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产品概述
胰蛋白酶（Trypsin）是一种丝氨酸水解酶，它能把多肽链中赖氨酸和精氨酸残基中的羧基侧切段，水解细胞间的蛋白质，破坏细胞间的连接，从而使组织或贴壁细胞离散成单个细胞。胰酶分散细胞的活性与组织或细胞的特性、胰酶浓度、温度和作用时间有关，在pH 8.0和37℃时，胰酶的作用能力最强，因此使用胰酶时，应把握好浓度、温度和时间，以免消化过度造成细胞损伤。
一般常用胰酶的工作浓度为0.25%，而半贴壁细胞或对胰酶敏感的细胞常采用低浓度（0.05%）的胰酶进行细胞消化。由于EDTA能够螯合Ca2+和Mg2+，从而破坏细胞连接促进细胞的解离，因此在胰酶溶液中常常会加入一定量的EDTA混合使用，以增强解离效果。
产品信息
形态 | 液体 |
规格 | 100mL |
pH | 7.2-7.4 |
溶剂 | D-Hank's液 |
胰酶蛋白酶浓度 | 0.5 g/L |
EDTA•2Na | 0.2 g/L |
酚红指示剂 | 无 |
储存条件 | -5~-20℃ |
运输条件 | 低温 |
有效期 | 12个月 |
注意事项
1、由于不同的组织或者细胞对胰酶的作用反应不一样，因此操作人员应根据实际情况，确定最佳消化时间；消化细胞时间不宜过长，否则会影响细胞贴壁和生长状况；
2、使用本产品时应注意无菌操作，避免污染；
3、不宜长时间放置于室温环境或2-8℃长期保存；
4、2-8℃中解冻，摇匀后使用，切忌反复冻融，用量较少时建议分装冻存。
5、由于D-Hank's平衡盐溶液NaHCO3含量较低（0.35g/L），因此该型胰蛋白酶溶液不能用于5% CO2的环境，若放入CO2培养箱，溶液将迅速变酸，使用时应注意；
6、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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Self-immolative nanocapsules precisely regulate depressive neuronal microenvironment for synergistic antidepression therapy (2023-08-17)
作者：Ziyao Liu:1.Department of Nuclear Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China. ; 2.Key Laboratory of Biological, Nanotechnology of National Health Commission, Xiangya Hospital, Central South University, Changsha, 410008, China. ; Bei Chen:1.Department of Nuclear Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China. ; Shijun Xiang:1.Department of Nuclear Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China. ; Shuo Hu:1.Department of Nuclear Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China. hushuo2018@163.com. ; 2.National Clinical Research Center for Geriatric Diseases, Xiangya Hospital, Central South University, Changsha, 410008, China. hushuo2018@163.com. ; 3.Key Laboratory of Biological, Nanotechnology of National Health Commission, Xiangya Hospital, Central South University, Changsha, 410008, China. hushuo2018@163.com. ;
期刊：Self-immolative nanocapsules precisely regulate depressive neuronal microenvironment for synergistic antidepression therapy
DOI：10.1186/s12951-023-02008-9
影响因子 ：10.2
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Injectable Hydrogel Containing TiO Nanosheets for Synergistic Photothermal/Thermodynamic Therapy (2023-07-07)
作者：Jianghao Xing:1.Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, P. R. China. ; 2.School of Biomedical Engineering, Research and Engineering Center of Biomedical Materials, Anhui Medical University, Hefei 230032, P. R. China. ; Yuqi Yang:1.Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou 215123, China. ; Wei Zhang:1.School of Biomedical Engineering, Research and Engineering Center of Biomedical Materials, Anhui Medical University, Hefei 230032, P. R. China. ; Jianqin Yan:1.Department of Pharmaceutics, School of Pharmacy, Qingdao University, Qingdao 266021, China. ; Haisheng Qian:1.School of Biomedical Engineering, Research and Engineering Center of Biomedical Materials, Anhui Medical University, Hefei 230032, P. R. China. ; Jiqing Hao:1.Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei 230
期刊：Injectable Hydrogel Containing TiO Nanosheets for Synergistic Photothermal/Thermodynamic Therapy
影响因子 ：9.5
引用产品： 青霉素-链霉素溶液 (双抗)，100× , RPMI-1640 培养基 , 特级胎牛血清 , 0.05%胰蛋白酶溶液 (溶于PBS) , 磷酸盐缓冲液 (PBS) 1×
