产品中心Cell Resources
联系我们CONTACT US
400-999-210024小时服务热线
产品概述
| 名称 | 3T3-L1 (小鼠胚胎成纤维细胞) (STR鉴定正确) |
| 别称 | 3T3 L1; 3T3L1; 3T3-L1 ad; NIH-3T3-L1; NIH3T3-L1 |
| 种属 | 小鼠 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:5 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | 3T3-L1细胞是通过克隆分离得到的3T3(swiss小白鼠)的连续亚株。当3T3-L1细胞从快速分裂到长满且接触抑制时，3T3-L1细胞经过前脂肪向脂肪样逆转。培养液中，高血清含量可以促进3T3-L1细胞内脂肪的积累。 |
| 年龄（性别） | 胚胎 |
| 组织来源 | 胚胎 |
| 细胞类型 | 自发永生化细胞 |
| 生物安全等级 | BSL-1 |
| 受体表达情况 | insulin, expressed |
| 保藏机构 | ATCC; CL-173 ATCC; CCL-92.1BCRC; 60159 |
所用细胞：3T3-L1 (小鼠胚胎成纤维细胞) ；货号：CL-0006
细胞代次：复苏后P5代
所用诱导试剂：3T3-L1 (小鼠胚胎成纤维细胞) 成脂诱导分化培养基；货号：PD-031
STR鉴定
-
STR位点信息
18-3 17,21 6-7 12,15 5-5 13,15 X-1 26 1-2 13 7-1 25.2,29 8-1 15,16 1-1 10,15 19-2 12 15-3 20.3 12-1 19 6-4 16 4-2 19.3 3-2 14 2-1 9 13-1 15.1
11-2 15 17-2 12,15 -
STR鉴定图
-
参考文献
-
Antitumor Effects of Carrier-Free Functionalized Lignin Materials on Human Hepatocellular Carcinoma (HepG2) Cells (2024-01-23)
期刊：ACS Nano
影响因子 ：17.1
引用产品： Hep G2 细胞 , 3T3-L1 细胞 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100× , MEMα 培养基 , DMEM高糖 培养基
-
Myeloid-derived grancalcin instigates obesity-induced insulin resistance and metabolic inflammation in male mice (2024-01-02)
期刊：Nature Communications
DOI：10.1038/s41467-023-43787-x
影响因子 ：16.6
引用产品： DMEM高糖 培养基 , 3T3-L1 细胞 , 293T [HEK-293T] 细胞
-
Silica-induced macrophage pyroptosis propels pulmonary fibrosis through coordinated activation of relaxin and osteoclast differentiation signaling to reprogram fibroblasts (2024-02-19)
期刊：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI：10.1016/j.ecoenv.2024.116106
影响因子 ：6.8
引用产品： J774A.1 细胞 , 3T3-L1 细胞
-
Insights into metabolic characteristics and biological activity changes in Zangju (Citrus reticulata cv. Manau Gan) peel at different maturity stages through UPLC–MS/MS-based metabolomics (2024-02-05)
期刊：Food Chemistry-X
DOI：10.1016/j.fochx.2024.101197
影响因子 ：6.1
引用产品： 3T3-L1 细胞 , DMEM高糖 培养基 , 特级胎牛血清
-
Alisol B 23-acetate promotes white adipose tissue browning to mitigate high-fat diet-induced obesity by regulating mTOR-SREBP1 signaling (2024-01-24)
期刊：Journal of Integrative Medicine-JIM
DOI：10.1016/j.joim.2024.01.003
影响因子 ：4.8
引用产品： 3T3-L1 细胞
-
STUB1 increases adiponectin expression by inducing ubiquitination and degradation of NR2F2, thereby reducing hepatic stellate cell activation and alleviating non-alcoholic fatty liver disease (2024-03-02)
期刊：TISSUE & CELL
DOI：10.1016/j.tice.2024.102345
影响因子 ：2.6
引用产品： 3T3-L1 细胞 , 3T3-L1细胞专用培养基
-
Metformin plus L-carnitine enhances brown/beige adipose tissue activity via Nrf2/HO-1 signaling to reduce lipid accumulation and inflammation in murine obesity (2024-02-13)
期刊：Open Medicine
影响因子 ：2.1
引用产品： 3T3-L1 细胞 , DMEM高糖 培养基 , 青霉素-链霉素溶液 (双抗)，100×
-
High-throughput single nucleus total RNA sequencing of formalin-fixed paraffin-embedded tissues by snRandom-seq (2023-05-12)
期刊：Nature Communications
DOI：10.1038/s41467-023-38409-5
