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产品概述
名称 | NCI-H520 (人肺鳞癌细胞) (STR鉴定正确) |
别称 | H520; H-520; NCI-HUT-520; NCIH520 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | NCI-H520细胞是由Gazdar AF等在1982年建系而来,源于鳞状细胞肺癌组织;NCI-H520细胞p53 mRNA表达水平较正常肺组织低,但是没有大的结构DNA的异常。NCI-H520细胞角蛋白、波形蛋白阳性,神经丝三联蛋白阴性;NCI-H520细胞在软琼脂中可形成克隆。 |
组织来源 | 肺 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~32-60 hours |
致瘤性 | Yes, in nude mice inoculated subcutaneously with 1×10^7 cells (Tumors developed within 21 days at 100% frequency (5/5)). |
保藏机构 | ATCC; HTB-182 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10 D2S1338 18,23 D3S1358 16 D5S818 12,13 D7S820 8,12 D8S1179 14,16,17 D13S317 10,11 D16S539 8,13 D18S51 17 D19S433 13,14 D21S11 30 FGA 22 PentaD 13 PentaE 5,14 TH01 10 TPOX 8 vWA 18,19 D6S1043 12,18 D12S391 21 D2S441 10,11 -
STR鉴定图
参考文献
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Single-cell transcriptomic sequencing data reveal aberrant DNA methylation in SMAD3 promoter region in tumor-associated fibroblasts affecting molecular mechanism of radiosensitivity in non-small cell lung cancer (2024-03-16)
作者:Fushi Han:1.Department of Medical Imaging, Tongji Hospital, School of Medicine, Tongji University, No. 389, Xincun Road, Putuo District, Shanghai, 200065, People's Republic of China. ; 2.Institute of Medical Imaging Artificial Intelligence, Tongji University School of Medicine, Shanghai, 200065, China. ; Shuzhen Chen:1.Department of Nuclear Medicine, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, People's Republic of China. ; Kangwei Zhang:1.Department of Medical Imaging, Tongji Hospital, School of Medicine, Tongji University, No. 389, Xincun Road, Putuo District, Shanghai, 200065, People's Republic of China. ; 2.Institute of Medical Imaging Artificial Intelligence, Tongji University School of Medicine, Shanghai, 200065, China. ; Kunming Zhang:1.Department of Internal Medicine, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, People's Republic of China. ; Meng Wang:1.Department of Radiotherapy, Tongji Hospital, Tongji University Sch
期刊:Single-cell transcriptomic sequencing data reveal aberrant DNA methylation in SMAD3 promoter region in tumor-associated fibroblasts affecting molecular mechanism of radiosensitivity in non-small cell lung cancer
DOI:10.1186/s12967-024-05057-2
影响因子 :7.4
引用产品: NCI-H2228 [H2228; H-2228] 细胞 , NCI-H520 细胞 , 16HBE 细胞
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Combined Inhibition of UBE2C and PLK1 Reduce Cell Proliferation and Arrest Cell Cycle by Affecting ACLY in Pan-Cancer (2023-10-27)
作者:Keying Liang:1.School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China. ; Qian Wang:1.School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China. ; Li Qiu:1.School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China. ; Xiaocheng Gong:1.School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China. ; Zixi Chen:1.School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China. ; Haibo Zhang:1.School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China. ; Ke Ding:1.School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China. ; Yunfei Liu:1.School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China. ; Ji
期刊:Combined Inhibition of UBE2C and PLK1 Reduce Cell Proliferation and Arrest Cell Cycle by Affecting ACLY in Pan-Cancer
影响因子 :5.6
引用产品: HCC1937 细胞 , NCI-H520 细胞 , SNU-387 细胞 , SNU-182 细胞 , BEAS-2B 细胞 , MCF 10A细胞专用培养基
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Celastrus orbiculatus Thunb. extract targeting DJ-1 inhibits non-small cell lung cancer invasion and metastasis through mitochondrial-induced ROS accumulation (2023-07-20)
作者:Xiaochen Ni:1.Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, PR China. Electronic address: nxc19970103@163.com. ; Shilong Yu:1.Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou, 225001, PR China; Yangzhou Hospital of Traditional Chinese Medicine, Yangzhou, 225001, PR China. Electronic address: yushilong1992@163.com. ; Xiaomin Jiang:1.The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou, 225001, PR China; Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou, 225001, PR China. Electronic address: 15050735732@139.com. ; Feng Wu:1.Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, PR China. Electronic address: yzwufeng888@163.com. ; Jun Zhou:1.D
期刊:Celastrus orbiculatus Thunb. extract targeting DJ-1 inhibits non-small cell lung cancer invasion and metastasis through mitochondrial-induced ROS accumulation
