to Enhance NK Sensitivity of Liver Cancer Stem Cell by Regulating ULBP2 Expression (2019-06-04)
期刊:International Journal of Biological Sciences
影响因子 :9.2
引用产品: NK-92 细胞
FAQs
Q:{{item.question}}
A:
产品资料


识别码示意图