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产品概述
名称 | HCT-8 [HRT-18] (人回盲肠癌细胞) (STR鉴定正确) |
别称 | HCT-8; HCT8 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | HCT-8 [HRT-18]细胞与HRT-18细胞是一样的;角蛋白免疫过氧化物酶染色阳性。 |
年龄(性别) | 男性;67岁 |
组织来源 | 结肠;回盲肠结直肠腺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肠癌细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in nude mice(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^8 cells). |
基因表达情况 | carcinoembryonic antigen (CEA) 0.5 ng/10^6 cells/10 days; alkaline phosphatase. The cells are positive for keratin by immunoperoxidase staining. |
保藏机构 | ECACC; 90032006 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 17,25 D3S1358 17 D5S818 13 D7S820 10,11.3 D8S1179 15 D13S317 8,11 D16S539 12,13 D18S51 11,17 D19S433 14,16 D21S11 29,32.2 FGA 22 PentaD 9,14 PentaE 7,14 TH01 7,9.3 TPOX 8,11 vWA 18,19 D6S1043 11,13 D12S391 19,22 D2S441 13,15 -
STR鉴定图
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参考文献
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Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis (2024-01-17)
期刊:Acta Pharmaceutica Sinica B
DOI:10.1016/j.apsb.2024.01.006
影响因子 :14.5
引用产品: 特级胎牛血清 , LoVo 细胞 , HT-29 细胞 , HCT 116 细胞 , SW620 细胞 , RKO 细胞 , DLD-1 细胞 , HCT-8 [HRT-18] 细胞
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Discovery of a nitroaromatic nannocystin with potent in vivo anticancer activity against colorectal cancer by targeting AKT1 (2024-02-07)
期刊:ACTA PHARMACOLOGICA SINICA
DOI:10.1038/s41401-024-01231-w
影响因子 :8.2
引用产品: HCT 116 细胞 , HCT-8 [HRT-18] 细胞 , LoVo 细胞 , NCI-H1299 细胞 , NCI-H460 [H460] 细胞 , OV2008 细胞
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Huaier Regulates Oxaliplatin Resistance in Colorectal Cancer by Regulating Autophagy and Inhibiting the Wnt/β-catenin Signalling Pathway (2024-01-17)
期刊:Frontiers in Bioscience-Landmark
影响因子 :3.1
引用产品: RPMI-1640 培养基 , HCT-8 [HRT-18] 细胞 , 特级胎牛血清 , 青霉素-链霉素-两性霉素B溶液 (100×)
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Thalidomide suppresses migration and invasion of colorectal cancer cells by inhibiting HOXB7-mediated activation of the Wnt/β-catenin signaling pathway (2024-01-05)
期刊:Chemical Biology & Drug Design
影响因子 :3.0
引用产品: HCT-8 [HRT-18] 细胞 , SW480 [SW-480]细胞专用培养基 (L15) , Caco-2细胞专用培养基 , RPMI-1640 培养基 , 青霉素-链霉素溶液 (双抗),100× , 0.25%胰蛋白酶溶液 (溶于PBS)
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Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer (2023-11-14)
期刊:Science Bulletin
DOI:10.1016/j.scib.2023.11.027
影响因子 :18.9
引用产品: HCT-8 [HRT-18] 细胞
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The Active Fraction of Polyrhachis vicina Roger (AFPR) activates ERK to cause necroptosis in colorectal cancer (2023-04-12)
期刊:JOURNAL OF ETHNOPHARMACOLOGY
影响因子 :5.4
引用产品: SW1116 细胞 , HCT-8 [HRT-18] 细胞
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Phytochemical profile and biological activities of the essential oils in the aerial part and root of Saposhnikovia divaricata (2023-05-29)
期刊:Scientific Reports
DOI:10.1038/s41598-023-35656-w
影响因子 :4.6
引用产品: Hela 细胞 , HCT-8 [HRT-18] 细胞
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DEPDC1B is involved in the proliferation, metastasis, cell cycle arrest and apoptosis of colon cancer cells by regulating NUP37 (2023-05-16)
期刊:Molecular Medicine Reports
影响因子 :3.4
引用产品: SW620 细胞 , HCT-8 [HRT-18] 细胞 , HCT 116 细胞
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Development and validation of cuproptosis-related lncRNA signatures for prognosis prediction in colorectal cancer (2023-03-22)
期刊:BMC Medical Genomics
DOI:10.1186/s12920-023-01487-x
影响因子 :2.7
引用产品: FHC 细胞 , SW480 [SW-480] 细胞 , SW620 细胞 , HCT-8 [HRT-18] 细胞 , HT-29 细胞 , LoVo 细胞
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Hsa_Circ_0104206 is An Oncogenic circRNA in Colon Cancer by Targeting Mir-188–3p/CCNA2 Axis (2023-06-05)
期刊:HORMONE AND METABOLIC RESEARCH
影响因子 :2.2
引用产品: HCT-8 [HRT-18] 细胞 , SW620 细胞 , HCT 116 细胞 , SW480 [SW-480] 细胞 , DMEM高糖 培养基 , RPMI-1640 培养基 , 特级胎牛血清
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The feedback loop of ANKHD1/lncRNA MALAT1/YAP1 strengthens the radioresistance of CRC by activating YAP1/AKT signaling (2002-02-20)
期刊:Cell Death & Disease
DOI:10.1038/s41419-022-04554-w
影响因子 :9.0
引用产品: HCT 116 细胞 , HCT-8 [HRT-18] 细胞
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Site-directed late-stage diversification of macrocyclic nannocystins facilitating anticancer SAR and mode of action studies (2022-12-22)
期刊:RSC Medicinal Chemistry
影响因子 :3.5
引用产品: HCT 116 细胞 , HCT-8 [HRT-18] 细胞 , MDA-MB-231 细胞
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Aureusidin derivative CNQX inhibits chronic colitis inflammation and mucosal barrier damage by targeting myeloid differentiation 2 protein (2007-01-20)
期刊:Journal Of Cellular And Molecular Medicine
影响因子 :5.3
引用产品: HCT-8 [HRT-18] 细胞
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