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人结肠黏膜上皮细胞
Human Colonic Mucosal Epithelial Cells
货号：CP-H040
规格：5×10⁵Cells/T25培养瓶
价格： ￥6880
培养基货号： CM-H040
产品概述
人结肠黏膜上皮细胞
Cat NO.: CP-H040
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产品名称：人结肠黏膜上皮细胞
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组织来源：结肠组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人结肠黏膜上皮细胞分离自结肠组织；结肠在右髂窝内续于盲肠，在第3骶椎平面连接直肠。结肠分升结肠、横结肠、降结肠和乙状结肠4部分，大部分固定于腹后壁，结肠的排列酷似英文字母“M”，将小肠包围在内。结肠横切面由内到外依次为：黏膜(上皮层、固有层、黏膜肌层)，黏膜下层(疏松结缔组织)，肌层(内环形、外纵行两层平滑肌)，外膜(纤维膜或浆膜)。肠黏膜上皮细胞是机体内外环境的重要屏障，持续暴露于大量抗原中，也是机体面对病原微生物的第一道防线。因此，肠黏膜上皮细胞除有吸收、分泌和转运等重要生理功能之外，在黏膜先天性和获得性免疫防御机制中也起着重要作用。肠黏膜上皮细胞作为首先接触抗原的细胞，在黏膜免疫反应的起始阶段发挥关键作用，它决定黏膜免疫反应的发生、性质和强度。探讨正常结肠黏膜上皮细胞的分离、体外培养方法，为研究肠黏膜上皮细胞以及与肠黏膜相关疾病建立合适的细胞模型。
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方法简介：
普诺赛实验室分离的人结肠黏膜上皮细胞采用先机械分离法、后胶原酶消化法并通过上皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人结肠黏膜上皮细胞经Cytokeratin-18免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 鼠尾胶原Ⅰ(2-5μg/cm2) 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H040 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 上皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 人结肠黏膜上皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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IRF4 induces M1 macrophage polarization and aggravates ulcerative colitis progression by the Bcl6-dependent STAT3 pathway (2024-01-02)
作者：Jiwei Wang:1.Department of Ultrasound, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China. ; Zhao Wu:1.Nanchang University, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China. ; Yulin Huang:1.Nanchang University, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China. ; Lin Jin:1.Nanchang University, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China. ; Jinyi Xu:1.Nanchang University, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China. ; Zhiyi Yao:1.Nanchang University, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China. ; Xi Ouyang:1.Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China. ; Zhiyong Zhou:1.Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China. ; Shengxun Mao:1.Depar
期刊：IRF4 induces M1 macrophage polarization and aggravates ulcerative colitis progression by the Bcl6-dependent STAT3 pathway
影响因子 ：4.5
引用产品： 人结肠黏膜上皮细胞
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m6A methyltransferase METTL16 mediates immune evasion of colorectal cancer cells via epigenetically regulating PD-L1 expression (2023-08-29)
作者：Ailei Wang:1.Department of Anorectal, Tangshan Traditional Chinese Medicine Hospital, Tangshan, China. ; Yingjie Sun:1.Department of Anorectal, Tangshan Traditional Chinese Medicine Hospital, Tangshan, China. ; Xince Wang:1.Department of Anorectal, Tangshan Traditional Chinese Medicine Hospital, Tangshan, China. ; Zhaofei Yan:1.Department of Anorectal, Tangshan Traditional Chinese Medicine Hospital, Tangshan, China. ; Dongsheng Wang:1.Department of Anorectal, Tangshan Traditional Chinese Medicine Hospital, Tangshan, China. ; Li Zeng:1.Department of Anorectal, Tangshan Traditional Chinese Medicine Hospital, Tangshan, China. ; Qingge Lu:1.Tangshan Traditional Chinese Medicine Hospital, Tangshan, China. ;
期刊：m6A methyltransferase METTL16 mediates immune evasion of colorectal cancer cells via epigenetically regulating PD-L1 expression
影响因子 ：5.2
引用产品： 人结肠黏膜上皮细胞 , RKO 细胞 , HCT 116 细胞 , SW480 [SW-480] 细胞 , COLO 320DM 细胞
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Exosomal circCOL1A2 from cancer cells accelerates colorectal cancer progression via regulating miR-665/LASP1 signal axis (2023-04-12)
作者：Zhi Miao:1.Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China. Electronic address: 1019207167@tju.edu.cn. ; Xiaomeng Zhao:1.Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China. ; Xiang Liu:1.Department of Laboratory Medicine, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430016, China; The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectroni
期刊：Exosomal circCOL1A2 from cancer cells accelerates colorectal cancer progression via regulating miR-665/LASP1 signal axis
DOI：10.1016/j.ejphar.2023.175722
影响因子 ：5.0
引用产品： HT-29 细胞 , HCT 116 细胞 , LoVo 细胞 , SW480 [SW-480] 细胞 , SW620 细胞 , 人结肠黏膜上皮细胞
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Annexin A9 promotes cell proliferation by regulating the Wnt signaling pathway in colorectal cancer (2023-06-22)
