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产品概述
名称 | HCT-8 [HRT-18] (人回盲肠癌细胞) (STR鉴定正确) |
别称 | HCT-8; HCT8 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | HCT-8 [HRT-18]细胞与HRT-18细胞是一样的;角蛋白免疫过氧化物酶染色阳性。 |
年龄(性别) | 男性;67岁 |
组织来源 | 结肠;回盲肠结直肠腺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肠癌细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in nude mice(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^8 cells). |
基因表达情况 | carcinoembryonic antigen (CEA) 0.5 ng/10^6 cells/10 days; alkaline phosphatase. The cells are positive for keratin by immunoperoxidase staining. |
保藏机构 | ECACC; 90032006 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 17,25 D3S1358 17 D5S818 13 D7S820 10,11.3 D8S1179 15 D13S317 8,11 D16S539 12,13 D18S51 11,17 D19S433 14,16 D21S11 29,32.2 FGA 22 PentaD 9,14 PentaE 7,14 TH01 7,9.3 TPOX 8,11 vWA 18,19 D6S1043 11,13 D12S391 19,22 D2S441 13,15 -
STR鉴定图
参考文献
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Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis (2024-01-17)
作者:Can Cheng:1.Department of General Surgery, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China. ; 2.Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China. ; Hanhui Yao:1.Department of General Surgery, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China. ; 2.Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China. ; Heng Li:1.Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and T
期刊:Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis
DOI:10.1016/j.apsb.2024.01.006
影响因子 :14.5
引用产品: 特级胎牛血清 , LoVo 细胞 , HT-29 细胞 , HCT 116 细胞 , SW620 细胞 , RKO 细胞 , DLD-1 细胞 , HCT-8 [HRT-18] 细胞
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Discovery of a nitroaromatic nannocystin with potent in vivo anticancer activity against colorectal cancer by targeting AKT1 (2024-02-07)
作者:Han Zhang:1.The State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300353, China. ; Fei Xie:1.The State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300353, China. ; Xiao-Ya Yuan:1.The State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300353, China. ; Xin-Tong Dai:1.The State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300353, China. ; Yun-Feng Tian:1.The State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300353, China. ; Ming-Ming Sun:1.The State Key Laboratory o
期刊:Discovery of a nitroaromatic nannocystin with potent in vivo anticancer activity against colorectal cancer by targeting AKT1
DOI:10.1038/s41401-024-01231-w
影响因子 :8.2
引用产品: HCT 116 细胞 , HCT-8 [HRT-18] 细胞 , LoVo 细胞 , NCI-H1299 细胞 , NCI-H460 [H460] 细胞 , OV2008 细胞
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Huaier Regulates Oxaliplatin Resistance in Colorectal Cancer by Regulating Autophagy and Inhibiting the Wnt/β-catenin Signalling Pathway (2024-01-17)
作者:Qi Feng:1.Department of Pathophysiology, Chengde Medical University, 067000 Chengde, Hebei, China. ; Mingyi Huo:1.Department of Pathophysiology, Chengde Medical University, 067000 Chengde, Hebei, China. ; Xiaoli Yan:1.Department of Pathophysiology, Chengde Medical University, 067000 Chengde, Hebei, China. ; Luwen Wen:1.Department of Pathophysiology, Chengde Medical University, 067000 Chengde, Hebei, China. ; Jining Zheng:1.Department of Pathophysiology, Chengde Medical University, 067000 Chengde, Hebei, China. ;
期刊:Huaier Regulates Oxaliplatin Resistance in Colorectal Cancer by Regulating Autophagy and Inhibiting the Wnt/β-catenin Signalling Pathway
影响因子 :3.1
引用产品: RPMI-1640 培养基 , HCT-8 [HRT-18] 细胞 , 特级胎牛血清 , 青霉素-链霉素-两性霉素B溶液 (100×)
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Thalidomide suppresses migration and invasion of colorectal cancer cells by inhibiting HOXB7-mediated activation of the Wnt/β-catenin signaling pathway (2024-01-05)
作者:Liyang Liu:1.Department of Anoretal, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China. ; Wusong Xue:1.Department of Anoretal, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China. ;
期刊:Thalidomide suppresses migration and invasion of colorectal cancer cells by inhibiting HOXB7-mediated activation of the Wnt/β-catenin signaling pathway
影响因子 :3.0
引用产品: HCT-8 [HRT-18] 细胞 , SW480 [SW-480]细胞专用培养基 (L15) , Caco-2细胞专用培养基 , RPMI-1640 培养基 , 青霉素-链霉素溶液 (双抗),100× , 0.25%胰蛋白酶溶液 (溶于PBS)
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Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer (2023-11-14)
