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产品概述
名称 | SW620 (人结肠癌细胞) (STR鉴定正确) |
别称 | SW-620; SW 620; SW.620 |
种属 | 人类 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,100%, 温度:37℃
|
培养方案B(可选) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
注意事项 | 1.该细胞推荐使用Leibovitz's L-15培养基进行培养,Leibovitz's L-15不可以通入二氧化碳,会产生细胞毒性;2.如您没有无二氧化碳的培养箱,可使用DMEM替代Leibovitz's L-15,使用DMEM培养基时即可正常通入5%二氧化碳;3.配套专用培养基默认Leibovitz's L-15配置,如需DMEM配方,请联系销售下单备注更改。 |
背景描述 | SW620细胞是从一个51岁男性白人组织中分离得到的,由A·Leibovitz等从一个淋巴结建株。SW620细胞主要由无绒毛的小园球细胞和双极细胞组成;SW620细胞仅合成少量癌胚抗原(CEA),在裸鼠中有高度的致瘤性。 |
年龄(性别) | 男性;51岁 |
组织来源 | 结直肠腺癌,来自转移淋巴结 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肠癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~20-26 hours |
致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
抗原表达情况 | Blood Type A; Rh+ |
基因表达情况 | Carcinoembryonic antigen (CEA) 0.15 ng/10^6 cells/10 days; transforming growth factor alpha; matrilysin. The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative. The cells are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. |
保藏机构 | ATCC; CCL-227 ECACC; 87051203 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 13,14 D2S1338 17,24 D3S1358 16 D5S818 13 D7S820 8,9 D8S1179 13 D13S317 12 D16S539 9,13 D18S51 13 D19S433 13 D21S11 30,30.2 FGA 24 PentaD 9,15 PentaE 10 TH01 8 TPOX 11 vWA 16 D6S1043 11,12 D12S391 17 D2S441 10,15 -
STR鉴定图
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参考文献
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Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis (2024-01-17)
期刊:Acta Pharmaceutica Sinica B
DOI:10.1016/j.apsb.2024.01.006
影响因子 :14.5
引用产品: 特级胎牛血清 , LoVo 细胞 , HT-29 细胞 , HCT 116 细胞 , SW620 细胞 , RKO 细胞 , DLD-1 细胞 , HCT-8 [HRT-18] 细胞
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Biotin-decorated celastrol-loaded ZIF-8 nanoparticles induce ferroptosis for colorectal cancer therapy (2024-02-29)
期刊:MATERIALS & DESIGN
DOI:10.1016/j.matdes.2024.112814
影响因子 :8.4
引用产品: HCT 116 细胞 , SW620 细胞
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Colorectal cancer with low SLC35A3 is associated with immune infiltrates and poor prognosis (2024-01-03)
期刊:Scientific Reports
DOI:10.1038/s41598-023-51028-w
影响因子 :4.6
引用产品: NCM460细胞 , HCT 116 细胞 , HT-29 细胞 , SW620 细胞
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MiR-214-3p overexpression-triggered chondroitin polymerizing factor (CHPF) inhibition modulates the ferroptosis and metabolism in colon cancer (2024-01-08)
期刊:KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
影响因子 :3.3
引用产品: SW480 [SW-480] 细胞 , SW620 细胞 , LoVo 细胞 , DMEM高糖 培养基 , 青霉素-链霉素溶液 (双抗),100× , Ham's F-12K 培养基
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Forsythiaside A alleviates acute lung injury by inhibiting inflammation and epithelial barrier damages in lung and colon through PPAR-γ/RXR-α complex (2023-08-12)
期刊:Journal of Advanced Research
DOI:10.1016/j.jare.2023.08.006
影响因子 :10.7
引用产品: Ham's F-12K 培养基 , 特级胎牛血清 , A549 [A-549] 细胞 , SW620 细胞
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Mollugin suppresses proliferation and drives ferroptosis of colorectal cancer cells through inhibition of insulin-like growth factor 2 mRNA binding protein 3/glutathione peroxidase 4 axis (2023-09-05)
