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产品概述
名称 | NCI-H520 (人肺鳞癌细胞) (STR鉴定正确) |
别称 | H520; H-520; NCI-HUT-520; NCIH520 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | NCI-H520细胞是由Gazdar AF等在1982年建系而来,源于鳞状细胞肺癌组织;NCI-H520细胞p53 mRNA表达水平较正常肺组织低,但是没有大的结构DNA的异常。NCI-H520细胞角蛋白、波形蛋白阳性,神经丝三联蛋白阴性;NCI-H520细胞在软琼脂中可形成克隆。 |
组织来源 | 肺 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~32-60 hours |
致瘤性 | Yes, in nude mice inoculated subcutaneously with 1×10^7 cells (Tumors developed within 21 days at 100% frequency (5/5)). |
保藏机构 | ATCC; HTB-182 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10 D2S1338 18,23 D3S1358 16 D5S818 12,13 D7S820 8,12 D8S1179 14,16,17 D13S317 10,11 D16S539 8,13 D18S51 17 D19S433 13,14 D21S11 30 FGA 22 PentaD 13 PentaE 5,14 TH01 10 TPOX 8 vWA 18,19 D6S1043 12,18 D12S391 21 D2S441 10,11 -
STR鉴定图
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参考文献
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Single-cell transcriptomic sequencing data reveal aberrant DNA methylation in SMAD3 promoter region in tumor-associated fibroblasts affecting molecular mechanism of radiosensitivity in non-small cell lung cancer (2024-03-16)
期刊:Journal of Translational Medicine
DOI:10.1186/s12967-024-05057-2
影响因子 :7.4
引用产品: NCI-H2228 [H2228; H-2228] 细胞 , NCI-H520 细胞 , 16HBE 细胞
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Combined Inhibition of UBE2C and PLK1 Reduce Cell Proliferation and Arrest Cell Cycle by Affecting ACLY in Pan-Cancer (2023-10-27)
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 :5.6
引用产品: HCC1937 细胞 , NCI-H520 细胞 , SNU-387 细胞 , SNU-182 细胞 , BEAS-2B 细胞 , MCF 10A细胞专用培养基
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Celastrus orbiculatus Thunb. extract targeting DJ-1 inhibits non-small cell lung cancer invasion and metastasis through mitochondrial-induced ROS accumulation (2023-07-20)
期刊:JOURNAL OF ETHNOPHARMACOLOGY
影响因子 :5.4
引用产品: NCI-H226 [H226] 细胞 , NCI-H520 细胞 , HCC827 细胞
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Development and validation of a N6 methylation regulator-related gene signature for prognostic and immune response prediction in non-small cell lung cancer (2023-07-15)
期刊:American Journal of Cancer Research
DOI:PMID:37560004
影响因子 :5.3
引用产品: A549 [A-549] 细胞 , NCI-H1299 细胞 , NCI-H520 细胞
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Regulation of tumorigenesis and ferroptosis in non-small cell lung cancer by a novel BBOX1-AS1/miR-326/PROM2 axis (2023-08-28)
期刊:MOLECULAR AND CELLULAR BIOCHEMISTRY
DOI:10.1007/s11010-023-04837-6
影响因子 :4.3
引用产品: BEAS-2B 细胞 , A549 [A-549] 细胞 , SK-MES-1 细胞 , NCI-H1299 细胞 , NCI-H520 细胞 , Ham's F-12K 培养基 , MEM (含NEAA) 培养基 , RPMI-1640 培养基 , DMEM高糖 培养基
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Resveratrol improves the cytotoxic effect of CD8 +T cells in the tumor microenvironment by regulating HMMR/Ferroptosis in lung squamous cell carcinoma (2023-03-15)
期刊:JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
DOI:10.1016/j.jpba.2023.115346
影响因子 :3.4
引用产品: NCI-H520 细胞
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Hydrogen Peroxide-Inducible PROTACs for Targeted Protein Degradation in Cancer Cells (2023-07-18)
期刊:CHEMBIOCHEM
影响因子 :3.2
引用产品: A549 [A-549] 细胞 , NCI-H1299 细胞 , NCI-H1975 细胞 , NCI-H520 细胞 , WI-38 细胞 , HULEC-5a 细胞
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LINC00668 promoted non-small lung cancer progression by miR-518c-3p/TRIP4 axis (2023-09-05)
期刊:Cancer Biomarkers
影响因子 :3.1
引用产品: BEAS-2B 细胞 , NCI-H1703 细胞 , SK-MES-1 细胞 , NCI-H520 细胞
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Restraint of chaperonin containing T-complex protein-1 subunit 3 has antitumor roles in non-small cell lung cancer via affection of YAP1 (2002-02-20)
期刊:Toxicology And Applied Pharmacology
DOI:10.1016/j.taap.2022.115926
影响因子 :4.5
引用产品: NCI-H1688 , BEAS-2B , HuH-7 细胞 , NCI-H520 细胞 , NCI-H1299 细胞
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Palbociclib Induces the Apoptosis of Lung Squamous Cell Carcinoma Cells via RB-Independent STAT3 Phosphorylation (2022-08-18)
期刊:Current Oncology
影响因子 :3.1
引用产品: NCI-H520 细胞
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Silencing ZIC2 abrogates tumorigenesis and anoikis resistance of non-small cell lung cancer cells by inhibiting Src/FAK signaling (2005-01-20)
期刊:Molecular Therapy-Oncolytics
DOI:10.1016/j.omto.2021.05.008
影响因子 :5.7
引用产品: NCI-H1975 细胞 , NCI-H1395 细胞 , NCI-H520 细胞 , A549/DDP 细胞 , NCI-H1299 细胞
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Knockdown of lysine (K)-specific demethylase 2B KDM2B inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway (2012-01-20)
期刊:Bioengineered
DOI:10.1080/21655979.2021.2005931
影响因子 :4.9
引用产品: BEAS-2B , SK-MES-1 细胞 , NCI-H520 细胞
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TMED3 exerts a protumor function in non-small cell lung cancer by enhancing the Wnt/β-catenin pathway via regulation of AKT (2011-01-20)
期刊:Toxicology And Applied Pharmacology
DOI:10.1016/j.taap.2021.115793
影响因子 :3.8
引用产品: BEAS-2B , A549 [A-549] 细胞 , NCI-H226 [H226] 细胞 , NCI-H520 细胞 , NCI-H1975 细胞
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Calponin 3 suppresses proliferation, migration and invasion of non‑small cell lung cancer cells (2007-01-20)
期刊:Oncology Letters
影响因子 :2.9
引用产品: PC-9 细胞 , NCI-H1975 细胞 , NCI-H520 细胞
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Tambulin Targets Histone Deacetylase 1 Inhibiting Cell Growth and Inducing Apoptosis in Human Lung Squamous Cell Carcinoma (2008-00-20)
期刊:Frontiers in Pharmacology
影响因子 :5.6
引用产品: BEAS-2B , NCI-H226 [H226] 细胞 , NCI-H520 细胞
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SLC6A8 is involved in the progression of non-small cell lung cancer through the Notch signaling pathway (2010-00-20)
期刊:Annals of Translational Medicine
影响因子 :3.6
引用产品: NCI-H1975 细胞 , NCI-H520 细胞 , NCI-H1299 细胞 , A549 [A-549] 细胞
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