影响因子 ：16.6
引用产品： 293T [HEK-293T] 细胞 , 3T3-L1 细胞
-
Effective Treatment of Haemophilus influenzae-Induced Bacterial Conjunctivitis by a Bioadhesive Nanoparticle Reticulate Structure (2023-05-08)
期刊：ACS Applied Materials & Interfaces
影响因子 ：9.5
引用产品： 3T3-L1 细胞
-
Quercitrin alleviates lipid metabolism disorder in polycystic ovary syndrome-insulin resistance by upregulating PM20D1 in the PI3K/Akt pathway (2023-06-02)
期刊：PHYTOMEDICINE
DOI：10.1016/j.phymed.2023.154908
影响因子 ：7.9
引用产品： 3T3-L1 细胞
-
Lignans from Patrinia scabiosaefolia improve insulin resistance by activating PI-3K/AKT pathway and promoting GLUT4 expression (2023-04-04)
期刊：Food Science and Human Wellness
DOI：10.1016/j.fshw.2023.03.015
影响因子 ：7.0
引用产品： 3T3-L1 细胞
-
Dual Regulation of Sulfonated Lignin to Prevent and Treat Type 2 Diabetes Mellitus (2023-01-06)
期刊：BIOMACROMOLECULES
DOI：10.1021/acs.biomac.2c01267
影响因子 ：6.2
引用产品： MCF7 [MCF-7] 细胞 , 3T3-L1 细胞 , Hep G2 细胞
-
MCP-1-Loaded Poly(l-lactide-co-caprolactone) Fibrous Films Modulate Macrophage Polarization toward an Anti-inflammatory Phenotype and Improve Angiogenesis (2023-06-27)
期刊：ACS Biomaterials Science & Engineering
DOI：10.1021/acsbiomaterials.3c00476
影响因子 ：5.8
引用产品： RAW 264.7 细胞 , 3T3-L1 细胞 , SVEC4-10 细胞
-
Baicalein enhances immune response in TNBC by inhibiting leptin expression of adipocytes (2023-07-25)
期刊：CANCER SCIENCE
影响因子 ：5.7
引用产品： 3T3-L1 细胞
-
Mulberry leaf flavonoids activate BAT and induce browning of WAT to improve type 2 diabetes via regulating the AMPK/SIRT1/PGC-1α signaling pathway (2023-11-28)
期刊：Chinese Journal of Natural Medicines
DOI：10.1016/S1875-5364(23)60481-9
影响因子 ：4.6
引用产品： 3T3-L1 细胞
-
Delivery of microRNA-423-5p by exosome from adipose-derived stem/stromal cells inhibits DVL3 to potentiate autologous fat graft survival through adipogenesis and inflammatory response (2023-12-02)
期刊：Human Cell
DOI：10.1007/s13577-023-01010-5
影响因子 ：4.3
引用产品： 人脂肪间充质干细胞 , 人脂肪间充质干细胞完全培养基 , 3T3-L1 细胞
-
CAA-derived IL-6 induced M2 macrophage polarization by activating STAT3 (2023-05-01)
期刊：BMC CANCER
DOI：10.1186/s12885-023-10826-1
影响因子 ：3.8
引用产品： 3T3-L1 细胞 , MDA-MB-231 细胞 , MCF7 [MCF-7] 细胞
-
Breast cancer cell-derived exosome-delivered microRNA-155 targets UBQLN1 in adipocytes and facilitates cancer cachexia-related fat loss (2023-04-05)
期刊：HUMAN MOLECULAR GENETICS
影响因子 ：3.5
引用产品： 3T3-L1 细胞
-
New iridoids from Patrinia scabiosaefolia and their hypoglycemic effects by activating PI3K/Akt signaling pathway (2023-01-03)
期刊：FITOTERAPIA
DOI：10.1016/j.fitote.2022.105423
影响因子 ：3.4
引用产品： 3T3-L1 细胞
-
Bovine C-X-C Motif Chemokine Ligand 14 Expression Is Regulated by Alternative Polyadenylation and MicroRNAs (2023-09-30)
期刊：Animals
影响因子 ：3.0
引用产品： 293T [HEK-293T] 细胞 , 3T3-L1 细胞
-
Effects of the CDC10 (Septin 7) Gene on the Proliferation and Differentiation of Bovine Intramuscular Preadipocyte and 3T3-L1 Cells (2023-02-09)
期刊：Animals
影响因子 ：3.0
引用产品： 3T3-L1 细胞
-