期刊:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2023.115427
影响因子 :7.5
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Mechanism of Bazhen decoction in the treatment of colorectal cancer based on network pharmacology, molecular docking, and experimental validation (2023-09-20)
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2023.1235575
影响因子 :7.3
引用产品: HCT 116 细胞 , SW620 细胞 , MC38 细胞
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Identification and validation of a novel prognosis model based on m5C-related long non-coding RNAs in colorectal cancer (2023-09-05)
期刊:Cancer Cell International
DOI:10.1186/s12935-023-03025-2
影响因子 :5.8
引用产品: NCM460 细胞 , SW620 细胞 , HCT 116 细胞 , SW480 [SW-480] 细胞 , DLD-1 细胞 , LoVo 细胞 , HT-29 细胞
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The combination of phosphoinositol with inositol suppresses colorectal cancer growth and metastasis by regulating CLDN23/ RhoA /ROCK1 pathway (2023-11-08)
期刊:Journal of Functional Foods
影响因子 :5.6
引用产品: SW620 细胞 , SW480 [SW-480] 细胞
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Palmitic acid-induced ferroptosis via CD36 activates ER stress to break calcium-iron balance in colon cancer cells (2023-03-12)
期刊:FEBS Journal
影响因子 :5.4
引用产品: HT-29 细胞 , HCT 116 细胞 , SW480 [SW-480] 细胞 , SW620 细胞 , NCM460 细胞
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Exosomal circCOL1A2 from cancer cells accelerates colorectal cancer progression via regulating miR-665/LASP1 signal axis (2023-04-12)
期刊:EUROPEAN JOURNAL OF PHARMACOLOGY
DOI:10.1016/j.ejphar.2023.175722
影响因子 :5.0
引用产品: HT-29 细胞 , HCT 116 细胞 , LoVo 细胞 , SW480 [SW-480] 细胞 , SW620 细胞 , 人结肠黏膜上皮细胞
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Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway (2023-09-15)
期刊:MOLECULES
影响因子 :4.6
引用产品: SW620 细胞 , SW480 [SW-480] 细胞 , A549 [A-549] 细胞 , MGC80-3 [MGC-803] 细胞
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The Upregulation of GSTO2 is Associated with Colon Cancer Progression and a Poor Prognosis (2023-01-11)
期刊:Journal of Oncology
影响因子 :4.5
引用产品: HT-29 细胞 , HCT 116 细胞 , SW480 [SW-480] 细胞 , SW620 细胞
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CILP2: A prognostic biomarker associated with immune infiltration in colorectal cancer (2023-04-20)
期刊:Heliyon
DOI:10.1016/j.heliyon.2023.e15535
影响因子 :4.0
引用产品: HCT 116 细胞 , SW480 [SW-480] 细胞 , SW620 细胞 , RKO 细胞 , 人结肠黏膜上皮细胞
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P300 increases CSNK2A1 expression which accelerates colorectal cancer progression through activation of the PI3K-AKT-mTOR axis (2023-06-28)
期刊:EXPERIMENTAL CELL RESEARCH
DOI:10.1016/j.yexcr.2023.113694
影响因子 :3.7
引用产品: HCT 116 细胞 , SW480 [SW-480] 细胞 , SW620 细胞 , LoVo 细胞 , HT-29 细胞
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DEPDC1B is involved in the proliferation, metastasis, cell cycle arrest and apoptosis of colon cancer cells by regulating NUP37 (2023-05-16)
期刊:Molecular Medicine Reports
影响因子 :3.4
引用产品: SW620 细胞 , HCT-8 [HRT-18] 细胞 , HCT 116 细胞
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ELAPOR1 suppresses tumor progression in colorectal cancer and indicates favorable prognosis (2023-06-03)
期刊:Cancer Biomarkers
影响因子 :3.1
引用产品: SW620 细胞 , RKO 细胞 , SW480 [SW-480] 细胞 , HT-29 细胞 , Caco-2 细胞 , HCT 116 细胞 , 293T [HEK-293T] 细胞
