产品中心Cell Resources
联系我们CONTACT US
400-999-210024小时服务热线
产品概述
| 名称 | HCT-15 (人结直肠腺癌细胞) (STR鉴定正确) |
| 别称 | HCT-15; HCT15 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:8 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | DNA指纹鉴定证据表明，HCT-15细胞和DLD-1细胞来源于同一个人；但同工酶及细胞染色体组型分析仍存疑问。HCT-15细胞呈CSAp阴性(CSAp-)；HCT-15角蛋白免疫过氧化物酶染色阳性。 |
| 年龄（性别） | 男性 |
| 组织来源 | 结直肠腺癌 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肠癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~20-25 hours |
| 致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
| 基因表达情况 | carcinoembryonic antigen (CEA) 5.4 ng/10^6 cells/10 days. The cells are positive for keratin by immunoperoxidase staining. |
| 保藏机构 | ATCC; CCL-225 BCRC; 60539 DSMZ; ACC-357 ECACC; 91030712 |
STR鉴定
-
STR位点信息
Amelogenin X,Y CSF1PO 12 D2S1338 17,25 D3S1358 17 D5S818 13 D7S820 10,12 D8S1179 15 D13S317 8,11 D16S539 12,13 D18S51 11,17 D19S433 14,16 D21S11 29,32.2 FGA 22 PentaD 9,14 PentaE 7,14 TH01 7,9.3 TPOX 8,11 vWA 18,19 D6S1043 11,13 D12S391 19,22 D2S441 13,15 -
STR鉴定图
-
参考文献
-
Design, Synthesis, and Antitumor Activity Evaluation of Novel VISTA Small Molecule Inhibitors (2024-02-27)
作者：Chengliang Sun:1.State Key Laboratory of Natural Medicines and Jiangsu Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing 210009, China. ; 2.Department of Medicinal Chemistry, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, China. ; 3.Institute of Innovative Drug Discovery and Development, China Pharmaceutical University, Nanjing 211198, China. ; Yuling He:1.State Key Laboratory of Natural Medicines and Jiangsu Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing 210009, China. ; 2.Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, China. ; Gefei Wang:1.State Key Laboratory of Natural Medicines and Jiangsu Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing 210009, China. ; 2.Department of Medicinal Chemistry, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, China. ; 3.Institute of Innovat
期刊：Design, Synthesis, and Antitumor Activity Evaluation of Novel VISTA Small Molecule Inhibitors
DOI：10.1021/acs.jmedchem.3c02039
影响因子 ：7.3
引用产品： SK-OV-3 细胞 , HCT-15 细胞 , CHO 细胞
-
Discovery of Novel 4-Hydroxyquinazoline Derivatives: In Silico, In Vivo and In Vitro Studies Using Primary PARPi-Resistant Cell Lines (2024-03-21)
作者：Lijie Zhu:1.School of Pharmacy, Shandong Second Medical University, Weifang 261053, China. ; Binzhuo Liu:1.School of Pharmacy, Shandong Second Medical University, Weifang 261053, China. ; Feng Jin:1.School of Pharmacy, Shandong Second Medical University, Weifang 261053, China. ; Weilong Cao:1.School of Pharmacy, Shandong Second Medical University, Weifang 261053, China. ; Guangzhao Xu:1.School of Pharmacy, Shandong Second Medical University, Weifang 261053, China. ; Xinwei Zhang:1.School of Pharmacy, Shandong Second Medical University, Weifang 261053, China. ; Peng Peng:1.School of Pharmacy (Preparatory), East China Normal University, Shanghai 200241, China. ; Dingding Gao:1.The Research Center of Chiral Drugs, Innovation Research Institute of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. ; Bin Wang:1.School of Pharmacy, Shandong Second Medical University, Weifang 261053, China. ; Kairui Feng:1.School of Pharmacy, Sh
期刊：Discovery of Novel 4-Hydroxyquinazoline Derivatives: In Silico, In Vivo and In Vitro Studies Using Primary PARPi-Resistant Cell Lines
影响因子 ：4.6
引用产品： HCT-15 细胞 , HCC1937 细胞 , MDA-MB-231 细胞 , HL-7702 [L-02; LO2] 细胞 , NCM460 细胞
-
Elevated expression of the rhythm gene NFIL3 promotes the progression of TNBC by activating NF-κB signaling through suppression of NFKBIA transcription (2002-02-20)
