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产品概述
名称 | RKO (人结肠腺癌细胞) (STR鉴定正确) |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞对温度变化敏感,培养时使用的培养基需预热,温度较低的培养基使用会导致变圆收缩;传代后到细胞完全伸展开需要72h以上。 |
背景描述 | RKO细胞是一个低分化的结肠癌细胞系。RKO细胞含有野生型p53,但缺乏人甲状腺受体核受体(h-TRβ1)。RKO细胞的p53蛋白的水平高于RKO-E6细胞,RKO细胞是RKO-E6细胞和RKO-AS45-1细胞的亲本细胞系。RKO细胞在裸鼠中成瘤,且在软琼脂中形成集落。 |
组织来源 | 结肠癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肠癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~21 hours |
致瘤性 | Yes, in nude mice.Yes, in soft agar. |
受体表达情况 | urokinase receptor (u-PAR) |
保藏机构 | ATCC; CRL-2577 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 8,10 D2S1338 16 D3S1358 16,19 D5S818 11,13 D7S820 8,10 D8S1179 9,13,14 D13S317 8,11 D16S539 12,13 D18S51 11,12 D19S433 14 D21S11 27,30 FGA 20,21,22,23 PentaD 10,11 PentaE 11,13 TH01 6,10 TPOX 11 vWA 16,22 D6S1043 14.1,19 D12S391 15,20 D2S441 11 -
STR鉴定图
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参考文献
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Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis (2024-01-17)
期刊:Acta Pharmaceutica Sinica B
DOI:10.1016/j.apsb.2024.01.006
影响因子 :14.5
引用产品: 特级胎牛血清 , LoVo 细胞 , HT-29 细胞 , HCT 116 细胞 , SW620 细胞 , RKO 细胞 , DLD-1 细胞 , HCT-8 [HRT-18] 细胞
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Enhancing tumor-specific recognition of programmable synthetic bacterial consortium for precision therapy of colorectal cancer (2024-01-20)
期刊:npj Biofilms and Microbiomes
DOI:10.1038/s41522-024-00479-8
影响因子 :9.2
引用产品: CT26.WT 细胞 , RKO 细胞 , SW480 [SW-480] 细胞
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10-Secocycloartane (=9,19-cyclo-9,10-secolanostane) triterpenoid saponins: Huangqiyenins M–X from Astragalus membranaceus (Fisch.) Bge (2024-03-30)
期刊:PHYTOCHEMISTRY
DOI:10.1016/j.phytochem.2024.114072
影响因子 :3.8
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Suppression of Tumor or Host Intrinsic CMTM6 Drives Antitumor Cytotoxicity in a PD-L1–Independent Manner (2023-02-03)
期刊:Cancer Immunology Research
DOI:10.1158/2326-6066.CIR-22-0439
影响因子 :10.1
引用产品: Jurkat, Clone E6-1 细胞 , RKO 细胞 , CT26.WT 细胞 , B16-F10 细胞 , MC38 细胞 , 4T1 细胞 , LLC [LL/2; LLC1] 细胞 , Hepa 1-6 细胞 , YAC-1 细胞 , 293T [HEK-293T] 细胞 , AAV-293 细胞 , STR鉴定
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Attenuated Salmonella-delivered PD-1 siRNA enhances the antitumor effects of EZH2 inhibitors in colorectal cancer (2023-09-12)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2023.110918
影响因子 :5.6
引用产品: RKO 细胞 , HCT 116 细胞
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m6A methyltransferase METTL16 mediates immune evasion of colorectal cancer cells via epigenetically regulating PD-L1 expression (2023-08-29)
期刊:Aging-US
影响因子 :5.2
引用产品: 人结肠黏膜上皮细胞 , RKO 细胞 , HCT 116 细胞 , SW480 [SW-480] 细胞 , COLO 320DM 细胞
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The role of KNTC1 in the regulation of proliferation, migration and tumorigenesis in colorectal cancer (2023-05-23)
期刊:CELLULAR SIGNALLING
DOI:10.1016/j.cellsig.2023.110728
影响因子 :4.8
引用产品: HCT 116 细胞 , RKO 细胞 , SW480 [SW-480] 细胞
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Fluorescent Quinolinium Derivative as Novel Mitochondria Probe and Function Modulator by Targeting Mitochondrial RNA (2023-03-16)
期刊:MOLECULES
影响因子 :4.6
引用产品: RKO 细胞 , 青霉素-链霉素溶液 (双抗),100× , NCM460 细胞
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Screening and validation of the optimal panel of reference genes in colonic epithelium and relative cancer cell lines (2023-10-18)
期刊:Scientific Reports
DOI:10.1038/s41598-023-45174-4
影响因子 :4.6
引用产品: RKO 细胞
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CILP2: A prognostic biomarker associated with immune infiltration in colorectal cancer (2023-04-20)
期刊:Heliyon
DOI:10.1016/j.heliyon.2023.e15535
影响因子 :4.0
引用产品: HCT 116 细胞 , SW480 [SW-480] 细胞 , SW620 细胞 , RKO 细胞 , 人结肠黏膜上皮细胞