影响因子 :5.4
引用产品: NCI-H226 [H226] 细胞 , NCI-H520 细胞 , HCC827 细胞
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Development and validation of a N6 methylation regulator-related gene signature for prognostic and immune response prediction in non-small cell lung cancer (2023-07-15)
作者:Xiang Li:1.Qilu Hospital of Shandong University Jinan 250000, Shandong, China. ; 2.Cheeloo College of Medicine, Shandong University Jinan 250000, Shandong, China. ; Jinlong Ma:1.Qilu Hospital of Shandong University Jinan 250000, Shandong, China. ; 2.Cheeloo College of Medicine, Shandong University Jinan 250000, Shandong, China. ; Zhenqian Sun:1.Qilu Hospital of Shandong University Jinan 250000, Shandong, China. ; 2.Cheeloo College of Medicine, Shandong University Jinan 250000, Shandong, China. ; Na Li:1.Mechanics Laboratory, Binzhou Medical University Yantai 250000, Shandong, China. ; Guangjun Jiao:1.Qilu Hospital of Shandong University Jinan 250000, Shandong, China. ; 2.Cheeloo College of Medicine, Shandong University Jinan 250000, Shandong, China. ; Tianqi Zhang:1.Qilu Medical University Zibo 250000, Shandong, China. ; Hongxin Cao:1.Department of Medical Oncology, Qilu Hospital of Shandong University Jinan 250000, Shandong, China. ; 2.Key Laboratory of Chemical Biology (Ministr
期刊:Development and validation of a N6 methylation regulator-related gene signature for prognostic and immune response prediction in non-small cell lung cancer
DOI:PMID:37560004
影响因子 :5.3
引用产品: A549 [A-549] 细胞 , NCI-H1299 细胞 , NCI-H520 细胞
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Regulation of tumorigenesis and ferroptosis in non-small cell lung cancer by a novel BBOX1-AS1/miR-326/PROM2 axis (2023-08-28)
作者:Jinlu An:1.Department of Geriatric Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, 450052, China. ; Jiang Shi:1.Department of Geriatric Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, 450052, China. shijiangdoc@163.com. ; Chao Yang:1.Department of Geriatric Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, 450052, China. ; Junfang Luo:1.Department of Geriatric Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, 450052, China. ; Yuning Li:1.Department of Geriatric Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, 450052, China. ; Jie Ren:1.Department of Geriatric Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzh
期刊:Regulation of tumorigenesis and ferroptosis in non-small cell lung cancer by a novel BBOX1-AS1/miR-326/PROM2 axis
DOI:10.1007/s11010-023-04837-6
影响因子 :4.3
引用产品: BEAS-2B 细胞 , A549 [A-549] 细胞 , SK-MES-1 细胞 , NCI-H1299 细胞 , NCI-H520 细胞 , Ham's F-12K 培养基 , MEM (含NEAA) 培养基 , RPMI-1640 培养基 , DMEM高糖 培养基
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Resveratrol improves the cytotoxic effect of CD8 +T cells in the tumor microenvironment by regulating HMMR/Ferroptosis in lung squamous cell carcinoma (2023-03-15)
作者:Gao Shan:1.Department of Thoracic Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China; Institute of Organ Transplantation, Health Science Center of Xi'an Jiaotong University, Xi'an 710061, China. Electronic address: gaoshan_thoracic@xjtufh.edu.cn. ; Kang Minchao:1.Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China. Electronic address: Kmc2022@163.com. ; Wang Jizhao:1.Department of Thoracic Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. Electronic address: wangjz1104@xjtu.edu.cn. ; Zhao Rui:1.Department of Thoracic Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. Electronic address: rainzhao0525@foxmail.com. ; Zhang Guangjian:1.Department of Thoracic Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. Electronic address: michael8039@163.com. ; Zheng Jin:1.Department of Renal Transplantation, First Affil
期刊:Resveratrol improves the cytotoxic effect of CD8 +T cells in the tumor microenvironment by regulating HMMR/Ferroptosis in lung squamous cell carcinoma
DOI:10.1016/j.jpba.2023.115346
影响因子 :3.4
引用产品: NCI-H520 细胞
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Hydrogen Peroxide-Inducible PROTACs for Targeted Protein Degradation in Cancer Cells (2023-07-18)
作者:Dehao Yu:1.Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, The Province and Ministry Co-sponsored Collaborative Innovation Center, for Medical Epigenetics, Tianjin Medical University, Tianjin, 300070, China. ; Heli Fan:1.Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, The Province and Ministry Co-sponsored Collaborative Innovation Center, for Medical Epigenetics, Tianjin Medical University, Tianjin, 300070, China. ; Zhili Zhou:1.Key Laboratory of Immune Microenvironment and Disease, Ministry of Education), Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, China. ; Ying Zhang:1.Key Laboratory of Immune Microenvironment and Disease, Ministry of Education), Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, China. ; Jing Sun:1.Tianj