作者：Xuemei Lu:1.College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang, China. ; 2.Cancer Institute of Integrated Traditional Chinese and Western Medicine, Key Laboratory of Cancer Prevention and Therapy Combining Traditional Chinese and Western Medicine of Zhejiang Province, Zhejiang Academy of Traditional Chinese Medicine, No. 234, Gucui Road, Hangzhou, 310012, Zhejiang, China. ; Liqiang Hu:1.College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang, China. ; 2.Cancer Institute of Integrated Traditional Chinese and Western Medicine, Key Laboratory of Cancer Prevention and Therapy Combining Traditional Chinese and Western Medicine of Zhejiang Province, Zhejiang Academy of Traditional Chinese Medicine, No. 234, Gucui Road, Hangzhou, 310012, Zhejiang, China. ; Jiayan Mao:1.College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang, China. ; 2.Cancer Institute of
期刊：Annexin A9 promotes cell proliferation by regulating the Wnt signaling pathway in colorectal cancer
DOI：10.1007/s13577-023-00939-x
影响因子 ：4.3
引用产品： LoVo 细胞 , SW480 [SW-480] 细胞 , HCT 116 细胞 , HT-29 细胞 , 人结肠黏膜上皮细胞
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NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA (2023-03-15)
作者：Chuanzhuo Wang:1.Department of Radiology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China. ; He Xin:1.Department of Radiology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China. ; Guangxin Yan:1.Department of Radiology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China. ; Zhaoyu Liu:1.Department of Radiology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China. ;
期刊：NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA
影响因子 ：4.2
引用产品： 人结肠黏膜上皮细胞 , LoVo 细胞 , HCT 116 细胞 , Caco-2 细胞 , Ham's F-12K 培养基 , McCoy's 5A 培养基
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CILP2: A prognostic biomarker associated with immune infiltration in colorectal cancer (2023-04-20)
作者：Xueli Wang:1.College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China. ; Yu Zhang:1.Department of Gastroenterology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China. ; Niping Song:1.The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China. ; Kaiqiang Li:1.Center for Laboratory Medicine, Allergy Center, Department of Transfusion Medicine, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, Zhejiang 310014, China. ; Siyun Lei:1.College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China. ; Jianwei Wang:1.College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China. ; Zhen Wang:1.Center for Laboratory Medicine, Allergy Center, Department of Transfusion Medicine, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzho
期刊：CILP2: A prognostic biomarker associated with immune infiltration in colorectal cancer
DOI：10.1016/j.heliyon.2023.e15535
影响因子 ：4.0
引用产品： HCT 116 细胞 , SW480 [SW-480] 细胞 , SW620 细胞 , RKO 细胞 , 人结肠黏膜上皮细胞
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Potassium voltage‑gated channel subfamily E member 4 facilitates the malignant progression of colon cancer by enhancing EGF containing fibulin extracellular matrix protein 2 expression (2023-07-03)
作者：Yujie Xu:1.Department of Gastrointestinal Surgery, Haikou People's Hospital, Haikou, Hainan 570208, P.R. China. ; Dingmao Wang:1.Department of Gastrointestinal Surgery, Haikou People's Hospital, Haikou, Hainan 570208, P.R. China. ; Guodong Zhao:1.Department of Gastrointestinal Surgery, Haikou People's Hospital, Haikou, Hainan 570208, P.R. China. ;
期刊：Potassium voltage‑gated channel subfamily E member 4 facilitates the malignant progression of colon cancer by enhancing EGF containing fibulin extracellular matrix protein 2 expression
影响因子 ：2.7
引用产品： 人结肠黏膜上皮细胞
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Circ_0000370 Plays an Oncogenic Role in Colorectal Cancer by Regulating the miR-502-5p/SIRT1 Axis (2023-08-10)
作者：Zhu Li:1.Department of Oncology, Peking University Shenzhen Hospital, 1120 Lianhua Road, Futian District, Shenzhen, 518000, Guangdong, China. ; Gangling Tong:1.Department of Oncology, Peking University Shenzhen Hospital, 1120 Lianhua Road, Futian District, Shenzhen, 518000, Guangdong, China. ; Xiaodan Peng:1.Department of Oncology, Peking University Shenzhen Hospital, 1120 Lianhua Road, Futian District, Shenzhen, 518000, Guangdong, China. ; Shubin Wang:1.Department of Oncology, Peking University Shenzhen Hospital, 1120 Lianhua Road, Futian District, Shenzhen, 518000, Guangdong, China. uotvk6@163.com. ;
期刊：Circ_0000370 Plays an Oncogenic Role in Colorectal Cancer by Regulating the miR-502-5p/SIRT1 Axis
DOI：10.1007/s10528-023-10468-9
影响因子 ：2.4
引用产品： HCT 116 细胞 , SW480 [SW-480] 细胞 , SW620 细胞 , 人结肠黏膜上皮细胞
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Circ_0005615 Regulates the Progression of Colorectal Cancer Through the miR-873-5p/FOSL2 Signaling Pathway (2023-03-15)
作者：Lihua Yu:1.Department of Gastroenterology, Affiliated Hospital of Jiangnan University, 1000 Hefeng Road, Wuxi, 214122, Jiangsu, China. ; Feifei Zhang:1.Department of General Surgery, Maternity and Child Health Care of Laizhou, No. 288 Wenhua East Street, Laizhou, 261400, Shandong, People's Republic of China. ; Yeli Wang:1.Department of Anorectal, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, No. 20 Yuhuangding East Road, Yantai, 264000, Shandong, People's Republic of China. vozuhg@163.com. ;
