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产品概述
| 名称 | PLC/PRF/5 (人肝癌亚力山大细胞) (STR鉴定正确) |
| 别称 | PLC-PRF-5; PLC PRF 5; PLC/PRF5; PLCPRF5; PLC-8024; PLC8024; PLC; Alexander cells; Alexander; Primary Liver Carcinoma/Poliomyelitis Research Foundation/5 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
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| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | PLC/PRF/5细胞分泌乙肝病毒表面抗原(HBsAg)；PLC/PRF/5细胞原先被支原体污染，后用BM-cycline去除支原体。 |
| 组织来源 | 肝癌；亚力山大细胞 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肝胆癌细胞 |
| 生物安全等级 | BSL-2 [Cells contain hepatitis B] |
| 倍增时间 | ~36-48 hours |
| 基因表达情况 | hepatitis virus B surface antigen (HBsAg) |
| 保藏机构 | ATCC; CRL-8024 ECACC; 85061113 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10 D2S1338 19,22 D3S1358 15 D5S818 12 D7S820 9,11 D8S1179 13,16 D13S317 11,12 D16S539 13 D18S51 17 D19S433 11,13 D21S11 30,33.2 FGA 19.2,25 PentaD 6,10 PentaE 10,16 TH01 8 TPOX 8 vWA 15,16 D6S1043 13,21 D12S391 15,20 D2S441 12,14 -
STR鉴定图
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参考文献
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RBBP7, regulated by SP1, enhances the Warburg effect to facilitate the proliferation of hepatocellular carcinoma cells via PI3K/AKT signaling (2024-02-18)
作者：Yuan Fang:1.Organ Transplantation Center, The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming, 650032, Yunnan, China. ; WeiQiang Tang:1.Institute of Clinical Medicine, Hengyang Medical School, The First Affiliated Hospital, University of South China, Hengyang, Hunan, People's Republic of China. ; Siming Qu:1.Organ Transplantation Center, The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming, 650032, Yunnan, China. ; ZhiTao Li:1.Organ Transplantation Center, The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming, 650032, Yunnan, China. ; XiaoLi Zhang:1.Gastrointestinal and Hernia Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, People's Republic of China. ; YingLei Miao:1.Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Yunnan, People's Republic of China. ; 2.Yunnan Province Clinical Research Center for
期刊：RBBP7, regulated by SP1, enhances the Warburg effect to facilitate the proliferation of hepatocellular carcinoma cells via PI3K/AKT signaling
DOI：10.1186/s12967-024-04964-8
影响因子 ：7.4
引用产品： Hep 3B2.1-7 细胞 , HuH-7 细胞 , PLC/PRF/5 细胞 , Li-7 细胞 , THLE-2 细胞
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Combining a glycolysis‑related prognostic model based on scRNA‑Seq with experimental verification identifies ZFP41 as a potential prognostic biomarker for HCC (2024-03-19)
作者：Yu Teng:1.West China School of Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China. ; Jianrong Xu:1.Research Center for Biliary Diseases, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China. ; Yaoqun Wang:1.Research Center for Biliary Diseases, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China. ; Ningyuan Wen:1.Research Center for Biliary Diseases, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China. ; Hui Ye:1.Research Center for Biliary Diseases, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China. ; Bei Li:1.Research Center for Biliary Diseases, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China. ;
期刊：Combining a glycolysis‑related prognostic model based on scRNA‑Seq with experimental verification identifies ZFP41 as a potential prognostic biomarker for HCC
影响因子 ：3.4
引用产品： 293T [HEK-293T] 细胞 , HuH-7 细胞 , PLC/PRF/5 细胞
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The diagnostic, prognostic, drug sensitivity and ceRNA internet of PPP4R1 in liver hepatocellular carcinoma (LIHC) (2024-02-26)
作者：Xiaowei Li:1.Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou, China. ; Yinglian Pan:1.Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou, China. ; 2.Department of Medical Oncology, First Affiliated Hospital, Hainan Medical College, Haikou, China. ; Siren Feng:1.Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou, China. ; Weimei Xing:1.Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou, China. ; Bo Lin:1.Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou, China. ; Wei Li:1.Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou, China. ; Mengsen Li:1.Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, Hainan Medical College, Haikou, China. ; 2.Department of Medical Oncology, Se
期刊：The diagnostic, prognostic, drug sensitivity and ceRNA internet of PPP4R1 in liver hepatocellular carcinoma (LIHC)
影响因子 ：0.9
引用产品： LX-2 细胞 , HLE 细胞 , HuH-7 细胞 , PLC/PRF/5 细胞 , Hep G2 细胞 , 特级胎牛血清
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Conjugating 4β-NH-(5-Aminoindazole)-podophyllotoxin and Galectin-1-Targeted Aptamer for Synergistic Chemo-Immunotherapy of Hepatocellular Carcinoma (2023-05-04)