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Maintenance of the Expression of c-FLIPL by Hsp70 to Resist Licochalcone A-Induced Anti-Colorectal Cancer Effect through ERK-Mediated Autophagy Induction (2023-12-01)
期刊:Frontiers in Bioscience-Landmark
影响因子 :3.1
引用产品: SW620 细胞 , HCT 116 细胞
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Circ_0087862 promotes tumorigenesis and glycolysis in colorectal cancer by sponging miR-296-3p to regulate PGK1 expression (2023-07-17)
期刊:PATHOLOGY RESEARCH AND PRACTICE
影响因子 :2.8
引用产品: LoVo 细胞 , SW620 细胞 , HT-29 细胞 , SW480 [SW-480] 细胞
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Development and validation of cuproptosis-related lncRNA signatures for prognosis prediction in colorectal cancer (2023-03-22)
期刊:BMC Medical Genomics
DOI:10.1186/s12920-023-01487-x
影响因子 :2.7
引用产品: FHC 细胞 , SW480 [SW-480] 细胞 , SW620 细胞 , HCT-8 [HRT-18] 细胞 , HT-29 细胞 , LoVo 细胞
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Circ_0000370 Plays an Oncogenic Role in Colorectal Cancer by Regulating the miR-502-5p/SIRT1 Axis (2023-08-10)
期刊:BIOCHEMICAL GENETICS
DOI:10.1007/s10528-023-10468-9
影响因子 :2.4
引用产品: HCT 116 细胞 , SW480 [SW-480] 细胞 , SW620 细胞 , 人结肠黏膜上皮细胞
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Circ_0005785 Silencing Constrains the Functional Properties of Colorectal Cancer Cells Depending on miR-7-5p/DNMT3A Axis (2023-09-20)
期刊:BIOCHEMICAL GENETICS
DOI:10.1007/s10528-023-10522-6
影响因子 :2.4
引用产品: LoVo 细胞 , HCT 116 细胞 , SW620 细胞
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Auranofin inhibits the occurrence of colorectal cancer by promoting mTOR-dependent autophagy and inhibiting epithelial-mesenchymal transformation (2023-08-25)
期刊:ANTI-CANCER DRUGS
DOI:10.1097/CAD.0000000000001540
影响因子 :2.3
引用产品: HCT 116 细胞 , SW620 细胞
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TRIM29 knockdown prevented the colon cancer progression through decreasing the ubiquitination levels of KRT5 (2023-08-30)
期刊:Open Life Sciences
影响因子 :2.2
引用产品: 人结肠黏膜上皮细胞 , SW480 [SW-480] 细胞 , HCT 116 细胞 , SW620 细胞 , LoVo 细胞 , RKO 细胞
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Hsa_Circ_0104206 is An Oncogenic circRNA in Colon Cancer by Targeting Mir-188–3p/CCNA2 Axis (2023-06-05)
期刊:HORMONE AND METABOLIC RESEARCH
影响因子 :2.2
引用产品: HCT-8 [HRT-18] 细胞 , SW620 细胞 , HCT 116 细胞 , SW480 [SW-480] 细胞 , DMEM高糖 培养基 , RPMI-1640 培养基 , 特级胎牛血清
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The combination of inositol hexaphosphate and inositol inhibits metastasis of colorectal cancer cells by upregulating claudin 7 (2023-06-08)
期刊:BIOLOGICAL & PHARMACEUTICAL BULLETIN
影响因子 :2.0
引用产品: SW480 [SW-480] 细胞 , SW620 细胞
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Cryptotanshinone, a Potential SIRT3 Inhibitor, Suppresses Colorectal Cancer Proliferation in vitro and in vivo (2023-08-18)
期刊:Natural Product Communications
影响因子 :1.8
引用产品: SW480 [SW-480] 细胞 , SW620 细胞
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Metabolism-Based Molecular Subtyping Endows Effective Ketogenic Therapy in p53-Mutant Colon Cancer (2022-08-28)
期刊:Advanced Science
影响因子 :17.5
引用产品: SW480 [SW-480] 细胞 , HT-29 细胞 , SW620 细胞