作者:Binbin Zheng:1.Department of Urology, Shenzhen Institute of Translational Medicine, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, International Cancer Center of Shenzhen University, Shenzhen 518039, China; Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China. ; Liman Niu:1.Department of Urology, Shenzhen Institute of Translational Medicine, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, International Cancer Center of Shenzhen University, Shenzhen 518039, China; Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China. ; Haibo Xu:1.Department of Urology, Shenzhen Institute of Translational Medicine, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, International Cancer Center of Shenzhen University, Sh
期刊:Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer
DOI:10.1016/j.scib.2023.11.027
影响因子 :18.9
引用产品: HCT-8 [HRT-18] 细胞
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The Active Fraction of Polyrhachis vicina Roger (AFPR) activates ERK to cause necroptosis in colorectal cancer (2023-04-12)
作者:Dong-Mei Li:1.School of Chemistry & Pharmaceutical Sciences, State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin, 541004, China; Department of Pharmacology, Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Guangxi Institute of Chinese Medicine and Pharmaceutical Science, Nanning, 530022, China. ; Fu-Cui Zhu:1.Pharmaceutical College, Guangxi Medical University, Nanning, 530021, China. ; Jie Wei:1.Department of Pharmacology, Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Guangxi Institute of Chinese Medicine and Pharmaceutical Science, Nanning, 530022, China. ; Jia-Xiu Xie:1.Department of Pharmacology, Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Guangxi Institute of Chinese Medicine and Pharmaceutical Science, Nanning, 530022, China. ; Jun-Hui He:1.Department of Pharmacology, Guangxi Key Laboratory of Traditional Chinese Medicine Qua
期刊:The Active Fraction of Polyrhachis vicina Roger (AFPR) activates ERK to cause necroptosis in colorectal cancer
影响因子 :5.4
引用产品: SW1116 细胞 , HCT-8 [HRT-18] 细胞
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Phytochemical profile and biological activities of the essential oils in the aerial part and root of Saposhnikovia divaricata (2023-05-29)
作者:Bing Li:1.College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, 050200, China. ; 2.Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, Shijiazhuang, 050200, China. ; 3.International Joint Research Center on Resource Utilization and Quality Evaluation of Traditional Chinese Medicine of Hebei Province, Shijiazhuang, 050200, China. ; Zhenmin Yang:1.College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, 050200, China. ; 2.Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, Shijiazhuang, 050200, China. ; Fuying Mao:1.Experimental Center, Hebei University of Chinese Medicine, Shijiazhuang, 050200, China. ; Qian Wang:1.College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, 050200, China. ; Huiyong Fang:1.College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, 050200, China. ; Xian Gu:1.College of Pharmacy, Hebei University of Chinese Medicine, Sh
期刊:Phytochemical profile and biological activities of the essential oils in the aerial part and root of Saposhnikovia divaricata
DOI:10.1038/s41598-023-35656-w
影响因子 :4.6
引用产品: Hela 细胞 , HCT-8 [HRT-18] 细胞
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DEPDC1B is involved in the proliferation, metastasis, cell cycle arrest and apoptosis of colon cancer cells by regulating NUP37 (2023-05-16)
作者:Hui Xiong:1.Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China. ; Yun Li:1.Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China. ; Manhua Liu:1.Department of General Surgery, The First Affiliated Hospital of Nanchang University Jing'an County People's Hospital, Jing'an, Shanghai 330600, P.R. China. ;
期刊:DEPDC1B is involved in the proliferation, metastasis, cell cycle arrest and apoptosis of colon cancer cells by regulating NUP37
影响因子 :3.4
引用产品: SW620 细胞 , HCT-8 [HRT-18] 细胞 , HCT 116 细胞
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Development and validation of cuproptosis-related lncRNA signatures for prognosis prediction in colorectal cancer (2023-03-22)
作者:Lin Pang:1.Department of Colorectal and Anal Surgery, The Fifth Clinical Medical College of Shanxi Medical University, Taiyuan, 030012, China. ; 2.Department of Health Statistics, School of Public Health, Shanxi Medical University, Taiyuan, 030001, China. ; Qingqing Wang:1.Department of Health Statistics, School of Public Health, Shanxi Medical University, Taiyuan, 030001, China. ; Lingxiao Wang:1.Department of Colorectal and Anal Surgery, The Fifth Clinical Medical College of Shanxi Medical University, Taiyuan, 030012, China. ; Zhen Hu:1.Department of Colorectal and Anal Surgery, The Fifth Clinical Medical College of Shanxi Medical University, Taiyuan, 030012, China. ; Chong Yang:1.Department of Colorectal and Anal Surgery, The Fifth Clinical Medical College of Shanxi Medical University, Taiyuan, 030012, China. ; Yiqun Li:1.Department of Colorectal and Anal Surgery, The Fifth Clinical Medical College of Shanxi Medical University, Taiyuan, 030012, China. ; Zhenqi Wang:1.Departmen