作者：Weiwei Yang:1.Department of Pathology, Harbin Medical University, Harbin, China. ; Jing Li:1.Department of Pathology, Harbin Medical University, Harbin, China. ; 2.Electronic Microscope Center of Harbin Medical University, Harbin, China. ; Minghui Zhang:1.Department of Oncology, Chifeng City Hospital, Chifeng, China. ; Haichuan Yu:1.School of Medical Laboratory, Xinxiang Medical University, Xinxiang, China. ; Yuan Zhuang:1.Department of Pathology, Harbin Medical University, Harbin, China. ; Lingyu Zhao:1.Department of Pathology, Harbin Medical University, Harbin, China. ; Lili Ren:1.Department of Pathology, Harbin Medical University, Harbin, China. ; Jinan Gong:1.Clinicopathological Diagnosis Center, Qiqihar Medical University, Qiqihar, China. ; Hongjie Bi:1.Department of Pathology, Harbin Medical University, Harbin, China. ; Lixuan Zeng:1.Department of Pathology, Harbin Medical University, Harbin, China. ; Yang Xue:1.Department of Pathology, Harbin Medical University, Harbin
期刊：Elevated expression of the rhythm gene NFIL3 promotes the progression of TNBC by activating NF-κB signaling through suppression of NFKBIA transcription
DOI：10.1186/s13046-022-02260-1
影响因子 ：11.3
引用产品： Hs 578T 细胞 , BT-549 细胞 , HCT-15 细胞 , MCF 10A 细胞 , A549 [A-549] 细胞 , SK-OV-3 细胞 , Hela 细胞 , PANC-1 细胞
-
Identification of KIF21B as a Biomarker for Colorectal Cancer and Associated with Poor Prognosis (2022-11-21)
作者：Shanshan Xu:1.Major of Chinese Medicine Surgery, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210000, China. ; Youran Li:1.Department of Colorectal Surgery, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210000, China. ; Hua Huang:1.Department of Anorectal, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu 215500, China. ; Xian Miao:1.Department of Oncology, Nantong Hospital of Traditional Chinese, Nantong, Jiangsu 226001, China. ; Yunfei Gu:1.Department of Colorectal Surgery, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210000, China. ;
期刊：Identification of KIF21B as a Biomarker for Colorectal Cancer and Associated with Poor Prognosis
影响因子 ：4.5
引用产品： NCM460 细胞 , HT-29 细胞 , HCT 116 细胞 , SW48 细胞 , HCT-15 细胞
-
CircFAM120B Blocks the Development of Colorectal Cancer by Activating TGF-Beta Receptor II Expression via Targeting miR-645 (2007-01-20)
作者：You Yu:1.Department of General Surgery, Chongqing Bishan People's Hospital, Chongqing, China. ; Xiao Lei:1.Department of General Surgery, The First Hospital Affiliated to AMU (Southwest Hospital), Chongqing, China. ;
期刊：CircFAM120B Blocks the Development of Colorectal Cancer by Activating TGF-Beta Receptor II Expression via Targeting miR-645
影响因子 ：5.5
引用产品： LoVo 细胞 , HCT-15 细胞 , RPMI-1640 (含双抗) 培养基
-
LncRNA ENSG00000254615 Modulates Proliferation and 5-FU Resistance by Regulating p21 and Cyclin D1 in Colorectal Cancer (2005-01-20)
作者：Yang Fu:1.Department of Medical Oncology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China. ; 2.Guangdong Research Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China. ; Runqing Huang:1.Department of Medical Oncology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China. ; 2.Guangdong Research Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China. ; 3.Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China. ; Jianxia Li:1.Department of Medical Oncology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China. ; 2.Guangdong Research Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China. ; Xiaoyu Xie:1.Department of Medical Oncology, The Sixth Affiliated Hospital, Sun Y
期刊：LncRNA ENSG00000254615 Modulates Proliferation and 5-FU Resistance by Regulating p21 and Cyclin D1 in Colorectal Cancer
DOI：10.1080/07357907.2021.1923727
影响因子 ：2.4
引用产品： HCT-15 细胞
FAQs
Q：{{item.question}}
A：
产品资料
识别码示意图