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Circ_0011385 knockdown inhibits cell proliferation, migration and invasion, whereas promotes cell apoptosis by regulating miR-330-3p/MYO6 axis in colorectal cancer (2001-02-20)
期刊:Biomedical Journal
影响因子 :5.5
引用产品: SW480 [SW-480] 细胞 , SW620 细胞 , LoVo 细胞 , HCT 116 细胞 , RPMI-1640 培养基 , DMEM高糖 培养基 , 特级胎牛血清
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Circ_0056618 enhances PRRG4 expression by competitively binding to miR-411-5p to promote the malignant progression of colorectal cancer (2022-08-02)
期刊:MOLECULAR AND CELLULAR BIOCHEMISTRY
DOI:10.1007/s11010-022-04525-x
影响因子 :3.8
引用产品: LoVo 细胞 , SW620 细胞 , SW480 [SW-480] 细胞 , HCT 116 细胞 , 特级胎牛血清 , Leibovitz's L-15 培养基 , McCoy's 5A 培养基
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Identification and characterization of the inhibitory effect of ART on colorectal cancer cells using Raman spectroscopy (2001-02-20)
期刊:Journal of Raman Spectroscopy
DOI:10.1002/jrs.6292
影响因子 :2.5
引用产品: SW480 [SW-480] 细胞 , SW620 细胞 , HCT 116 细胞 , Leibovitz's L-15 培养基 , McCoy's 5A 培养基
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High Expression of circ_0001821 Promoted Colorectal Cancer Progression Through miR-600/ISOC1 Axis (2022-08-09)
期刊:BIOCHEMICAL GENETICS
DOI:10.1007/s10528-022-10262-z
影响因子 :2.2
引用产品: SW620 细胞 , HCT 116 细胞
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Raltitrexed as a synergistic hyperthermia chemotherapy drug screened in patient-derived colorectal cancer organoids (2008-01-20)
期刊:Cancer Biology & Medicine
DOI:10.20892/j.issn.2095-3941.2020.0566
影响因子 :5.5
引用产品: SW620 细胞 , SW480 [SW-480] 细胞 , DLD-1 细胞 , COLO 205 细胞
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Long non-coding RNA HOXA-AS3 facilitates the malignancy in colorectal cancer by miR-4319/SPNS2 axis (2010-01-20)
期刊:Journal Of Physiology And Biochemistry
DOI:10.1007/s13105-021-00832-x
影响因子 :5.1
引用产品: SW480 [SW-480] 细胞 , SW620 细胞 , HCT 116 细胞 , COLO 205 细胞 , LoVo 细胞
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Notum palmitoleoyl-protein carboxylesterase regulates Fas cell surface death receptor-mediated apoptosis via the Wnt signaling pathway in colon adenocarcinoma (2008-01-20)
期刊:Bioengineered
DOI:10.1080/21655979.2021.1961657
影响因子 :4.9
引用产品: RKO 细胞 , HCT 116 细胞 , SW620 细胞
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MicroRNA‑129 inhibits colorectal cancer cell proliferation, invasion and epithelial‑to‑mesenchymal transition by targeting SOX4 (2003-01-20)
期刊:Oncology Reports
影响因子 :4.2
引用产品: SW620 细胞 , SW480 [SW-480] 细胞
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Sevoflurane inhibits malignant progression of colorectal cancer via hsa_circ_0000231-mediated miR-622 (2006-01-20)
期刊:Journal of Biological Research-Thessaloniki
DOI:10.1186/s40709-021-00145-6
影响因子 :3.3
引用产品: SW620 细胞 , HCT 116 细胞 , 磷酸盐缓冲液 (PBS) 1× , RPMI-1640完全培养基 (含10%FBS) , SW620细胞专用培养基 (L15)
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NDRG4 sensitizes CRC cells to 5-FU by upregulating DDIT3 expression (2009-01-20)
期刊:Oncology Letters
影响因子 :2.9
引用产品: SW480 [SW-480] 细胞 , SW620 细胞 , 293T [HEK-293T] 细胞
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microRNA-4429-5p suppresses the malignant development of colon cancer by targeting matrix metalloproteinase 16 (2008-01-20)
期刊:In Vitro Cellular & Developmental Biology-Animal