期刊:Development and validation of cuproptosis-related lncRNA signatures for prognosis prediction in colorectal cancer
DOI:10.1186/s12920-023-01487-x
影响因子 :2.7
引用产品: FHC 细胞 , SW480 [SW-480] 细胞 , SW620 细胞 , HCT-8 [HRT-18] 细胞 , HT-29 细胞 , LoVo 细胞
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Hsa_Circ_0104206 is An Oncogenic circRNA in Colon Cancer by Targeting Mir-188–3p/CCNA2 Axis (2023-06-05)
作者:Zhong Li:1.Department of Proctology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ; Quanfu Li:1.Department of Proctology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ; Zhuo Chen:1.Department of Anesthesiology, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan, China. ;
期刊:Hsa_Circ_0104206 is An Oncogenic circRNA in Colon Cancer by Targeting Mir-188–3p/CCNA2 Axis
影响因子 :2.2
引用产品: HCT-8 [HRT-18] 细胞 , SW620 细胞 , HCT 116 细胞 , SW480 [SW-480] 细胞 , DMEM高糖 培养基 , RPMI-1640 培养基 , 特级胎牛血清
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The feedback loop of ANKHD1/lncRNA MALAT1/YAP1 strengthens the radioresistance of CRC by activating YAP1/AKT signaling (2002-02-20)
作者:Ping-An Yao:1.Department of General Surgery, Second Affiliated Hospital of Soochow University, Suzhou, 215004, China. ; Yong Wu:1.Department of General Surgery, Second Affiliated Hospital of Soochow University, Suzhou, 215004, China. ; Kui Zhao:1.Department of General Surgery, Second Affiliated Hospital of Soochow University, Suzhou, 215004, China. ; Yecheng Li:1.Department of General Surgery, Second Affiliated Hospital of Soochow University, Suzhou, 215004, China. ; Jianping Cao:1.School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, 215123, China. jpcao@suda.edu.cn. ; 2.State Key Laboratory of Radiation Medicine and Protection and Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China. jpcao@suda.edu.cn. ; Chungen Xing:1.Department of General Surgery, Second Affiliated Hospital of Soochow University, Suzhou, 215004, China. xingcg@suda.edu.cn. ;
期刊:The feedback loop of ANKHD1/lncRNA MALAT1/YAP1 strengthens the radioresistance of CRC by activating YAP1/AKT signaling
DOI:10.1038/s41419-022-04554-w
影响因子 :9.0
引用产品: HCT 116 细胞 , HCT-8 [HRT-18] 细胞
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Site-directed late-stage diversification of macrocyclic nannocystins facilitating anticancer SAR and mode of action studies (2022-12-22)
作者:Han Zhang:1.The State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University Tianjin People's Republic of China zhangweicheng@nankai.edu.cn changliangshan@nankai.edu.cn. ; Yunfeng Tian:1.The State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University Tianjin People's Republic of China zhangweicheng@nankai.edu.cn changliangshan@nankai.edu.cn. ; Xiaoya Yuan:1.The State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University Tianjin People's Republic of China zhangweicheng@nankai.edu.cn changliangshan@nankai.edu.cn. ; Fei Xie:1.The State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University Tianjin People's Republic of China zhangweicheng@nankai.edu.c
期刊:Site-directed late-stage diversification of macrocyclic nannocystins facilitating anticancer SAR and mode of action studies
影响因子 :3.5
引用产品: HCT 116 细胞 , HCT-8 [HRT-18] 细胞 , MDA-MB-231 细胞
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Aureusidin derivative CNQX inhibits chronic colitis inflammation and mucosal barrier damage by targeting myeloid differentiation 2 protein (2007-01-20)
作者:Yi Yang:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ; Yongjia Sheng:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ; Jin Wang:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ; Xiaohong Zhou:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ; Qiaobing Guan:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ; Heping Shen:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ; Wenyan Li:1.Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ; Shuiliang Ruan:1.Department of Center Laboratory, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. ;
期刊:Aureusidin derivative CNQX inhibits chronic colitis inflammation and mucosal barrier damage by targeting myeloid differentiation 2 protein
影响因子 :5.3
引用产品: HCT-8 [HRT-18] 细胞
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