作者：Ying Cong:1.State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China. ; Shu-Yue Zhang:1.China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, China. ; Paula Yun-Zhi Tang:1.Jinan Zhensheng School, Jinan, 250102, China. ; Hong-Mei Li:1.State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China. ; Xue Liu:1.Jinan Food and Drug Inspection and Testing Center, Jinan, 250101, China. ; Wei Zhao:1.State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China. ; Ya-Jie Tang:1.State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China. ;
期刊：Conjugating 4β-NH-(5-Aminoindazole)-podophyllotoxin and Galectin-1-Targeted Aptamer for Synergistic Chemo-Immunotherapy of Hepatocellular Carcinoma
影响因子 ：10.0
引用产品： Hep G2 细胞 , HuH-7 细胞 , PLC/PRF/5 细胞 , SNU387 细胞 , MCF7 [MCF-7] 细胞 , Hepa 1-6 细胞
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Targeting NAD+ metabolism of hepatocellular carcinoma cells by lenvatinib promotes M2 macrophages reverse polarization, suppressing the HCC progression (2023-05-19)
作者：Qingcan Sun:1.Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China. ; 2.State Key Laboratory of Organ Failure Research, Guangzhou, 510515, China. ; 3.Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Guangzhou, 510515, China. ; Mengying Shen:1.Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China. ; 2.State Key Laboratory of Organ Failure Research, Guangzhou, 510515, China. ; 3.Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Guangzhou, 510515, China. ; Subin Zhu:1.Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China. ; 2.State Key Laboratory of Organ Failure Research, Guangzhou, 510515, China. ; 3.Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Guangzhou, 510515, China. ; Yanxia Liao:1.Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou,
期刊：Targeting NAD+ metabolism of hepatocellular carcinoma cells by lenvatinib promotes M2 macrophages reverse polarization, suppressing the HCC progression
DOI：10.1007/s12072-023-10544-7
影响因子 ：6.6
引用产品： Li-7 细胞 , PLC/PRF/5 细胞 , SNU-182 细胞 , SNU-387 细胞 , RAW 264.7 细胞
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Silencing of keratin 15 impairs viability and mobility while facilitating the doxorubicin chemosensitivity by inactivating the β‑catenin pathway in liver cancer (2023-08-30)
作者：Junying Wang:1.Department of Interventional Radiology and Vascular Surgery, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210009, P.R. China. ; Guangyu Zhu:1.Department of Interventional Radiology and Vascular Surgery, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210009, P.R. China. ;
期刊：Silencing of keratin 15 impairs viability and mobility while facilitating the doxorubicin chemosensitivity by inactivating the β‑catenin pathway in liver cancer
影响因子 ：2.9
引用产品： HuH-7 细胞 , PLC/PRF/5 细胞 , Hep 3B2.1-7 细胞 , Hep G2 细胞 , Li-7 细胞 , RPMI-1640 培养基 , DMEM高糖 培养基 , 青霉素-链霉素溶液 (双抗)，100×
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VEZF1, destabilized by STUB1, affects cellular growth and metastasis of hepatocellular carcinoma by transcriptionally regulating PAQR4 (2022-10-14)
作者：Xiaobao Shi:1.Department of Radiology, The Fourth Affiliated Hospital of China Medical University, Shenyang, China. ; Pengfei Zhao:1.Department of Interventional Medicine, Shengjing Hospital of China Medical University, Shenyang, China. ; Gang Zhao:1.Department of Interventional Medicine, Shengjing Hospital of China Medical University, Shenyang, China. zhaog@sj-hospital.org. ;
期刊：VEZF1, destabilized by STUB1, affects cellular growth and metastasis of hepatocellular carcinoma by transcriptionally regulating PAQR4
DOI：10.1038/s41417-022-00540-8
影响因子 ：5.9
引用产品： SK-Hep-1 细胞 , PLC/PRF/5 细胞 , HuH-7 细胞 , Hep 3B2.1-7 细胞
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PAQR4 promotes the development of hepatocellular carcinoma by activating PI3K/AKT pathway (2010-01-20)
作者：Gang Zhao:1.Department of Radiology, Shengjing Hospital of China Medical University, Shenyang 110004, China. ; Xiaobao Shi:1.Department of Radiology, The Fourth Affiliated Hospital of China Medical University, Shenyang 110004, China. ; Zhanbo Sun:1.Department of Radiology, Shengjing Hospital of China Medical University, Shenyang 110004, China. ; Pengfei Zhao:1.Department of Radiology, Shengjing Hospital of China Medical University, Shenyang 110004, China. ; Zaiming Lu:1.Department of Radiology, Shengjing Hospital of China Medical University, Shenyang 110004, China. ;
期刊：PAQR4 promotes the development of hepatocellular carcinoma by activating PI3K/AKT pathway
影响因子 ：3.5
引用产品： HuH-7 细胞 , SK-Hep-1 细胞 , PLC/PRF/5 细胞
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