期刊:Hydrogen Peroxide-Inducible PROTACs for Targeted Protein Degradation in Cancer Cells
影响因子 :3.2
引用产品: A549 [A-549] 细胞 , NCI-H1299 细胞 , NCI-H1975 细胞 , NCI-H520 细胞 , WI-38 细胞 , HULEC-5a 细胞
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LINC00668 promoted non-small lung cancer progression by miR-518c-3p/TRIP4 axis (2023-09-05)
作者:Zhibin Lu:1.The First Clinical Medical College of Nanjing Medical University, Nanjing, Jiangsu, China. ; 2.Nanjing Pukou People's Hospital, Nanjing, Jiangsu, China. ; 3.The First Clinical Medical College of Nanjing Medical University, Nanjing, Jiangsu, China. ; Zhichao Xiao:1.Nanjing Pukou People's Hospital, Nanjing, Jiangsu, China. ; 2.The First Clinical Medical College of Nanjing Medical University, Nanjing, Jiangsu, China. ; Qi Wang:1.The First Clinical Medical College of Nanjing Medical University, Nanjing, Jiangsu, China. ; 2.Department of Thoracic Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China. ; 3.The First Clinical Medical College of Nanjing Medical University, Nanjing, Jiangsu, China. ; Chunfeng Pan:1.Department of Thoracic Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China. ; Yang Xia:1.Department of Thoracic Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanji
期刊:LINC00668 promoted non-small lung cancer progression by miR-518c-3p/TRIP4 axis
影响因子 :3.1
引用产品: BEAS-2B 细胞 , NCI-H1703 细胞 , SK-MES-1 细胞 , NCI-H520 细胞
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Restraint of chaperonin containing T-complex protein-1 subunit 3 has antitumor roles in non-small cell lung cancer via affection of YAP1 (2002-02-20)
作者:Hongyang Shi:1.Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi 71004, China. Electronic address: shihongyanghy@163.com. ; Yonghong Zhang:1.Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi 71004, China. ; Yu Wang:1.Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi 71004, China. ; Ping Fang:1.Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi 71004, China. ; Yun Liu:1.Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi 71004, China. ; Wei Li:1.Department of Respiratory and Critical Care Medicine,
期刊:Restraint of chaperonin containing T-complex protein-1 subunit 3 has antitumor roles in non-small cell lung cancer via affection of YAP1
DOI:10.1016/j.taap.2022.115926
影响因子 :4.5
引用产品: NCI-H1688 , BEAS-2B , HuH-7 细胞 , NCI-H520 细胞 , NCI-H1299 细胞
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Palbociclib Induces the Apoptosis of Lung Squamous Cell Carcinoma Cells via RB-Independent STAT3 Phosphorylation (2022-08-18)
作者:Wenjing Xiang:1.School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China. ; Wanchen Qi:1.The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China. ; Huayu Li:1.School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China. ; Jia Sun:1.Center for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou 510006, China. ; Chao Dong:1.School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China. ; Haojie Ou:1.School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China. ; Bing Liu:1.School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China. ; 2.Guangdong Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou 510006, China. ; 3.Guangzhou Key Laboratory of Construction and Application of New Drug Screening Model Systems, Guangdong Pharmaceutical University, Guangzhou 510006, China. ;
期刊:Palbociclib Induces the Apoptosis of Lung Squamous Cell Carcinoma Cells via RB-Independent STAT3 Phosphorylation
影响因子 :3.1
引用产品: NCI-H520 细胞
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Silencing ZIC2 abrogates tumorigenesis and anoikis resistance of non-small cell lung cancer cells by inhibiting Src/FAK signaling (2005-01-20)
作者:Aibin Liu:1.Department of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China. ; 2.National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China. ; Huayan Xie:1.Department of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China. ; Ronggang Li:1.Department of Pathology, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen University, Jiangmen 529030, China. ; Liangliang Ren:1.Clinical Experimental Center, Jiangmen Key Laboratory of Clinical Biobanks and Translational Research, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen University, Jiangmen 529030, China. ; Baishuang Yang:1.Department of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China. ; Longxia Dai:1.Department of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China. ; Wenjie Lu:1.Clinical Experimental Center, Jian