Isolation and extraction of glansreginin A from walnut meal and its effect on the proliferation of 3T3-L1 cells (2023-04-03)
期刊：Food Science and Technology
影响因子 ：2.6
引用产品： 3T3-L1 细胞
-
miR-34a Regulates Lipid Droplet Deposition in 3T3-L1 and C2C12 Cells by Targeting LEF1 (2022-12-30)
期刊：Cells
影响因子 ：7.7
引用产品： 3T3-L1 细胞 , C2C12 细胞 , 293 [HEK-293] 细胞
-
6-Shogaol Exhibits a Promoting Effect with Tax via Binding HSP60 in Non-Small-Cell Lung Cancer (2022-11-19)
期刊：Cells
影响因子 ：7.7
引用产品： A-375 细胞 , A549 [A-549] 细胞 , H8 细胞 , U251 细胞 , 3T3-L1 细胞 , PANC-1 细胞 , TSSCA 细胞 , HK-2 细胞 , DMEM高糖 培养基 , RPMI-1640 培养基
-
A confined crosslinking strategy towards an intelligent organosilica–micellar hybrid drug delivery system (2001-02-20)
期刊：Biomaterials Science
影响因子 ：6.6
引用产品： SMMC-7721 细胞 , NIH/3T3 细胞 , 3T3-L1 细胞 , Hela 细胞
-
Quinacrine attenuates diet-induced obesity by inhibiting adipogenesis via activation of AMPK signaling (2022-11-30)
期刊：EUROPEAN JOURNAL OF PHARMACOLOGY
DOI：10.1016/j.ejphar.2022.175432
影响因子 ：5.2
引用产品： 3T3-L1 细胞
-
Development of a Self-Assembled Hydrogels Based on Carboxymethyl Chitosan and Oxidized Hyaluronic Acid Containing Tanshinone Extract Nanocrystals for Enhanced Dissolution and Acne Treatment (2022-12-09)
期刊：Pharmaceuticals
影响因子 ：5.2
引用产品： 3T3-L1 细胞 , 增强型细胞活力检测试剂盒 (CCK-8)
-
Potentilla bifurca flavonoids effectively improve insulin resistance (2022-11-01)
期刊：European Review for Medical and Pharmacological Sciences
DOI：10.26355/eurrev_202211_30370
影响因子 ：3.8
引用产品： 3T3-L1 细胞 , DMEM高糖 培养基 , 青霉素-链霉素溶液 (双抗)，100× , 磷酸盐缓冲液 (PBS) 1× , 0.25%胰蛋白酶溶液 (含EDTA，溶于PBS) , 重组人胰岛素溶液 (10mg/mL)
-
Salvianolic acid A promotes mitochondrial biogenesis and mitochondrial function in 3T3-L1 adipocytes through regulation of the AMPK-PGC1α signalling pathway (2022-09-02)
期刊：Adipocyte
DOI：10.1080/21623945.2022.2116790
影响因子 ：3.6
引用产品： 3T3-L1 细胞
-
Circadian rhythm disorders elevate macrophages cytokines release and promote multiple tissues/organs dysfunction in mice (2003-02-20)
期刊：Physiol Behav
DOI：10.1016/j.physbeh.2022.113772
影响因子 ：2.9
-
Shortening of HO1 3′UTRs by Alternative Polyadenylation Suppresses Adipogenesis in 3T3-L1 (2007-01-20)
期刊：Journal Of Agricultural And Food Chemistry
影响因子 ：6.1
引用产品： 3T3-L1 细胞
-
Blood cell repelling and tumor cell capturing surface for high-purity enrichment of circulating tumor cells (2010-09-20)
期刊：Journal of Materials Chemistry B
影响因子 ：7.6
引用产品： NCTC clone 929 [L 细胞, L-929] 细胞 , 3T3-L1 细胞 , MCF7 [MCF-7] 细胞 , Hela 细胞
-
Icariin Inhibits AMPK-Dependent Autophagy and Adipogenesis in Adipocytes In vitro and in a Model of Graves Orbitopathy In vivo (2002-07-20)
期刊：Frontiers in Physiology
影响因子 ：4.0
引用产品： 3T3-L1 细胞
-
Chenodeoxycholic acid attenuates high‑fat diet‑induced obesity and hyperglycemia via the G protein‑coupled bile acid receptor 1 and proliferator‑activated receptor γ pathway (2010-07-20)
期刊：Experimental and Therapeutic Medicine
影响因子 ：2.7
引用产品： 3T3-L1 细胞
FAQs
Q：{{item.question}}
A：
产品资料
识别码示意图