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ELAPOR1 suppresses tumor progression in colorectal cancer and indicates favorable prognosis (2023-06-03)
期刊:Cancer Biomarkers
影响因子 :3.1
引用产品: SW620 细胞 , RKO 细胞 , SW480 [SW-480] 细胞 , HT-29 细胞 , Caco-2 细胞 , HCT 116 细胞 , 293T [HEK-293T] 细胞
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TRIM29 knockdown prevented the colon cancer progression through decreasing the ubiquitination levels of KRT5 (2023-08-30)
期刊:Open Life Sciences
影响因子 :2.2
引用产品: 人结肠黏膜上皮细胞 , SW480 [SW-480] 细胞 , HCT 116 细胞 , SW620 细胞 , LoVo 细胞 , RKO 细胞
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Acetyltransferase NAT10 regulates the Wnt/β-catenin signaling pathway to promote colorectal cancer progression via ac4C acetylation of KIF23 mRNA (2022-12-15)
期刊:JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI:10.1186/s13046-022-02551-7
影响因子 :12.7
引用产品: 293T [HEK-293T] 细胞 , NCM460 细胞 , SW480 [SW-480] 细胞 , DLD-1 细胞 , HT-29 细胞 , RKO 细胞 , HCT 116 细胞
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Three New Compounds, Licopyranol A–C, Together with Eighteen Known Compounds Isolated from Glycyrrhiza glabra L. and Their Antitumor Activities (2022-09-23)
期刊:Metabolites
影响因子 :5.6
引用产品: RKO 细胞
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Variation of Saponins in Sanguisorba officinalis L. before and after Processing (Paozhi) and Its Effects on Colon Cancer Cells In Vitro (2022-12-19)
期刊:MOLECULES
影响因子 :4.9
引用产品: HCT 116 细胞 , RKO 细胞
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EGCG Enhances the Chemosensitivity of Colorectal Cancer to Irinotecan through GRP78-MediatedEndoplasmic Reticulum Stress (2022-09-13)
期刊:Journal of Oncology
影响因子 :4.5
引用产品: RKO 细胞
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Methyltransferase like 3 enhances pinin mRNA stability through N6-methyladenosine modification to augment tumourigenesis of colon adenocarcinoma (2022-08-22)
期刊:EXPERIMENTAL PHYSIOLOGY
DOI:10.1113/EP090273
影响因子 :2.9
引用产品: RKO 细胞 , LS 174T [LS174T] 细胞 , CW-2 细胞 , SW1116 细胞 , 人结肠黏膜上皮细胞 , 人结肠黏膜上皮细胞完全培养基
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miR‑146a‑5p negatively regulates the IL‑1β‑stimulated inflammatory response via downregulation of the IRAK1/TRAF6 signaling pathway in human intestinal epithelial cells (2022-08-05)
期刊:Experimental and Therapeutic Medicine
影响因子 :2.8
引用产品: RKO 细胞
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PD-1-siRNA Delivered by Attenuated Salmonella Enhances the Antitumor Effect of Chloroquine in Colon Cancer (2007-01-20)
期刊:Frontiers in Immunology
影响因子 :7.3
引用产品: HCT 116 细胞 , RKO 细胞
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Notum palmitoleoyl-protein carboxylesterase regulates Fas cell surface death receptor-mediated apoptosis via the Wnt signaling pathway in colon adenocarcinoma (2008-01-20)
期刊:Bioengineered
DOI:10.1080/21655979.2021.1961657
影响因子 :4.9
引用产品: RKO 细胞 , HCT 116 细胞 , SW620 细胞
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Lycorine Induces autophagy-associated apoptosis by targeting MEK2 and enhances vemurafenib activity in colorectal cancer (2001-00-20)
期刊:Aging-US
影响因子 :5.2
引用产品: HCT 116 细胞 , SW480 [SW-480] 细胞 , RKO 细胞 , CT26.WT 细胞
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The bromodomain and extra-terminal domain inhibitor JQ1 synergistically sensitizes human colorectal cancer cells to topoisomerase I inhibitors through repression of Mre11-mediated DNA repair pathway (2009-00-20)
期刊:Investigational New Drugs
DOI:10.1007/s10637-020-01014-0
影响因子 :3.4
引用产品: HCT 116 细胞 , SW480 [SW-480] 细胞 , SW620 细胞 , HT-29 细胞 , LoVo 细胞 , RKO 细胞
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Pseudophosphatase STYX promotes tumor growth and metastasis by inhibiting FBXW7 function in colorectal cancer (2006-09-20)
期刊:Cancer Letters
DOI:10.1016/j.canlet.2019.04.014
影响因子 :9.7
引用产品: SW480 [SW-480] 细胞 , SW620 细胞 , HCT 116 细胞 , HT-29 细胞 , RKO 细胞 , LoVo 细胞
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