期刊:Silencing ZIC2 abrogates tumorigenesis and anoikis resistance of non-small cell lung cancer cells by inhibiting Src/FAK signaling
DOI:10.1016/j.omto.2021.05.008
影响因子 :5.7
引用产品: NCI-H1975 细胞 , NCI-H1395 细胞 , NCI-H520 细胞 , A549/DDP 细胞 , NCI-H1299 细胞
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Knockdown of lysine (K)-specific demethylase 2B KDM2B inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway (2012-01-20)
作者:Zhonghai Xie:1.Department of Thoracic Surgery, Huzhou Central Hospital, Huzhou, Zhejiang Province, China. ; Hongwei Li:1.Department of Thoracic Surgery, Huzhou Central Hospital, Huzhou, Zhejiang Province, China. ; Jin Zang:1.Department of Thoracic Surgery, Huzhou Central Hospital, Huzhou, Zhejiang Province, China. ;
期刊:Knockdown of lysine (K)-specific demethylase 2B KDM2B inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway
DOI:10.1080/21655979.2021.2005931
影响因子 :4.9
引用产品: BEAS-2B , SK-MES-1 细胞 , NCI-H520 细胞
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TMED3 exerts a protumor function in non-small cell lung cancer by enhancing the Wnt/β-catenin pathway via regulation of AKT (2011-01-20)
作者:Danjie Zhang:1.Department of Thoracic Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, 157 Xiwu Road, Xi'an 710004, China. ; Liangzhang Sun:1.Department of Thoracic Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, 157 Xiwu Road, Xi'an 710004, China. ; Jin Zhang:1.Department of Thoracic Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, 157 Xiwu Road, Xi'an 710004, China. Electronic address: zhangjin9605@163.com. ;
期刊:TMED3 exerts a protumor function in non-small cell lung cancer by enhancing the Wnt/β-catenin pathway via regulation of AKT
DOI:10.1016/j.taap.2021.115793
影响因子 :3.8
引用产品: BEAS-2B , A549 [A-549] 细胞 , NCI-H226 [H226] 细胞 , NCI-H520 细胞 , NCI-H1975 细胞
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Calponin 3 suppresses proliferation, migration and invasion of non‑small cell lung cancer cells (2007-01-20)
作者:Chenglin Yang:1.Traditional Chinese Medicine Department, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510632, P.R. China. ; Shiping Zhu:1.Traditional Chinese Medicine Department, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510632, P.R. China. ; Weifeng Feng:1.Traditional Chinese Medicine Department, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510632, P.R. China. ; Xuexin Chen:1.Department of Respiratory and Critical Care Medicine, The General Hospital of Yima Coal Industry Group Co. Ltd., Yima, Henan 472300, P.R. China. ;
期刊:Calponin 3 suppresses proliferation, migration and invasion of non‑small cell lung cancer cells
影响因子 :2.9
引用产品: PC-9 细胞 , NCI-H1975 细胞 , NCI-H520 细胞
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Tambulin Targets Histone Deacetylase 1 Inhibiting Cell Growth and Inducing Apoptosis in Human Lung Squamous Cell Carcinoma (2008-00-20)
作者:Wuming Wang:1.Department of Thoracic Surgery, Jiangxi Provincial Chest Hospital, Nanchang, China. ; Yuzhen Liu:1.Department of Thoracic Surgery, Jiangxi Provincial Chest Hospital, Nanchang, China. ; Long Zhao:1.Department of Thoracic Surgery, Jiangxi Provincial Chest Hospital, Nanchang, China. ;
期刊:Tambulin Targets Histone Deacetylase 1 Inhibiting Cell Growth and Inducing Apoptosis in Human Lung Squamous Cell Carcinoma
影响因子 :5.6
引用产品: BEAS-2B , NCI-H226 [H226] 细胞 , NCI-H520 细胞
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SLC6A8 is involved in the progression of non-small cell lung cancer through the Notch signaling pathway (2010-00-20)
作者:Yan Feng:1.Department of Respiratory Medicine, Qingdao Municipal Hospital, Qingdao University, Qingdao, China. ; Xiangyu Guo:1.Department of Respiratory Medicine, Qingdao Municipal Hospital, Qingdao University, Qingdao, China. ; Huaping Tang:1.Department of Respiratory Medicine, Qingdao Municipal Hospital, Qingdao University, Qingdao, China. ;
期刊:SLC6A8 is involved in the progression of non-small cell lung cancer through the Notch signaling pathway
影响因子 :3.6
引用产品: NCI-H1975 细胞 , NCI-H520 细胞 , NCI-H1299 细胞 , A549 [A-549] 细胞
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