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Silver Carp (Hypophthalmichthys molitrix) Scale Collagen Peptides-1 (SCPs1) Inhibit Melanogenesis through Downregulation of the cAMP-CREB Signaling Pathway (2023-05-24)
作者：Hai-Lan Li:1.Key Laboratory of Cold Chain Logistics Technology for Agro-Product, Ministry of Agriculture and Rural Affairs, Institute of Agro-Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China. ; Mei-Jin Li:1.Key Laboratory of Cold Chain Logistics Technology for Agro-Product, Ministry of Agriculture and Rural Affairs, Institute of Agro-Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China. ; 2.Key Laboratory of Fermentation Engineering, Ministry of Education, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, China. ; Guang-Quan Xiong:1.Key Laboratory of Cold Chain Logistics Technology for Agro-Product, Ministry of Agriculture and Rural Affairs, Institute of Agro-Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agri
期刊：Silver Carp (Hypophthalmichthys molitrix) Scale Collagen Peptides-1 (SCPs1) Inhibit Melanogenesis through Downregulation of the cAMP-CREB Signaling Pathway
影响因子 ：5.9
引用产品： 特级胎牛血清 , 0.25%胰蛋白酶溶液 (含EDTA，溶于PBS)
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Mesenchymal Stem Cells Ameliorate DSS-Induced Experimental Colitis by Modulating the Gut Microbiota and MUC-1 Pathway (2023-05-10)
作者：Han Wang:1.School of Basic Medicine, Qingdao University, Qingdao, 266071, People's Republic of China. ; 2.Laboratory of Molecular Diagnosis and Regenerative Medicine, The Affiliated Hospital of Qingdao University, Qingdao, 266000, People's Republic of China. ; Yang Sun:1.School of Basic Medicine, Qingdao University, Qingdao, 266071, People's Republic of China. ; 2.Laboratory of Molecular Diagnosis and Regenerative Medicine, The Affiliated Hospital of Qingdao University, Qingdao, 266000, People's Republic of China. ; Feng-Jun Xiao:1.Beijing Institute of Radiation Medicine, Beijing, 100850, People's Republic of China. ; Xia Zhao:1.Laboratory of Molecular Diagnosis and Regenerative Medicine, The Affiliated Hospital of Qingdao University, Qingdao, 266000, People's Republic of China. ; 2.Department of Hematology, The Affiliated Hospital of Qingdao University, Qingdao, 266000, People's Republic of China. ; Wei-Yuan Zhang:1.School of Basic Medicine, Qingdao University, Qingdao, 266071, Pe
期刊：Mesenchymal Stem Cells Ameliorate DSS-Induced Experimental Colitis by Modulating the Gut Microbiota and MUC-1 Pathway
影响因子 ：4.5
引用产品： 0.05%胰蛋白酶溶液 (溶于PBS)
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Insights into the characteristics of primary radioresistant cervical cancer using single-cell transcriptomics (2023-03-03)
作者：Biyuan Xing:1.Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; 2.Institute of Radiation Oncology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; Congli Pu:1.Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; 2.Institute of Radiation Oncology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; Yunshang Chen:1.Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; 2.Institute of Radiation Oncology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; Yuhan Sheng:1.Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ;
期刊：Insights into the characteristics of primary radioresistant cervical cancer using single-cell transcriptomics
DOI：10.1007/s13577-023-00882-x
影响因子 ：4.3
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