期刊：Circ_0005615 Regulates the Progression of Colorectal Cancer Through the miR-873-5p/FOSL2 Signaling Pathway
DOI：10.1007/s10528-023-10355-3
影响因子 ：2.4
引用产品： HCT 116 细胞 , LoVo 细胞 , SW480 [SW-480] 细胞 , 人结肠黏膜上皮细胞 , RPMI-1640 培养基 , DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100× , 磷酸盐缓冲液 (PBS) 1×
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TRIM29 knockdown prevented the colon cancer progression through decreasing the ubiquitination levels of KRT5 (2023-08-30)
作者：Lihui Sun:1.The Fifth Department of General Surgery, The Third Affiliated Hospital of Jinzhou Medical University, No. 2, Section 5, Heping Road, Jinzhou, Liaoning 121000, China. ; Dawei Wang:1.The Second Department of General Surgery, Dalian Fifth People's Hospital, Dalian, Liaoning 116081, China. ; Zhenyu Chen:1.The Fifth Department of General Surgery, The Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, China. ; Xu Zhu:1.The Fifth Department of General Surgery, The Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, China. ;
期刊：TRIM29 knockdown prevented the colon cancer progression through decreasing the ubiquitination levels of KRT5
影响因子 ：2.2
引用产品： 人结肠黏膜上皮细胞 , SW480 [SW-480] 细胞 , HCT 116 细胞 , SW620 细胞 , LoVo 细胞 , RKO 细胞
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Larotrectinib induces autophagic cell death through AMPK/mTOR signalling in colon cancer (2022-10-17)
作者：Wencheng Kong:1.Zhejiang Province Key Laboratory of Anti-cancer Drug Research, Institute of Pharmacology and Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China. ; 2.Department of Gastroenterological Surgery, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China. ; Hangzhang Zhu:1.Department of Gastroenterological Surgery, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China. ; Sixing Zheng:1.Department of Gastroenterological Surgery, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China. ; Guang Yin:1.Department of Gastroenterological Surgery, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China. ; Panpan Yu:1.Department of Gastroenterological Surgery, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China. ; Yuqian
期刊：Larotrectinib induces autophagic cell death through AMPK/mTOR signalling in colon cancer
影响因子 ：5.3
引用产品： 人结肠黏膜上皮细胞 , 人结肠黏膜上皮细胞完全培养基
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Methyltransferase like 3 enhances pinin mRNA stability through N6-methyladenosine modification to augment tumourigenesis of colon adenocarcinoma (2022-08-22)
作者：Min He:1.Department of Gastroenterology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, P.R. China. ; Danling Jiang:1.Department of Gastroenterology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, P.R. China. ; Anying Xun:1.Department of Gastroenterology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, P.R. China. ; Jian Yang:1.Department of Gastroenterology, Shenzhen Qianhai Shekou Free Trade Zone Hospital, Shenzhen, Guangdong, 518067, P.R. China. ; Qianjiang Luo:1.Department of Gastroenterology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, P.R. China. ; Huihua Wu:1.Department of Gastroenterology, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, P.R. China. ;
期刊：Methyltransferase like 3 enhances pinin mRNA stability through N6-methyladenosine modification to augment tumourigenesis of colon adenocarcinoma
DOI：10.1113/EP090273
影响因子 ：2.9
引用产品： RKO 细胞 , LS 174T [LS174T] 细胞 , CW-2 细胞 , SW1116 细胞 , 人结肠黏膜上皮细胞 , 人结肠黏膜上皮细胞完全培养基
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ISLR affects colon cancer progression by regulating the epithelial–mesenchymal transition signaling pathway (2001-02-20)
作者：Chunhua Chi:1.Department of Anorectal Surgery, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan. ; Tongming Liu:1.Department of Anorectal Surgery, Feicheng People's hospital, Tai An. ; Shengnan Yang:1.Department of Proctology, Changqing District Hospital of Traditional Chinese Medicine, Jinan, Shandong, P.R. China. ; Benjun Wang:1.Department of Anorectal Surgery, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan. ; Weiwei Han:1.Department of Anorectal Surgery, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan. ; Jiansheng Li:1.Department of Anorectal Surgery, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan. ;
期刊：ISLR affects colon cancer progression by regulating the epithelial–mesenchymal transition signaling pathway
DOI：10.1097/CAD.0000000000001233
影响因子 ：2.3
引用产品： 人结肠黏膜上皮细胞 , CX-1 细胞 , SW480 [SW-480] 细胞 , HCT 116 细胞 , HT-29 细胞 , Hs 578T 细胞
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