DOI:10.1007/s11626-021-00603-4
影响因子 :2.7
引用产品: SW480 [SW-480] 细胞 , SW620 细胞 , HCT 116 细胞 , LoVo 细胞 , Caco-2 细胞 , Leibovitz's L-15 培养基 , Ham's F-12K 培养基 , McCoy's 5A 培养基
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Down-regulation of lncRNA DNAJC3-AS1 inhibits colon cancer via regulating miR-214-3p/LIVIN axis (2004-00-20)
期刊:Bioengineered
DOI:10.1080/21655979.2020.1757224
影响因子 :4.9
引用产品: SW620 细胞 , SW480 [SW-480] 细胞 , Caco-2 细胞 , LoVo 细胞 , 293T [HEK-293T] 细胞 , Ham's F-12K 培养基
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miR-370-3p Alleviates Ulcerative Colitis-Related Colorectal Cancer in Mice Through Inhibiting the Inflammatory Response and Epithelial-Mesenchymal Transition (2003-00-20)
期刊:Drug Design Development and Therapy
影响因子 :4.8
引用产品: HCT 116 细胞 , SW620 细胞 , HT-29 细胞 , McCoy's 5A 培养基
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The bromodomain and extra-terminal domain inhibitor JQ1 synergistically sensitizes human colorectal cancer cells to topoisomerase I inhibitors through repression of Mre11-mediated DNA repair pathway (2009-00-20)
期刊:Investigational New Drugs
DOI:10.1007/s10637-020-01014-0
影响因子 :3.4
引用产品: HCT 116 细胞 , SW480 [SW-480] 细胞 , SW620 细胞 , HT-29 细胞 , LoVo 细胞 , RKO 细胞
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CircCSNK1G1 Contributes to the Development of Colorectal Cancer by Increasing the Expression of MYO6 via Competitively Targeting miR-455-3p (2010-00-20)
期刊:Cancer Management and Research
影响因子 :3.3
引用产品: HCT 116 细胞 , SW620 细胞 , HCT 116细胞专用培养基 , SW620细胞专用培养基 (L15)
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Bone Morphogenetic Protein 1 Targeting COL1A1 and COL1A2 to Regulate the Epithelial-Mesenchymal Transition Process of Colon Cancer SW620 Cells (2003-00-20)
期刊:Journal of Nanoscience and Nanotechnology
影响因子 :0.0
引用产品: SW620 细胞
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Pseudophosphatase STYX promotes tumor growth and metastasis by inhibiting FBXW7 function in colorectal cancer (2006-09-20)
期刊:Cancer Letters
DOI:10.1016/j.canlet.2019.04.014
影响因子 :9.7
引用产品: SW480 [SW-480] 细胞 , SW620 细胞 , HCT 116 细胞 , HT-29 细胞 , RKO 细胞 , LoVo 细胞
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Structural elucidation of an acidic polysaccharide from Citrus grandis ‘Tomentosa’ and its anti-proliferative effects on LOVO and SW620 cells (2010-09-20)
期刊:International Journal of Biological Macromolecules
DOI:10.1016/j.ijbiomac.2019.07.117
影响因子 :8.2
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A 3-circular RNA signature as a noninvasive biomarker for diagnosis of colorectal cancer (2011-09-20)
期刊:Cancer Cell International
影响因子 :5.8
引用产品: HCT 116 细胞 , SW480 [SW-480] 细胞 , SW620 细胞
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SPNS2 promotes the malignancy of colorectal cancer cells via regulating Akt and ERK pathway (2006-09-20)
期刊:Clinical And Experimental Pharmacology And Physiology
影响因子 :2.9
引用产品: SW480 [SW-480] 细胞 , SW620 细胞 , HCT 116 细胞 , COLO 205 细胞 , LoVo 细胞
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miRNA-101通过靶向CDK8调控结肠癌细胞侵袭和Wnt/β-catenin通路活化 (2005-08-20)
期刊:肿瘤防治研究
DOI:10.3971/j.issn.1000-8578.2018.17.1558
影响因子 :0.7
引用产品: HCT 116 细胞 , HT-29 细胞 , LoVo 细胞 , SW620 细胞 , COLO 205 细胞
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